Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01245 and EX31_RS01250 are separated by 220 nucleotides EX31_RS01250 and EX31_RS01255 are separated by 134 nucleotides EX31_RS01255 and EX31_RS01260 are separated by 52 nucleotides
EX31_RS01245: EX31_RS01245 - ferric-rhodotorulic acid/ferric-coprogen receptor FhuE, at 276,659 to 278,869
_RS01245
EX31_RS01250: EX31_RS01250 - M24 family metallopeptidase, at 279,090 to 280,484
_RS01250
EX31_RS01255: EX31_RS01255 - Crp/Fnr family transcriptional regulator, at 280,619 to 281,251
_RS01255
EX31_RS01260: EX31_RS01260 - YebG family protein, at 281,304 to 281,699
_RS01260
Position (kb) 279
280
281 Strain fitness (log2 ratio) -2
-1
0
1
2 at 278.272 kb on + strand, within EX31_RS01245 at 278.424 kb on + strand, within EX31_RS01245 at 278.425 kb on - strand, within EX31_RS01245 at 278.425 kb on - strand, within EX31_RS01245 at 278.434 kb on + strand, within EX31_RS01245 at 278.434 kb on + strand, within EX31_RS01245 at 278.435 kb on - strand, within EX31_RS01245 at 278.442 kb on + strand, within EX31_RS01245 at 278.443 kb on - strand, within EX31_RS01245 at 278.443 kb on - strand, within EX31_RS01245 at 278.460 kb on + strand, within EX31_RS01245 at 278.460 kb on + strand, within EX31_RS01245 at 278.461 kb on - strand, within EX31_RS01245 at 278.509 kb on + strand, within EX31_RS01245 at 278.509 kb on + strand, within EX31_RS01245 at 278.807 kb on - strand at 278.847 kb on + strand at 278.848 kb on - strand at 278.848 kb on - strand at 278.848 kb on - strand at 278.848 kb on - strand at 278.869 kb on + strand at 278.870 kb on - strand at 278.870 kb on - strand at 278.912 kb on + strand at 278.917 kb on - strand at 278.917 kb on - strand at 278.917 kb on - strand at 279.005 kb on + strand at 279.013 kb on + strand at 279.040 kb on + strand at 279.116 kb on + strand at 279.213 kb on - strand at 279.293 kb on + strand, within EX31_RS01250 at 279.294 kb on - strand, within EX31_RS01250 at 279.336 kb on - strand, within EX31_RS01250 at 279.336 kb on - strand, within EX31_RS01250 at 279.339 kb on - strand, within EX31_RS01250 at 279.388 kb on + strand, within EX31_RS01250 at 279.388 kb on + strand, within EX31_RS01250 at 279.452 kb on - strand, within EX31_RS01250 at 279.452 kb on - strand, within EX31_RS01250 at 279.452 kb on - strand, within EX31_RS01250 at 279.534 kb on + strand, within EX31_RS01250 at 279.547 kb on + strand, within EX31_RS01250 at 279.548 kb on - strand, within EX31_RS01250 at 279.548 kb on - strand, within EX31_RS01250 at 279.595 kb on - strand, within EX31_RS01250 at 279.619 kb on + strand, within EX31_RS01250 at 279.620 kb on - strand, within EX31_RS01250 at 279.671 kb on + strand, within EX31_RS01250 at 279.780 kb on + strand, within EX31_RS01250 at 279.893 kb on + strand, within EX31_RS01250 at 279.893 kb on + strand, within EX31_RS01250 at 279.894 kb on - strand, within EX31_RS01250 at 280.225 kb on + strand, within EX31_RS01250 at 280.225 kb on + strand, within EX31_RS01250 at 280.225 kb on + strand, within EX31_RS01250 at 280.225 kb on + strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.226 kb on - strand, within EX31_RS01250 at 280.294 kb on + strand, within