Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS04030 and EX31_RS04035 are separated by 20 nucleotides EX31_RS04035 and EX31_RS04040 are separated by 234 nucleotides
EX31_RS04030: EX31_RS04030 - DNA repair protein RadA, at 327,765 to 329,147
_RS04030
EX31_RS04035: EX31_RS04035 - phosphoserine phosphatase, at 329,168 to 330,133
_RS04035
EX31_RS04040: EX31_RS04040 - YtjB family periplasmic protein, at 330,368 to 331,156
_RS04040
Position (kb) 329
330
331 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 328.342 kb on - strand, within EX31_RS04030 at 328.342 kb on - strand, within EX31_RS04030 at 328.342 kb on - strand, within EX31_RS04030 at 328.439 kb on + strand, within EX31_RS04030 at 328.439 kb on + strand, within EX31_RS04030 at 328.440 kb on - strand, within EX31_RS04030 at 328.440 kb on - strand, within EX31_RS04030 at 328.481 kb on + strand, within EX31_RS04030 at 328.482 kb on - strand, within EX31_RS04030 at 328.540 kb on + strand, within EX31_RS04030 at 328.541 kb on - strand, within EX31_RS04030 at 328.691 kb on + strand, within EX31_RS04030 at 328.691 kb on + strand, within EX31_RS04030 at 328.691 kb on + strand, within EX31_RS04030 at 328.691 kb on + strand, within EX31_RS04030 at 328.691 kb on + strand, within EX31_RS04030 at 328.691 kb on + strand, within EX31_RS04030 at 328.691 kb on + strand, within EX31_RS04030 at 328.691 kb on + strand, within EX31_RS04030 at 328.691 kb on + strand, within EX31_RS04030 at 328.691 kb on + strand, within EX31_RS04030 at 328.691 kb on + strand, within EX31_RS04030 at 328.692 kb on - strand, within EX31_RS04030 at 328.692 kb on - strand, within EX31_RS04030 at 328.692 kb on - strand, within EX31_RS04030 at 328.692 kb on - strand, within EX31_RS04030 at 328.692 kb on - strand, within EX31_RS04030 at 328.692 kb on - strand, within EX31_RS04030 at 328.692 kb on - strand, within EX31_RS04030 at 328.692 kb on - strand, within EX31_RS04030 at 328.701 kb on - strand, within EX31_RS04030 at 328.701 kb on - strand, within EX31_RS04030 at 328.701 kb on - strand, within EX31_RS04030 at 328.768 kb on + strand, within EX31_RS04030 at 328.768 kb on + strand, within EX31_RS04030 at 328.768 kb on + strand, within EX31_RS04030 at 328.769 kb on - strand, within EX31_RS04030 at 328.769 kb on - strand, within EX31_RS04030 at 328.769 kb on - strand, within EX31_RS04030 at 328.820 kb on + strand, within EX31_RS04030 at 328.821 kb on - strand, within EX31_RS04030 at 328.821 kb on - strand, within EX31_RS04030 at 328.821 kb on - strand, within EX31_RS04030 at 328.872 kb on + strand, within EX31_RS04030 at 328.873 kb on - strand, within EX31_RS04030 at 328.884 kb on + strand, within EX31_RS04030 at 328.885 kb on - strand, within EX31_RS04030 at 328.885 kb on - strand, within EX31_RS04030 at 328.885 kb on - strand, within EX31_RS04030 at 328.887 kb on + strand, within EX31_RS04030 at 328.887 kb on + strand, within EX31_RS04030 at 328.887 kb on + strand, within EX31_RS04030 at 328.887 kb on + strand, within EX31_RS04030 at 328.887 kb on + strand, within EX31_RS04030 at 328.888 kb on - strand, within EX31_RS04030 at 328.888 kb on - strand, within EX31_RS04030 at 328.888 kb on - strand, within