Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03875 and EX31_RS03880 are separated by 439 nucleotides EX31_RS03880 and EX31_RS03885 are separated by 82 nucleotides EX31_RS03885 and EX31_RS03890 are separated by 39 nucleotides
EX31_RS03875: EX31_RS03875 - bifunctional riboflavin kinase/FAD synthetase, at 288,987 to 289,925
_RS03875
EX31_RS03880: EX31_RS03880 - 30S ribosomal protein S20, at 290,365 to 290,628
_RS03880
EX31_RS03885: EX31_RS03885 - transcriptional activator NhaR, at 290,711 to 291,598
_RS03885
EX31_RS03890: EX31_RS03890 - Na+/H+ antiporter NhaA, at 291,638 to 292,813
_RS03890
Position (kb) 290
291
292 Strain fitness (log2 ratio) -2
-1
0
1
2 at 289.926 kb on - strand at 289.926 kb on - strand at 289.975 kb on - strand at 289.977 kb on - strand at 289.977 kb on - strand at 289.977 kb on - strand at 289.983 kb on + strand at 289.983 kb on + strand at 289.984 kb on - strand at 289.984 kb on - strand at 289.984 kb on - strand at 289.984 kb on - strand at 290.009 kb on + strand at 290.038 kb on - strand at 290.046 kb on + strand at 290.047 kb on - strand at 290.093 kb on - strand at 290.093 kb on - strand at 290.093 kb on - strand at 290.093 kb on - strand at 290.139 kb on - strand at 290.152 kb on + strand at 290.152 kb on + strand at 290.152 kb on + strand at 290.272 kb on - strand at 290.682 kb on + strand at 290.786 kb on + strand at 290.787 kb on - strand at 290.793 kb on + strand at 290.793 kb on + strand at 290.794 kb on - strand at 290.794 kb on - strand at 290.794 kb on - strand at 290.796 kb on + strand at 290.796 kb on + strand at 290.797 kb on - strand at 290.797 kb on - strand at 290.797 kb on - strand at 290.797 kb on - strand at 290.797 kb on - strand at 290.806 kb on + strand, within EX31_RS03885 at 290.806 kb on + strand, within EX31_RS03885 at 290.807 kb on - strand, within EX31_RS03885 at 290.866 kb on - strand, within EX31_RS03885 at 290.948 kb on - strand, within EX31_RS03885 at 290.971 kb on + strand, within EX31_RS03885 at 290.971 kb on + strand, within EX31_RS03885 at 291.024 kb on - strand, within EX31_RS03885 at 291.073 kb on + strand, within EX31_RS03885 at 291.267 kb on - strand, within EX31_RS03885 at 291.383 kb on - strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.481 kb on + strand, within EX31_RS03885 at 291.482 kb on - strand, within EX31_RS03885 at 291.509 kb on - strand, within EX31_RS03885 at 291.509 kb on - strand, within EX31_RS03885 at 291.509 kb on - strand, within EX31_RS03885 at 291.551 kb on - strand at 291.570 kb on + strand at 291.571 kb on - strand at 291.571 kb on - strand at 291.571 kb on - strand at 291.582 kb on + strand at 291.582 kb on + strand at 291.583 kb on - strand at 291.583 kb on - strand at 291.583 kb on - strand at 291.589 kb on + strand at 291.589 kb on + strand at 291.590 kb on - strand at 291.619 kb on + strand at 291.620 kb on - strand at 291.637 kb on - strand at 291.637 kb on - strand at 291.639 kb on + strand at 291.640 kb on - strand at 291.640 kb on - strand at 291.656 kb on - strand at 291.733 kb on - strand at 291.863 