Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00355 and EX31_RS00360 are separated by 100 nucleotides EX31_RS00360 and EX31_RS00365 are separated by 41 nucleotides
EX31_RS00355: EX31_RS00355 - mandelate racemase, at 74,349 to 75,503
_RS00355
EX31_RS00360: EX31_RS00360 - ABC transporter substrate-binding protein, at 75,604 to 77,199
_RS00360
EX31_RS00365: EX31_RS00365 - ABC transporter permease, at 77,241 to 78,257
_RS00365
Position (kb) 75
76
77
78 Strain fitness (log2 ratio) -2
-1
0
1
2 at 74.681 kb on + strand, within EX31_RS00355 at 74.926 kb on + strand, within EX31_RS00355 at 75.075 kb on + strand, within EX31_RS00355 at 75.075 kb on + strand, within EX31_RS00355 at 75.363 kb on + strand, within EX31_RS00355 at 75.381 kb on + strand, within EX31_RS00355 at 75.461 kb on + strand at 75.461 kb on + strand at 75.461 kb on + strand at 75.462 kb on - strand at 75.475 kb on + strand at 75.475 kb on + strand at 75.475 kb on + strand at 75.475 kb on + strand at 75.475 kb on + strand at 75.475 kb on + strand at 75.475 kb on + strand at 75.476 kb on - strand at 75.476 kb on - strand at 75.476 kb on - strand at 75.476 kb on - strand at 75.476 kb on - strand at 75.476 kb on - strand at 75.476 kb on - strand at 75.486 kb on - strand at 75.526 kb on - strand at 75.550 kb on + strand at 75.550 kb on + strand at 75.550 kb on + strand at 75.550 kb on + strand at 75.550 kb on + strand at 75.551 kb on - strand at 75.673 kb on - strand at 75.714 kb on + strand at 75.715 kb on - strand at 75.715 kb on - strand at 75.739 kb on + strand at 76.060 kb on + strand, within EX31_RS00360 at 76.060 kb on + strand, within EX31_RS00360 at 76.061 kb on - strand, within EX31_RS00360 at 76.237 kb on + strand, within EX31_RS00360 at 76.238 kb on - strand, within EX31_RS00360 at 76.238 kb on - strand, within EX31_RS00360 at 76.341 kb on + strand, within EX31_RS00360 at 76.341 kb on + strand, within EX31_RS00360 at 76.341 kb on + strand, within EX31_RS00360 at 76.420 kb on + strand, within EX31_RS00360 at 76.421 kb on - strand, within EX31_RS00360 at 76.421 kb on - strand, within EX31_RS00360 at 76.423 kb on + strand, within EX31_RS00360 at 76.486 kb on + strand, within EX31_RS00360 at 76.486 kb on + strand, within EX31_RS00360 at 76.487 kb on - strand, within EX31_RS00360 at 76.566 kb on + strand, within EX31_RS00360 at 76.596 kb on + strand, within EX31_RS00360 at 76.596 kb on + strand, within EX31_RS00360 at 76.597 kb on - strand, within EX31_RS00360 at 76.597 kb on - strand, within EX31_RS00360 at 76.597 kb on - strand, within EX31_RS00360 at 76.659 kb on + strand, within EX31_RS00360 at 76.662 kb on + strand, within EX31_RS00360 at 76.662 kb on + strand, within EX31_RS00360 at 76.662 kb on + strand, within EX31_RS00360 at 76.662 kb on + strand, within EX31_RS00360 at 76.663 kb on - strand, within EX31_RS00360 at 76.663 kb on - strand, within EX31_RS00360 at 76.698 kb on + strand, within EX31_RS00360 at 76.874 kb on - strand, within EX31_RS00360 at 76.937 kb on - strand, within EX31_RS00360 at 77.008 kb on + strand, within EX31_RS00360 at 77.029 kb on + strand, within EX31_RS00360 at 77.107 kb on + strand at 77.187 kb on + strand at 77.188 kb on - strand at 77.188 kb on - strand at 77.195 kb on + strand at 77.196 kb on - strand at 77.391 kb on + strand, within EX31_RS00365 at 77.391 kb on + strand, within EX31_RS00365 at 77.391 kb on + strand, within EX31_RS00365 at 77.391 kb on + strand, within EX31_RS00365 at 77.391 kb on + strand, within EX31_RS00365 at 77.391 kb on + strand, within EX31_RS00365 at 77.391 kb on + strand, within EX31_RS00365 at 77.391 kb on + strand, within EX31_RS00365 at 77.391 kb on + strand, within EX31_RS00365 at 77.391 kb on + strand, within EX31_RS00365 at 77.391 kb on + strand, within EX31_RS00365 at 77.391 kb on + strand, within EX31_RS00365 at 77.392 kb on - strand, within EX31_RS00365 at 77.392 kb on - strand, within EX31_RS00365 at 77.392 kb on - strand, within EX31_RS00365 at 77.403 kb on + strand, within