Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS04385 and EX31_RS04390 are separated by 115 nucleotides EX31_RS04390 and EX31_RS04395 overlap by 8 nucleotides
EX31_RS04385: EX31_RS04385 - leucyl aminopeptidase, at 406,184 to 407,695
_RS04385
EX31_RS04390: EX31_RS04390 - DNA polymerase III subunit chi, at 407,811 to 408,296
_RS04390
EX31_RS04395: EX31_RS04395 - valine--tRNA ligase, at 408,289 to 411,144
_RS04395
Position (kb) 407
408
409 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 406.889 kb on + strand, within EX31_RS04385 at 406.889 kb on + strand, within EX31_RS04385 at 406.890 kb on - strand, within EX31_RS04385 at 406.890 kb on - strand, within EX31_RS04385 at 406.890 kb on - strand, within EX31_RS04385 at 406.943 kb on + strand, within EX31_RS04385 at 406.943 kb on + strand, within EX31_RS04385 at 406.943 kb on + strand, within EX31_RS04385 at 406.944 kb on - strand, within EX31_RS04385 at 406.944 kb on - strand, within EX31_RS04385 at 406.944 kb on - strand, within EX31_RS04385 at 406.945 kb on + strand, within EX31_RS04385 at 406.946 kb on - strand, within EX31_RS04385 at 406.969 kb on + strand, within EX31_RS04385 at 406.975 kb on + strand, within EX31_RS04385 at 406.975 kb on + strand, within EX31_RS04385 at 407.017 kb on + strand, within EX31_RS04385 at 407.017 kb on + strand, within EX31_RS04385 at 407.018 kb on - strand, within EX31_RS04385 at 407.018 kb on - strand, within EX31_RS04385 at 407.057 kb on + strand, within EX31_RS04385 at 407.057 kb on + strand, within EX31_RS04385 at 407.058 kb on - strand, within EX31_RS04385 at 407.233 kb on + strand, within EX31_RS04385 at 407.276 kb on + strand, within EX31_RS04385 at 407.276 kb on + strand, within EX31_RS04385 at 407.276 kb on + strand, within EX31_RS04385 at 407.276 kb on + strand, within EX31_RS04385 at 407.276 kb on + strand, within EX31_RS04385 at 407.276 kb on + strand, within EX31_RS04385 at 407.276 kb on + strand, within EX31_RS04385 at 407.277 kb on - strand, within EX31_RS04385 at 407.277 kb on - strand, within EX31_RS04385 at 407.277 kb on - strand, within EX31_RS04385 at 407.277 kb on - strand, within EX31_RS04385 at 407.277 kb on - strand, within EX31_RS04385 at 407.277 kb on - strand, within EX31_RS04385 at 407.277 kb on - strand, within EX31_RS04385 at 407.277 kb on - strand, within EX31_RS04385 at 407.277 kb on - strand, within EX31_RS04385 at 407.459 kb on + strand, within EX31_RS04385 at 407.480 kb on - strand, within EX31_RS04385 at 407.564 kb on + strand at 407.564 kb on + strand at 407.564 kb on + strand at 407.565 kb on - strand at 407.565 kb on - strand at 407.565 kb on - strand at 407.565 kb on - strand at 407.565 kb on - strand at 407.565 kb on - strand at 407.705 kb on + strand at 407.705 kb on + strand at 407.705 kb on + strand at 407.709 kb on + strand at 407.709 kb on + strand at 407.709 kb on + strand at 407.709 kb on + strand at 407.709 kb on + strand at 407.709 kb on + strand at 407.709 kb on + strand at 407.710 kb on - strand at 407.710 kb on - strand at 407.710 kb on - strand at 407.710 kb on - strand at 407.714 kb on + strand at 407.714 kb on + strand at 407.714 kb on + strand at 407.714 kb on + strand at 407.714 kb on + strand at 407.715 kb on - strand at 407.715 kb on - strand at 407.715 kb on - strand at 407.715 kb on - strand at 407.715 kb on - strand at 