Strain Fitness in Rahnella sp. WP5 around EX31_RS18495

Experiment: L-Serine (N); ShewMM media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS18485 and EX31_RS18490 are separated by 69 nucleotidesEX31_RS18490 and EX31_RS18495 are separated by 1 nucleotidesEX31_RS18495 and EX31_RS18500 are separated by 11 nucleotides EX31_RS18485: EX31_RS18485 - Tat proofreading chaperone DmsD, at 266,704 to 267,312 _RS18485 EX31_RS18490: EX31_RS18490 - dimethyl sulfoxide reductase anchor subunit, at 267,382 to 268,239 _RS18490 EX31_RS18495: EX31_RS18495 - dimethylsulfoxide reductase subunit B, at 268,241 to 268,858 _RS18495 EX31_RS18500: EX31_RS18500 - dimethylsulfoxide reductase subunit A, at 268,870 to 271,317 _RS18500 Position (kb) 268 269Strain fitness (log2 ratio) -2 -1 0 1 2 3at 267.294 kb on + strandat 267.313 kb on - strandat 267.313 kb on - strandat 267.343 kb on - strandat 267.411 kb on + strandat 267.411 kb on + strandat 267.412 kb on - strandat 267.423 kb on - strandat 267.502 kb on - strand, within EX31_RS18490at 267.509 kb on - strand, within EX31_RS18490at 267.509 kb on - strand, within EX31_RS18490at 267.509 kb on - strand, within EX31_RS18490at 267.509 kb on - strand, within EX31_RS18490at 267.509 kb on - strand, within EX31_RS18490at 267.509 kb on - strand, within EX31_RS18490at 267.511 kb on - strand, within EX31_RS18490at 267.511 kb on - strand, within EX31_RS18490at 267.511 kb on - strand, within EX31_RS18490at 267.511 kb on - strand, within EX31_RS18490at 267.511 kb on - strand, within EX31_RS18490at 267.511 kb on - strand, within EX31_RS18490at 267.511 kb on - strand, within EX31_RS18490at 267.579 kb on - strand, within EX31_RS18490at 267.661 kb on - strand, within EX31_RS18490at 267.661 kb on - strand, within EX31_RS18490at 267.661 kb on - strand, within EX31_RS18490at 267.704 kb on - strand, within EX31_RS18490at 267.717 kb on + strand, within EX31_RS18490at 267.718 kb on - strand, within EX31_RS18490at 267.718 kb on - strand, within EX31_RS18490at 267.718 kb on - strand, within EX31_RS18490at 267.720 kb on + strand, within EX31_RS18490at 267.720 kb on + strand, within EX31_RS18490at 267.720 kb on + strand, within EX31_RS18490at 267.721 kb on - strand, within EX31_RS18490at 267.721 kb on - strand, within EX31_RS18490at 267.721 kb on - strand, within EX31_RS18490at 267.721 kb on - strand, within EX31_RS18490at 267.721 kb on - strand, within EX31_RS18490at 267.723 kb on + strand, within EX31_RS18490at 267.723 kb on + strand, within EX31_RS18490at 267.724 kb on - strand, within EX31_RS18490at 267.724 kb on - strand, within EX31_RS18490at 267.744 kb on - strand, within EX31_RS18490at 267.795 kb on + strand, within EX31_RS18490at 267.798 kb on - strand, within EX31_RS18490at 267.798 kb on - strand, within EX31_RS18490at 267.822 kb on + strand, within