Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS04610 and EX31_RS04615 are separated by 3 nucleotides EX31_RS04615 and EX31_RS04620 are separated by 362 nucleotides
EX31_RS04610: EX31_RS04610 - phosphoribulokinase, at 34,715 to 35,584
_RS04610
EX31_RS04615: EX31_RS04615 - OsmC family protein, at 35,588 to 35,995
_RS04615
EX31_RS04620: EX31_RS04620 - cAMP-activated global transcriptional regulator CRP, at 36,358 to 36,990
_RS04620
Position (kb) 35
36 Strain fitness (log2 ratio) -2
-1
0
1
2 at 34.605 kb on - strand at 34.605 kb on - strand at 34.608 kb on + strand at 34.608 kb on + strand at 34.609 kb on - strand at 34.609 kb on - strand at 34.609 kb on - strand at 34.609 kb on - strand at 34.609 kb on - strand at 34.609 kb on - strand at 34.614 kb on - strand at 34.619 kb on + strand at 34.619 kb on + strand at 34.619 kb on + strand at 34.619 kb on + strand at 34.619 kb on + strand at 34.620 kb on - strand at 34.620 kb on - strand at 34.620 kb on - strand at 34.625 kb on + strand at 34.625 kb on + strand at 34.626 kb on - strand at 34.626 kb on - strand at 34.630 kb on - strand at 34.714 kb on + strand at 34.714 kb on + strand at 34.715 kb on - strand at 34.735 kb on + strand at 34.736 kb on - strand at 34.909 kb on + strand, within EX31_RS04610 at 34.909 kb on + strand, within EX31_RS04610 at 34.909 kb on + strand, within EX31_RS04610 at 34.909 kb on + strand, within EX31_RS04610 at 34.910 kb on - strand, within EX31_RS04610 at 34.910 kb on - strand, within EX31_RS04610 at 34.910 kb on - strand, within EX31_RS04610 at 34.916 kb on + strand, within EX31_RS04610 at 34.916 kb on + strand, within EX31_RS04610 at 34.917 kb on - strand, within EX31_RS04610 at 34.917 kb on - strand, within EX31_RS04610 at 34.976 kb on - strand, within EX31_RS04610 at 34.999 kb on + strand, within EX31_RS04610 at 34.999 kb on + strand, within EX31_RS04610 at 35.000 kb on - strand, within EX31_RS04610 at 35.000 kb on - strand, within EX31_RS04610 at 35.000 kb on - strand, within EX31_RS04610 at 35.000 kb on - strand, within EX31_RS04610 at 35.000 kb on - strand, within EX31_RS04610 at 35.000 kb on - strand, within EX31_RS04610 at 35.004 kb on - strand, within EX31_RS04610 at 35.011 kb on + strand, within EX31_RS04610 at 35.011 kb on + strand, within EX31_RS04610 at 35.011 kb on + strand, within EX31_RS04610 at 35.011 kb on + strand, within EX31_RS04610 at 35.012 kb on - strand, within EX31_RS04610 at 35.012 kb on - strand, within EX31_RS04610 at 35.012 kb on - strand, within EX31_RS04610 at 35.012 kb on - strand, within EX31_RS04610 at 35.012 kb on - strand, within EX31_RS04610 at 35.012 kb on - strand, within EX31_RS04610 at 35.012 kb on - strand, within EX31_RS04610 at 35.012 kb on - strand, within EX31_RS04610 at 35.012 kb on - strand, within EX31_RS04610 at 35.015 kb on + strand, within EX31_RS04610 at 35.015 kb on + strand, within EX31_RS04610 at 35.016 kb on - strand, within EX31_RS04610 at 35.033 kb on + strand, within EX31_RS04610 at 35.033 kb on + strand, within EX31_RS04610 at 35.034 kb on - strand, within EX31_RS04610 at 35.034 kb on - strand, within EX31_RS04610 at 35.099 kb on + strand, within EX31_RS04610 at 35.099 kb on + strand, within EX31_RS04610 at 35.100 kb on - strand, within EX31_RS04610 at 35.100 kb on - strand, within EX31_RS04610 at 35.110 kb on - strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.282 kb on + strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.283 kb on - strand, within EX31_RS04610 at 35.284 kb on + strand, within EX31_RS04610 at 35.284 kb on + strand, within EX31_RS04610 at 35.284 kb on + strand, within EX31_RS04610 at 35.285 kb on - strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.291 kb on + strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.292 kb on - strand, within EX31_RS04610 at 35.473 kb on + strand, within EX31_RS04610 at 35.661 kb on + strand, within EX31_RS04615 at 35.662 kb on - strand, within EX31_RS04615 at 35.701 kb on + strand, within EX31_RS04615 at 35.701 kb on + strand, within EX31_RS04615 at 35.728 kb on + strand, within EX31_RS04615 at 35.815 kb on + strand, within EX31_RS04615 at 35.815 kb on + strand, within EX31_RS04615 at 35.854 kb on + strand, within EX31_RS04615 at 35.854 kb on + strand, within EX31_RS04615 at 35.854 kb on + strand, within EX31_RS04615 at 35.876 kb on + strand, within EX31_RS04615 at 35.877 kb on - strand, within EX31_RS04615 at 35.877 kb on - strand, within EX31_RS04615 at 35.902 kb on + strand, within EX31_RS04615 at 35.903 kb on - strand, within EX31_RS04615 at 35.954 kb on + strand, within EX31_RS04615 at 35.955 kb on - strand at 36.000 kb on + strand at 36.000 kb on + strand at 36.000 kb on + strand at 36.000 kb on + strand at 36.001 kb on - strand at 36.001 kb on - strand at 36.001 kb on - strand at 36.016 kb on - strand at 36.016 kb on - strand at 36.016 kb on - strand at 36.055 kb on - strand at 36.069 kb on + strand at 36.070 kb on - strand at 36.070 kb on - strand at 36.147 kb on + strand at 36.147 kb on + strand at 36.147 kb on + strand at 36.147 kb on + strand at 36.148 kb on - strand at 36.148 kb on - strand at 36.148 kb on - strand at 36.148 kb on - strand at 36.148 kb on - strand at 36.184 kb on + strand at 36.184 kb on + strand at 36.184 kb on + strand at 36.185 kb on - strand at 36.185 kb on - strand at 36.214 kb on + strand at 36.215 kb on - strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.263 kb on + strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.264 kb on - strand at 36.265 kb on + strand at 36.265 kb on + strand at 36.299 kb on + strand at 36.427 kb on + strand, within EX31_RS04620 at 36.989 kb on - strand at 36.989 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 34,605 - +0.3 34,605 - -0.4 34,608 + -0.4 34,608 + +0.0 34,609 - -0.6 34,609 - +1.0 34,609 - +0.7 34,609 - -0.0 34,609 - +1.4 34,609 - -0.7 34,614 - +0.3 34,619 + +0.3 34,619 + -0.4 34,619 + -0.2 34,619 + +1.3 34,619 + -0.4 34,620 - -0.4 34,620 - -0.5 34,620 - -0.8 34,625 + +1.6 34,625 + +0.6 34,626 - +0.4 34,626 - +0.4 34,630 - +1.2 34,714 + -1.2 34,714 + -0.2 34,715 - +0.1 34,735 + -1.2 34,736 - -2.4 34,909 + EX31_RS04610 0.22 -0.8 34,909 + EX31_RS04610 0.22 -0.6 34,909 + EX31_RS04610 0.22 -0.1 34,909 + EX31_RS04610 0.22 +0.1 34,910 - EX31_RS04610 0.22 -1.9 34,910 - EX31_RS04610 0.22 -0.6 34,910 - EX31_RS04610 0.22 +0.1 34,916 + EX31_RS04610 0.23 -0.8 34,916 + EX31_RS04610 0.23 -1.9 34,917 - EX31_RS04610 0.23 -0.8 34,917 - EX31_RS04610 0.23 +0.1 34,976 - EX31_RS04610 0.30 -0.6 34,999 + EX31_RS04610 0.33 +1.4 34,999 + EX31_RS04610 0.33 -0.8 35,000 - EX31_RS04610 0.33 -0.5 35,000 - EX31_RS04610 0.33 +1.3 35,000 - EX31_RS04610 0.33 -0.3 35,000 - EX31_RS04610 0.33 -0.6 35,000 - EX31_RS04610 0.33 -1.6 35,000 - EX31_RS04610 0.33 +0.7 35,004 - EX31_RS04610 0.33 +1.1 35,011 + EX31_RS04610 0.34 -1.2 35,011 + EX31_RS04610 0.34 +0.1 35,011 + EX31_RS04610 0.34 +0.8 35,011 + EX31_RS04610 0.34 -0.1 35,012 - EX31_RS04610 0.34 -0.9 35,012 - EX31_RS04610 0.34 +0.7 35,012 - EX31_RS04610 0.34 +0.6 35,012 - EX31_RS04610 0.34 +0.5 35,012 - EX31_RS04610 0.34 -0.7 35,012 - EX31_RS04610 0.34 -0.5 35,012 - EX31_RS04610 0.34 -1.5 35,012 - EX31_RS04610 0.34 +0.7 35,012 - EX31_RS04610 0.34 +0.8 35,015 + EX31_RS04610 0.34 +1.5 35,015 + EX31_RS04610 0.34 -0.7 35,016 - EX31_RS04610 0.35 -0.6 35,033 + EX31_RS04610 0.37 -0.3 35,033 + EX31_RS04610 0.37 -0.6 35,034 - EX31_RS04610 0.37 +0.5 35,034 - EX31_RS04610 0.37 -0.3 35,099 + EX31_RS04610 0.44 -0.2 35,099 + EX31_RS04610 0.44 -0.6 35,100 - EX31_RS04610 0.44 +2.5 35,100 - EX31_RS04610 0.44 +0.4 35,110 - EX31_RS04610 0.45 +0.6 35,282 + EX31_RS04610 0.65 -0.4 35,282 + EX31_RS04610 0.65 -0.2 35,282 + EX31_RS04610 0.65 -0.8 35,282 + EX31_RS04610 0.65 +0.1 35,282 + EX31_RS04610 0.65 -0.1 35,282 + EX31_RS04610 0.65 -0.3 35,282 + EX31_RS04610 0.65 -0.1 35,282 + EX31_RS04610 0.65 -0.5 35,282 + EX31_RS04610 0.65 +0.8 35,282 + EX31_RS04610 0.65 -0.2 35,282 + EX31_RS04610 0.65 -0.4 35,282 + EX31_RS04610 0.65 -0.8 35,282 + EX31_RS04610 0.65 -1.8 35,282 + EX31_RS04610 0.65 +1.1 35,282 + EX31_RS04610 0.65 +1.0 35,282 + EX31_RS04610 0.65 +0.6 35,282 + EX31_RS04610 0.65 -0.6 35,282 + EX31_RS04610 0.65 +0.6 35,282 + EX31_RS04610 0.65 +0.1 35,282 + EX31_RS04610 0.65 +0.9 35,283 - EX31_RS04610 0.65 +0.8 35,283 - EX31_RS04610 0.65 +0.3 35,283 - EX31_RS04610 0.65 +0.1 35,283 - EX31_RS04610 0.65 -0.6 35,283 - EX31_RS04610 0.65 -0.2 35,283 - EX31_RS04610 0.65 +0.7 35,283 - EX31_RS04610 0.65 +1.7 35,283 - EX31_RS04610 0.65 +1.0 35,283 - EX31_RS04610 0.65 +1.5 35,283 - EX31_RS04610 0.65 -1.0 35,283 - EX31_RS04610 