Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00240 and EX31_RS00245 are separated by 401 nucleotides EX31_RS00245 and EX31_RS00250 are separated by 195 nucleotides
EX31_RS00240: EX31_RS00240 - rhamnose ABC transporter substrate-binding protein, at 49,316 to 50,308
_RS00240
EX31_RS00245: EX31_RS00245 - HTH-type transcriptional activator RhaS, at 50,710 to 51,534
_RS00245
EX31_RS00250: EX31_RS00250 - aldehyde dehydrogenase, at 51,730 to 53,169
_RS00250
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 49.839 kb on - strand, within EX31_RS00240 at 49.839 kb on - strand, within EX31_RS00240 at 49.927 kb on + strand, within EX31_RS00240 at 49.927 kb on + strand, within EX31_RS00240 at 49.928 kb on - strand, within EX31_RS00240 at 49.981 kb on + strand, within EX31_RS00240 at 49.982 kb on - strand, within EX31_RS00240 at 50.090 kb on + strand, within EX31_RS00240 at 50.091 kb on - strand, within EX31_RS00240 at 50.128 kb on - strand, within EX31_RS00240 at 50.128 kb on - strand, within EX31_RS00240 at 50.156 kb on - strand, within EX31_RS00240 at 50.201 kb on - strand, within EX31_RS00240 at 50.292 kb on - strand at 50.301 kb on - strand at 50.315 kb on + strand at 50.324 kb on + strand at 50.324 kb on + strand at 50.387 kb on + strand at 50.387 kb on + strand at 50.387 kb on + strand at 50.440 kb on - strand at 50.557 kb on + strand at 50.564 kb on + strand at 50.564 kb on + strand at 50.675 kb on + strand at 50.675 kb on + strand at 50.680 kb on + strand at 50.680 kb on + strand at 50.683 kb on + strand at 50.689 kb on - strand at 50.727 kb on + strand at 50.968 kb on + strand, within EX31_RS00245 at 51.096 kb on + strand, within EX31_RS00245 at 51.096 kb on + strand, within EX31_RS00245 at 51.155 kb on + strand, within EX31_RS00245 at 51.156 kb on - strand, within EX31_RS00245 at 51.156 kb on - strand, within EX31_RS00245 at 51.158 kb on - strand, within EX31_RS00245 at 51.162 kb on + strand, within EX31_RS00245 at 51.173 kb on + strand, within EX31_RS00245 at 51.173 kb on + strand, within EX31_RS00245 at 51.174 kb on - strand, within EX31_RS00245 at 51.176 kb on + strand, within EX31_RS00245 at 51.262 kb on + strand, within EX31_RS00245 at 51.263 kb on - strand, within EX31_RS00245 at 51.263 kb on - strand, within EX31_RS00245 at 51.469 kb on + strand at 51.469 kb on + strand at 51.469 kb on + strand at 51.469 kb on + strand at 51.470 kb on - strand at 51.526 kb on + strand at 51.547 kb on - strand at 51.547 kb on - strand at 51.562 kb on + strand at 51.562 kb on + strand at 51.607 kb on + strand at 51.608 kb on - strand at 51.608 kb on - strand at 51.608 kb on - strand at 51.713 kb on - strand at 51.727 kb on + strand at 51.727 kb on + strand at 51.727 kb on + strand at 51.727 kb on + strand at 51.728 kb on - strand at 51.728 kb on - strand at 51.728 kb on - strand at 51.728 kb on - strand at 51.728 kb on - strand at 51.728 kb on - strand at 51.750 kb on + strand at 51.750 kb on + strand at 51.750 kb on + strand at 51.750 kb on + strand at 51.750 kb on + strand at 51.750 kb on + strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.751 kb on - strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.754 kb on + strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.755 kb on - strand at 51.945 kb on + strand, within EX31_RS00250 at 52.019 kb on - strand, within EX31_RS00250 at 52.041 kb on - strand, within EX31_RS00250 at 52.041 kb on - strand, within EX31_RS00250 at 52.058 kb on - strand, within EX31_RS00250 at 52.058 kb on - strand, within EX31_RS00250 