Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06990 and EX31_RS06995 are separated by 265 nucleotides EX31_RS06995 and EX31_RS07000 are separated by 38 nucleotides EX31_RS07000 and EX31_RS07005 are separated by 19 nucleotides
EX31_RS06990: EX31_RS06990 - GntR family transcriptional regulator, at 187,223 to 187,918
_RS06990
EX31_RS06995: EX31_RS06995 - hypothetical protein, at 188,184 to 188,792
_RS06995
EX31_RS07000: EX31_RS07000 - iron ABC transporter permease, at 188,831 to 190,561
_RS07000
EX31_RS07005: EX31_RS07005 - ABC transporter substrate-binding protein, at 190,581 to 191,579
_RS07005
Position (kb) 188
189
190
191 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 187.913 kb on - strand at 187.961 kb on - strand at 187.990 kb on - strand at 187.990 kb on - strand at 188.039 kb on + strand at 188.247 kb on - strand, within EX31_RS06995 at 188.278 kb on + strand, within EX31_RS06995 at 188.279 kb on - strand, within EX31_RS06995 at 188.283 kb on - strand, within EX31_RS06995 at 188.396 kb on + strand, within EX31_RS06995 at 188.396 kb on + strand, within EX31_RS06995 at 188.396 kb on + strand, within EX31_RS06995 at 188.397 kb on - strand, within EX31_RS06995 at 188.397 kb on - strand, within EX31_RS06995 at 188.432 kb on + strand, within EX31_RS06995 at 188.484 kb on + strand, within EX31_RS06995 at 188.484 kb on + strand, within EX31_RS06995 at 188.485 kb on - strand, within EX31_RS06995 at 188.485 kb on - strand, within EX31_RS06995 at 188.509 kb on - strand, within EX31_RS06995 at 188.509 kb on - strand, within EX31_RS06995 at 188.509 kb on - strand, within EX31_RS06995 at 188.589 kb on + strand, within EX31_RS06995 at 188.589 kb on + strand, within EX31_RS06995 at 188.589 kb on + strand, within EX31_RS06995 at 188.589 kb on + strand, within EX31_RS06995 at 188.590 kb on - strand, within EX31_RS06995 at 188.591 kb on + strand, within EX31_RS06995 at 188.591 kb on + strand, within EX31_RS06995 at 188.645 kb on + strand, within EX31_RS06995 at 188.645 kb on + strand, within EX31_RS06995 at 188.646 kb on - strand, within EX31_RS06995 at 188.662 kb on + strand, within EX31_RS06995 at 188.662 kb on + strand, within EX31_RS06995 at 188.663 kb on - strand, within EX31_RS06995 at 188.676 kb on + strand, within EX31_RS06995 at 188.680 kb on + strand, within EX31_RS06995 at 188.680 kb on + strand, within EX31_RS06995 at 188.849 kb on + strand at 189.098 kb on + strand, within EX31_RS07000 at 189.098 kb on + strand, within EX31_RS07000 at 189.098 kb on + strand, within EX31_RS07000 at 189.099 kb on - strand, within EX31_RS07000 at 189.099 kb on - strand, within EX31_RS07000 at 189.099 kb on - strand, within EX31_RS07000 at 189.099 kb on - strand, within EX31_RS07000 at 189.099 kb on - strand, within EX31_RS07000 at 189.099 kb on - strand, within EX31_RS07000 at 189.101 kb on - strand, within EX31_RS07000 at 189.209 kb on - strand, within EX31_RS07000 at 189.222 kb on + strand, within EX31_RS07000 at 189.223 kb on - strand, within EX31_RS07000 at 189.362 kb on - strand, within EX31_RS07000 at 189.463 kb on + strand, within EX31_RS07000 at 189.507 kb on + strand, within EX31_RS07000 at 189.508 kb on - strand, within EX31_RS07000 at 189.743 kb on - strand, within EX31_RS07000 at 189.817 kb on - strand, within EX31_RS07000 at 190.259 kb on - strand, within EX31_RS07000 at 190.337 kb on - strand, within EX31_RS07000 at 190.374 kb on - strand, within EX31_RS07000 at 190.457 kb on + strand at 190.799 kb on + strand, within EX31_RS07005 at 190.799 kb on + strand, within