Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07375 and EX31_RS07380 are separated by 218 nucleotides EX31_RS07380 and EX31_RS07385 are separated by 173 nucleotides EX31_RS07385 and EX31_RS07390 are separated by 210 nucleotides
EX31_RS07375: EX31_RS07375 - SulP family inorganic anion transporter, at 278,511 to 279,992
_RS07375
EX31_RS07380: EX31_RS07380 - redox-regulated ATPase YchF, at 280,211 to 281,302
_RS07380
EX31_RS07385: EX31_RS07385 - HNH nuclease family protein, at 281,476 to 281,823
_RS07385
EX31_RS07390: EX31_RS07390 - TonB-dependent siderophore receptor, at 282,034 to 284,241
_RS07390
Position (kb)
280
281
282 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 279.319 kb on - strand, within EX31_RS07375 at 279.408 kb on - strand, within EX31_RS07375 at 279.557 kb on - strand, within EX31_RS07375 at 279.557 kb on - strand, within EX31_RS07375 at 279.603 kb on - strand, within EX31_RS07375 at 279.603 kb on - strand, within EX31_RS07375 at 279.603 kb on - strand, within EX31_RS07375 at 279.734 kb on - strand, within EX31_RS07375 at 279.992 kb on + strand at 279.993 kb on - strand at 279.993 kb on - strand at 279.993 kb on - strand at 280.039 kb on - strand at 280.079 kb on - strand at 280.130 kb on + strand at 280.130 kb on + strand at 280.130 kb on + strand at 280.154 kb on + strand at 280.154 kb on + strand at 280.154 kb on + strand at 280.155 kb on - strand at 280.155 kb on - strand at 280.188 kb on + strand at 280.383 kb on + strand, within EX31_RS07380 at 280.453 kb on + strand, within EX31_RS07380 at 280.820 kb on + strand, within EX31_RS07380 at 280.820 kb on + strand, within EX31_RS07380 at 280.820 kb on + strand, within EX31_RS07380 at 280.820 kb on + strand, within EX31_RS07380 at 281.198 kb on + strand at 281.198 kb on + strand at 281.198 kb on + strand at 281.255 kb on + strand at 281.255 kb on + strand at 281.373 kb on + strand at 281.398 kb on + strand at 281.419 kb on - strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.427 kb on + strand at 281.428 kb on - strand at 281.428 kb on - strand at 281.428 kb on - strand at 281.428 kb on - strand at 281.428 kb on - strand at 281.428 kb on - strand at 281.428 kb on - strand at 281.428 kb on - strand at 281.431 kb on - strand at 281.459 kb on + strand at 281.628 kb on + strand, within EX31_RS07385 at 281.629 kb on - strand, within EX31_RS07385 at 281.634 kb on + strand, within EX31_RS07385 at 281.652 kb on + strand, within EX31_RS07385 at 281.653 kb on - strand, within EX31_RS07385 at 281.674 kb on + strand, within EX31_RS07385 at 281.674 kb on + strand, within EX31_RS07385 at 281.701 kb on + strand, within EX31_RS07385 at 281.701 kb on + strand, within EX31_RS07385 at 281.776 kb on + strand, within EX31_RS07385 at 281.776 kb on + strand, within EX31_RS07385 at 281.776 kb on + strand, within EX31_RS07385 at 281.777 kb on - strand, within EX31_RS07385 at 281.777 kb on - strand, within EX31_RS07385 at 281.777 kb on - strand, within EX31_RS07385 at 281.795 kb on + strand at 281.821 kb on + strand at 281.821 kb on + strand at 281.822 kb on - strand at 281.930 kb on + strand at 281.930 kb on + strand at 281.930 kb on + strand at 281.930 kb on + strand at 281.930 kb on + strand at 281.931 kb on - strand at 281.931 kb on - strand at 281.931 kb on - strand at 281.931 kb on - strand at 281.931 kb on - strand at 281.931 kb on - strand at 281.931 kb on - strand at 282.033 kb on + strand at 282.034 kb on - strand at 282.034 kb on - strand at 282.034 kb on - strand at 282.095 kb on + strand at 282.096 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 279,319 - EX31_RS07375 0.55 +0.7 279,408 - EX31_RS07375 0.61 -0.7 279,557 - EX31_RS07375 0.71 -0.3 279,557 - EX31_RS07375 0.71 -1.0 279,603 - EX31_RS07375 0.74 +0.1 279,603 - EX31_RS07375 0.74 +1.8 279,603 - EX31_RS07375 0.74 +0.2 279,734 - EX31_RS07375 0.83 -0.3 279,992 + +0.3 279,993 - +0.3 279,993 - +0.7 279,993 - +1.1 280,039 - +3.5 280,079 - +1.7 280,130 + +1.7 280,130 + -0.0 280,130 + +0.1 280,154 + +1.7 280,154 + +0.8 280,154 + +0.7 280,155 - +0.7 280,155 - +1.1 280,188 + -0.9 280,383 + EX31_RS07380 0.16 -0.9 280,453 + EX31_RS07380 0.22 +1.1 280,820 + EX31_RS07380 0.56 -0.9 280,820 + EX31_RS07380 0.56 -1.1 280,820 + EX31_RS07380 0.56 -2.0 280,820 + EX31_RS07380 0.56 +0.3 281,198 + -0.9 281,198 + +1.1 281,198 + +1.0 281,255 + +1.7 281,255 + -0.3 281,373 + +1.1 281,398 + -0.2 281,419 - -0.0 281,427 + +0.1 281,427 + +1.7 281,427 + -0.8 281,427 + -0.1 281,427 + -0.3 281,427 + -0.5 281,427 + -2.2 281,427 + +0.1 281,427 + +0.7 281,427 + -0.1 281,427 + -0.9 281,427 + +0.1 281,427 + -0.6 281,427 + -0.0 281,427 + +0.0 281,427 + +0.7 281,428 - -0.2 281,428 - -0.6 281,428 - -0.7 281,428 - +0.9 281,428 - -0.3 281,428 - -0.1 281,428 - +0.7 281,428 - -1.0 281,431 - +2.5 281,459 + +0.8 281,628 + EX31_RS07385 0.44 -0.7 281,629 - EX31_RS07385 0.44 -0.8 281,634 + EX31_RS07385 0.45 -0.3 281,652 + EX31_RS07385 0.51 +1.5 281,653 - EX31_RS07385 0.51 +0.7 281,674 + EX31_RS07385 0.57 +0.7 281,674 + EX31_RS07385 0.57 +1.2 281,701 + EX31_RS07385 0.65 +1.3 281,701 + EX31_RS07385 0.65 +0.5 281,776 + EX31_RS07385 0.86 -0.4 281,776 + EX31_RS07385 0.86 -0.6 281,776 + EX31_RS07385 0.86 +1.1 281,777 - EX31_RS07385 0.86 +0.7 281,777 - EX31_RS07385 0.86 -0.7 281,777 - EX31_RS07385 0.86 -0.7 281,795 + +3.3 281,821 + +0.1 281,821 + -1.3 281,822 - +0.3 281,930 + -1.3 281,930 + -0.2 281,930 + +0.7 281,930 + +1.2 281,930 + +1.3 281,931 - +0.3 281,931 - -1.7 281,931 - -0.1 281,931 - +1.7 281,931 - -1.9 281,931 - +1.7 281,931 - -0.7 282,033 + -0.2 282,034 - +1.7 282,034 - -0.1 282,034 - +1.7 282,095 + +0.4 282,096 - -1.1
Or see this region's nucleotide sequence