EX31_RS04030 at 328.888 kb on - strand, within EX31_RS04030 at 328.888 kb on - strand, within EX31_RS04030 at 328.888 kb on - strand, within EX31_RS04030 at 328.888 kb on - strand, within EX31_RS04030 at 328.905 kb on - strand, within EX31_RS04030 at 328.944 kb on - strand, within EX31_RS04030 at 329.006 kb on + strand, within EX31_RS04030 at 329.073 kb on - strand at 329.073 kb on - strand at 329.073 kb on - strand at 329.073 kb on - strand at 329.073 kb on - strand at 329.073 kb on - strand at 329.073 kb on - strand at 329.095 kb on + strand at 329.152 kb on + strand at 329.152 kb on + strand at 329.152 kb on + strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.153 kb on - strand at 329.162 kb on + strand at 329.163 kb on - strand at 329.163 kb on - strand at 329.163 kb on - strand at 329.163 kb on - strand at 329.163 kb on - strand at 329.170 kb on - strand at 329.186 kb on + strand at 329.187 kb on - strand at 329.187 kb on - strand at 329.187 kb on - strand at 329.228 kb on + strand at 329.228 kb on + strand at 329.228 kb on + strand at 329.245 kb on - strand at 329.266 kb on - strand, within EX31_RS04035 at 329.266 kb on - strand, within EX31_RS04035 at 329.266 kb on - strand, within EX31_RS04035 at 329.376 kb on + strand, within EX31_RS04035 at 329.376 kb on + strand, within EX31_RS04035 at 329.377 kb on + strand, within EX31_RS04035 at 329.378 kb on - strand, within EX31_RS04035 at 329.378 kb on - strand, within EX31_RS04035 at 329.386 kb on + strand, within EX31_RS04035 at 329.386 kb on + strand, within EX31_RS04035 at 329.386 kb on + strand, within EX31_RS04035 at 329.386 kb on + strand, within EX31_RS04035 at 329.386 kb on + strand, within EX31_RS04035 at 329.386 kb on + strand, within EX31_RS04035 at 329.386 kb on + strand, within EX31_RS04035 at 329.388 kb on + strand, within EX31_RS04035 at 329.388 kb on + strand, within EX31_RS04035 at 329.388 kb on + strand, within EX31_RS04035 at 329.388 kb on + strand, within EX31_RS04035 at 329.389 kb on - strand, within EX31_RS04035 at 329.389 kb on - strand, within EX31_RS04035 at 329.389 kb on - strand, within EX31_RS04035 at 329.389 kb on - strand, within EX31_RS04035 at 329.389 kb on - strand, within EX31_RS04035 at 329.389 kb on - strand, within EX31_RS04035 at 329.431 kb on + strand, within EX31_RS04035 at 329.493 kb on + strand, within EX31_RS04035 at 329.503 kb on - strand, within EX31_RS04035 at 329.503 kb on - strand, within EX31_RS04035 at 329.505 kb on + strand, within EX31_RS04035 at 329.505 kb on + strand, within EX31_RS04035 at 329.505 kb on + strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.506 kb on - strand, within EX31_RS04035 at 329.509 kb on + strand, within EX31_RS04035 at 329.509 kb on + strand, within EX31_RS04035 at 329.509 kb on + strand, within EX31_RS04035 at 329.774 kb on - strand, within EX31_RS04035 at 329.870 kb on + strand, within EX31_RS04035 at 329.870 kb on + strand, within EX31_RS04035 at 329.870 kb on + strand, within EX31_RS04035 at 329.871 kb on - strand, within EX31_RS04035 at 329.871 kb on - strand, within EX31_RS04035 at 329.871 kb on - strand, within EX31_RS04035 at 329.871 kb on - strand, within EX31_RS04035 at 