EX31_RS07005 at 190.799 kb on + strand, within EX31_RS07005 at 190.800 kb on - strand, within EX31_RS07005 at 190.800 kb on - strand, within EX31_RS07005 at 190.800 kb on - strand, within EX31_RS07005 at 190.890 kb on - strand, within EX31_RS07005 at 190.924 kb on + strand, within EX31_RS07005 at 191.135 kb on + strand, within EX31_RS07005 at 191.156 kb on + strand, within EX31_RS07005 at 191.157 kb on - strand, within EX31_RS07005 at 191.165 kb on - strand, within EX31_RS07005 at 191.165 kb on - strand, within EX31_RS07005 at 191.165 kb on - strand, within EX31_RS07005 at 191.258 kb on - strand, within EX31_RS07005 at 191.258 kb on - strand, within EX31_RS07005 at 191.331 kb on + strand, within EX31_RS07005 at 191.471 kb on + strand, within EX31_RS07005 at 191.473 kb on + strand, within EX31_RS07005 at 191.473 kb on + strand, within EX31_RS07005
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 187,913 - -1.1 187,961 - +3.3 187,990 - +0.9 187,990 - -2.2 188,039 + +0.1 188,247 - EX31_RS06995 0.10 -1.3 188,278 + EX31_RS06995 0.15 -0.3 188,279 - EX31_RS06995 0.16 -0.1 188,283 - EX31_RS06995 0.16 -0.3 188,396 + EX31_RS06995 0.35 +0.7 188,396 + EX31_RS06995 0.35 +0.3 188,396 + EX31_RS06995 0.35 +0.7 188,397 - EX31_RS06995 0.35 -0.2 188,397 - EX31_RS06995 0.35 +0.8 188,432 + EX31_RS06995 0.41 -0.2 188,484 + EX31_RS06995 0.49 -0.1 188,484 + EX31_RS06995 0.49 +1.3 188,485 - EX31_RS06995 0.49 -0.0 188,485 - EX31_RS06995 0.49 -0.0 188,509 - EX31_RS06995 0.53 +1.7 188,509 - EX31_RS06995 0.53 +1.1 188,509 - EX31_RS06995 0.53 +0.7 188,589 + EX31_RS06995 0.67 -1.7 188,589 + EX31_RS06995 0.67 +0.4 188,589 + EX31_RS06995 0.67 -0.3 188,589 + EX31_RS06995 0.67 -0.9 188,590 - EX31_RS06995 0.67 -0.3 188,591 + EX31_RS06995 0.67 +0.1 188,591 + EX31_RS06995 0.67 -1.5 188,645 + EX31_RS06995 0.76 -0.5 188,645 + EX31_RS06995 0.76 -2.7 188,646 - EX31_RS06995 0.76 +0.2 188,662 + EX31_RS06995 0.78 -0.1 188,662 + EX31_RS06995 0.78 +2.0 188,663 - EX31_RS06995 0.79 -0.3 188,676 + EX31_RS06995 0.81 +0.7 188,680 + EX31_RS06995 0.81 +0.5 188,680 + EX31_RS06995 0.81 +0.2 188,849 + -0.8 189,098 + EX31_RS07000 0.15 +0.8 189,098 + EX31_RS07000 0.15 +1.1 189,098 + EX31_RS07000 0.15 -0.9 189,099 - EX31_RS07000 0.15 +0.1 189,099 - EX31_RS07000 0.15 -0.8 189,099 - EX31_RS07000 0.15 -0.4 189,099 - EX31_RS07000 0.15 +0.5 189,099 - EX31_RS07000 0.15 +0.1 189,099 - EX31_RS07000 0.15 +0.1 189,101 - EX31_RS07000 0.16 +0.2 189,209 - EX31_RS07000 0.22 -1.0 189,222 + EX31_RS07000 0.23 +0.5 189,223 - EX31_RS07000 0.23 +0.3 189,362 - EX31_RS07000 0.31 -0.9 189,463 + EX31_RS07000 0.37 -1.5 189,507 + EX31_RS07000 0.39 -0.3 189,508 - EX31_RS07000 0.39 +0.4 189,743 - EX31_RS07000 0.53 -0.0 189,817 - EX31_RS07000 0.57 +0.3 190,259 - EX31_RS07000 0.82 -1.8 190,337 - EX31_RS07000 0.87 -0.9 190,374 - EX31_RS07000 0.89 -0.7 190,457 + +0.7 190,799 + EX31_RS07005 0.22 +0.3 190,799 + EX31_RS07005 0.22 -0.7 190,799 + EX31_RS07005 0.22 +1.3 190,800 - EX31_RS07005 0.22 -0.7 190,800 - EX31_RS07005 0.22 +0.1 190,800 - EX31_RS07005 0.22 +0.2 190,890 - EX31_RS07005 0.31 -0.5 190,924 + EX31_RS07005 0.34 -0.3 191,135 + EX31_RS07005 0.55 +1.1 191,156 + EX31_RS07005 0.58 -0.9 191,157 - EX31_RS07005 0.58 +2.1 191,165 - EX31_RS07005 0.58 -0.4 191,165 - EX31_RS07005 0.58 +0.2 191,165 - EX31_RS07005 0.58 -1.8 191,258 - EX31_RS07005 0.68 -0.3 191,258 - EX31_RS07005 0.68 +0.9 191,331 + EX31_RS07005 0.75 -0.8 191,471 + EX31_RS07005 0.89 +0.1 191,473 + EX31_RS07005 0.89 +0.9 191,473 + EX31_RS07005 0.89 -0.7
Or see this region's nucleotide sequence