Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09460 and EX31_RS09465 are separated by 14 nucleotides EX31_RS09465 and EX31_RS09470 overlap by 26 nucleotides EX31_RS09470 and EX31_RS09475 are separated by 212 nucleotides
EX31_RS09460: EX31_RS09460 - flagellar filament capping protein FliD, at 102,000 to 103,436
_RS09460
EX31_RS09465: EX31_RS09465 - flagellar export chaperone FliS, at 103,451 to 103,888
_RS09465
EX31_RS09470: EX31_RS09470 - flagella biosynthesis regulatory protein FliT, at 103,863 to 104,225
_RS09470
EX31_RS09475: EX31_RS09475 - AraC family transcriptional regulator, at 104,438 to 105,292
_RS09475
Position (kb) 103
104 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 102.546 kb on + strand, within EX31_RS09460 at 102.629 kb on + strand, within EX31_RS09460 at 102.640 kb on - strand, within EX31_RS09460 at 102.641 kb on + strand, within EX31_RS09460 at 102.641 kb on + strand, within EX31_RS09460 at 102.641 kb on + strand, within EX31_RS09460 at 102.641 kb on + strand, within EX31_RS09460 at 102.764 kb on + strand, within EX31_RS09460 at 102.821 kb on + strand, within EX31_RS09460 at 102.822 kb on - strand, within EX31_RS09460 at 102.864 kb on + strand, within EX31_RS09460 at 102.864 kb on + strand, within EX31_RS09460 at 102.864 kb on + strand, within EX31_RS09460 at 102.865 kb on - strand, within EX31_RS09460 at 102.881 kb on + strand, within EX31_RS09460 at 102.881 kb on + strand, within EX31_RS09460 at 103.058 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.229 kb on + strand, within EX31_RS09460 at 103.287 kb on + strand, within EX31_RS09460 at 103.287 kb on + strand, within EX31_RS09460 at 103.287 kb on + strand, within EX31_RS09460 at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.413 kb on - strand at 103.413 kb on - strand at 103.435 kb on - strand at 103.437 kb on + strand at 103.438 kb on - strand at 103.450 kb on + strand at 103.450 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.455 kb on - strand at 103.455 kb on - strand at 103.456 kb on + strand at 103.456 kb on + strand at 103.456 kb on + strand at 103.456 kb on + strand at 103.457 kb on - strand at 103.478 kb on + strand at 103.579 kb on + strand, within EX31_RS09465 at 103.579 kb on + strand, within EX31_RS09465 at 103.668 kb on + strand, within EX31_RS09465 at 103.668 kb on + strand, within EX31_RS09465 at 103.695 kb on + strand, within EX31_RS09465 at 103.718 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.725 kb on - strand, within EX31_RS09465 at 103.725 kb on - strand, within EX31_RS09465 at 103.727 kb on - strand, within EX31_RS09465 at 103.838 kb on + strand, within EX31_RS09465 at 103.839 kb on - strand, within EX31_RS09465 at 103.839 kb on - strand, within EX31_RS09465 at 103.839 kb on - strand, within EX31_RS09465 at 103.859 kb on + strand at 103.861 kb on + strand at 103.861 kb on + strand at 103.878 kb on + strand at 103.878 kb on + strand at 103.878 kb on + strand at 103.980 kb on + strand, within EX31_RS09470 at 103.980 kb on + strand, within EX31_RS09470 at 103.980 kb on + strand, within EX31_RS09470 at 103.981 kb on - strand, within EX31_RS09470 at 104.072 kb on + strand, within EX31_RS09470 at 104.172 kb on - strand, within EX31_RS09470 at 104.178 kb on + strand, within EX31_RS09470 at 104.179 kb on - strand, within EX31_RS09470 at 104.179 kb on - strand, within EX31_RS09470 at 104.256 kb on + strand at 104.311 kb on + strand at 104.311 kb on + strand at 104.312 kb on - strand at 104.338 kb on + strand at 104.338 kb on + strand at 104.338 kb on + strand at 104.338 kb on + strand at 104.338 kb on + strand at 104.344 kb on + strand at 104.344 kb on + strand at 104.344 kb on + strand at 104.344 