Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03635 and EX31_RS03640 are separated by 27 nucleotides EX31_RS03640 and EX31_RS03645 overlap by 4 nucleotides EX31_RS03645 and EX31_RS03650 are separated by 2 nucleotides
EX31_RS03635: EX31_RS03635 - peptidoglycan glycosyltransferase FtsI, at 223,586 to 225,349
_RS03635
EX31_RS03640: EX31_RS03640 - cell division protein FtsL, at 225,377 to 225,697
_RS03640
EX31_RS03645: EX31_RS03645 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH, at 225,694 to 226,635
_RS03645
EX31_RS03650: EX31_RS03650 - division/cell wall cluster transcriptional repressor MraZ, at 226,638 to 227,096
_RS03650
Position (kb) 225
226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 226.282 kb on - strand, within EX31_RS03645 at 226.331 kb on - strand, within EX31_RS03645 at 226.343 kb on - strand, within EX31_RS03645 at 226.383 kb on - strand, within EX31_RS03645 at 226.557 kb on - strand at 226.640 kb on - strand at 226.640 kb on - strand at 226.640 kb on - strand at 226.640 kb on - strand at 226.640 kb on - strand at 226.640 kb on - strand at 226.642 kb on - strand at 226.721 kb on - strand, within EX31_RS03650 at 226.752 kb on - strand, within EX31_RS03650 at 226.902 kb on - strand, within EX31_RS03650 at 226.902 kb on - strand, within EX31_RS03650 at 226.902 kb on - strand, within EX31_RS03650 at 226.902 kb on - strand, within EX31_RS03650 at 226.902 kb on - strand, within EX31_RS03650 at 226.984 kb on - strand, within EX31_RS03650 at 226.984 kb on - strand, within EX31_RS03650 at 226.984 kb on - strand, within EX31_RS03650 at 227.023 kb on - strand, within EX31_RS03650 at 227.023 kb on - strand, within EX31_RS03650 at 227.212 kb on + strand at 227.213 kb on - strand at 227.216 kb on + strand at 227.277 kb on + strand at 227.278 kb on - strand at 227.316 kb on - strand at 227.349 kb on - strand at 227.352 kb on + strand at 227.352 kb on + strand at 227.353 kb on - strand at 227.353 kb on - strand at 227.356 kb on + strand at 227.356 kb on + strand at 227.356 kb on + strand at 227.356 kb on + strand at 227.356 kb on + strand at 227.357 kb on - strand at 227.357 kb on - strand at 227.357 kb on - strand at 227.357 kb on - strand at 227.357 kb on - strand at 227.357 kb on - strand at 227.360 kb on + strand at 227.361 kb on - strand at 227.361 kb on - strand at 227.361 kb on - strand at 227.363 kb on - strand at 227.445 kb on + strand at 227.482 kb on + strand at 227.483 kb on - strand at 227.500 kb on + strand at 227.543 kb on - strand at 227.556 kb on + strand at 227.556 kb on + strand at 227.556 kb on + strand at 227.557 kb on - strand at 227.557 kb on - strand at 227.557 kb on - strand at 227.579 kb on + strand at 227.584 kb on + strand at 227.585 kb on - strand at 227.585 kb on - strand at 227.585 kb on - strand at 227.585 kb on - strand at 227.609 kb on + strand at 227.609 kb on + strand at 227.609 kb on + strand at 227.609 kb on + strand at 227.612 kb on - strand at 227.612 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 226,282 - EX31_RS03645 0.62 -1.3 226,331 - EX31_RS03645 0.68 -1.4 226,343 - EX31_RS03645 0.69 +1.0 226,383 - EX31_RS03645 0.73 -1.9 226,557 - -0.3 226,640 - +0.5 226,640 - -0.2 226,640 - +1.1 226,640 - +0.4 226,640 - -0.3 226,640 - +1.0 226,642 - -0.5 226,721 - EX31_RS03650 0.18 -0.1 226,752 - EX31_RS03650 0.25 +0.3 226,902 - EX31_RS03650 0.58 +0.5 226,902 - EX31_RS03650 0.58 -0.8 226,902 - EX31_RS03650 0.58 +0.7 226,902 - EX31_RS03650 0.58 -0.4 226,902 - EX31_RS03650 0.58 -1.3 226,984 - EX31_RS03650 0.75 +0.3 226,984 - EX31_RS03650 0.75 -1.1 226,984 - EX31_RS03650 0.75 -3.0 227,023 - EX31_RS03650 0.84 +0.4 227,023 - EX31_RS03650 0.84 -0.4 227,212 + +0.8 227,213 - -0.4 227,216 + +0.9 227,277 + -2.6 227,278 - -0.4 227,316 - -0.1 227,349 - +0.1 227,352 + -1.2 227,352 + +0.9 227,353 - -0.0 227,353 - +0.2 227,356 + -1.3 227,356 + +1.4 227,356 + +1.3 227,356 + +0.2 227,356 + -0.1 227,357 - +1.0 227,357 - -1.6 227,357 - -0.1 227,357 - -0.6 227,357 - +0.2 227,357 - -0.5 227,360 + +2.6 227,361 - -0.8 227,361 - -0.3 227,361 - -0.5 227,363 - +0.5 227,445 + +0.0 227,482 + +0.1 227,483 - -0.3 227,500 + -0.7 227,543 - +0.6 227,556 + +0.8 227,556 + +0.6 227,556 + +0.5 227,557 - +0.5 227,557 - -0.7 227,557 - +1.6 227,579 + -0.3 227,584 + +0.6 227,585 - -0.3 227,585 - +0.5 227,585 - +0.1 227,585 - +0.9 227,609 + -0.0 227,609 + +0.3 227,609 + -0.7 227,609 + -0.3 227,612 - +1.6 227,612 - +2.0
Or see this region's nucleotide sequence