at 52.066 kb on + strand, within EX31_RS00250 at 52.066 kb on + strand, within EX31_RS00250 at 52.066 kb on + strand, within EX31_RS00250 at 52.066 kb on + strand, within EX31_RS00250 at 52.066 kb on + strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.067 kb on - strand, within EX31_RS00250 at 52.239 kb on + strand, within EX31_RS00250 at 52.240 kb on - strand, within EX31_RS00250 at 52.240 kb on - strand, within EX31_RS00250 at 52.300 kb on - strand, within EX31_RS00250 at 52.335 kb on + strand, within EX31_RS00250 at 52.386 kb on + strand, within EX31_RS00250
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 49,839 - EX31_RS00240 0.53 +2.0 49,839 - EX31_RS00240 0.53 -0.4 49,927 + EX31_RS00240 0.62 -0.1 49,927 + EX31_RS00240 0.62 -0.2 49,928 - EX31_RS00240 0.62 +0.6 49,981 + EX31_RS00240 0.67 +0.0 49,982 - EX31_RS00240 0.67 -0.3 50,090 + EX31_RS00240 0.78 -0.4 50,091 - EX31_RS00240 0.78 -0.7 50,128 - EX31_RS00240 0.82 -0.1 50,128 - EX31_RS00240 0.82 -1.9 50,156 - EX31_RS00240 0.85 +0.2 50,201 - EX31_RS00240 0.89 +1.5 50,292 - +0.3 50,301 - -0.5 50,315 + -0.3 50,324 + +0.8 50,324 + -0.3 50,387 + +1.6 50,387 + +0.5 50,387 + +1.2 50,440 - +0.5 50,557 + -0.3 50,564 + +1.5 50,564 + -0.2 50,675 + -0.1 50,675 + +0.2 50,680 + -1.3 50,680 + +0.0 50,683 + -0.0 50,689 - +0.6 50,727 + +0.8 50,968 + EX31_RS00245 0.31 +0.6 51,096 + EX31_RS00245 0.47 +0.3 51,096 + EX31_RS00245 0.47 +0.6 51,155 + EX31_RS00245 0.54 -0.1 51,156 - EX31_RS00245 0.54 +1.3 51,156 - EX31_RS00245 0.54 +0.8 51,158 - EX31_RS00245 0.54 +0.4 51,162 + EX31_RS00245 0.55 +0.3 51,173 + EX31_RS00245 0.56 +1.6 51,173 + EX31_RS00245 0.56 -1.0 51,174 - EX31_RS00245 0.56 -0.3 51,176 + EX31_RS00245 0.56 +1.0 51,262 + EX31_RS00245 0.67 -0.7 51,263 - EX31_RS00245 0.67 +1.0 51,263 - EX31_RS00245 0.67 +1.3 51,469 + +1.0 51,469 + +1.0 51,469 + +0.7 51,469 + -1.1 51,470 - +0.9 51,526 + +0.0 51,547 - +1.4 51,547 - +0.9 51,562 + +0.0 51,562 + -0.2 51,607 + +0.6 51,608 - -0.0 51,608 - +0.6 51,608 - +0.2 51,713 - +1.9 51,727 + -1.1 51,727 + +1.0 51,727 + +2.0 51,727 + -0.4 51,728 - +1.0 51,728 - -1.1 51,728 - +1.5 51,728 - +0.5 51,728 - -0.8 51,728 - +2.4 51,750 + -0.5 51,750 + +1.0 51,750 + -0.8 51,750 + -1.1 51,750 + +0.7 51,750 + -1.0 51,751 - -1.3 51,751 - +0.9 51,751 - -0.5 51,751 - +3.6 51,751 - -0.3 51,751 - -0.5 51,751 - +4.0 51,751 - -0.3 51,751 - -0.7 51,751 - +0.1 51,754 + -0.3 51,754 + +0.7 51,754 + -1.1 51,754 + +1.2 51,754 + +0.8 51,754 + +0.2 51,754 + +1.4 51,754 + -0.7 51,754 + +2.0 51,754 + +1.2 51,754 + -0.5 51,754 + +0.8 51,755 - -0.1 51,755 - -1.0 51,755 - +0.6 51,755 - +1.4 51,755 - +0.2 51,755 - +1.0 51,755 - +0.5 51,945 + EX31_RS00250 0.15 +0.8 52,019 - EX31_RS00250 0.20 -0.1 52,041 - EX31_RS00250 0.22 +0.7 52,041 - EX31_RS00250 0.22 -0.3 52,058 - EX31_RS00250 0.23 +0.7 52,058 - EX31_RS00250 0.23 -0.8 52,066 + EX31_RS00250 0.23 +0.7 52,066 + EX31_RS00250 0.23 -0.5 52,066 + EX31_RS00250 0.23 -1.0 52,066 + EX31_RS00250 0.23 -0.4 52,066 + EX31_RS00250 0.23 +0.1 52,067 - EX31_RS00250 0.23 +0.2 52,067 - EX31_RS00250 0.23 +1.0 52,067 - EX31_RS00250 0.23 +0.2 52,067 - EX31_RS00250 0.23 +1.2 52,067 - EX31_RS00250 0.23 -1.0 52,067 - EX31_RS00250 0.23 -0.3 52,067 - EX31_RS00250 0.23 +1.2 52,067 - EX31_RS00250 0.23 +1.1 52,067 - EX31_RS00250 0.23 +0.2 52,067 - EX31_RS00250 0.23 -0.3 52,239 + EX31_RS00250 0.35 +0.7 52,240 - EX31_RS00250 0.35 -0.1 52,240 - EX31_RS00250 0.35 +1.2 52,300 - EX31_RS00250 0.40 -0.5 52,335 + EX31_RS00250 0.42 -1.3 52,386 + EX31_RS00250 0.46 +0.5
Or see this region's nucleotide sequence