Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02375 and EX31_RS02380 are separated by 62 nucleotides EX31_RS02380 and EX31_RS02385 are separated by 134 nucleotides EX31_RS02385 and EX31_RS02390 are separated by 125 nucleotides EX31_RS02390 and EX31_RS02395 are separated by 134 nucleotides
EX31_RS02375: EX31_RS02375 - type II toxin-antitoxin system RelE/ParE family toxin, at 508,083 to 508,430
_RS02375
EX31_RS02380: EX31_RS02380 - OsmC family protein, at 508,493 to 508,924
_RS02380
EX31_RS02385: EX31_RS02385 - hypothetical protein, at 509,059 to 509,238
_RS02385
EX31_RS02390: EX31_RS02390 - steroid Delta-isomerase, at 509,364 to 509,699
_RS02390
EX31_RS02395: EX31_RS02395 - hypothetical protein, at 509,834 to 510,193
_RS02395
Position (kb) 509
510 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 508.082 kb on + strand at 508.100 kb on + strand at 508.116 kb on + strand at 508.116 kb on + strand at 508.129 kb on + strand, within EX31_RS02375 at 508.135 kb on + strand, within EX31_RS02375 at 508.186 kb on + strand, within EX31_RS02375 at 508.204 kb on + strand, within EX31_RS02375 at 508.204 kb on + strand, within EX31_RS02375 at 508.204 kb on + strand, within EX31_RS02375 at 508.207 kb on + strand, within EX31_RS02375 at 508.207 kb on + strand, within EX31_RS02375 at 508.207 kb on + strand, within EX31_RS02375 at 508.207 kb on + strand, within EX31_RS02375 at 508.208 kb on - strand, within EX31_RS02375 at 508.234 kb on + strand, within EX31_RS02375 at 508.304 kb on + strand, within EX31_RS02375 at 508.327 kb on + strand, within EX31_RS02375 at 508.327 kb on + strand, within EX31_RS02375 at 508.327 kb on + strand, within EX31_RS02375 at 508.327 kb on + strand, within EX31_RS02375 at 508.327 kb on + strand, within EX31_RS02375 at 508.327 kb on + strand, within EX31_RS02375 at 508.327 kb on + strand, within EX31_RS02375 at 508.327 kb on + strand, within EX31_RS02375 at 508.327 kb on + strand, within EX31_RS02375 at 508.327 kb on + strand, within EX31_RS02375 at 508.327 kb on + strand, within EX31_RS02375 at 508.328 kb on - strand, within EX31_RS02375 at 508.328 kb on - strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.329 kb on + strand, within EX31_RS02375 at 508.330 kb on - strand, within EX31_RS02375 at 508.330 kb on - strand, within EX31_RS02375 at 508.330 kb on - strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.331 kb on + strand, within EX31_RS02375 at 508.332 kb on - strand, within EX31_RS02375 at 508.332 kb on - strand, within EX31_RS02375 at 508.332 kb on - strand, within EX31_RS02375 at 508.332 kb on - strand, within EX31_RS02375 at 508.335 kb on + strand, within EX31_RS02375 at 508.335 kb on + strand, within EX31_RS02375 at 508.335 kb on + strand, within EX31_RS02375 at 508.335 kb on + strand, within EX31_RS02375 at 508.335 kb on + strand, within EX31_RS02375 at 508.365 kb on + strand, within EX31_RS02375 at 508.365 kb on + strand, within EX31_RS02375 at 508.365 kb on + strand, within EX31_RS02375 at 508.366 kb on - strand, within EX31_RS02375 at 508.509 kb on + strand at 508.510 