Strain Fitness in Rahnella sp. WP5 around EX31_RS00260

Experiment: L-Glutamine (N); RCH2 media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS00250 and EX31_RS00255 are separated by 71 nucleotidesEX31_RS00255 and EX31_RS00260 are separated by 141 nucleotidesEX31_RS00260 and EX31_RS00265 are separated by 21 nucleotides EX31_RS00250: EX31_RS00250 - aldehyde dehydrogenase, at 51,730 to 53,169 _RS00250 EX31_RS00255: EX31_RS00255 - hypothetical protein, at 53,241 to 53,822 _RS00255 EX31_RS00260: EX31_RS00260 - hypothetical protein, at 53,964 to 55,040 _RS00260 EX31_RS00265: EX31_RS00265 - alpha/beta hydrolase, at 55,062 to 55,820 _RS00265 Position (kb) 53 54 55 56Strain fitness (log2 ratio) -2 -1 0 1 2at 52.972 kb on + strand, within EX31_RS00250at 52.972 kb on + strand, within EX31_RS00250at 52.973 kb on - strand, within EX31_RS00250at 52.983 kb on + strand, within EX31_RS00250at 52.984 kb on - strand, within EX31_RS00250at 52.984 kb on - strand, within EX31_RS00250at 52.985 kb on + strand, within EX31_RS00250at 52.986 kb on - strand, within EX31_RS00250at 52.986 kb on - strand, within EX31_RS00250at 52.986 kb on - strand, within EX31_RS00250at 53.032 kb on + strandat 53.032 kb on + strandat 53.085 kb on + strandat 53.131 kb on + strandat 53.168 kb on - strandat 53.171 kb on + strandat 53.172 kb on - strandat 53.280 kb on - strandat 53.424 kb on + strand, within EX31_RS00255at 53.425 kb on - strand, within EX31_RS00255at 53.437 kb on + strand, within EX31_RS00255at 53.437 kb on + strand, within EX31_RS00255at 53.438 kb on - strand, within EX31_RS00255at 53.438 kb on - strand, within EX31_RS00255at 53.438 kb on - strand, within EX31_RS00255at 53.438 kb on - strand, within EX31_RS00255at 53.439 kb on + strand, within EX31_RS00255at 53.440 kb on - strand, within EX31_RS00255at 53.440 kb on - strand, within EX31_RS00255at 53.481 kb on - strand, within EX31_RS00255at 53.722 kb on - strand, within EX31_RS00255at 53.729 kb on + strand, within EX31_RS00255at 53.730 kb on - strand, within EX31_RS00255at 53.730 kb on - strand, within EX31_RS00255at 53.730 kb on - strand, within EX31_RS00255at 53.820 kb on + strandat 53.821 kb on - strandat 53.862 kb on - strandat 53.875 kb on + strandat 53.875 kb on + strandat 53.884 kb on + strandat 53.891 kb on + strandat 53.974 kb on + strandat 54.068 kb on + strandat 54.069 kb on - strandat 54.137 kb on + strand, within EX31_RS00260at 54.137 kb on + strand, within EX31_RS00260at 54.159 kb on + strand, within EX31_RS00260at 54.161 kb on + strand, within EX31_RS00260at 54.161 kb on + strand, within EX31_RS00260at 54.162 kb on - strand, within EX31_RS00260at 54.213 kb on + strand, within EX31_RS00260at 54.213 kb on + strand, within EX31_RS00260at 54.213 kb on + strand, within EX31_RS00260at 54.267 kb on - strand, within EX31_RS00260at 54.308 kb on + strand, within EX31_RS00260at 54.308 kb on + strand, within EX31_RS00260at 54.309 kb on - strand, within EX31_RS00260at 54.346 kb on + strand, within EX31_RS00260at 54.346 kb on + strand, within EX31_RS00260at 54.453 kb on + strand, within EX31_RS00260at 54.560 kb on + strand, within EX31_RS00260at 54.597 kb on + strand, within EX31_RS00260at 54.597 kb on + strand, within EX31_RS00260at 54.597 kb on + strand, within EX31_RS00260at 54.598 kb on - strand, within EX31_RS00260at 54.598 kb on - strand, within EX31_RS00260at 54.658 kb on + strand, within EX31_RS00260at 54.684 kb on + strand, within EX31_RS00260at 54.685 kb on - strand, within EX31_RS00260at 54.685 kb on - strand, within EX31_RS00260at 54.685 kb on - strand, within EX31_RS00260at 54.685 kb on - strand, within EX31_RS00260at 54.809 kb on + strand, within EX31_RS00260at 54.810 kb on - strand, within EX31_RS00260at 54.886 kb on + strand, within EX31_RS00260at 54.886 kb on + strand, within EX31_RS00260at 54.887 kb on - strand, within EX31_RS00260at 54.925 kb on + strand, within EX31_RS00260at 54.931 kb on - strand, within EX31_RS00260at 54.979 kb on + strandat 54.979 kb on + strandat 54.999 kb on + strandat 54.999 kb on + strandat 54.999 kb on + strandat 55.063 kb on + strandat 55.063 kb on + strandat 55.064 kb on - strandat 55.064 kb on - strandat 55.064 kb on - strandat 55.127 kb on - strandat 55.183 kb on + strand, within EX31_RS00265at 55.184 kb on - strand, within EX31_RS00265at 55.184 kb on - strand, within EX31_RS00265at 55.184 kb on - strand, within EX31_RS00265at 55.194 kb on + strand, within EX31_RS00265at 55.195 kb on - strand, within EX31_RS00265at 55.195 kb on - strand, within EX31_RS00265at 55.234 kb on + strand, within EX31_RS00265at 55.234 kb on + strand, within EX31_RS00265at 55.235 kb on - strand, within EX31_RS00265at 55.280 kb on + strand, within EX31_RS00265at 55.280 kb on + strand, within EX31_RS00265at 55.281 kb on - strand, within EX31_RS00265at 55.281 kb on - strand, within EX31_RS00265at 55.284 kb on + strand, within EX31_RS00265at 55.284 kb on + strand, within EX31_RS00265at 55.334 kb on + strand, within EX31_RS00265at 55.334 kb on + strand, within EX31_RS00265at 55.334 kb on + strand, within EX31_RS00265at 55.384 kb on + strand, within EX31_RS00265at 55.384 kb on + strand, within EX31_RS00265at 55.489 kb on + strand, within EX31_RS00265at 55.489 kb on + strand, within EX31_RS00265at 55.490 kb on - strand, within EX31_RS00265at 55.490 kb on - strand, within EX31_RS00265at 55.490 kb on - strand, within EX31_RS00265at 55.490 kb