407.715 kb on - strand at 407.715 kb on - strand at 407.716 kb on + strand at 407.716 kb on + strand at 407.716 kb on + strand at 407.716 kb on + strand at 407.716 kb on + strand at 407.716 kb on + strand at 407.716 kb on + strand at 407.716 kb on + strand at 407.716 kb on + strand at 407.716 kb on + strand at 407.716 kb on + strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.717 kb on - strand at 407.726 kb on + strand at 407.726 kb on + strand at 408.055 kb on + strand, within EX31_RS04390 at 408.055 kb on + strand, within EX31_RS04390 at 408.055 kb on + strand, within EX31_RS04390 at 408.055 kb on + strand, within EX31_RS04390 at 408.055 kb on + strand, within EX31_RS04390 at 408.179 kb on + strand, within EX31_RS04390
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 406,889 + EX31_RS04385 0.47 -1.0 406,889 + EX31_RS04385 0.47 -0.2 406,890 - EX31_RS04385 0.47 -0.6 406,890 - EX31_RS04385 0.47 -0.5 406,890 - EX31_RS04385 0.47 +1.3 406,943 + EX31_RS04385 0.50 -0.6 406,943 + EX31_RS04385 0.50 +0.5 406,943 + EX31_RS04385 0.50 -0.3 406,944 - EX31_RS04385 0.50 +0.1 406,944 - EX31_RS04385 0.50 +1.0 406,944 - EX31_RS04385 0.50 -0.0 406,945 + EX31_RS04385 0.50 +0.3 406,946 - EX31_RS04385 0.50 +0.6 406,969 + EX31_RS04385 0.52 +0.0 406,975 + EX31_RS04385 0.52 -0.9 406,975 + EX31_RS04385 0.52 +1.3 407,017 + EX31_RS04385 0.55 -0.8 407,017 + EX31_RS04385 0.55 -0.2 407,018 - EX31_RS04385 0.55 +1.0 407,018 - EX31_RS04385 0.55 -0.7 407,057 + EX31_RS04385 0.58 +0.4 407,057 + EX31_RS04385 0.58 -0.2 407,058 - EX31_RS04385 0.58 -0.5 407,233 + EX31_RS04385 0.69 -0.4 407,276 + EX31_RS04385 0.72 +0.7 407,276 + EX31_RS04385 0.72 -0.1 407,276 + EX31_RS04385 0.72 -1.5 407,276 + EX31_RS04385 0.72 -0.1 407,276 + EX31_RS04385 0.72 +1.0 407,276 + EX31_RS04385 0.72 +3.0 407,276 + EX31_RS04385 0.72 -0.8 407,277 - EX31_RS04385 0.72 +0.4 407,277 - EX31_RS04385 0.72 -2.0 407,277 - EX31_RS04385 0.72 -0.4 407,277 - EX31_RS04385 0.72 -1.2 407,277 - EX31_RS04385 0.72 +0.6 407,277 - EX31_RS04385 0.72 -0.3 407,277 - EX31_RS04385 0.72 -0.1 407,277 - EX31_RS04385 0.72 -0.5 407,277 - EX31_RS04385 0.72 +0.4 407,459 + EX31_RS04385 0.84 +0.1 407,480 - EX31_RS04385 0.86 -0.9 407,564 + -0.3 407,564 + -0.9 407,564 + -0.1 407,565 - -0.4 407,565 - +0.7 407,565 - +0.5 407,565 - -1.9 407,565 - -0.9 407,565 - -0.0 407,705 + +0.0 407,705 + -0.4 407,705 + -0.3 407,709 + +0.7 407,709 + +0.5 407,709 + +0.7 407,709 + +0.7 407,709 + +0.5 407,709 + +0.9 407,709 + -0.4 407,710 - +0.3 407,710 - +0.9 407,710 - -0.2 407,710 - -0.5 407,714 + +0.5 407,714 + +0.0 407,714 + +0.5 407,714 + +1.6 407,714 + -0.8 407,715 - -0.0 407,715 - -0.3 407,715 - -0.9 407,715 - -0.9 407,715 - -1.1 407,715 - +1.2 407,715 - +0.5 407,716 + -1.1 407,716 + -0.2 407,716 + -0.5 407,716 + -0.6 407,716 + -0.4 407,716 + +1.0 407,716 + -0.3 407,716 + -0.2 407,716 + +0.1 407,716 + +0.2 407,716 + -1.2 407,717 - +0.7 407,717 - -0.4 407,717 - -1.2 407,717 - +0.0 407,717 - +1.7 407,717 - -0.8 407,717 - +2.0 407,717 - -1.5 407,717 - -0.6 407,717 - -0.3 407,717 - +0.5 407,717 - +1.5 407,717 - -2.1 407,726 + +0.1 407,726 + -0.6 408,055 + EX31_RS04390 0.50 -0.2 408,055 + EX31_RS04390 0.50 -0.5 408,055 + EX31_RS04390 0.50 -2.8 408,055 + EX31_RS04390 0.50 -2.1 408,055 + EX31_RS04390 0.50 -1.3 408,179 + EX31_RS04390 0.76 -1.0
Or see this region's nucleotide sequence