EX31_RS01250 at 280.327 kb on + strand, within EX31_RS01250 at 280.327 kb on + strand, within EX31_RS01250 at 280.328 kb on - strand, within EX31_RS01250 at 280.433 kb on - strand at 280.433 kb on - strand at 280.466 kb on - strand at 280.520 kb on - strand at 280.582 kb on - strand at 280.582 kb on - strand at 280.614 kb on + strand at 280.737 kb on - strand, within EX31_RS01255 at 280.784 kb on - strand, within EX31_RS01255 at 280.960 kb on - strand, within EX31_RS01255 at 281.007 kb on - strand, within EX31_RS01255 at 281.007 kb on - strand, within EX31_RS01255 at 281.120 kb on + strand, within EX31_RS01255 at 281.192 kb on + strand at 281.192 kb on + strand at 281.212 kb on + strand at 281.212 kb on + strand at 281.317 kb on - strand at 281.317 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 278,272 + EX31_RS01245 0.73 -1.8 278,424 + EX31_RS01245 0.80 -0.0 278,425 - EX31_RS01245 0.80 +0.5 278,425 - EX31_RS01245 0.80 +2.1 278,434 + EX31_RS01245 0.80 +0.7 278,434 + EX31_RS01245 0.80 -0.2 278,435 - EX31_RS01245 0.80 +2.1 278,442 + EX31_RS01245 0.81 -0.1 278,443 - EX31_RS01245 0.81 -0.4 278,443 - EX31_RS01245 0.81 +0.5 278,460 + EX31_RS01245 0.81 +0.8 278,460 + EX31_RS01245 0.81 +0.7 278,461 - EX31_RS01245 0.82 +0.7 278,509 + EX31_RS01245 0.84 +2.1 278,509 + EX31_RS01245 0.84 +0.0 278,807 - +0.1 278,847 + +1.0 278,848 - +0.2 278,848 - +0.1 278,848 - +2.0 278,848 - +0.1 278,869 + -0.1 278,870 - -0.2 278,870 - -0.4 278,912 + +1.3 278,917 - -0.9 278,917 - -0.1 278,917 - -0.6 279,005 + -1.0 279,013 + +2.4 279,040 + -0.4 279,116 + +0.1 279,213 - -0.4 279,293 + EX31_RS01250 0.15 +0.0 279,294 - EX31_RS01250 0.15 -0.4 279,336 - EX31_RS01250 0.18 +1.1 279,336 - EX31_RS01250 0.18 +0.8 279,339 - EX31_RS01250 0.18 -0.2 279,388 + EX31_RS01250 0.21 -0.9 279,388 + EX31_RS01250 0.21 +1.1 279,452 - EX31_RS01250 0.26 -1.2 279,452 - EX31_RS01250 0.26 -0.4 279,452 - EX31_RS01250 0.26 +0.6 279,534 + EX31_RS01250 0.32 -0.2 279,547 + EX31_RS01250 0.33 +1.1 279,548 - EX31_RS01250 0.33 +0.5 279,548 - EX31_RS01250 0.33 -2.6 279,595 - EX31_RS01250 0.36 +0.6 279,619 + EX31_RS01250 0.38 +1.3 279,620 - EX31_RS01250 0.38 +1.1 279,671 + EX31_RS01250 0.42 +1.2 279,780 + EX31_RS01250 0.49 +0.5 279,893 + EX31_RS01250 0.58 +0.3 279,893 + EX31_RS01250 0.58 +1.1 279,894 - EX31_RS01250 0.58 -1.1 280,225 + EX31_RS01250 0.81 +1.2 280,225 + EX31_RS01250 0.81 +0.7 280,225 + EX31_RS01250 0.81 +0.4 280,225 + EX31_RS01250 0.81 +0.6 280,226 - EX31_RS01250 0.81 +0.0 280,226 - EX31_RS01250 0.81 +0.5 280,226 - EX31_RS01250 0.81 -0.5 280,226 - EX31_RS01250 0.81 +0.5 280,226 - EX31_RS01250 0.81 +0.3 280,226 - EX31_RS01250 0.81 +1.7 280,294 + EX31_RS01250 0.86 +0.3 280,327 + EX31_RS01250 0.89 -0.1 280,327 + EX31_RS01250 0.89 -0.2 280,328 - EX31_RS01250 0.89 +0.7 280,433 - -0.6 280,433 - +0.7 280,466 - +2.1 280,520 - -0.8 280,582 - -1.0 280,582 - +0.4 280,614 + +1.4 280,737 - EX31_RS01255 0.19 +1.0 280,784 - EX31_RS01255 0.26 -0.8 280,960 - EX31_RS01255 0.54 +1.5 281,007 - EX31_RS01255 0.61 +0.2 281,007 - EX31_RS01255 0.61 -0.2 281,120 + EX31_RS01255 0.79 +1.4 281,192 + -2.0 281,192 + -0.1 281,212 + -0.2 281,212 + +1.9 281,317 - +0.2 281,317 - -0.1
Or see this region's nucleotide sequence