kb on - strand at 508.510 kb on - strand at 508.798 kb on + strand, within EX31_RS02380 at 508.799 kb on - strand, within EX31_RS02380 at 508.799 kb on - strand, within EX31_RS02380 at 508.863 kb on + strand, within EX31_RS02380 at 508.864 kb on - strand, within EX31_RS02380 at 508.864 kb on - strand, within EX31_RS02380 at 508.864 kb on - strand, within EX31_RS02380 at 508.879 kb on + strand, within EX31_RS02380 at 508.924 kb on + strand at 508.945 kb on - strand at 508.955 kb on + strand at 508.955 kb on + strand at 508.956 kb on - strand at 508.959 kb on + strand at 508.959 kb on + strand at 508.959 kb on + strand at 508.959 kb on + strand at 508.959 kb on + strand at 508.959 kb on + strand at 508.960 kb on - strand at 508.960 kb on - strand at 508.960 kb on - strand at 508.960 kb on - strand at 508.960 kb on - strand at 508.960 kb on - strand at 508.961 kb on + strand at 508.961 kb on + strand at 508.961 kb on + strand at 508.961 kb on + strand at 508.961 kb on + strand at 508.962 kb on - strand at 508.962 kb on - strand at 508.962 kb on - strand at 508.962 kb on - strand at 508.962 kb on - strand at 508.962 kb on - strand at 508.962 kb on - strand at 508.962 kb on - strand at 509.038 kb on + strand at 509.043 kb on - strand at 509.053 kb on + strand at 509.064 kb on + strand at 509.183 kb on + strand, within EX31_RS02385 at 509.207 kb on - strand, within EX31_RS02385 at 509.361 kb on + strand at 509.361 kb on + strand at 509.361 kb on + strand at 509.474 kb on + strand, within EX31_RS02390 at 509.488 kb on + strand, within EX31_RS02390 at 509.517 kb on + strand, within EX31_RS02390 at 509.519 kb on + strand, within EX31_RS02390 at 509.549 kb on - strand, within EX31_RS02390 at 509.757 kb on + strand at 509.757 kb on + strand at 509.758 kb on - strand at 509.786 kb on + strand at 509.786 kb on + strand at 509.787 kb on - strand at 509.787 kb on - strand at 509.787 kb on - strand at 509.801 kb on - strand at 509.809 kb on + strand at 509.810 kb on - strand at 509.839 kb on + strand at 509.908 kb on + strand, within EX31_RS02395 at 509.951 kb on + strand, within EX31_RS02395 at 509.951 kb on + strand, within EX31_RS02395 at 509.951 kb on + strand, within EX31_RS02395 at 509.952 kb on - strand, within EX31_RS02395 at 509.983 kb on + strand, within EX31_RS02395 at 510.002 kb on + strand, within EX31_RS02395 at 510.002 kb on + strand, within EX31_RS02395 at 510.002 kb on + strand, within EX31_RS02395 at 510.002 kb on + strand, within EX31_RS02395 at 510.003 kb on - strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.005 kb on - strand, within EX31_RS02395 at 510.005 kb on - strand, within EX31_RS02395 at 510.005 kb on - strand, within EX31_RS02395 at 510.016 kb on + strand, within EX31_RS02395 at 510.035 kb on + strand, within EX31_RS02395 at 510.035 kb on + strand, within EX31_RS02395 at 510.035 kb on + strand, within EX31_RS02395 at 510.035 kb on + strand, within EX31_RS02395 at 510.045 kb on - strand, within EX31_RS02395 at 510.067 kb on + strand, within EX31_RS02395 at 510.102 kb on + strand, within