Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02520 and EX31_RS02525 are separated by 47 nucleotides EX31_RS02525 and EX31_RS02530 are separated by 1 nucleotides EX31_RS02530 and EX31_RS02535 are separated by 58 nucleotides
EX31_RS02520: EX31_RS02520 - sigma-54-dependent Fis family transcriptional regulator, at 533,730 to 535,142
_RS02520
EX31_RS02525: EX31_RS02525 - ABC transporter permease, at 535,190 to 537,619
_RS02525
EX31_RS02530: EX31_RS02530 - ABC transporter ATP-binding protein, at 537,621 to 538,337
_RS02530
EX31_RS02535: EX31_RS02535 - HlyD family efflux transporter periplasmic adaptor subunit, at 538,396 to 539,646
_RS02535
Position (kb) 535
536
537
538 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 534.388 kb on - strand, within EX31_RS02520 at 534.388 kb on - strand, within EX31_RS02520 at 534.388 kb on - strand, within EX31_RS02520 at 534.555 kb on - strand, within EX31_RS02520 at 534.557 kb on - strand, within EX31_RS02520 at 534.645 kb on + strand, within EX31_RS02520 at 534.790 kb on - strand, within EX31_RS02520 at 535.058 kb on - strand at 535.316 kb on - strand at 535.316 kb on - strand at 535.316 kb on - strand at 535.333 kb on + strand at 535.334 kb on - strand at 535.334 kb on - strand at 535.334 kb on - strand at 535.334 kb on - strand at 535.334 kb on - strand at 535.395 kb on - strand at 535.403 kb on - strand at 535.460 kb on - strand, within EX31_RS02525 at 535.480 kb on + strand, within EX31_RS02525 at 535.481 kb on - strand, within EX31_RS02525 at 535.481 kb on - strand, within EX31_RS02525 at 535.512 kb on + strand, within EX31_RS02525 at 535.513 kb on - strand, within EX31_RS02525 at 535.513 kb on - strand, within EX31_RS02525 at 535.513 kb on - strand, within EX31_RS02525 at 535.559 kb on - strand, within EX31_RS02525 at 535.559 kb on - strand, within EX31_RS02525 at 535.559 kb on - strand, within EX31_RS02525 at 535.559 kb on - strand, within EX31_RS02525 at 535.559 kb on - strand, within EX31_RS02525 at 535.687 kb on + strand, within EX31_RS02525 at 535.753 kb on - strand, within EX31_RS02525 at 535.804 kb on + strand, within EX31_RS02525 at 536.041 kb on - strand, within EX31_RS02525 at 536.057 kb on - strand, within EX31_RS02525 at 536.057 kb on - strand, within EX31_RS02525 at 536.181 kb on + strand, within EX31_RS02525 at 536.182 kb on - strand, within EX31_RS02525 at 536.182 kb on - strand, within EX31_RS02525 at 536.182 kb on - strand, within EX31_RS02525 at 536.182 kb on - strand, within EX31_RS02525 at 536.315 kb on + strand, within EX31_RS02525 at 536.316 kb on - strand, within EX31_RS02525 at 536.316 kb on - strand, within EX31_RS02525 at 536.316 kb on - strand, within EX31_RS02525 at 536.496 kb on - strand, within EX31_RS02525 at 536.578 kb on + strand, within EX31_RS02525 at 536.578 kb on + strand, within EX31_RS02525 at 536.579 kb on - strand, within EX31_RS02525 at 536.646 kb on - strand, within EX31_RS02525 at 536.747 kb on + strand, within EX31_RS02525 at 536.748 kb on - strand, within EX31_RS02525 at 536.748 kb on - strand, within EX31_RS02525 at 536.858 kb on + strand, within EX31_RS02525 at 537.009 kb on + strand, within EX31_RS02525 at 537.028 kb on + strand, within EX31_RS02525 at 537.172 kb on - strand, within EX31_RS02525 at 537.289 kb on + strand, within EX31_RS02525 at 537.291 kb on + strand, within EX31_RS02525 at 537.291 kb on + strand, within EX31_RS02525 at 537.292 kb on - strand, within EX31_RS02525 at 537.292 kb on - strand, within EX31_RS02525 at 537.336 kb on + strand, within EX31_RS02525 at 537.364 kb on + strand, within EX31_RS02525 at 537.365 kb on - strand, within EX31_RS02525 at 537.444 kb on - strand at 537.514 kb on + strand at 537.515 kb on - strand at 537.516 kb on + strand at 537.517 kb on - strand at 537.517 kb on - strand at 537.547 kb on + strand at 537.560 kb on - strand at 537.560 kb on - strand at 537.623 kb on - strand at 