EX31_RS18490at 267.823 kb on - strand, within EX31_RS18490at 267.833 kb on + strand, within EX31_RS18490at 267.833 kb on + strand, within EX31_RS18490at 267.834 kb on - strand, within EX31_RS18490at 267.834 kb on - strand, within EX31_RS18490at 267.834 kb on - strand, within EX31_RS18490at 267.834 kb on - strand, within EX31_RS18490at 267.834 kb on - strand, within EX31_RS18490at 267.941 kb on + strand, within EX31_RS18490at 267.941 kb on + strand, within EX31_RS18490at 267.941 kb on + strand, within EX31_RS18490at 268.122 kb on - strand, within EX31_RS18490at 268.168 kb on - strandat 268.168 kb on - strandat 268.267 kb on - strandat 268.267 kb on - strandat 268.277 kb on - strandat 268.310 kb on - strand, within EX31_RS18495at 268.310 kb on - strand, within EX31_RS18495at 268.319 kb on - strand, within EX31_RS18495at 268.319 kb on - strand, within EX31_RS18495at 268.375 kb on - strand, within EX31_RS18495at 268.375 kb on - strand, within EX31_RS18495at 268.470 kb on + strand, within EX31_RS18495at 268.471 kb on - strand, within EX31_RS18495at 268.512 kb on + strand, within EX31_RS18495at 268.513 kb on - strand, within EX31_RS18495at 268.513 kb on - strand, within EX31_RS18495at 268.561 kb on + strand, within EX31_RS18495at 268.568 kb on - strand, within EX31_RS18495at 268.568 kb on - strand, within EX31_RS18495at 268.568 kb on - strand, within EX31_RS18495at 268.604 kb on - strand, within EX31_RS18495at 268.674 kb on + strand, within EX31_RS18495at 268.674 kb on + strand, within EX31_RS18495at 268.678 kb on - strand, within EX31_RS18495at 268.734 kb on + strand, within EX31_RS18495at 268.740 kb on + strand, within EX31_RS18495at 268.740 kb on + strand, within EX31_RS18495at 268.740 kb on + strand, within EX31_RS18495at 268.740 kb on + strand, within EX31_RS18495at 268.740 kb on + strand, within EX31_RS18495at 268.741 kb on - strand, within EX31_RS18495at 268.741 kb on - strand, within EX31_RS18495at 268.741 kb on - strand, within EX31_RS18495at 268.741 kb on - strand, within EX31_RS18495at 268.744 kb on + strand, within EX31_RS18495at 268.834 kb on + strandat 268.836 kb on + strandat 268.837 kb on - strandat 268.900 kb on - strandat 268.900 kb on - strandat 268.912 kb on - strandat 268.948 kb on + strandat 268.949 kb on - strandat 268.949 kb on - strandat 268.949 kb on - strandat 268.949 kb on - strandat 268.949 kb on - strandat 268.949 kb on - strandat 268.953 kb on + strandat 268.954 kb on - strandat 269.056 kb on + strandat 269.057 kb on - strandat 269.058 kb on + strandat 269.058 kb on + strandat 269.058 kb on + strandat 269.058 kb on + strandat 269.059 kb on - strandat 269.059 kb on - strandat 269.109 kb on + strandat 269.171 kb on - strand, within EX31_RS18500at 269.186 kb on + strand, within EX31_RS18500at 269.208 kb on + strand, within EX31_RS18500at 269.209 kb