Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03690 and EX31_RS03695 are separated by 10 nucleotides EX31_RS03695 and EX31_RS03700 are separated by 283 nucleotides EX31_RS03700 and EX31_RS03705 are separated by 227 nucleotides
EX31_RS03690: EX31_RS03690 - 3-isopropylmalate dehydratase large subunit, at 238,075 to 239,475
_RS03690
EX31_RS03695: EX31_RS03695 - 3-isopropylmalate dehydratase small subunit, at 239,486 to 240,088
_RS03695
EX31_RS03700: EX31_RS03700 - hypothetical protein, at 240,372 to 241,064
_RS03700
EX31_RS03705: EX31_RS03705 - MFS transporter, at 241,292 to 242,455
_RS03705
Position (kb) 240
241
242 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 239.492 kb on - strand at 239.536 kb on + strand at 239.536 kb on + strand at 239.536 kb on + strand at 239.536 kb on + strand at 239.537 kb on - strand at 239.563 kb on + strand, within EX31_RS03695 at 239.575 kb on - strand, within EX31_RS03695 at 239.684 kb on + strand, within EX31_RS03695 at 239.685 kb on - strand, within EX31_RS03695 at 239.769 kb on - strand, within EX31_RS03695 at 239.814 kb on - strand, within EX31_RS03695 at 239.897 kb on - strand, within EX31_RS03695 at 239.961 kb on - strand, within EX31_RS03695 at 240.046 kb on + strand at 240.047 kb on - strand at 240.090 kb on + strand at 240.090 kb on + strand at 240.090 kb on + strand at 240.090 kb on + strand at 240.091 kb on - strand at 240.091 kb on - strand at 240.091 kb on - strand at 240.091 kb on - strand at 240.093 kb on + strand at 240.093 kb on + strand at 240.093 kb on + strand at 240.175 kb on - strand at 240.184 kb on + strand at 240.184 kb on + strand at 240.185 kb on - strand at 240.185 kb on - strand at 240.185 kb on - strand at 240.185 kb on - strand at 240.218 kb on - strand at 240.218 kb on - strand at 240.218 kb on - strand at 240.227 kb on - strand at 240.227 kb on - strand at 240.307 kb on - strand at 240.325 kb on - strand at 240.437 kb on - strand at 240.446 kb on + strand, within EX31_RS03700 at 240.456 kb on - strand, within EX31_RS03700 at 240.456 kb on - strand, within EX31_RS03700 at 240.456 kb on - strand, within EX31_RS03700 at 240.524 kb on - strand, within EX31_RS03700 at 240.635 kb on + strand, within EX31_RS03700 at 240.639 kb on + strand, within EX31_RS03700 at 240.639 kb on + strand, within EX31_RS03700 at 240.639 kb on + strand, within EX31_RS03700 at 240.640 kb on - strand, within EX31_RS03700 at 240.640 kb on - strand, within EX31_RS03700 at 240.654 kb on - strand, within EX31_RS03700 at 240.654 kb on - strand, within EX31_RS03700 at 240.654 kb on - strand, within EX31_RS03700 at 240.675 kb on + strand, within EX31_RS03700 at 240.728 kb on - strand, within EX31_RS03700 at 240.799 kb on + strand, within EX31_RS03700 at 240.799 kb on + strand, within EX31_RS03700 at 240.800 kb on - strand, within EX31_RS03700 at 240.800 kb on - strand, within EX31_RS03700 at 240.800 kb on - strand, within EX31_RS03700 at 240.865 kb on - strand, within EX31_RS03700 at 240.943 kb on - strand, within EX31_RS03700 at 240.961 kb on - strand, within EX31_RS03700 at 240.961 kb on - strand, within EX31_RS03700 at 240.961 kb on - strand, within EX31_RS03700 at 240.963 kb on - strand, within EX31_RS03700 at 241.031 kb on + strand at 241.032 kb on - strand at 241.046 kb on + strand at 241.050 kb on + strand at 241.101 kb on + strand at 241.104 kb on + strand