Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04220 and MMP_RS04225 are separated by 86 nucleotides MMP_RS04225 and MMP_RS04230 are separated by 80 nucleotides MMP_RS04230 and MMP_RS04235 are separated by 334 nucleotides
MMP_RS04220: MMP_RS04220 - queuosine precursor transporter, at 802,414 to 803,022
_RS04220
MMP_RS04225: MMP_RS04225 - hypothetical protein, at 803,109 to 803,621
_RS04225
MMP_RS04230: MMP_RS04230 - hypothetical protein, at 803,702 to 805,855
_RS04230
MMP_RS04235: MMP_RS04235 - hypothetical protein, at 806,190 to 807,299
_RS04235
Position (kb) 803
804
805
806 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 802.723 kb on - strand, within MMP_RS04220 at 802.748 kb on - strand, within MMP_RS04220 at 802.869 kb on - strand, within MMP_RS04220 at 802.879 kb on - strand, within MMP_RS04220 at 802.880 kb on + strand, within MMP_RS04220 at 802.883 kb on - strand, within MMP_RS04220 at 802.971 kb on - strand at 802.971 kb on - strand at 802.971 kb on - strand at 802.971 kb on - strand at 802.971 kb on - strand at 802.972 kb on + strand at 802.972 kb on + strand at 803.016 kb on - strand at 803.032 kb on - strand at 803.033 kb on + strand at 803.036 kb on - strand at 803.036 kb on - strand at 803.037 kb on + strand at 803.046 kb on + strand at 803.081 kb on + strand at 803.126 kb on + strand at 803.130 kb on - strand at 803.151 kb on - strand at 803.152 kb on + strand at 803.152 kb on + strand at 803.164 kb on + strand, within MMP_RS04225 at 803.186 kb on - strand, within MMP_RS04225 at 803.196 kb on + strand, within MMP_RS04225 at 803.264 kb on - strand, within MMP_RS04225 at 803.265 kb on + strand, within MMP_RS04225 at 803.359 kb on - strand, within MMP_RS04225 at 803.405 kb on - strand, within MMP_RS04225 at 803.405 kb on - strand, within MMP_RS04225 at 803.476 kb on + strand, within MMP_RS04225 at 803.477 kb on - strand, within MMP_RS04225 at 803.622 kb on - strand at 803.622 kb on - strand at 803.623 kb on + strand at 803.623 kb on + strand at 803.624 kb on - strand at 803.624 kb on - strand at 803.624 kb on - strand at 803.643 kb on - strand at 803.643 kb on - strand at 803.659 kb on + strand at 803.663 kb on + strand at 803.663 kb on + strand at 803.663 kb on + strand at 803.663 kb on + strand at 803.664 kb on - strand at 803.694 kb on - strand at 803.694 kb on - strand at 803.695 kb on + strand at 803.703 kb on - strand at 803.714 kb on - strand at 803.714 kb on - strand at 803.715 kb on + strand at 803.715 kb on + strand at 803.715 kb on + strand at 803.826 kb on - strand at 803.827 kb on + strand at 803.852 kb on + strand at 803.865 kb on + strand at 803.921 kb on + strand, within MMP_RS04230 at 803.921 kb on + strand, within MMP_RS04230 at 803.946 kb on - strand, within MMP_RS04230 at 803.946 kb on - strand, within MMP_RS04230 at 803.946 kb on - strand, within MMP_RS04230 at 803.947 kb on + strand, within