on - strand, within EX31_RS18500at 269.210 kb on + strand, within EX31_RS18500at 269.210 kb on + strand, within EX31_RS18500at 269.238 kb on - strand, within EX31_RS18500at 269.238 kb on - strand, within EX31_RS18500at 269.238 kb on - strand, within EX31_RS18500at 269.262 kb on - strand, within EX31_RS18500at 269.262 kb on - strand, within EX31_RS18500at 269.282 kb on + strand, within EX31_RS18500at 269.282 kb on + strand, within EX31_RS18500at 269.283 kb on - strand, within EX31_RS18500at 269.357 kb on + strand, within EX31_RS18500at 269.423 kb on + strand, within EX31_RS18500at 269.424 kb on - strand, within EX31_RS18500at 269.526 kb on - strand, within EX31_RS18500at 269.553 kb on + strand, within EX31_RS18500at 269.615 kb on + strand, within EX31_RS18500at 269.615 kb on + strand, within EX31_RS18500at 269.616 kb on - strand, within EX31_RS18500at 269.616 kb on - strand, within EX31_RS18500at 269.669 kb on + strand, within EX31_RS18500at 269.669 kb on + strand, within EX31_RS18500at 269.742 kb on + strand, within EX31_RS18500at 269.743 kb on - strand, within EX31_RS18500at 269.743 kb on - strand, within EX31_RS18500at 269.743 kb on - strand, within EX31_RS18500at 269.750 kb on + strand, within EX31_RS18500

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking
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267,294 + +1.1
267,313 - +0.8
267,313 - +1.9
267,343 - +1.2
267,411 + +2.9
267,411 + -0.2
267,412 - +0.8
267,423 - +1.1
267,502 - EX31_RS18490 0.14 -1.8
267,509 - EX31_RS18490 0.15 +0.8
267,509 - EX31_RS18490 0.15 +0.9
267,509 - EX31_RS18490 0.15 -0.5
267,509 - EX31_RS18490 0.15 +0.7
267,509 - EX31_RS18490 0.15 -0.0
267,509 - EX31_RS18490 0.15 +1.0
267,511 - EX31_RS18490 0.15 -0.4
267,511 - EX31_RS18490 0.15 -0.6
267,511 - EX31_RS18490 0.15 +0.5
267,511 - EX31_RS18490 0.15 -1.1
267,511 - EX31_RS18490 0.15 +0.9
267,511 - EX31_RS18490 0.15 +0.5
267,511 - EX31_RS18490 0.15 +0.5
267,579 - EX31_RS18490 0.23 -0.3
267,661 - EX31_RS18490 0.33 +1.6
267,661 - EX31_RS18490 0.33 -2.3
267,661 - EX31_RS18490 0.33 -1.0
267,704 - EX31_RS18490 0.38 -1.0
267,717 + EX31_RS18490 0.39 -0.6
267,718 - EX31_RS18490 0.39 +1.7
267,718 - EX31_RS18490 0.39 -0.1
267,718 - EX31_RS18490 0.39 -1.1
267,720 + EX31_RS18490 0.39 -2.4
267,720 + EX31_RS18490 0.39 -0.3
267,720 + EX31_RS18490 0.39 +0.4
267,721 - EX31_RS18490 0.40 -0.9
267,721 - EX31_RS18490 0.40 +1.9
267,721 - EX31_RS18490 0.40 +0.9
267,721 - EX31_RS18490 0.40 +1.7
267,721 - EX31_RS18490 0.40 +1.3
267,723 + EX31_RS18490 0.40 -0.5
267,723 + EX31_RS18490 0.40 +1.2
267,724 - EX31_RS18490 0.40 +2.1
267,724 - EX31_RS18490 0.40 +0.5
267,744 - EX31_RS18490 0.42 +0.3
267,795 + EX31_RS18490 0.48 +1.6
267,798 - EX31_RS18490 0.48 -0.2
267,798 - EX31_RS18490 0.48 -0.1
267,822 + EX31_RS18490 0.51 +0.7
267,823 - EX31_RS18490 0.51 -0.6