EX31_RS02395 at 510.121 kb on + strand, within EX31_RS02395 at 510.121 kb on + strand, within EX31_RS02395 at 510.121 kb on + strand, within EX31_RS02395 at 510.121 kb on + strand, within EX31_RS02395 at 510.121 kb on + strand, within EX31_RS02395 at 510.122 kb on - strand, within EX31_RS02395 at 510.136 kb on + strand, within EX31_RS02395 at 510.154 kb on + strand, within EX31_RS02395 at 510.188 kb on - strand at 510.191 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 508,082 + +0.2 508,100 + +1.0 508,116 + -0.4 508,116 + -0.3 508,129 + EX31_RS02375 0.13 +0.5 508,135 + EX31_RS02375 0.15 +0.3 508,186 + EX31_RS02375 0.30 -1.3 508,204 + EX31_RS02375 0.35 +0.2 508,204 + EX31_RS02375 0.35 +0.5 508,204 + EX31_RS02375 0.35 -0.9 508,207 + EX31_RS02375 0.36 -0.6 508,207 + EX31_RS02375 0.36 +0.3 508,207 + EX31_RS02375 0.36 +0.5 508,207 + EX31_RS02375 0.36 -0.1 508,208 - EX31_RS02375 0.36 -0.9 508,234 + EX31_RS02375 0.43 -0.2 508,304 + EX31_RS02375 0.64 +0.3 508,327 + EX31_RS02375 0.70 +1.3 508,327 + EX31_RS02375 0.70 -0.5 508,327 + EX31_RS02375 0.70 +0.5 508,327 + EX31_RS02375 0.70 +0.2 508,327 + EX31_RS02375 0.70 +0.4 508,327 + EX31_RS02375 0.70 +0.0 508,327 + EX31_RS02375 0.70 +1.1 508,327 + EX31_RS02375 0.70 +0.4 508,327 + EX31_RS02375 0.70 -0.4 508,327 + EX31_RS02375 0.70 -0.9 508,327 + EX31_RS02375 0.70 +0.2 508,328 - EX31_RS02375 0.70 +0.8 508,328 - EX31_RS02375 0.70 +0.1 508,329 + EX31_RS02375 0.71 +0.3 508,329 + EX31_RS02375 0.71 -0.2 508,329 + EX31_RS02375 0.71 +0.4 508,329 + EX31_RS02375 0.71 -1.2 508,329 + EX31_RS02375 0.71 -0.2 508,329 + EX31_RS02375 0.71 -0.3 508,329 + EX31_RS02375 0.71 -0.6 508,329 + EX31_RS02375 0.71 -0.2 508,329 + EX31_RS02375 0.71 +0.6 508,329 + EX31_RS02375 0.71 +1.0 508,329 + EX31_RS02375 0.71 -0.1 508,329 + EX31_RS02375 0.71 -0.2 508,329 + EX31_RS02375 0.71 -0.0 508,329 + EX31_RS02375 0.71 -1.0 508,329 + EX31_RS02375 0.71 -0.9 508,329 + EX31_RS02375 0.71 +0.3 508,329 + EX31_RS02375 0.71 +2.1 508,329 + EX31_RS02375 0.71 -0.2 508,329 + EX31_RS02375 0.71 -1.3 508,329 + EX31_RS02375 0.71 +1.1 508,329 + EX31_RS02375 0.71 +0.7 508,329 + EX31_RS02375 0.71 +0.2 508,329 + EX31_RS02375 0.71 -1.1 508,329 + EX31_RS02375 0.71 +0.3 508,329 + EX31_RS02375 0.71 +0.3 508,329 + EX31_RS02375 0.71 +0.7 508,329 + EX31_RS02375 0.71 -0.2 508,329 + EX31_RS02375 0.71 -0.6 508,329 + EX31_RS02375 0.71 -0.0 508,329 + EX31_RS02375 0.71 -0.4 508,329 + EX31_RS02375 0.71 +1.5 508,329 + EX31_RS02375 0.71 +1.5 508,330 - EX31_RS02375 0.71 +1.7 508,330 - EX31_RS02375 0.71 -2.1 508,330 - EX31_RS02375 0.71 -0.1 508,331 + EX31_RS02375 0.71 +0.3 508,331 + EX31_RS02375 0.71 +0.2 508,331 + EX31_RS02375 0.71 -0.4 508,331 + EX31_RS02375 0.71 -0.2 508,331 + EX31_RS02375 0.71 +0.4 508,331 + EX31_RS02375 0.71 -0.6 508,331 + EX31_RS02375 0.71 +0.2 508,331 + EX31_RS02375 0.71 +0.0 508,331 + EX31_RS02375 0.71 -0.1 508,331 + EX31_RS02375 0.71 +0.6 508,331 + EX31_RS02375 0.71 -1.1 508,331 + EX31_RS02375 0.71 +0.9 508,331 + EX31_RS02375 0.71 +0.0 508,331 + EX31_RS02375 0.71 +2.1 508,331 + EX31_RS02375 0.71 +0.2 508,331 + EX31_RS02375 0.71 +0.3 508,331 + EX31_RS02375 0.71 -0.7 508,331 + EX31_RS02375 0.71 -0.3 508,332 - EX31_RS02375 0.72 +0.4 508,332 - EX31_RS02375 0.72 -1.5 508,332 - EX31_RS02375 0.72 +2.1 508,332 - EX31_RS02375 0.72 -1.1 508,335 + EX31_RS02375 0.72 +0.6 508,335 + EX31_RS02375 0.72 -1.4 508,335 + EX31_RS02375 0.72 +0.2 508,335 + EX31_RS02375 0.72 +0.3 508,335 + EX31_RS02375 0.72 +0.5 508,365 + EX31_RS02375 0.81 +0.2 508,365 + EX31_RS02375 0.81 -0.2 508,365 + EX31_RS02375 0.81 +0.5 508,366 - EX31_RS02375 0.81 +3.3 508,509 + +1.1 508,510 - +0.7 508,510 - -0.3 508,798 + EX31_RS02380 0.71 -1.3 508,799 - EX31_RS02380 0.71 +0.3 508,799 - EX31_RS02380 0.71 -0.7 508,863 + EX31_RS02380 0.86 -2.6 508,864 - EX31_RS02380 0.86 +0.1 508,864 - EX31_RS02380 0.86 -0.3 508,864 - EX31_RS02380 0.86 -0.7 508,879 + EX31_RS02380 0.89 +0.5 508,924 + +0.3 508,945 - +1.5 508,955 + +1.2 508,955 + +0.1 508,956 - -0.1 508,959 + +1.7 508,959 + +0.3 508,959 + -0.8 508,959 + +0.1 508,959 + +0.9 508,959 + +1.3 508,960 - -0.1 508,960 - +1.0 508,960 - +0.1 508,960 - -0.4 508,960 - +0.5 508,960 - -1.1 508,961 + +1.1 508,961 + -0.3 508,961 + +2.1 508,961 + +0.8 508,961 + +0.4 508,962 - +0.0 508,962 - +0.0 508,962 - -1.4 508,962 - -0.8 508,962 - -0.3 508,962 - +0.3 508,962 - -0.3 508,962 - -0.3 509,038 + +0.1 509,043 - -0.6 509,053 + +0.5 509,064 + +0.4 509,183 + EX31_RS02385 0.69 -0.6 509,207 - EX31_RS02385 0.82 -1.6 509,361 + +0.5 509,361 + +0.4 509,361 + -1.9 509,474 + EX31_RS02390 0.33 -1.2 509,488 + EX31_RS02390 0.37 +1.4 509,517 + EX31_RS02390 0.46 +0.7 509,519 + EX31_RS02390 0.46 +0.1 509,549 - EX31_RS02390 0.55 -1.6 509,757 + +1.5 509,757 + -0.9 509,758 - +0.5 509,786 + -0.1 509,786 + +0.6 509,787 - +1.8 509,787 - -0.1 509,787 - +1.1 509,801 - -0.9 509,809 + +0.9 509,810 - -0.3 509,839 + -0.3 509,908 + EX31_RS02395 0.21 -1.4 509,951 + EX31_RS02395 0.33 +0.7 509,951 + EX31_RS02395 0.33 +0.9 509,951 + EX31_RS02395 0.33 +0.7 509,952 - EX31_RS02395 0.33 +0.4 509,983 + EX31_RS02395 0.41 +1.2 510,002 + EX31_RS02395 0.47 +0.5 510,002 + EX31_RS02395 0.47 -0.3 510,002 + EX31_RS02395 0.47 +0.8 510,002 + EX31_RS02395 0.47 -1.8 510,003 - EX31_RS02395 0.47 +1.1 510,004 + EX31_RS02395 0.47 +1.4 510,004 + EX31_RS02395 0.47 -0.1 510,004 + EX31_RS02395 0.47 -0.1 510,004 + EX31_RS02395 0.47 -1.1 510,004 + EX31_RS02395 0.47 +0.5 510,004 + EX31_RS02395 0.47 +0.0 510,004 + EX31_RS02395 0.47 +0.5 510,005 - EX31_RS02395 0.47 +0.5 510,005 - EX31_RS02395 0.47 -0.9 510,005 - EX31_RS02395 0.47 +0.1 510,016 + EX31_RS02395 0.51 -0.5 510,035 + EX31_RS02395 0.56 +0.4 510,035 + EX31_RS02395 0.56 -2.6 510,035 + EX31_RS02395 0.56 +0.1 510,035 + EX31_RS02395 0.56 +0.1 510,045 - EX31_RS02395 0.59 -1.2 510,067 + EX31_RS02395 0.65 +0.2 510,102 + EX31_RS02395 0.74 -0.4 510,121 + EX31_RS02395 0.80 +0.0 510,121 + EX31_RS02395 0.80 -0.8 510,121 + EX31_RS02395 0.80 +0.6 510,121 + EX31_RS02395 0.80 -0.4 510,121 + EX31_RS02395 0.80 -0.8 510,122 - EX31_RS02395 0.80 +0.1 510,136 + EX31_RS02395 0.84 +0.7 510,154 + EX31_RS02395 0.89 +0.0 510,188 - -1.5 510,191 + +1.5
Or see this region's nucleotide sequence