on - strand, within EX31_RS00265at 55.490 kb on - strand, within EX31_RS00265at 55.490 kb on - strand, within EX31_RS00265at 55.490 kb on - strand, within EX31_RS00265at 55.490 kb on - strand, within EX31_RS00265at 55.490 kb on - strand, within EX31_RS00265at 55.533 kb on - strand, within EX31_RS00265at 55.546 kb on + strand, within EX31_RS00265at 55.598 kb on + strand, within EX31_RS00265at 55.641 kb on + strand, within EX31_RS00265at 55.642 kb on - strand, within EX31_RS00265at 55.655 kb on + strand, within EX31_RS00265at 55.655 kb on + strand, within EX31_RS00265at 55.658 kb on - strand, within EX31_RS00265at 55.681 kb on + strand, within EX31_RS00265at 55.682 kb on - strand, within EX31_RS00265at 55.682 kb on - strand, within EX31_RS00265at 55.684 kb on - strand, within EX31_RS00265at 55.733 kb on + strand, within EX31_RS00265at 55.733 kb on + strand, within EX31_RS00265at 55.806 kb on + strandat 55.807 kb on - strandat 55.812 kb on + strandat 55.812 kb on + strandat 55.813 kb on - strandat 55.813 kb on - strandat 55.813 kb on - strandat 55.836 kb on - strandat 55.836 kb on - strandat 55.986 kb on + strandat 56.012 kb on - strandat 56.022 kb on + strandat 56.023 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking
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52,972 + EX31_RS00250 0.86 -0.3
52,972 + EX31_RS00250 0.86 +0.6
52,973 - EX31_RS00250 0.86 -0.1
52,983 + EX31_RS00250 0.87 -0.0
52,984 - EX31_RS00250 0.87 +0.5
52,984 - EX31_RS00250 0.87 +0.9
52,985 + EX31_RS00250 0.87 +1.7
52,986 - EX31_RS00250 0.87 +0.1
52,986 - EX31_RS00250 0.87 -0.4
52,986 - EX31_RS00250 0.87 +0.4
53,032 + +1.0
53,032 + -0.2
53,085 + +1.0
53,131 + -0.5
53,168 - +0.9
53,171 + -0.7
53,172 - +0.0
53,280 - +0.7
53,424 + EX31_RS00255 0.31 -1.5
53,425 - EX31_RS00255 0.32 -0.5
53,437 + EX31_RS00255 0.34 +0.8
53,437 + EX31_RS00255 0.34 +1.0
53,438 - EX31_RS00255 0.34 +0.4
53,438 - EX31_RS00255 0.34 +0.4
53,438 - EX31_RS00255 0.34 +1.6
53,438 - EX31_RS00255 0.34 +0.2
53,439 + EX31_RS00255 0.34 +0.5
53,440 - EX31_RS00255 0.34 -0.7
53,440 - EX31_RS00255 0.34 -1.1
53,481 - EX31_RS00255 0.41 +0.1
53,722 - EX31_RS00255 0.83 -0.4
53,729 + EX31_RS00255 0.84 +0.5
53,730 - EX31_RS00255 0.84 +0.8
53,730 - EX31_RS00255 0.84 +0.2
53,730 - EX31_RS00255 0.84 +1.2
53,820 + +1.8
53,821 - -0.9
53,862 - +0.0
53,875 + +1.6
53,875 + -0.5
53,884 + +1.0
53,891 + +0.9
53,974 + +0.5
54,068 + -0.3
54,069 - +0.3
54,137 + EX31_RS00260 0.16 -1.4
54,137 + EX31_RS00260 0.16 +0.6
54,159 + EX31_RS00260 0.18 -0.2
54,161 + EX31_RS00260 0.18 -1.7
54,161 + EX31_RS00260 0.18 -0.7
54,162 - EX31_RS00260 0.18 -0.1
54,213 + EX31_RS00260 0.23 +0.5
54,213 + EX31_RS00260 0.23 +1.0
54,213 + EX31_RS00260 0.23 -0.9