267,833 + EX31_RS18490 0.53 -0.5
267,833 + EX31_RS18490 0.53 +2.1
267,834 - EX31_RS18490 0.53 -0.3
267,834 - EX31_RS18490 0.53 +0.6
267,834 - EX31_RS18490 0.53 -0.0
267,834 - EX31_RS18490 0.53 -1.2
267,834 - EX31_RS18490 0.53 -1.5
267,941 + EX31_RS18490 0.65 +0.5
267,941 + EX31_RS18490 0.65 -0.0
267,941 + EX31_RS18490 0.65 -0.5
268,122 - EX31_RS18490 0.86 +0.2
268,168 - -1.1
268,168 - +0.4
268,267 - -0.7
268,267 - -0.1
268,277 - +0.9
268,310 - EX31_RS18495 0.11 +1.1
268,310 - EX31_RS18495 0.11 +0.6
268,319 - EX31_RS18495 0.13 +1.2
268,319 - EX31_RS18495 0.13 -0.6
268,375 - EX31_RS18495 0.22 -0.0
268,375 - EX31_RS18495 0.22 +1.0
268,470 + EX31_RS18495 0.37 -0.6
268,471 - EX31_RS18495 0.37 +0.4
268,512 + EX31_RS18495 0.44 +1.5
268,513 - EX31_RS18495 0.44 -0.9
268,513 - EX31_RS18495 0.44 +1.4
268,561 + EX31_RS18495 0.52 +0.6
268,568 - EX31_RS18495 0.53 -0.8
268,568 - EX31_RS18495 0.53 -0.2
268,568 - EX31_RS18495 0.53 +0.8
268,604 - EX31_RS18495 0.59 -0.2
268,674 + EX31_RS18495 0.70 -0.9
268,674 + EX31_RS18495 0.70 +1.1
268,678 - EX31_RS18495 0.71 -0.1
268,734 + EX31_RS18495 0.80 +1.9
268,740 + EX31_RS18495 0.81 +0.5
268,740 + EX31_RS18495 0.81 +0.7
268,740 + EX31_RS18495 0.81 -1.1
268,740 + EX31_RS18495 0.81 -1.1
268,740 + EX31_RS18495 0.81 -1.1
268,741 - EX31_RS18495 0.81 -1.1
268,741 - EX31_RS18495 0.81 +0.6
268,741 - EX31_RS18495 0.81 +0.7
268,741 - EX31_RS18495 0.81 -0.7
268,744 + EX31_RS18495 0.81 +2.9
268,834 + +0.7
268,836 + +0.1
268,837 - +1.0
268,900 - +0.7
268,900 - -1.6
268,912 - -0.4
268,948 + +1.4
268,949 - -0.6
268,949 - -0.5
268,949 - -0.9
268,949 - +2.0
268,949 - +0.6
268,949 - +0.2
268,953 + +0.3
268,954 - +2.2
269,056 + +0.5
269,057 - -0.7
269,058 + +0.2
269,058 + +0.3
269,058 + +0.3
269,058 + +1.6
269,059 - -1.8
269,059 - -0.4
269,109 + -0.5
269,171 - EX31_RS18500 0.12 +0.0
269,186 + EX31_RS18500 0.13 +1.2
269,208 + EX31_RS18500 0.14 -0.0
269,209 - EX31_RS18500 0.14 +1.3
269,210 + EX31_RS18500 0.14 +0.5
269,210 + EX31_RS18500 0.14 +0.9
269,238 - EX31_RS18500 0.15 +0.9
269,238 - EX31_RS18500 0.15 -1.3
269,238 - EX31_RS18500 0.15 -0.5
269,262 - EX31_RS18500 0.16 +0.2
269,262 - EX31_RS18500 0.16 +0.7
269,282 + EX31_RS18500 0.17 -2.0
269,282 + EX31_RS18500 0.17 +0.8
269,283 - EX31_RS18500 0.17 -0.9
269,357 + EX31_RS18500 0.20 +1.9
269,423 + EX31_RS18500 0.23 +0.8
269,424 - EX31_RS18500 0.23 +2.0
269,526 - EX31_RS18500 0.27 +1.3
269,553 + EX31_RS18500 0.28 +0.9
269,615 + EX31_RS18500 0.30 -1.4
269,615 + EX31_RS18500 0.30 -0.4
269,616 - EX31_RS18500 0.30 +0.2
269,616 - EX31_RS18500 0.30 +0.6
269,669 + EX31_RS18500 0.33 +0.9
269,669 + EX31_RS18500 0.33 +1.9
269,742 + EX31_RS18500 0.36 -0.1
269,743 - EX31_RS18500 0.36 +0.1
269,743 - EX31_RS18500 0.36 +2.6
269,743 - EX31_RS18500 0.36 +1.3
269,750 + EX31_RS18500 0.36 -0.4

Or see this region's nucleotide sequence