kb on - strand, within EX31_RS03890 at 291.914 kb on - strand, within EX31_RS03890 at 291.938 kb on - strand, within EX31_RS03890 at 292.024 kb on + strand, within EX31_RS03890 at 292.038 kb on + strand, within EX31_RS03890 at 292.038 kb on + strand, within EX31_RS03890 at 292.082 kb on - strand, within EX31_RS03890 at 292.227 kb on - strand, within EX31_RS03890 at 292.290 kb on + strand, within EX31_RS03890 at 292.290 kb on + strand, within EX31_RS03890 at 292.476 kb on - strand, within EX31_RS03890 at 292.476 kb on - strand, within EX31_RS03890 at 292.480 kb on - strand, within EX31_RS03890 at 292.480 kb on - strand, within EX31_RS03890 at 292.480 kb on - strand, within EX31_RS03890 at 292.484 kb on + strand, within EX31_RS03890 at 292.485 kb on - strand, within EX31_RS03890
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 289,926 - -2.4 289,926 - -1.0 289,975 - +0.6 289,977 - +0.0 289,977 - +0.0 289,977 - -1.0 289,983 + +0.1 289,983 + -1.1 289,984 - -1.9 289,984 - -0.2 289,984 - +0.6 289,984 - +0.2 290,009 + +0.6 290,038 - -0.8 290,046 + -0.3 290,047 - +0.7 290,093 - +0.2 290,093 - +0.7 290,093 - +1.5 290,093 - -0.4 290,139 - -0.9 290,152 + -0.9 290,152 + +0.9 290,152 + +1.0 290,272 - -0.3 290,682 + -0.4 290,786 + -1.0 290,787 - -1.5 290,793 + +0.0 290,793 + -1.4 290,794 - -0.1 290,794 - -0.0 290,794 - -0.8 290,796 + +0.6 290,796 + +2.0 290,797 - +0.5 290,797 - -0.3 290,797 - +0.5 290,797 - -0.6 290,797 - +0.8 290,806 + EX31_RS03885 0.11 +1.0 290,806 + EX31_RS03885 0.11 +0.3 290,807 - EX31_RS03885 0.11 -0.6 290,866 - EX31_RS03885 0.17 -1.4 290,948 - EX31_RS03885 0.27 +0.2 290,971 + EX31_RS03885 0.29 +1.4 290,971 + EX31_RS03885 0.29 +1.1 291,024 - EX31_RS03885 0.35 +0.2 291,073 + EX31_RS03885 0.41 -0.1 291,267 - EX31_RS03885 0.63 -0.5 291,383 - EX31_RS03885 0.76 -0.0 291,481 + EX31_RS03885 0.87 +2.0 291,481 + EX31_RS03885 0.87 +1.0 291,481 + EX31_RS03885 0.87 +1.0 291,481 + EX31_RS03885 0.87 -0.4 291,481 + EX31_RS03885 0.87 -0.2 291,481 + EX31_RS03885 0.87 +1.0 291,482 - EX31_RS03885 0.87 -0.1 291,509 - EX31_RS03885 0.90 +0.5 291,509 - EX31_RS03885 0.90 +0.6 291,509 - EX31_RS03885 0.90 -0.8 291,551 - -0.3 291,570 + -0.6 291,571 - +0.1 291,571 - +0.2 291,571 - +1.1 291,582 + -1.2 291,582 + -0.8 291,583 - -1.0 291,583 - +2.0 291,583 - -1.8 291,589 + -1.9 291,589 + -0.5 291,590 - -0.6 291,619 + +0.1 291,620 - +0.6 291,637 - -0.3 291,637 - +1.0 291,639 + +0.6 291,640 - +0.4 291,640 - -1.7 291,656 - -2.7 291,733 - -1.1 291,863 - EX31_RS03890 0.19 +0.5 291,914 - EX31_RS03890 0.23 +0.6 291,938 - EX31_RS03890 0.26 -1.8 292,024 + EX31_RS03890 0.33 +0.7 292,038 + EX31_RS03890 0.34 -0.0 292,038 + EX31_RS03890 0.34 +0.5 292,082 - EX31_RS03890 0.38 -1.0 292,227 - EX31_RS03890 0.50 -0.5 292,290 + EX31_RS03890 0.55 -0.5 292,290 + EX31_RS03890 0.55 -1.5 292,476 - EX31_RS03890 0.71 +1.3 292,476 - EX31_RS03890 0.71 +0.2 292,480 - EX31_RS03890 0.72 +0.7 292,480 - EX31_RS03890 0.72 -1.1 292,480 - EX31_RS03890 0.72 -1.2 292,484 + EX31_RS03890 0.72 +2.0 292,485 - EX31_RS03890 0.72 -1.4
Or see this region's nucleotide sequence