329.871 kb on - strand, within EX31_RS04035 at 329.871 kb on - strand, within EX31_RS04035 at 329.871 kb on - strand, within EX31_RS04035 at 329.881 kb on + strand, within EX31_RS04035 at 329.910 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.974 kb on + strand, within EX31_RS04035 at 329.975 kb on - strand, within EX31_RS04035 at 329.975 kb on - strand, within EX31_RS04035 at 329.975 kb on - strand, within EX31_RS04035 at 329.975 kb on - strand, within EX31_RS04035 at 329.977 kb on + strand, within EX31_RS04035 at 329.977 kb on + strand, within EX31_RS04035 at 329.977 kb on + strand, within EX31_RS04035 at 329.978 kb on - strand, within EX31_RS04035 at 329.978 kb on - strand, within EX31_RS04035 at 330.114 kb on + strand at 330.114 kb on + strand at 330.114 kb on + strand at 330.115 kb on - strand at 330.115 kb on - strand at 330.115 kb on - strand at 330.115 kb on - strand at 330.161 kb on + strand at 330.174 kb on + strand at 330.184 kb on + strand at 330.185 kb on - strand at 330.185 kb on - strand at 330.259 kb on - strand at 330.260 kb on + strand at 330.260 kb on + strand at 330.261 kb on - strand at 330.261 kb on - strand at 330.310 kb on + strand at 330.310 kb on + strand at 330.310 kb on + strand at 330.310 kb on + strand at 330.310 kb on + strand at 330.310 kb on + strand at 330.311 kb on - strand at 330.320 kb on + strand at 330.320 kb on + strand at 330.320 kb on + strand at 330.321 kb on - strand at 330.338 kb on + strand at 330.338 kb on + strand at 330.338 kb on + strand at 330.338 kb on + strand at 330.339 kb on - strand at 330.339 kb on - strand at 330.339 kb on - strand at 330.339 kb on - strand at 330.352 kb on + strand at 330.352 kb on + strand at 330.352 kb on + strand at 330.353 kb on - strand at 330.353 kb on - strand at 330.461 kb on + strand, within EX31_RS04040 at 330.462 kb on - strand, within EX31_RS04040 at 330.601 kb on + strand, within EX31_RS04040 at 330.616 kb on + strand, within EX31_RS04040 at 330.617 kb on - strand, within EX31_RS04040 at 330.651 kb on - strand, within EX31_RS04040 at 330.740 kb on + strand, within EX31_RS04040 at 330.741 kb on - strand, within EX31_RS04040 at 330.741 kb on - strand, within EX31_RS04040 at 330.741 kb on - strand, within EX31_RS04040 at 330.741 kb on - strand, within EX31_RS04040 at 330.741 kb on - strand, within EX31_RS04040 at 330.775 kb on + strand, within EX31_RS04040 at 330.775 kb on + strand, within EX31_RS04040 at 330.776 kb on - strand, within EX31_RS04040 at 330.805 kb on + strand, within EX31_RS04040 at 330.905 kb on - strand, within EX31_RS04040 at 330.905 kb on - strand, within EX31_RS04040 at 330.905 kb on - strand, within EX31_RS04040 at 330.905 kb on - strand, within EX31_RS04040 at 331.124 kb on + strand at 331.125 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 328,342 - EX31_RS04030 0.42 -1.0 328,342 - EX31_RS04030 0.42 -0.2 328,342 - EX31_RS04030 0.42 +0.7 328,439 + EX31_RS04030 0.49 +0.7 328,439 + EX31_RS04030 0.49 +0.2 328,440 - EX31_RS04030 0.49 +0.6 328,440 - EX31_RS04030 0.49 +0.3 328,481 + EX31_RS04030 0.52 -0.3 328,482 - EX31_RS04030 0.52 -0.4 328,540 + EX31_RS04030 0.56 -0.1 328,541 - EX31_RS04030 0.56 -0.8 328,691 + EX31_RS04030 0.67 -1.2 328,691 + EX31_RS04030 0.67 +0.8 328,691 + EX31_RS04030 0.67 -0.5 328,691 + EX31_RS04030 0.67 -0.4 328,691 + EX31_RS04030 0.67 +1.3 328,691 + EX31_RS04030 0.67 +0.7 328,691 + EX31_RS04030 0.67 +0.9 328,691 + EX31_RS04030 0.67 +1.0 328,691 + EX31_RS04030 0.67 +0.6 328,691 + EX31_RS04030 0.67 +0.6 328,691 + EX31_RS04030 0.67 -0.6 328,692 - EX31_RS04030 0.67 +0.0 328,692 - EX31_RS04030 0.67 +0.0 328,692 - EX31_RS04030 0.67 +0.6 328,692 - EX31_RS04030 0.67 -0.4 328,692 - EX31_RS04030 0.67 +0.6 328,692 - EX31_RS04030 0.67 +4.4 328,692 - EX31_RS04030 0.67 -1.5 328,692 - EX31_RS04030 0.67 +0.1 328,701 - EX31_RS04030 0.68 -1.2 328,701 - EX31_RS04030 0.68 +0.1 328,701 - EX31_RS04030 0.68 -0.5 328,768 + EX31_RS04030 0.73 -0.7 328,768 + EX31_RS04030 0.73 -0.5 328,768 + EX31_RS04030 0.73 -0.1 328,769 - EX31_RS04030 0.73 +0.0 328,769 - EX31_RS04030 0.73 +0.3 328,769 - EX31_RS04030 0.73 -0.5 328,820 + EX31_RS04030 0.76 -0.2 328,821 - EX31_RS04030 0.76 -0.6 328,821 - EX31_RS04030 0.76 -0.7 328,821 - EX31_RS04030 0.76 -0.9 328,872 + EX31_RS04030 0.80 -0.8 328,873 - EX31_RS04030 0.80 +0.4 328,884 + EX31_RS04030 0.81 -1.1 328,885 - EX31_RS04030 0.81 -0.6 328,885 - EX31_RS04030 0.81 -1.5 328,885 - EX31_RS04030 0.81 +1.2 328,887 + EX31_RS04030 0.81 +0.5 328,887 + EX31_RS04030 0.81 +0.6 328,887 + EX31_RS04030 0.81 +0.1 328,887 + EX31_RS04030 0.81 -0.3 328,887 + EX31_RS04030 0.81 +0.3 328,888 - EX31_RS04030 0.81 +0.2 328,888 - EX31_RS04030 0.81 +0.9 328,888 - EX31_RS04030 0.81 +0.5 328,888 - EX31_RS04030 0.81 -1.0 328,888 - EX31_RS04030 0.81 +1.2 328,888 - EX31_RS04030 0.81 -0.6 328,888 - EX31_RS04030 0.81 -0.5 328,905 - EX31_RS04030 0.82 -0.1 328,944 - EX31_RS04030 0.85 +0.2 329,006 + EX31_RS04030 0.90 -0.5 329,073 - +0.3 329,073 - -1.1 329,073 - -0.4 329,073 - -0.3 329,073 - -0.1 329,073 - -0.3 329,073 - +0.7 329,095 + +1.8 329,152 + -1.9 329,152 + +0.2 329,152 + -0.8 329,153 - +0.5 329,153 - -0.4 329,153 - -0.8 329,153 - -1.8 329,153 - +0.1 329,153 - -0.2 329,153 - -0.2 329,153 - +0.8 329,153 - +0.5 329,153 - +1.2 329,153 - -0.3 329,153 - -0.2 329,153 - -0.4 329,153 - +0.3 329,162 + -0.2 329,163 - +0.9 329,163 - +0.5 329,163 - -0.3 329,163 - +0.1 329,163 - +0.3 329,170 - -0.3 329,186 + +1.8 329,187 - -1.0 329,187 - -1.4 329,187 - +0.5 329,228 + -3.4 329,228 + -2.0 329,228 + -2.4 329,245 - -2.5 329,266 - EX31_RS04035 0.10 -3.0 329,266 - EX31_RS04035 0.10 -2.0 329,266 - EX31_RS04035 0.10 -2.8 329,376 + EX31_RS04035 0.22 -2.5 329,376 + EX31_RS04035 0.22 -3.7 329,377 + EX31_RS04035 0.22 -3.1 329,378 - EX31_RS04035 0.22 -3.7 329,378 - EX31_RS04035 0.22 -4.7 329,386 + EX31_RS04035 0.23 -1.8 329,386 + EX31_RS04035 0.23 -2.4 329,386 + EX31_RS04035 0.23 -2.3 329,386 + EX31_RS04035 0.23 -2.7 329,386 + EX31_RS04035 0.23 -3.9 329,386 + EX31_RS04035 0.23 -1.9 329,386 + EX31_RS04035 0.23 -3.4 329,388 + EX31_RS04035 0.23 -1.4 329,388 + EX31_RS04035 0.23 -2.7 329,388 + EX31_RS04035 0.23 -2.4 329,388 + EX31_RS04035 0.23 -1.9 329,389 - EX31_RS04035 0.23 -3.5 329,389 - EX31_RS04035 0.23 -3.0 329,389 - EX31_RS04035 0.23 -3.3 329,389 - EX31_RS04035 0.23 -2.9 329,389 - EX31_RS04035 0.23 -4.3 329,389 - EX31_RS04035 0.23 -3.5 329,431 + EX31_RS04035 0.27 -2.7 329,493 + EX31_RS04035 0.34 -2.4 329,503 - EX31_RS04035 0.35 -2.9 329,503 - EX31_RS04035 0.35 -3.4 329,505 + EX31_RS04035 0.35 -4.4 329,505 + EX31_RS04035 0.35 -3.4 329,505 + EX31_RS04035 0.35 -1.8 329,506 - EX31_RS04035 0.35 -2.0 329,506 - EX31_RS04035 0.35 -1.9 329,506 - EX31_RS04035 0.35 -4.9 329,506 - EX31_RS04035 0.35 -1.9 329,506 - EX31_RS04035 0.35 -1.8 329,506 - EX31_RS04035 0.35 -3.2 329,506 - EX31_RS04035 0.35 -2.9 329,506 - EX31_RS04035 0.35 -1.0 329,506 - EX31_RS04035 0.35 -1.9 329,506 - EX31_RS04035 0.35 -3.7 329,506 - EX31_RS04035 0.35 -2.5 329,506 - EX31_RS04035 0.35 -4.0 329,509 + EX31_RS04035 0.35 -1.8 329,509 + EX31_RS04035 0.35 -4.1 329,509 + EX31_RS04035 0.35 -2.4 329,774 - EX31_RS04035 0.63 -1.8 329,870 + EX31_RS04035 0.73 -1.9 329,870 + EX31_RS04035 0.73 -2.7 329,870 + EX31_RS04035 0.73 -3.9 329,871 - EX31_RS04035 0.73 -3.7 329,871 - EX31_RS04035 0.73 -2.9 329,871 - EX31_RS04035 0.73 -2.7 329,871 - EX31_RS04035 0.73 -2.0 329,871 - EX31_RS04035 0.73 -3.7 329,871 - EX31_RS04035 0.73 -1.5 329,871 - EX31_RS04035 0.73 -2.5 329,881 + EX31_RS04035 0.74 -3.1 329,910 + EX31_RS04035 0.77 -3.5 329,974 + EX31_RS04035 0.83 -2.8 329,974 + EX31_RS04035 0.83 -1.3 329,974 + EX31_RS04035 0.83 -2.6 329,974 + EX31_RS04035 0.83 -3.4 329,974 + EX31_RS04035 0.83 -3.6 329,974 + EX31_RS04035 0.83 -2.4 329,974 + EX31_RS04035 0.83 -3.1 329,974 + EX31_RS04035 0.83 -2.0 329,974 + EX31_RS04035 0.83 -2.0 329,974 + EX31_RS04035 0.83 -2.7 329,974 + EX31_RS04035 0.83 -1.5 329,974 + EX31_RS04035 0.83 -3.0 329,975 - EX31_RS04035 0.84 +0.5 329,975 - EX31_RS04035 0.84 -3.8 329,975 - EX31_RS04035 0.84 -2.4 329,975 - EX31_RS04035 0.84 -3.7 329,977 + EX31_RS04035 0.84 -2.3 329,977 + EX31_RS04035 0.84 -1.0 329,977 + EX31_RS04035 0.84 -3.4 329,978 - EX31_RS04035 0.84 -4.1 329,978 - EX31_RS04035 0.84 -2.6 330,114 + -3.4 330,114 + -4.0 330,114 + -2.0 330,115 - -3.3 330,115 - -1.1 330,115 - -2.0 330,115 - -0.3 330,161 + -2.5 330,174 + -3.6 330,184 + -3.2 330,185 - -0.5 330,185 - +0.7 330,259 - +0.1 330,260 + -3.4 330,260 + -2.3 330,261 - -0.2 330,261 - -2.9 330,310 + -2.2 330,310 + -1.4 330,310 + -3.0 330,310 + -3.0 330,310 + -0.3 330,310 + -3.6 330,311 - -1.3 330,320 + -1.3 330,320 + -1.1 330,320 + -2.2 330,321 - -0.5 330,338 + -0.7 330,338 + +0.3 330,338 + -0.5 330,338 + -0.3 330,339 - +0.1 330,339 - -0.1 330,339 - +0.1 330,339 - -0.5 330,352 + -0.3 330,352 + -0.1 330,352 + +0.4 330,353 - +0.8 330,353 - -1.7 330,461 + EX31_RS04040 0.12 +0.0 330,462 - EX31_RS04040 0.12 -1.0 330,601 + EX31_RS04040 0.30 -0.1 330,616 + EX31_RS04040 0.31 +1.2 330,617 - EX31_RS04040 0.32 +0.2 330,651 - EX31_RS04040 0.36 -0.6 330,740 + EX31_RS04040 0.47 +0.3 330,741 - EX31_RS04040 0.47 +0.7 330,741 - EX31_RS04040 0.47 -0.4 330,741 - EX31_RS04040 0.47 -0.2 330,741 - EX31_RS04040 0.47 +0.7 330,741 - EX31_RS04040 0.47 +0.6 330,775 + EX31_RS04040 0.52 +0.2 330,775 + EX31_RS04040 0.52 -0.5 330,776 - EX31_RS04040 0.52 -0.5 330,805 + EX31_RS04040 0.55 +0.1 330,905 - EX31_RS04040 0.68 +0.5 330,905 - EX31_RS04040 0.68 +1.1 330,905 - EX31_RS04040 0.68 -2.5 330,905 - EX31_RS04040 0.68 +0.3 331,124 + +0.3 331,125 - -2.3
Or see this region's nucleotide sequence