Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04205 and MMP_RS04210 are separated by 145 nucleotides MMP_RS04210 and MMP_RS04215 are separated by 11 nucleotides MMP_RS04215 and MMP_RS04220 are separated by 48 nucleotides
MMP_RS04205: MMP_RS04205 - carboxymuconolactone decarboxylase family protein, at 799,477 to 800,229
_RS04205
MMP_RS04210: MMP_RS04210 - aldo/keto reductase, at 800,375 to 801,568
_RS04210
MMP_RS04215: MMP_RS04215 - nickel pincer cofactor biosynthesis protein LarB, at 801,580 to 802,365
_RS04215
MMP_RS04220: MMP_RS04220 - queuosine precursor transporter, at 802,414 to 803,022
_RS04220
Position (kb) 800
801
802 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 799.470 kb on - strand at 799.630 kb on + strand, within MMP_RS04205 at 799.684 kb on + strand, within MMP_RS04205 at 799.762 kb on - strand, within MMP_RS04205 at 799.763 kb on + strand, within MMP_RS04205 at 799.763 kb on + strand, within MMP_RS04205 at 799.814 kb on - strand, within MMP_RS04205 at 799.854 kb on - strand, within MMP_RS04205 at 799.972 kb on - strand, within MMP_RS04205 at 800.167 kb on - strand at 800.167 kb on - strand at 800.171 kb on - strand at 800.171 kb on - strand at 800.217 kb on + strand at 800.247 kb on + strand at 800.247 kb on + strand at 800.261 kb on + strand at 800.266 kb on - strand at 800.267 kb on + strand at 800.270 kb on + strand at 800.294 kb on - strand at 800.295 kb on + strand at 800.295 kb on + strand at 800.295 kb on + strand at 800.295 kb on + strand at 800.297 kb on + strand at 800.297 kb on + strand at 800.300 kb on - strand at 800.308 kb on - strand at 800.309 kb on + strand at 800.309 kb on + strand at 800.309 kb on + strand at 800.309 kb on + strand at 800.309 kb on + strand at 800.309 kb on + strand at 800.309 kb on + strand at 800.312 kb on - strand at 800.313 kb on + strand at 800.315 kb on - strand at 800.315 kb on - strand at 800.315 kb on - strand at 800.315 kb on - strand at 800.317 kb on - strand at 800.317 kb on - strand at 800.318 kb on + strand at 800.318 kb on + strand at 800.341 kb on + strand at 800.351 kb on - strand at 800.352 kb on + strand at 800.354 kb on - strand at 800.355 kb on + strand at 800.357 kb on + strand at 800.381 kb on - strand at 800.381 kb on - strand at 800.382 kb on + strand at 800.434 kb on - strand at 800.671 kb on + strand, within MMP_RS04210 at 800.719 kb on + strand, within MMP_RS04210 at 800.871 kb on + strand, within MMP_RS04210 at 800.872 kb on - strand, within MMP_RS04210 at 800.873 kb on + strand, within MMP_RS04210 at 800.873 kb on + strand, within MMP_RS04210 at 800.892 kb on - strand, within MMP_RS04210 at 800.892 kb on - strand, within MMP_RS04210 at 800.892 kb on - strand, within MMP_RS04210 at 800.893 kb on + strand, within MMP_RS04210 at 800.893 kb on + strand, within MMP_RS04210 at 800.893 kb on + strand, within MMP_RS04210 at 800.899 kb on - strand, within MMP_RS04210 at 800.899 kb on - strand, within MMP_RS04210 at 800.899 kb on - strand, within MMP_RS04210 at 800.900 kb on + strand, within MMP_RS04210 at 800.900 kb on + strand, within MMP_RS04210 at 800.903 kb on - strand, within MMP_RS04210 at 800.903 kb on - strand, within MMP_RS04210 at 800.904 kb on + strand, within MMP_RS04210 at 800.904 kb on + strand, within MMP_RS04210 at 800.941 kb on - strand, within MMP_RS04210 at 801.003 kb on - strand, within MMP_RS04210 at 801.010 kb on - strand, within MMP_RS04210 at 801.037 kb on + strand, within MMP_RS04210 at 801.069 kb on - strand, within MMP_RS04210 at 801.232 kb on + strand, within MMP_RS04210 at 801.257 kb on + strand, within MMP_RS04210 at 801.257 kb on + strand, within MMP_RS04210 at 801.262 kb on - strand, within MMP_RS04210 at 801.326 kb on - strand, within MMP_RS04210 at 801.326 kb on - strand, within MMP_RS04210 at 801.326 kb on - strand, within MMP_RS04210 at 801.391 kb on - strand, within MMP_RS04210 at 801.392 kb on + strand, within MMP_RS04210 