EX31_RS00365 at 77.404 kb on - strand, within EX31_RS00365 at 77.448 kb on - strand, within EX31_RS00365 at 77.451 kb on + strand, within EX31_RS00365 at 77.451 kb on + strand, within EX31_RS00365 at 77.637 kb on + strand, within EX31_RS00365 at 77.638 kb on - strand, within EX31_RS00365 at 77.638 kb on - strand, within EX31_RS00365 at 77.668 kb on - strand, within EX31_RS00365 at 77.716 kb on - strand, within EX31_RS00365 at 77.795 kb on + strand, within EX31_RS00365 at 77.795 kb on + strand, within EX31_RS00365 at 77.898 kb on + strand, within EX31_RS00365 at 77.924 kb on + strand, within EX31_RS00365 at 77.924 kb on + strand, within EX31_RS00365 at 77.924 kb on + strand, within EX31_RS00365 at 77.951 kb on + strand, within EX31_RS00365 at 78.054 kb on + strand, within EX31_RS00365 at 78.121 kb on - strand, within EX31_RS00365 at 78.137 kb on + strand, within EX31_RS00365 at 78.138 kb on - strand, within EX31_RS00365 at 78.185 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 74,681 + EX31_RS00355 0.29 +0.2 74,926 + EX31_RS00355 0.50 -0.3 75,075 + EX31_RS00355 0.63 -0.8 75,075 + EX31_RS00355 0.63 -0.1 75,363 + EX31_RS00355 0.88 -1.7 75,381 + EX31_RS00355 0.89 +1.1 75,461 + +0.6 75,461 + +0.8 75,461 + -1.3 75,462 - +0.3 75,475 + -0.6 75,475 + -0.3 75,475 + -0.0 75,475 + +0.4 75,475 + +0.1 75,475 + -0.5 75,475 + +1.2 75,476 - +0.7 75,476 - -0.5 75,476 - +0.1 75,476 - +0.5 75,476 - +1.2 75,476 - +0.3 75,476 - +0.2 75,486 - -1.3 75,526 - -0.2 75,550 + +1.0 75,550 + -0.2 75,550 + +0.6 75,550 + +0.9 75,550 + -0.0 75,551 - +0.2 75,673 - -1.5 75,714 + +0.3 75,715 - -0.5 75,715 - +0.5 75,739 + +0.6 76,060 + EX31_RS00360 0.29 -0.9 76,060 + EX31_RS00360 0.29 -0.8 76,061 - EX31_RS00360 0.29 -0.5 76,237 + EX31_RS00360 0.40 -0.1 76,238 - EX31_RS00360 0.40 -0.4 76,238 - EX31_RS00360 0.40 -2.5 76,341 + EX31_RS00360 0.46 -0.5 76,341 + EX31_RS00360 0.46 +0.5 76,341 + EX31_RS00360 0.46 -0.3 76,420 + EX31_RS00360 0.51 +2.0 76,421 - EX31_RS00360 0.51 +0.2 76,421 - EX31_RS00360 0.51 +1.4 76,423 + EX31_RS00360 0.51 -0.1 76,486 + EX31_RS00360 0.55 -0.3 76,486 + EX31_RS00360 0.55 -2.5 76,487 - EX31_RS00360 0.55 +2.6 76,566 + EX31_RS00360 0.60 -0.3 76,596 + EX31_RS00360 0.62 -0.5 76,596 + EX31_RS00360 0.62 -0.8 76,597 - EX31_RS00360 0.62 -1.0 76,597 - EX31_RS00360 0.62 +0.3 76,597 - EX31_RS00360 0.62 -0.3 76,659 + EX31_RS00360 0.66 -0.5 76,662 + EX31_RS00360 0.66 -0.3 76,662 + EX31_RS00360 0.66 -0.1 76,662 + EX31_RS00360 0.66 -0.1 76,662 + EX31_RS00360 0.66 +0.9 76,663 - EX31_RS00360 0.66 +0.0 76,663 - EX31_RS00360 0.66 +0.3 76,698 + EX31_RS00360 0.69 -1.3 76,874 - EX31_RS00360 0.80 -1.9 76,937 - EX31_RS00360 0.84 -1.0 77,008 + EX31_RS00360 0.88 -1.3 77,029 + EX31_RS00360 0.89 -1.3 77,107 + -1.0 77,187 + +0.5 77,188 - -0.9 77,188 - -0.0 77,195 + -1.3 77,196 - +0.6 77,391 + EX31_RS00365 0.15 +0.2 77,391 + EX31_RS00365 0.15 +0.5 77,391 + EX31_RS00365 0.15 +0.2 77,391 + EX31_RS00365 0.15 -0.4 77,391 + EX31_RS00365 0.15 +0.0 77,391 + EX31_RS00365 0.15 -2.6 77,391 + EX31_RS00365 0.15 +0.3 77,391 + EX31_RS00365 0.15 -1.1 77,391 + EX31_RS00365 0.15 +0.2 77,391 + EX31_RS00365 0.15 -0.6 77,391 + EX31_RS00365 0.15 +0.4 77,391 + EX31_RS00365 0.15 -0.5 77,392 - EX31_RS00365 0.15 +0.8 77,392 - EX31_RS00365 0.15 +0.1 77,392 - EX31_RS00365 0.15 +1.0 77,403 + EX31_RS00365 0.16 -1.0 77,404 - EX31_RS00365 0.16 +1.3 77,448 - EX31_RS00365 0.20 +1.2 77,451 + EX31_RS00365 0.21 -0.9 77,451 + EX31_RS00365 0.21 +0.7 77,637 + EX31_RS00365 0.39 +0.5 77,638 - EX31_RS00365 0.39 -1.0 77,638 - EX31_RS00365 0.39 +0.7 77,668 - EX31_RS00365 0.42 -0.2 77,716 - EX31_RS00365 0.47 -0.0 77,795 + EX31_RS00365 0.54 +1.2 77,795 + EX31_RS00365 0.54 +1.5 77,898 + EX31_RS00365 0.65 -1.2 77,924 + EX31_RS00365 0.67 +1.0 77,924 + EX31_RS00365 0.67 +1.3 77,924 + EX31_RS00365 0.67 -1.0 77,951 + EX31_RS00365 0.70 -1.0 78,054 + EX31_RS00365 0.80 +1.6 78,121 - EX31_RS00365 0.87 +0.5 78,137 + EX31_RS00365 0.88 -1.0 78,138 - EX31_RS00365 0.88 +1.8 78,185 + +0.3
Or see this region's nucleotide sequence