0.65 +0.1 35,283 - EX31_RS04610 0.65 +0.7 35,283 - EX31_RS04610 0.65 +0.4 35,284 + EX31_RS04610 0.65 +0.6 35,284 + EX31_RS04610 0.65 -0.6 35,284 + EX31_RS04610 0.65 +0.1 35,285 - EX31_RS04610 0.66 -0.3 35,291 + EX31_RS04610 0.66 -0.2 35,291 + EX31_RS04610 0.66 +0.8 35,291 + EX31_RS04610 0.66 +1.0 35,291 + EX31_RS04610 0.66 +0.1 35,291 + EX31_RS04610 0.66 +0.6 35,291 + EX31_RS04610 0.66 +0.5 35,291 + EX31_RS04610 0.66 +0.6 35,291 + EX31_RS04610 0.66 -0.7 35,291 + EX31_RS04610 0.66 -0.6 35,291 + EX31_RS04610 0.66 +0.9 35,291 + EX31_RS04610 0.66 -1.8 35,291 + EX31_RS04610 0.66 +0.1 35,291 + EX31_RS04610 0.66 -0.7 35,291 + EX31_RS04610 0.66 +0.8 35,291 + EX31_RS04610 0.66 -1.1 35,291 + EX31_RS04610 0.66 -0.2 35,291 + EX31_RS04610 0.66 +0.4 35,291 + EX31_RS04610 0.66 +0.1 35,291 + EX31_RS04610 0.66 -0.8 35,291 + EX31_RS04610 0.66 -0.1 35,291 + EX31_RS04610 0.66 +0.4 35,292 - EX31_RS04610 0.66 -0.8 35,292 - EX31_RS04610 0.66 +0.2 35,292 - EX31_RS04610 0.66 +0.6 35,292 - EX31_RS04610 0.66 +0.7 35,292 - EX31_RS04610 0.66 -1.7 35,292 - EX31_RS04610 0.66 +1.3 35,292 - EX31_RS04610 0.66 -0.3 35,292 - EX31_RS04610 0.66 +0.4 35,292 - EX31_RS04610 0.66 -0.3 35,292 - EX31_RS04610 0.66 +0.6 35,292 - EX31_RS04610 0.66 -0.3 35,292 - EX31_RS04610 0.66 +0.1 35,473 + EX31_RS04610 0.87 -0.4 35,661 + EX31_RS04615 0.18 -0.7 35,662 - EX31_RS04615 0.18 +0.6 35,701 + EX31_RS04615 0.28 -1.6 35,701 + EX31_RS04615 0.28 -0.2 35,728 + EX31_RS04615 0.34 +1.3 35,815 + EX31_RS04615 0.56 +0.2 35,815 + EX31_RS04615 0.56 +0.3 35,854 + EX31_RS04615 0.65 +0.0 35,854 + EX31_RS04615 0.65 +2.2 35,854 + EX31_RS04615 0.65 +0.2 35,876 + EX31_RS04615 0.71 -0.4 35,877 - EX31_RS04615 0.71 +0.2 35,877 - EX31_RS04615 0.71 +0.3 35,902 + EX31_RS04615 0.77 +0.5 35,903 - EX31_RS04615 0.77 +0.0 35,954 + EX31_RS04615 0.90 +0.8 35,955 - +0.2 36,000 + -1.1 36,000 + +0.2 36,000 + +0.2 36,000 + -0.5 36,001 - +1.3 36,001 - -1.1 36,001 - +0.7 36,016 - +0.1 36,016 - +2.7 36,016 - +0.5 36,055 - +0.2 36,069 + +0.0 36,070 - -0.6 36,070 - +0.4 36,147 + +1.6 36,147 + +0.6 36,147 + +0.4 36,147 + +0.6 36,148 - -0.2 36,148 - -1.7 36,148 - +0.5 36,148 - +0.6 36,148 - -0.6 36,184 + +1.4 36,184 + -1.0 36,184 + -0.6 36,185 - +0.8 36,185 - +0.4 36,214 + -0.1 36,215 - -1.0 36,263 + +0.4 36,263 + +0.4 36,263 + -1.5 36,263 + +0.2 36,263 + +0.8 36,263 + +0.9 36,263 + -0.3 36,263 + +0.8 36,263 + +0.0 36,263 + -0.9 36,263 + -0.0 36,263 + +1.1 36,263 + +0.1 36,263 + +0.4 36,263 + +0.4 36,263 + +0.6 36,263 + +1.1 36,263 + +0.4 36,263 + -0.2 36,263 + +0.2 36,263 + +0.4 36,264 - -0.6 36,264 - +1.2 36,264 - +0.4 36,264 - +0.5 36,264 - -0.5 36,264 - -1.1 36,264 - -0.4 36,264 - -1.2 36,264 - -0.1 36,264 - -1.0 36,264 - +0.0 36,264 - +0.5 36,264 - -0.2 36,265 + -0.7 36,265 + +0.6 36,299 + -0.9 36,427 + EX31_RS04620 0.11 +0.9 36,989 - -1.2 36,989 - -0.5
Or see this region's nucleotide sequence