at 801.394 kb on + strand, within MMP_RS04210 at 801.442 kb on - strand, within MMP_RS04210 at 801.467 kb on - strand at 801.467 kb on - strand at 801.467 kb on - strand at 801.468 kb on + strand at 801.468 kb on + strand at 801.597 kb on + strand at 801.608 kb on - strand at 801.648 kb on - strand at 801.665 kb on + strand, within MMP_RS04215 at 801.687 kb on - strand, within MMP_RS04215 at 801.696 kb on - strand, within MMP_RS04215 at 801.696 kb on - strand, within MMP_RS04215 at 801.697 kb on + strand, within MMP_RS04215 at 801.697 kb on + strand, within MMP_RS04215 at 801.895 kb on + strand, within MMP_RS04215 at 802.035 kb on + strand, within MMP_RS04215 at 802.035 kb on + strand, within MMP_RS04215 at 802.037 kb on + strand, within MMP_RS04215 at 802.037 kb on + strand, within MMP_RS04215 at 802.265 kb on - strand, within MMP_RS04215 at 802.407 kb on + strand at 802.443 kb on + strand at 802.445 kb on + strand at 802.469 kb on + strand at 802.474 kb on + strand at 802.529 kb on + strand, within MMP_RS04220
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 20 generations remove 799,470 - +0.5 799,630 + MMP_RS04205 0.20 +0.0 799,684 + MMP_RS04205 0.27 +0.3 799,762 - MMP_RS04205 0.38 -0.5 799,763 + MMP_RS04205 0.38 +1.0 799,763 + MMP_RS04205 0.38 +0.8 799,814 - MMP_RS04205 0.45 +0.5 799,854 - MMP_RS04205 0.50 +1.9 799,972 - MMP_RS04205 0.66 -0.8 800,167 - +0.1 800,167 - +0.2 800,171 - +0.3 800,171 - +1.8 800,217 + +0.6 800,247 + -0.4 800,247 + -1.6 800,261 + -0.5 800,266 - -0.8 800,267 + -0.6 800,270 + -0.6 800,294 - +0.4 800,295 + -0.7 800,295 + -0.0 800,295 + -1.4 800,295 + -1.1 800,297 + -1.3 800,297 + +0.9 800,300 - -2.1 800,308 - -0.6 800,309 + +0.9 800,309 + -0.6 800,309 + -0.0 800,309 + -0.6 800,309 + -2.1 800,309 + -0.6 800,309 + +0.2 800,312 - -0.5 800,313 + -2.3 800,315 - -1.2 800,315 - -0.4 800,315 - -0.3 800,315 - +0.7 800,317 - -0.0 800,317 - -0.2 800,318 + -1.1 800,318 + -0.1 800,341 + -0.2 800,351 - -1.4 800,352 + +0.3 800,354 - -0.6 800,355 + +2.4 800,357 + +0.4 800,381 - -0.3 800,381 - +0.4 800,382 + -0.9 800,434 - -1.0 800,671 + MMP_RS04210 0.25 -0.7 800,719 + MMP_RS04210 0.29 -2.9 800,871 + MMP_RS04210 0.42 -1.6 800,872 - MMP_RS04210 0.42 -1.0 800,873 + MMP_RS04210 0.42 -0.8 800,873 + MMP_RS04210 0.42 -0.1 800,892 - MMP_RS04210 0.43 -1.1 800,892 - MMP_RS04210 0.43 +1.6 800,892 - MMP_RS04210 0.43 -0.2 800,893 + MMP_RS04210 0.43 -1.5 800,893 + MMP_RS04210 0.43 -1.0 800,893 + MMP_RS04210 0.43 +0.3 800,899 - MMP_RS04210 0.44 -1.4 800,899 - MMP_RS04210 0.44 -0.8 800,899 - MMP_RS04210 0.44 -0.8 800,900 + MMP_RS04210 0.44 +0.1 800,900 + MMP_RS04210 0.44 -0.8 800,903 - MMP_RS04210 0.44 +0.7 800,903 - MMP_RS04210 0.44 -1.5 800,904 + MMP_RS04210 0.44 -1.0 800,904 + MMP_RS04210 0.44 -0.1 800,941 - MMP_RS04210 0.47 -3.8 801,003 - MMP_RS04210 0.53 -0.6 801,010 - MMP_RS04210 0.53 -1.8 801,037 + MMP_RS04210 0.55 -1.3 801,069 - MMP_RS04210 0.58 -0.4 801,232 + MMP_RS04210 0.72 -1.4 801,257 + MMP_RS04210 0.74 -0.5 801,257 + MMP_RS04210 0.74 -1.8 801,262 - MMP_RS04210 0.74 -6.1 801,326 - MMP_RS04210 0.80 -4.7 801,326 - MMP_RS04210 0.80 -4.6 801,326 - MMP_RS04210 0.80 -3.2 801,391 - MMP_RS04210 0.85 -3.7 801,392 + MMP_RS04210 0.85 -1.4 801,394 + MMP_RS04210 0.85 -0.2 801,442 - MMP_RS04210 0.89 -3.9 801,467 - -2.3 801,467 - -4.2 801,467 - -1.4 801,468 + +2.9 801,468 + -1.5 801,597 + -4.6 801,608 - -5.9 801,648 - -5.5 801,665 + MMP_RS04215 0.11 -5.7 801,687 - MMP_RS04215 0.14 -4.1 801,696 - MMP_RS04215 0.15 -4.7 801,696 - MMP_RS04215 0.15 -1.6 801,697 + MMP_RS04215 0.15 -4.7 801,697 + MMP_RS04215 0.15 -5.6 801,895 + MMP_RS04215 0.40 -5.6 802,035 + MMP_RS04215 0.58 -3.8 802,035 + MMP_RS04215 0.58 -4.4 802,037 + MMP_RS04215 0.58 -2.8 802,037 + MMP_RS04215 0.58 -5.7 802,265 - MMP_RS04215 0.87 -1.9 802,407 + +0.4 802,443 + -0.6 802,445 + +0.3 802,469 + +1.5 802,474 + -1.0 802,529 + MMP_RS04220 0.19 +0.5
Or see this region's nucleotide sequence