MMP_RS04230 at 804.080 kb on + strand, within MMP_RS04230 at 804.157 kb on - strand, within MMP_RS04230 at 804.173 kb on + strand, within MMP_RS04230 at 804.198 kb on - strand, within MMP_RS04230 at 804.200 kb on - strand, within MMP_RS04230 at 804.249 kb on + strand, within MMP_RS04230 at 804.296 kb on + strand, within MMP_RS04230 at 804.298 kb on - strand, within MMP_RS04230 at 804.424 kb on - strand, within MMP_RS04230 at 804.424 kb on - strand, within MMP_RS04230 at 804.515 kb on - strand, within MMP_RS04230 at 804.548 kb on - strand, within MMP_RS04230 at 804.607 kb on + strand, within MMP_RS04230 at 804.607 kb on + strand, within MMP_RS04230 at 804.625 kb on - strand, within MMP_RS04230 at 804.723 kb on - strand, within MMP_RS04230 at 804.725 kb on - strand, within MMP_RS04230 at 804.725 kb on - strand, within MMP_RS04230 at 804.725 kb on - strand, within MMP_RS04230 at 804.726 kb on + strand, within MMP_RS04230 at 804.726 kb on + strand, within MMP_RS04230 at 804.773 kb on - strand, within MMP_RS04230 at 804.774 kb on + strand, within MMP_RS04230 at 804.776 kb on + strand, within MMP_RS04230 at 804.826 kb on - strand, within MMP_RS04230 at 804.827 kb on + strand, within MMP_RS04230 at 804.827 kb on + strand, within MMP_RS04230 at 804.850 kb on - strand, within MMP_RS04230 at 804.850 kb on - strand, within MMP_RS04230 at 804.851 kb on + strand, within MMP_RS04230 at 804.851 kb on + strand, within MMP_RS04230 at 804.854 kb on + strand, within MMP_RS04230 at 804.854 kb on + strand, within MMP_RS04230 at 804.854 kb on + strand, within MMP_RS04230 at 804.855 kb on - strand, within MMP_RS04230 at 804.855 kb on - strand, within MMP_RS04230 at 804.855 kb on - strand, within MMP_RS04230 at 804.855 kb on - strand, within MMP_RS04230 at 804.855 kb on - strand, within MMP_RS04230 at 804.855 kb on - strand, within MMP_RS04230 at 804.856 kb on + strand, within MMP_RS04230 at 804.856 kb on + strand, within MMP_RS04230 at 804.856 kb on + strand, within MMP_RS04230 at 804.856 kb on + strand, within MMP_RS04230 at 804.869 kb on - strand, within MMP_RS04230 at 804.874 kb on - strand, within MMP_RS04230 at 804.874 kb on - strand, within MMP_RS04230 at 804.875 kb on + strand, within MMP_RS04230 at 804.887 kb on + strand, within MMP_RS04230 at 804.888 kb on - strand, within MMP_RS04230 at 804.910 kb on - strand, within MMP_RS04230 at 804.912 kb on - strand, within MMP_RS04230 at 804.912 kb on - strand, within MMP_RS04230 at 804.912 kb on - strand, within MMP_RS04230 at 804.912 kb on - strand, within MMP_RS04230 at 804.913 kb on + strand, within MMP_RS04230 at 804.913 kb on + strand, within MMP_RS04230 at 804.917 kb on - strand, within MMP_RS04230 at 804.917 kb on - strand, within MMP_RS04230 at 804.917 kb on - strand, within MMP_RS04230 at 804.918 kb on + strand, within MMP_RS04230 at 804.918 kb on + strand, within MMP_RS04230 at 804.918 kb on + strand, within MMP_RS04230 at 804.919 kb on - strand, within MMP_RS04230 at 804.919 kb on - strand, within MMP_RS04230 at 804.919 kb on - strand, within MMP_RS04230 at 804.920 kb on + strand, within MMP_RS04230 at 804.920 kb on + strand, within MMP_RS04230 at 804.921 kb on - strand, within MMP_RS04230 at 804.921 kb on - strand, within MMP_RS04230 at 804.921 kb on - strand, within MMP_RS04230 at 804.922 kb on + strand, within MMP_RS04230 at 804.922 kb on + strand, within MMP_RS04230 at 804.922 kb on + strand, within MMP_RS04230 at 804.924 kb on - strand, within MMP_RS04230 at 804.925 kb on + strand, within MMP_RS04230 at 804.925 kb on + strand, within MMP_RS04230 at 805.006 kb on - strand, within MMP_RS04230 at 805.200 kb on + strand, within MMP_RS04230 at 805.200 kb on + strand, within MMP_RS04230 at 805.234 kb on + strand, within MMP_RS04230 at 805.255 kb on - strand, within MMP_RS04230 at 805.305 kb on + strand, within MMP_RS04230 at 805.311 kb on + strand, within MMP_RS04230 at 805.316 kb on + strand, within MMP_RS04230 at 805.319 kb on - strand, within MMP_RS04230 at 805.320 kb on + strand, within MMP_RS04230 at 805.320 kb on + strand, within MMP_RS04230 at 805.320 kb on + strand, within MMP_RS04230 at 805.321 kb on - strand, within MMP_RS04230 at 805.321 kb on - strand, within MMP_RS04230 at 805.322 kb on + strand, within MMP_RS04230 at 805.322 kb on + strand, within MMP_RS04230 at 805.322 kb on + strand, within MMP_RS04230 at 805.352 kb on - strand, within MMP_RS04230 at 805.353 kb on + strand, within MMP_RS04230 at 805.353 kb on + strand, within MMP_RS04230 at 805.357 kb on - strand, within MMP_RS04230 at 805.358 kb on + strand, within MMP_RS04230 at 805.362 kb on - strand, within MMP_RS04230 at 805.362 kb on - strand, within MMP_RS04230 at 805.363 kb on + strand, within MMP_RS04230 at 805.363 kb on + strand, within MMP_RS04230 at 805.406 kb on - strand, within MMP_RS04230 at 805.452 kb on - strand, within MMP_RS04230 at 805.452 kb on - strand, within MMP_RS04230 at 805.452 kb on - strand, within MMP_RS04230 at 805.453 kb on + strand, within MMP_RS04230 at 805.454 kb on - strand, within MMP_RS04230 at 805.537 kb on + strand, within MMP_RS04230 at 805.541 kb on + strand, within MMP_RS04230 at 805.551 kb on + strand, within MMP_RS04230 at 805.551 kb on + strand, within MMP_RS04230 at 805.585 kb on - strand, within MMP_RS04230 at 805.644 kb on - strand at 805.645 kb on + strand at 805.648 kb on - strand at 805.706 kb on + strand at 805.761 kb on - strand at 805.761 kb on - strand at 805.761 kb on - strand at 805.761 kb on - strand at 805.762 kb on + strand at 805.776 kb on + strand at 805.777 kb on - strand at 805.777 kb on - strand at 805.811 kb on - strand at 805.873 kb on + strand at 805.887 kb on + strand at 805.891 kb on - strand at 805.892 kb on + strand at 805.892 kb on + strand at 805.892 kb on + strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.895 kb on - strand at 805.896 kb on + strand at 805.896 kb on + strand at 805.897 kb on - strand at 805.909 kb on + strand at 805.916 kb on + strand at 805.916 kb on + strand at 805.922 kb on - strand at 805.927 kb on - strand at 805.928 kb on + strand at 805.966 kb on - strand at 805.967 kb on + strand at 805.970 kb on - strand at 805.971 kb on + strand at 805.978 kb on + strand at 806.007 kb on - strand at 806.007 kb on - strand at 806.016 kb on + strand at 806.028 kb on + strand at 806.050 kb on - strand at 806.050 kb on - strand at 806.055 kb on + strand at 806.114 kb on - strand at 806.115 kb on + strand at 806.115 kb on + strand at 806.120 kb on - strand at 806.138 kb on - strand at 806.139 kb on + strand at 806.142 kb on - strand at 806.155 kb on - strand at 806.158 kb on - strand at 806.159 kb on + strand at 806.166 kb on + strand at 806.167 kb on - strand at 806.168 kb on + strand at 806.170 kb on - strand at 806.250 kb on + strand at 806.266 kb on + strand at 806.266 kb on + strand at 806.289 kb on + strand at 806.324 kb on + strand, within MMP_RS04235 at 806.337 kb on + strand, within MMP_RS04235 at 806.369 kb on - strand, within MMP_RS04235 at 806.501 kb on - strand, within MMP_RS04235 at 806.502 kb on + strand, within MMP_RS04235 at 806.533 kb on + strand, within MMP_RS04235 at 806.651 kb on - strand, within MMP_RS04235 at 806.652 kb on + strand, within MMP_RS04235 at 806.667 kb on + strand, within MMP_RS04235 at 806.694 kb on - strand, within MMP_RS04235 at 806.702 kb on + strand, within MMP_RS04235 at 806.711 kb on + strand, within MMP_RS04235 at 806.761 kb on - strand, within MMP_RS04235 at 806.761 kb on - strand, within MMP_RS04235 at 806.789 kb on + strand, within MMP_RS04235 at 806.791 kb on - strand, within MMP_RS04235 at 806.792 kb on + strand, within MMP_RS04235 at 806.853 kb on - strand, within MMP_RS04235
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 20 generations remove 802,723 - MMP_RS04220 0.51 -0.7 802,748 - MMP_RS04220 0.55 +0.5 802,869 - MMP_RS04220 0.75 +0.6 802,879 - MMP_RS04220 0.76 +0.9 802,880 + MMP_RS04220 0.77 -1.3 802,883 - MMP_RS04220 0.77 +0.6 802,971 - -0.3 802,971 - +2.8 802,971 - +0.6 802,971 - +0.5 802,971 - -0.9 802,972 + -0.0 802,972 + +0.8 803,016 - -0.4 803,032 - -1.9 803,033 + +0.7 803,036 - -0.7 803,036 - +1.5 803,037 + -0.9 803,046 + +0.2 803,081 + -1.9 803,126 + +0.6 803,130 - -0.0 803,151 - -0.1 803,152 + +0.2 803,152 + +2.0 803,164 + MMP_RS04225 0.11 -3.8 803,186 - MMP_RS04225 0.15 +0.9 803,196 + MMP_RS04225 0.17 +0.1 803,264 - MMP_RS04225 0.30 +0.6 803,265 + MMP_RS04225 0.30 -0.8 803,359 - MMP_RS04225 0.49 +1.0 803,405 - MMP_RS04225 0.58 -0.5 803,405 - MMP_RS04225 0.58 +0.0 803,476 + MMP_RS04225 0.72 +0.3 803,477 - MMP_RS04225 0.72 +0.2 803,622 - +0.4 803,622 - -0.1 803,623 + -0.6 803,623 + +0.5 803,624 - -0.2 803,624 - +0.3 803,624 - -0.4 803,643 - -0.2 803,643 - +0.3 803,659 + -0.6 803,663 + +0.1 803,663 + +0.1 803,663 + -0.4 803,663 + +0.0 803,664 - -0.0 803,694 - -0.8 803,694 - -0.8 803,695 + -0.3 803,703 - -0.5 803,714 - +3.5 803,714 - -0.5 803,715 + +0.3 803,715 + -0.7 803,715 + -0.4 803,826 - -0.8 803,827 + +0.3 803,852 + +0.0 803,865 + -0.3 803,921 + MMP_RS04230 0.10 +0.1 803,921 + MMP_RS04230 0.10 +0.5 803,946 - MMP_RS04230 0.11 +0.5 803,946 - MMP_RS04230 0.11 +3.6 803,946 - MMP_RS04230 0.11 -3.1 803,947 + MMP_RS04230 0.11 -0.2 804,080 + MMP_RS04230 0.18 -0.3 804,157 - MMP_RS04230 0.21 -0.3 804,173 + MMP_RS04230 0.22 +2.2 804,198 - MMP_RS04230 0.23 +0.6 804,200 - MMP_RS04230 0.23 +1.5 804,249 + MMP_RS04230 0.25 -2.0 804,296 + MMP_RS04230 0.28 +0.5 804,298 - MMP_RS04230 0.28 -0.3 804,424 - MMP_RS04230 0.34 -0.1 804,424 - MMP_RS04230 0.34 -0.5 804,515 - MMP_RS04230 0.38 -0.0 804,548 - MMP_RS04230 0.39 -1.2 804,607 + MMP_RS04230 0.42 +0.9 804,607 + MMP_RS04230 0.42 +3.1 804,625 - MMP_RS04230 0.43 +0.3 804,723 - MMP_RS04230 0.47 -1.2 804,725 - MMP_RS04230 0.47 +0.6 804,725 - MMP_RS04230 0.47 -1.2 804,725 - MMP_RS04230 0.47 +1.4 804,726 + MMP_RS04230 0.48 +0.5 804,726 + MMP_RS04230 0.48 -0.6 804,773 - MMP_RS04230 0.50 -1.3 804,774 + MMP_RS04230 0.50 -0.1 804,776 + MMP_RS04230 0.50 +0.4 804,826 - MMP_RS04230 0.52 +0.0 804,827 + MMP_RS04230 0.52 +0.0 804,827 + MMP_RS04230 0.52 -1.3 804,850 - MMP_RS04230 0.53 -2.2 804,850 - MMP_RS04230 0.53 -0.5 804,851 + MMP_RS04230 0.53 +0.1 804,851 + MMP_RS04230 0.53 +0.5 804,854 + MMP_RS04230 0.53 +1.5 804,854 + MMP_RS04230 0.53 +0.8 804,854 + MMP_RS04230 0.53 +0.2 804,855 - MMP_RS04230 0.54 -3.9 804,855 - MMP_RS04230 0.54 +0.4 804,855 - MMP_RS04230 0.54 +0.5 804,855 - MMP_RS04230 0.54 +1.2 804,855 - MMP_RS04230 0.54 -1.7 804,855 - MMP_RS04230 0.54 -2.3 804,856 + MMP_RS04230 0.54 -0.5 804,856 + MMP_RS04230 0.54 -0.0 804,856 + MMP_RS04230 0.54 -1.2 804,856 + MMP_RS04230 0.54 +0.2 804,869 - MMP_RS04230 0.54 +0.0 804,874 - MMP_RS04230 0.54 -0.9 804,874 - MMP_RS04230 0.54 +0.9 804,875 + MMP_RS04230 0.54 +0.4 804,887 + MMP_RS04230 0.55 -1.1 804,888 - MMP_RS04230 0.55 +0.8 804,910 - MMP_RS04230 0.56 -0.7 804,912 - MMP_RS04230 0.56 +1.2 804,912 - MMP_RS04230 0.56 +0.4 804,912 - MMP_RS04230 0.56 +0.1 804,912 - MMP_RS04230 0.56 -1.2 804,913 + MMP_RS04230 0.56 -0.4 804,913 + MMP_RS04230 0.56 -1.0 804,917 - MMP_RS04230 0.56 +0.4 804,917 - MMP_RS04230 0.56 +0.6 804,917 - MMP_RS04230 0.56 +0.0 804,918 + MMP_RS04230 0.56 -0.8 804,918 + MMP_RS04230 0.56 +0.2 804,918 + MMP_RS04230 0.56 -0.1 804,919 - MMP_RS04230 0.56 -0.4 804,919 - MMP_RS04230 0.56 +1.1 804,919 - MMP_RS04230 0.56 +1.1 804,920 + MMP_RS04230 0.57 +0.6 804,920 + MMP_RS04230 0.57 +0.1 804,921 - MMP_RS04230 0.57 +1.0 804,921 - MMP_RS04230 0.57 -0.2 804,921 - MMP_RS04230 0.57 +0.8 804,922 + MMP_RS04230 0.57 +0.3 804,922 + MMP_RS04230 0.57 +1.1 804,922 + MMP_RS04230 0.57 -0.4 