at 241.130 kb on - strand at 241.136 kb on - strand at 241.201 kb on + strand at 241.233 kb on + strand at 241.235 kb on + strand at 241.235 kb on + strand at 241.235 kb on + strand at 241.250 kb on + strand at 241.284 kb on + strand at 241.285 kb on - strand at 241.285 kb on - strand at 241.285 kb on - strand at 241.285 kb on - strand at 241.293 kb on + strand at 241.309 kb on + strand at 241.310 kb on - strand at 241.310 kb on - strand at 241.310 kb on - strand at 241.310 kb on - strand at 241.311 kb on + strand at 241.311 kb on + strand at 241.311 kb on + strand at 241.311 kb on + strand at 241.312 kb on - strand at 241.312 kb on - strand at 241.312 kb on - strand at 241.312 kb on - strand at 241.312 kb on - strand at 241.356 kb on + strand at 241.388 kb on - strand at 241.442 kb on + strand, within EX31_RS03705 at 241.448 kb on - strand, within EX31_RS03705 at 241.488 kb on + strand, within EX31_RS03705 at 241.488 kb on + strand, within EX31_RS03705 at 241.488 kb on + strand, within EX31_RS03705 at 241.489 kb on - strand, within EX31_RS03705 at 241.489 kb on - strand, within EX31_RS03705 at 241.489 kb on - strand, within EX31_RS03705 at 241.489 kb on - strand, within EX31_RS03705 at 241.587 kb on + strand, within EX31_RS03705 at 241.614 kb on + strand, within EX31_RS03705 at 241.614 kb on + strand, within EX31_RS03705 at 241.615 kb on - strand, within EX31_RS03705 at 241.653 kb on + strand, within EX31_RS03705 at 241.749 kb on + strand, within EX31_RS03705 at 241.749 kb on + strand, within EX31_RS03705 at 241.750 kb on - strand, within EX31_RS03705 at 241.750 kb on - strand, within EX31_RS03705 at 241.770 kb on + strand, within EX31_RS03705 at 241.770 kb on + strand, within EX31_RS03705 at 241.770 kb on + strand, within EX31_RS03705 at 241.770 kb on + strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.771 kb on - strand, within EX31_RS03705 at 241.887 kb on - strand, within EX31_RS03705 at 241.916 kb on + strand, within EX31_RS03705 at 241.916 kb on + strand, within EX31_RS03705 at 241.916 kb on + strand, within EX31_RS03705 at 241.916 kb on + strand, within EX31_RS03705 at 241.917 kb on - strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.950 kb on + strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.951 kb on - strand, within EX31_RS03705 at 241.968 kb on + strand, within EX31_RS03705 at 241.968 kb on + strand, within EX31_RS03705 at 241.969 kb on - strand, within EX31_RS03705 at 241.969 kb on - strand, within EX31_RS03705 at 241.969 kb on - strand, within EX31_RS03705
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 239,492 - -2.9 239,536 + -2.0 239,536 + -2.8 239,536 + -3.0 239,536 + -1.3 239,537 - -3.2 239,563 + EX31_RS03695 0.13 -3.0 239,575 - EX31_RS03695 0.15 -5.1 239,684 + EX31_RS03695 0.33 -3.0 239,685 - EX31_RS03695 0.33 -2.0 239,769 - EX31_RS03695 0.47 -3.7 239,814 - EX31_RS03695 0.54 -3.0 239,897 - EX31_RS03695 0.68 -3.5 239,961 - EX31_RS03695 0.79 -2.4 240,046 + -3.1 240,047 - -3.4 240,090 + +0.9 240,090 + -2.5 240,090 + -1.0 240,090 + +0.4 240,091 - +0.2 240,091 - -1.7 240,091 - +0.1 240,091 - +0.0 240,093 + -0.4 240,093 + -0.2 240,093 + -0.6 240,175 - -1.2 240,184 + +1.2 240,184 + -0.7 240,185 - +0.2 240,185 - +0.3 240,185 - +0.3 240,185 - +0.1 240,218 - +0.9 240,218 - +0.4 240,218 - -0.3 240,227 - +0.3 240,227 - +1.6 240,307 - -0.0 240,325 - +0.2 240,437 - -0.0 240,446 + EX31_RS03700 0.11 -0.4 240,456 - EX31_RS03700 0.12 -0.0 240,456 - EX31_RS03700 0.12 +0.3 240,456 - EX31_RS03700 0.12 -1.0 240,524 - EX31_RS03700 0.22 +0.8 240,635 + EX31_RS03700 0.38 +0.2 240,639 + EX31_RS03700 0.39 -0.5 240,639 + EX31_RS03700 0.39 +1.4 240,639 + EX31_RS03700 0.39 +0.6 240,640 - EX31_RS03700 0.39 -1.9 240,640 - EX31_RS03700 0.39 -0.8 240,654 - EX31_RS03700 0.41 -3.3 240,654 - EX31_RS03700 0.41 +0.4 240,654 - EX31_RS03700 0.41 -1.3 240,675 + EX31_RS03700 0.44 +0.0 240,728 - EX31_RS03700 0.51 -0.4 240,799 + EX31_RS03700 0.62 -0.2 240,799 + EX31_RS03700 0.62 +0.5 240,800 - EX31_RS03700 0.62 +0.2 240,800 - EX31_RS03700 0.62 +0.0 240,800 - EX31_RS03700 0.62 -0.5 240,865 - EX31_RS03700 0.71 -0.9 240,943 - EX31_RS03700 0.82 +0.5 240,961 - EX31_RS03700 0.85 +0.3 240,961 - EX31_RS03700 0.85 -0.2 240,961 - EX31_RS03700 0.85 +0.4 240,963 - EX31_RS03700 0.85 -0.1 241,031 + +0.8 241,032 - +0.4 241,046 + +0.3 241,050 + -1.0 241,101 + -0.7 241,104 + -0.3 241,130 - +0.1 241,136 - -1.3 241,201 + +0.4 241,233 + +0.4 241,235 + -0.3 241,235 + -0.2 241,235 + +1.0 241,250 + +0.2 241,284 + -0.5 241,285 - +0.5 241,285 - +1.0 241,285 - -0.7 241,285 - +0.6 241,293 + -0.2 241,309 + +0.7 241,310 - +0.7 241,310 - +0.5 241,310 - +1.0 241,310 - +0.4 241,311 + +0.7 241,311 + -0.4 241,311 + -0.5 241,311 + +2.0 241,312 - -0.5 241,312 - -0.2 241,312 - -0.6 241,312 - -0.1 241,312 - +0.3 241,356 + +0.5 241,388 - -0.5 241,442 + EX31_RS03705 0.13 +0.6 241,448 - EX31_RS03705 0.13 +1.6 241,488 + EX31_RS03705 0.17 -0.6 241,488 + EX31_RS03705 0.17 -0.3 241,488 + EX31_RS03705 0.17 -0.9 241,489 - EX31_RS03705 0.17 -0.1 241,489 - EX31_RS03705 0.17 -1.3 241,489 - EX31_RS03705 0.17 -0.2 241,489 - EX31_RS03705 0.17 -1.8 241,587 + EX31_RS03705 0.25 +1.0 241,614 + EX31_RS03705 0.28 -0.5 241,614 + EX31_RS03705 0.28 +1.0 241,615 - EX31_RS03705 0.28 -0.5 241,653 + EX31_RS03705 0.31 -0.1 241,749 + EX31_RS03705 0.39 +0.6 241,749 + EX31_RS03705 0.39 -0.5 241,750 - EX31_RS03705 0.39 -0.6 241,750 - EX31_RS03705 0.39 -2.8 241,770 + EX31_RS03705 0.41 +0.8 241,770 + EX31_RS03705 0.41 +1.2 241,770 + EX31_RS03705 0.41 +0.7 241,770 + EX31_RS03705 0.41 +0.4 241,771 - EX31_RS03705 0.41 -0.1 241,771 - EX31_RS03705 0.41 -0.0 241,771 - EX31_RS03705 0.41 -1.4 241,771 - EX31_RS03705 0.41 -0.2 241,771 - EX31_RS03705 0.41 -0.5 241,771 - EX31_RS03705 0.41 +0.5 241,771 - EX31_RS03705 0.41 -0.3 241,771 - EX31_RS03705 0.41 -2.1 241,771 - EX31_RS03705 0.41 -0.7 241,887 - EX31_RS03705 0.51 +0.8 241,916 + EX31_RS03705 0.54 -0.3 241,916 + EX31_RS03705 0.54 +1.8 241,916 + EX31_RS03705 0.54 +0.3 241,916 + EX31_RS03705 0.54 +0.2 241,917 - EX31_RS03705 0.54 -0.1 241,950 + EX31_RS03705 0.57 -0.1 241,950 + EX31_RS03705 0.57 -0.4 241,950 + EX31_RS03705 0.57 +1.4 241,950 + EX31_RS03705 0.57 -2.5 241,950 + EX31_RS03705 0.57 -0.1 241,950 + EX31_RS03705 0.57 +0.4 241,950 + EX31_RS03705 0.57 +0.1 241,950 + EX31_RS03705 0.57 -1.8 241,951 - EX31_RS03705 0.57 -1.9 241,951 - EX31_RS03705 0.57 -1.0 241,951 - EX31_RS03705 0.57 +0.4 241,951 - EX31_RS03705 0.57 -1.0 241,951 - EX31_RS03705 0.57 -1.1 241,951 - EX31_RS03705 0.57 -0.8 241,951 - EX31_RS03705 0.57 +0.3 241,968 + EX31_RS03705 0.58 +0.7 241,968 + EX31_RS03705 0.58 -0.3 241,969 - EX31_RS03705 0.58 +0.1 241,969 - EX31_RS03705 0.58 +0.2 241,969 - EX31_RS03705 0.58 -0.5
Or see this region's nucleotide sequence