537.623 kb on - strand at 537.633 kb on + strand at 537.770 kb on + strand, within EX31_RS02530 at 537.935 kb on + strand, within EX31_RS02530 at 537.935 kb on + strand, within EX31_RS02530 at 537.936 kb on - strand, within EX31_RS02530 at 537.936 kb on - strand, within EX31_RS02530 at 537.990 kb on - strand, within EX31_RS02530 at 537.990 kb on - strand, within EX31_RS02530 at 538.144 kb on + strand, within EX31_RS02530 at 538.145 kb on - strand, within EX31_RS02530 at 538.145 kb on - strand, within EX31_RS02530 at 538.200 kb on - strand, within EX31_RS02530 at 538.324 kb on - strand at 538.365 kb on + strand at 538.398 kb on - strand at 538.398 kb on - strand at 538.398 kb on - strand at 538.402 kb on - strand at 538.492 kb on - strand at 538.492 kb on - strand at 538.492 kb on - strand at 538.494 kb on - strand at 538.494 kb on - strand at 538.494 kb on - strand at 538.494 kb on - strand at 538.611 kb on - strand, within EX31_RS02535 at 538.611 kb on - strand, within EX31_RS02535
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 534,388 - EX31_RS02520 0.47 -1.9 534,388 - EX31_RS02520 0.47 -1.7 534,388 - EX31_RS02520 0.47 -1.2 534,555 - EX31_RS02520 0.58 -2.5 534,557 - EX31_RS02520 0.59 -2.1 534,645 + EX31_RS02520 0.65 -1.6 534,790 - EX31_RS02520 0.75 -2.6 535,058 - -0.5 535,316 - -1.1 535,316 - +0.4 535,316 - -2.3 535,333 + -0.9 535,334 - -0.3 535,334 - -1.6 535,334 - -1.2 535,334 - +1.1 535,334 - -2.0 535,395 - -0.8 535,403 - -1.1 535,460 - EX31_RS02525 0.11 -2.1 535,480 + EX31_RS02525 0.12 -1.2 535,481 - EX31_RS02525 0.12 -1.9 535,481 - EX31_RS02525 0.12 +0.3 535,512 + EX31_RS02525 0.13 -1.3 535,513 - EX31_RS02525 0.13 -1.1 535,513 - EX31_RS02525 0.13 -1.3 535,513 - EX31_RS02525 0.13 -2.3 535,559 - EX31_RS02525 0.15 +0.1 535,559 - EX31_RS02525 0.15 -1.3 535,559 - EX31_RS02525 0.15 +0.2 535,559 - EX31_RS02525 0.15 +1.0 535,559 - EX31_RS02525 0.15 -1.3 535,687 + EX31_RS02525 0.20 -0.5 535,753 - EX31_RS02525 0.23 -0.6 535,804 + EX31_RS02525 0.25 -0.9 536,041 - EX31_RS02525 0.35 +0.7 536,057 - EX31_RS02525 0.36 -1.6 536,057 - EX31_RS02525 0.36 -1.1 536,181 + EX31_RS02525 0.41 -0.9 536,182 - EX31_RS02525 0.41 -0.5 536,182 - EX31_RS02525 0.41 +0.6 536,182 - EX31_RS02525 0.41 -1.3 536,182 - EX31_RS02525 0.41 +1.8 536,315 + EX31_RS02525 0.46 -1.8 536,316 - EX31_RS02525 0.46 -1.1 536,316 - EX31_RS02525 0.46 +0.8 536,316 - EX31_RS02525 0.46 +0.1 536,496 - EX31_RS02525 0.54 +0.9 536,578 + EX31_RS02525 0.57 -1.0 536,578 + EX31_RS02525 0.57 -1.3 536,579 - EX31_RS02525 0.57 +0.5 536,646 - EX31_RS02525 0.60 -1.5 536,747 + EX31_RS02525 0.64 -0.7 536,748 - EX31_RS02525 0.64 -0.9 536,748 - EX31_RS02525 0.64 -0.7 536,858 + EX31_RS02525 0.69 -2.4 537,009 + EX31_RS02525 0.75 -2.0 537,028 + EX31_RS02525 0.76 -2.2 537,172 - EX31_RS02525 0.82 -1.1 537,289 + EX31_RS02525 0.86 -1.1 537,291 + EX31_RS02525 0.86 -2.2 537,291 + EX31_RS02525 0.86 -2.1 537,292 - EX31_RS02525 0.87 -1.5 537,292 - EX31_RS02525 0.87 -1.7 537,336 + EX31_RS02525 0.88 -1.2 537,364 + EX31_RS02525 0.89 -2.3 537,365 - EX31_RS02525 0.90 -0.9 537,444 - -1.2 537,514 + -0.5 537,515 - -0.7 537,516 + -2.8 537,517 - -2.6 537,517 - +0.3 537,547 + -1.7 537,560 - -2.5 537,560 - +0.1 537,623 - -0.7 537,623 - -0.3 537,633 + +0.1 537,770 + EX31_RS02530 0.21 -2.8 537,935 + EX31_RS02530 0.44 -1.1 537,935 + EX31_RS02530 0.44 -2.7 537,936 - EX31_RS02530 0.44 -1.2 537,936 - EX31_RS02530 0.44 -3.2 537,990 - EX31_RS02530 0.51 -2.6 537,990 - EX31_RS02530 0.51 -3.5 538,144 + EX31_RS02530 0.73 -2.4 538,145 - EX31_RS02530 0.73 -2.0 538,145 - EX31_RS02530 0.73 -1.9 538,200 - EX31_RS02530 0.81 -2.6 538,324 - +0.7 538,365 + -1.3 538,398 - +0.3 538,398 - +0.4 538,398 - -0.1 538,402 - -0.5 538,492 - +0.2 538,492 - -0.3 538,492 - -1.0 538,494 - -0.5 538,494 - -0.6 538,494 - +1.4 538,494 - -0.1 538,611 - EX31_RS02535 0.17 -1.1 538,611 - EX31_RS02535 0.17 -0.3
Or see this region's nucleotide sequence