804,924 - MMP_RS04230 0.57 -2.0 804,925 + MMP_RS04230 0.57 -0.7 804,925 + MMP_RS04230 0.57 +0.4 805,006 - MMP_RS04230 0.61 -2.0 805,200 + MMP_RS04230 0.70 -1.4 805,200 + MMP_RS04230 0.70 +0.1 805,234 + MMP_RS04230 0.71 -4.4 805,255 - MMP_RS04230 0.72 +0.5 805,305 + MMP_RS04230 0.74 +0.5 805,311 + MMP_RS04230 0.75 +2.3 805,316 + MMP_RS04230 0.75 -1.3 805,319 - MMP_RS04230 0.75 -0.4 805,320 + MMP_RS04230 0.75 +0.9 805,320 + MMP_RS04230 0.75 +0.1 805,320 + MMP_RS04230 0.75 +0.6 805,321 - MMP_RS04230 0.75 +1.0 805,321 - MMP_RS04230 0.75 +2.6 805,322 + MMP_RS04230 0.75 -0.5 805,322 + MMP_RS04230 0.75 -0.5 805,322 + MMP_RS04230 0.75 +0.6 805,352 - MMP_RS04230 0.77 +0.1 805,353 + MMP_RS04230 0.77 +1.5 805,353 + MMP_RS04230 0.77 -0.3 805,357 - MMP_RS04230 0.77 +0.4 805,358 + MMP_RS04230 0.77 +1.5 805,362 - MMP_RS04230 0.77 +0.6 805,362 - MMP_RS04230 0.77 -1.0 805,363 + MMP_RS04230 0.77 -0.9 805,363 + MMP_RS04230 0.77 +1.6 805,406 - MMP_RS04230 0.79 -1.2 805,452 - MMP_RS04230 0.81 -1.4 805,452 - MMP_RS04230 0.81 +0.4 805,452 - MMP_RS04230 0.81 +0.9 805,453 + MMP_RS04230 0.81 -0.9 805,454 - MMP_RS04230 0.81 +0.4 805,537 + MMP_RS04230 0.85 -0.1 805,541 + MMP_RS04230 0.85 -0.0 805,551 + MMP_RS04230 0.86 +0.6 805,551 + MMP_RS04230 0.86 -2.4 805,585 - MMP_RS04230 0.87 -0.3 805,644 - -0.0 805,645 + -0.4 805,648 - +0.0 805,706 + -0.1 805,761 - -0.2 805,761 - -2.6 805,761 - +1.1 805,761 - -1.2 805,762 + -0.0 805,776 + -0.6 805,777 - -0.6 805,777 - +0.3 805,811 - -0.8 805,873 + -0.8 805,887 + +0.4 805,891 - +0.7 805,892 + +0.1 805,892 + +0.8 805,892 + -0.1 805,895 - -1.5 805,895 - -2.0 805,895 - +0.6 805,895 - +0.0 805,895 - +0.8 805,895 - -0.6 805,895 - +0.7 805,895 - +0.5 805,896 + +0.5 805,896 + +0.5 805,897 - +1.1 805,909 + +0.8 805,916 + +0.4 805,916 + -0.6 805,922 - -1.0 805,927 - -0.9 805,928 + +2.7 805,966 - +0.3 805,967 + -0.9 805,970 - +0.5 805,971 + +0.3 805,978 + -0.3 806,007 - -2.1 806,007 - +0.5 806,016 + +1.3 806,028 + +0.7 806,050 - -0.1 806,050 - +1.7 806,055 + -0.7 806,114 - +0.5 806,115 + -0.5 806,115 + +0.3 806,120 - +1.1 806,138 - +1.1 806,139 + +0.6 806,142 - +0.5 806,155 - +0.4 806,158 - +0.9 806,159 + -0.0 806,166 + -0.2 806,167 - -0.9 806,168 + +0.7 806,170 - +1.0 806,250 + +0.5 806,266 + +0.5 806,266 + -0.8 806,289 + -1.3 806,324 + MMP_RS04235 0.12 +0.7 806,337 + MMP_RS04235 0.13 -1.5 806,369 - MMP_RS04235 0.16 +0.5 806,501 - MMP_RS04235 0.28 -0.3 806,502 + MMP_RS04235 0.28 -0.2 806,533 + MMP_RS04235 0.31 +0.9 806,651 - MMP_RS04235 0.42 +0.4 806,652 + MMP_RS04235 0.42 +1.1 806,667 + MMP_RS04235 0.43 -0.4 806,694 - MMP_RS04235 0.45 +2.0 806,702 + MMP_RS04235 0.46 -0.1 806,711 + MMP_RS04235 0.47 -0.5 806,761 - MMP_RS04235 0.51 -0.7 806,761 - MMP_RS04235 0.51 -0.2 806,789 + MMP_RS04235 0.54 +0.3 806,791 - MMP_RS04235 0.54 +0.3 806,792 + MMP_RS04235 0.54 -1.0 806,853 - MMP_RS04235 0.60 -0.7
Or see this region's nucleotide sequence