kb on + strand at 104.344 kb on + strand at 104.344 kb on + strand at 104.368 kb on + strand at 104.402 kb on + strand at 104.402 kb on + strand at 104.403 kb on - strand at 104.446 kb on + strand at 104.522 kb on + strand at 104.522 kb on + strand at 104.523 kb on - strand at 104.523 kb on - strand at 104.523 kb on - strand at 104.525 kb on - strand, within EX31_RS09475 at 104.525 kb on - strand, within EX31_RS09475 at 104.525 kb on - strand, within EX31_RS09475 at 104.710 kb on - strand, within EX31_RS09475 at 104.764 kb on + strand, within EX31_RS09475
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 102,546 + EX31_RS09460 0.38 -0.5 102,629 + EX31_RS09460 0.44 -2.8 102,640 - EX31_RS09460 0.45 -1.1 102,641 + EX31_RS09460 0.45 -0.1 102,641 + EX31_RS09460 0.45 -0.0 102,641 + EX31_RS09460 0.45 -0.7 102,641 + EX31_RS09460 0.45 +0.3 102,764 + EX31_RS09460 0.53 +1.9 102,821 + EX31_RS09460 0.57 +0.3 102,822 - EX31_RS09460 0.57 -1.1 102,864 + EX31_RS09460 0.60 -0.7 102,864 + EX31_RS09460 0.60 -0.5 102,864 + EX31_RS09460 0.60 -1.1 102,865 - EX31_RS09460 0.60 -1.1 102,881 + EX31_RS09460 0.61 -1.1 102,881 + EX31_RS09460 0.61 -1.6 103,058 + EX31_RS09460 0.74 +0.0 103,153 + EX31_RS09460 0.80 -2.1 103,153 + EX31_RS09460 0.80 +0.0 103,153 + EX31_RS09460 0.80 -0.1 103,153 + EX31_RS09460 0.80 +0.3 103,153 + EX31_RS09460 0.80 +1.5 103,153 + EX31_RS09460 0.80 +0.3 103,229 + EX31_RS09460 0.86 +0.0 103,287 + EX31_RS09460 0.90 -0.6 103,287 + EX31_RS09460 0.90 -0.9 103,287 + EX31_RS09460 0.90 +0.9 103,412 + -0.3 103,412 + +0.5 103,412 + +0.3 103,412 + +0.1 103,412 + -1.0 103,412 + +0.8 103,412 + -2.1 103,412 + -1.1 103,413 - +0.3 103,413 - -1.3 103,435 - -0.0 103,437 + +1.5 103,438 - -1.8 103,450 + +0.0 103,450 + -1.6 103,454 + -0.7 103,454 + -3.0 103,454 + -1.3 103,454 + -2.0 103,454 + +0.0 103,454 + -1.0 103,454 + -0.8 103,454 + -1.0 103,454 + +0.2 103,454 + -2.1 103,454 + -0.7 103,454 + -1.3 103,454 + +0.3 103,454 + -1.4 103,455 - -0.5 103,455 - -1.0 103,456 + -0.4 103,456 + +0.9 103,456 + -2.6 103,456 + -1.4 103,457 - +0.3 103,478 + -1.3 103,579 + EX31_RS09465 0.29 -0.5 103,579 + EX31_RS09465 0.29 +1.4 103,668 + EX31_RS09465 0.50 -0.6 103,668 + EX31_RS09465 0.50 +1.9 103,695 + EX31_RS09465 0.56 +0.2 103,718 + EX31_RS09465 0.61 +0.5 103,724 + EX31_RS09465 0.62 -1.4 103,724 + EX31_RS09465 0.62 -0.3 103,724 + EX31_RS09465 0.62 -1.1 103,724 + EX31_RS09465 0.62 +1.3 103,724 + EX31_RS09465 0.62 -2.6 103,724 + EX31_RS09465 0.62 -1.2 103,724 + EX31_RS09465 0.62 -2.5 103,725 - EX31_RS09465 0.63 -0.9 103,725 - EX31_RS09465 0.63 -0.7 103,727 - EX31_RS09465 0.63 -1.0 103,838 + EX31_RS09465 0.88 -1.1 103,839 - EX31_RS09465 0.89 -1.1 103,839 - EX31_RS09465 0.89 -0.7 103,839 - EX31_RS09465 0.89 +0.3 103,859 + -0.3 103,861 + -0.2 103,861 + +0.5 103,878 + -0.3 103,878 + -0.1 103,878 + -1.5 103,980 + EX31_RS09470 0.32 +0.9 103,980 + EX31_RS09470 0.32 +0.5 103,980 + EX31_RS09470 0.32 -0.9 103,981 - EX31_RS09470 0.33 +0.9 104,072 + EX31_RS09470 0.58 +0.0 104,172 - EX31_RS09470 0.85 +1.3 104,178 + EX31_RS09470 0.87 -0.4 104,179 - EX31_RS09470 0.87 -1.0 104,179 - EX31_RS09470 0.87 -0.7 104,256 + +1.9 104,311 + +0.1 104,311 + +0.0 104,312 - +1.3 104,338 + +0.0 104,338 + +0.3 104,338 + +0.5 104,338 + +0.2 104,338 + -0.6 104,344 + -0.1 104,344 + -0.5 104,344 + +0.3 104,344 + -0.5 104,344 + +1.9 104,344 + -0.3 104,368 + +0.2 104,402 + +1.3 104,402 + -0.4 104,403 - +1.5 104,446 + +1.4 104,522 + -0.5 104,522 + -1.4 104,523 - +1.3 104,523 - +0.3 104,523 - -0.0 104,525 - EX31_RS09475 0.10 +0.9 104,525 - EX31_RS09475 0.10 -0.5 104,525 - EX31_RS09475 0.10 -0.1 104,710 - EX31_RS09475 0.32 -1.0 104,764 + EX31_RS09475 0.38 +2.9
Or see this region's nucleotide sequence