54,267 - EX31_RS00260 0.28 +0.6
54,308 + EX31_RS00260 0.32 +1.9
54,308 + EX31_RS00260 0.32 +0.1
54,309 - EX31_RS00260 0.32 -2.3
54,346 + EX31_RS00260 0.35 -0.4
54,346 + EX31_RS00260 0.35 +0.0
54,453 + EX31_RS00260 0.45 +0.0
54,560 + EX31_RS00260 0.55 -0.5
54,597 + EX31_RS00260 0.59 -0.5
54,597 + EX31_RS00260 0.59 -0.5
54,597 + EX31_RS00260 0.59 +0.4
54,598 - EX31_RS00260 0.59 +0.8
54,598 - EX31_RS00260 0.59 +1.1
54,658 + EX31_RS00260 0.64 +0.6
54,684 + EX31_RS00260 0.67 +0.6
54,685 - EX31_RS00260 0.67 -1.1
54,685 - EX31_RS00260 0.67 +1.4
54,685 - EX31_RS00260 0.67 +0.3
54,685 - EX31_RS00260 0.67 +0.8
54,809 + EX31_RS00260 0.78 -0.3
54,810 - EX31_RS00260 0.79 +0.7
54,886 + EX31_RS00260 0.86 -2.6
54,886 + EX31_RS00260 0.86 -1.3
54,887 - EX31_RS00260 0.86 +0.6
54,925 + EX31_RS00260 0.89 +0.0
54,931 - EX31_RS00260 0.90 +0.5
54,979 + -0.5
54,979 + +0.2
54,999 + +0.9
54,999 + -0.5
54,999 + -0.4
55,063 + -0.3
55,063 + +0.8
55,064 - -1.2
55,064 - -0.3
55,064 - -1.2
55,127 - +0.2
55,183 + EX31_RS00265 0.16 +0.7
55,184 - EX31_RS00265 0.16 -0.0
55,184 - EX31_RS00265 0.16 +1.3
55,184 - EX31_RS00265 0.16 +0.5
55,194 + EX31_RS00265 0.17 -0.8
55,195 - EX31_RS00265 0.18 +0.9
55,195 - EX31_RS00265 0.18 -1.1
55,234 + EX31_RS00265 0.23 +0.5
55,234 + EX31_RS00265 0.23 +0.8
55,235 - EX31_RS00265 0.23 -1.6
55,280 + EX31_RS00265 0.29 -0.7
55,280 + EX31_RS00265 0.29 +0.5
55,281 - EX31_RS00265 0.29 +0.2
55,281 - EX31_RS00265 0.29 +0.0
55,284 + EX31_RS00265 0.29 -0.5
55,284 + EX31_RS00265 0.29 -0.8
55,334 + EX31_RS00265 0.36 -0.3
55,334 + EX31_RS00265 0.36 +0.8
55,334 + EX31_RS00265 0.36 +0.7
55,384 + EX31_RS00265 0.42 -0.9
55,384 + EX31_RS00265 0.42 -0.4
55,489 + EX31_RS00265 0.56 -1.0
55,489 + EX31_RS00265 0.56 +2.5
55,490 - EX31_RS00265 0.56 -0.2
55,490 - EX31_RS00265 0.56 -1.2
55,490 - EX31_RS00265 0.56 +0.5
55,490 - EX31_RS00265 0.56 +0.9
55,490 - EX31_RS00265 0.56 +1.5
55,490 - EX31_RS00265 0.56 +0.5
55,490 - EX31_RS00265 0.56 -0.0
55,490 - EX31_RS00265 0.56 -0.7
55,490 - EX31_RS00265 0.56 +0.1
55,533 - EX31_RS00265 0.62 +1.1
55,546 + EX31_RS00265 0.64 +0.4
55,598 + EX31_RS00265 0.71 +0.3
55,641 + EX31_RS00265 0.76 +0.5
55,642 - EX31_RS00265 0.76 +0.2
55,655 + EX31_RS00265 0.78 +1.3
55,655 + EX31_RS00265 0.78 -0.8
55,658 - EX31_RS00265 0.79 +0.9
55,681 + EX31_RS00265 0.82 -1.6
55,682 - EX31_RS00265 0.82 -0.5
55,682 - EX31_RS00265 0.82 +0.0
55,684 - EX31_RS00265 0.82 -0.4
55,733 + EX31_RS00265 0.88 -0.1
55,733 + EX31_RS00265 0.88 +0.8
55,806 + +2.0
55,807 - -0.5
55,812 + -0.5
55,812 + +0.8
55,813 - -0.8
55,813 - +0.5
55,813 - -0.7
55,836 - +0.1
55,836 - +2.0
55,986 + -0.2
56,012 - +0.9
56,022 + -1.3
56,023 - -0.0

Or see this region's nucleotide sequence