Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06400 and EX31_RS06405 are separated by 10 nucleotides EX31_RS06405 and EX31_RS06410 are separated by 94 nucleotides
EX31_RS06400: EX31_RS06400 - chondroitin lyase, at 44,326 to 46,464
_RS06400
EX31_RS06405: EX31_RS06405 - SDR family oxidoreductase, at 46,475 to 47,251
_RS06405
EX31_RS06410: EX31_RS06410 - FadR family transcriptional regulator, at 47,346 to 48,056
_RS06410
Position (kb) 46
47
48 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 45.623 kb on - strand, within EX31_RS06400 at 45.623 kb on - strand, within EX31_RS06400 at 45.639 kb on + strand, within EX31_RS06400 at 45.644 kb on - strand, within EX31_RS06400 at 45.644 kb on - strand, within EX31_RS06400 at 45.644 kb on - strand, within EX31_RS06400 at 45.769 kb on + strand, within EX31_RS06400 at 45.792 kb on + strand, within EX31_RS06400 at 45.792 kb on + strand, within EX31_RS06400 at 45.793 kb on - strand, within EX31_RS06400 at 45.793 kb on - strand, within EX31_RS06400 at 45.799 kb on + strand, within EX31_RS06400 at 45.800 kb on - strand, within EX31_RS06400 at 45.802 kb on + strand, within EX31_RS06400 at 46.016 kb on + strand, within EX31_RS06400 at 46.087 kb on + strand, within EX31_RS06400 at 46.164 kb on + strand, within EX31_RS06400 at 46.187 kb on + strand, within EX31_RS06400 at 46.246 kb on + strand, within EX31_RS06400 at 46.346 kb on - strand at 46.462 kb on + strand at 46.474 kb on + strand at 46.528 kb on + strand at 46.528 kb on + strand at 46.714 kb on - strand, within EX31_RS06405 at 46.737 kb on + strand, within EX31_RS06405 at 46.737 kb on + strand, within EX31_RS06405 at 46.738 kb on - strand, within EX31_RS06405 at 47.169 kb on - strand, within EX31_RS06405 at 47.201 kb on + strand at 47.202 kb on - strand at 47.308 kb on + strand at 47.308 kb on + strand at 47.308 kb on + strand at 47.309 kb on - strand at 47.309 kb on - strand at 47.309 kb on - strand at 47.309 kb on - strand at 47.309 kb on - strand at 47.309 kb on - strand at 47.654 kb on + strand, within EX31_RS06410 at 47.658 kb on + strand, within EX31_RS06410 at 47.659 kb on - strand, within EX31_RS06410 at 47.954 kb on + strand, within EX31_RS06410 at 47.954 kb on + strand, within EX31_RS06410 at 47.960 kb on + strand, within EX31_RS06410 at 47.960 kb on + strand, within EX31_RS06410 at 47.960 kb on + strand, within EX31_RS06410 at 47.961 kb on - strand, within EX31_RS06410 at 47.965 kb on - strand, within EX31_RS06410 at 48.032 kb on + strand at 48.054 kb on + strand at 48.054 kb on + strand at 48.054 kb on + strand at 48.055 kb on - strand at 48.118 kb on - strand at 48.201 kb on - strand at 48.205 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 45,623 - EX31_RS06400 0.61 +0.1 45,623 - EX31_RS06400 0.61 -0.4 45,639 + EX31_RS06400 0.61 -0.5 45,644 - EX31_RS06400 0.62 -0.4 45,644 - EX31_RS06400 0.62 +0.6 45,644 - EX31_RS06400 0.62 +1.7 45,769 + EX31_RS06400 0.67 +0.3 45,792 + EX31_RS06400 0.69 -1.1 45,792 + EX31_RS06400 0.69 -0.5 45,793 - EX31_RS06400 0.69 -0.7 45,793 - EX31_RS06400 0.69 +0.7 45,799 + EX31_RS06400 0.69 -0.6 45,800 - EX31_RS06400 0.69 -0.3 45,802 + EX31_RS06400 0.69 +0.9 46,016 + EX31_RS06400 0.79 -0.3 46,087 + EX31_RS06400 0.82 -0.4 46,164 + EX31_RS06400 0.86 +0.5 46,187 + EX31_RS06400 0.87 +0.7 46,246 + EX31_RS06400 0.90 +0.9 46,346 - -0.3 46,462 + -0.8 46,474 + +1.7 46,528 + -0.9 46,528 + +0.1 46,714 - EX31_RS06405 0.31 -1.7 46,737 + EX31_RS06405 0.34 -0.1 46,737 + EX31_RS06405 0.34 -0.3 46,738 - EX31_RS06405 0.34 -0.1 47,169 - EX31_RS06405 0.89 -2.8 47,201 + +0.8 47,202 - +0.2 47,308 + -0.8 47,308 + +0.1 47,308 + -0.1 47,309 - +0.2 47,309 - -0.7 47,309 - +0.8 47,309 - -1.3 47,309 - +0.3 47,309 - +0.6 47,654 + EX31_RS06410 0.43 -0.1 47,658 + EX31_RS06410 0.44 +1.0 47,659 - EX31_RS06410 0.44 -0.5 47,954 + EX31_RS06410 0.86 +0.6 47,954 + EX31_RS06410 0.86 -0.4 47,960 + EX31_RS06410 0.86 +0.1 47,960 + EX31_RS06410 0.86 +0.4 47,960 + EX31_RS06410 0.86 +0.4 47,961 - EX31_RS06410 0.86 +0.3 47,965 - EX31_RS06410 0.87 +1.7 48,032 + +0.5 48,054 + -0.4 48,054 + -0.8 48,054 + -0.9 48,055 - +2.9 48,118 - -1.6 48,201 - -0.6 48,205 - +1.7
Or see this region's nucleotide sequence