Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17280 and EX31_RS17285 are separated by 136 nucleotides EX31_RS17285 and EX31_RS17290 overlap by 4 nucleotides EX31_RS17290 and EX31_RS17295 are separated by 171 nucleotides EX31_RS17295 and EX31_RS17300 are separated by 309 nucleotides
EX31_RS17280: EX31_RS17280 - membrane protein, at 6,235 to 6,630
_RS17280
EX31_RS17285: EX31_RS17285 - carboxy-S-adenosyl-L-methionine synthase CmoA, at 6,767 to 7,510
_RS17285
EX31_RS17290: EX31_RS17290 - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 7,507 to 8,478
_RS17290
EX31_RS17295: EX31_RS17295 - hypothetical protein, at 8,650 to 8,853
_RS17295
EX31_RS17300: EX31_RS17300 - hypothetical protein, at 9,163 to 9,666
_RS17300
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6.556 kb on + strand, within EX31_RS17280 at 6.556 kb on + strand, within EX31_RS17280 at 6.556 kb on + strand, within EX31_RS17280 at 6.556 kb on + strand, within EX31_RS17280 at 6.556 kb on + strand, within EX31_RS17280 at 6.556 kb on + strand, within EX31_RS17280 at 6.556 kb on + strand, within EX31_RS17280 at 6.556 kb on + strand, within EX31_RS17280 at 6.556 kb on + strand, within EX31_RS17280 at 6.556 kb on + strand, within EX31_RS17280 at 6.557 kb on - strand, within EX31_RS17280 at 6.557 kb on - strand, within EX31_RS17280 at 6.557 kb on - strand, within EX31_RS17280 at 6.557 kb on - strand, within EX31_RS17280 at 6.557 kb on - strand, within EX31_RS17280 at 6.558 kb on + strand, within EX31_RS17280 at 6.558 kb on + strand, within EX31_RS17280 at 6.559 kb on - strand, within EX31_RS17280 at 6.559 kb on - strand, within EX31_RS17280 at 6.559 kb on - strand, within EX31_RS17280 at 6.559 kb on - strand, within EX31_RS17280 at 6.588 kb on + strand, within EX31_RS17280 at 6.588 kb on + strand, within EX31_RS17280 at 6.588 kb on + strand, within EX31_RS17280 at 6.588 kb on + strand, within EX31_RS17280 at 6.589 kb on - strand, within EX31_RS17280 at 6.589 kb on - strand, within EX31_RS17280 at 6.591 kb on + strand at 6.591 kb on + strand at 6.591 kb on + strand at 6.591 kb on + strand at 6.591 kb on + strand at 6.592 kb on - strand at 6.592 kb on - strand at 6.595 kb on + strand at 6.595 kb on + strand at 6.596 kb on - strand at 6.596 kb on - strand at 6.596 kb on - strand at 6.596 kb on - strand at 6.691 kb on + strand at 6.707 kb on + strand at 6.708 kb on - strand at 6.736 kb on - strand at 6.799 kb on + strand at 6.799 kb on + strand at 6.799 kb on + strand at 6.800 kb on - strand at 6.800 kb on - strand at 6.800 kb on - strand at 6.800 kb on - strand at 6.801 kb on + strand at 6.801 kb on + strand at 6.801 kb on + strand at 6.801 kb on + strand at 6.801 kb on + strand at 6.802 kb on - strand at 6.802 kb on - strand at 6.802 kb on - strand at 6.802 kb on - strand at 6.805 kb on + strand at 6.805 kb on + strand at 6.805 kb on + strand at 6.805 kb on + strand at 6.805 kb on + strand at 6.806 kb on - strand at 6.806 kb on - strand at 6.806 kb on - strand at 6.806 kb on - strand at 6.892 kb on + strand, within EX31_RS17285 at 6.947 kb on + strand, within EX31_RS17285 at 6.947 kb on + strand, within EX31_RS17285 at 6.947 kb on + strand, within EX31_RS17285 at 6.947 kb on + strand, within EX31_RS17285 at 6.948 kb on - strand, within EX31_RS17285 at 6.994 kb on + strand, within EX31_RS17285 at 7.000 kb on + strand, within EX31_RS17285 at 7.152 kb on + strand, within EX31_RS17285 at 7.152 kb on + strand, within EX31_RS17285 at 7.217 kb on + strand, within EX31_RS17285 at 7.217 kb on + strand, within EX31_RS17285 at 7.218 kb on - strand, within EX31_RS17285 at 7.303 kb on + strand, within EX31_RS17285 at 7.381 kb on + strand, within EX31_RS17285 at 7.382 kb on - strand, within EX31_RS17285 at 7.553 kb on + strand at 7.569 kb on + strand at 7.570 kb on - strand at 7.758 kb on + strand, within EX31_RS17290 at 7.761 kb on + strand, within EX31_RS17290 at 7.761 kb on + strand, within EX31_RS17290 at 7.761 kb on + strand, within EX31_RS17290 at 7.762 kb on - strand, within EX31_RS17290 at 7.886 kb on + strand, within EX31_RS17290 at 8.001 kb on + strand, within EX31_RS17290 at 8.123 kb on + strand, within EX31_RS17290 at 8.124 kb on - strand, within EX31_RS17290 at 8.124 kb on - strand, within EX31_RS17290 at 8.199 kb on + strand, within EX31_RS17290 at 8.250 kb on + strand, within EX31_RS17290 at 8.250 kb on + strand, within EX31_RS17290 at 8.250 kb on + strand, within EX31_RS17290 at 8.251 kb on - strand, within EX31_RS17290 at 8.254 kb on + strand, within EX31_RS17290 at 8.254 kb on + strand, within EX31_RS17290 at 8.319 kb on + strand, within EX31_RS17290 at 8.424 kb on + strand at 8.424 kb on + strand at 8.477 kb on - strand at 8.537 kb on - strand at 8.565 kb on - strand at 8.577 kb on - strand at 8.649 kb on + strand at 8.677 kb on - strand, within EX31_RS17295 at 8.746 kb on + strand, within EX31_RS17295 at 8.747 kb on - strand, within EX31_RS17295 at 8.747 kb on - strand, within EX31_RS17295 at 8.750 kb on - strand, within EX31_RS17295 at 8.750 kb on - strand, within EX31_RS17295 at 8.811 kb on + strand, within EX31_RS17295 at 8.812 kb on - strand, within EX31_RS17295 at 8.903 kb on + strand at 8.903 kb on + strand at 8.904 kb on - strand at 8.905 kb on + strand at 8.906 kb on - strand at 8.937 kb on + strand at 8.963 kb on - strand at 9.241 kb on - strand, within EX31_RS17300 at 9.247 kb on - strand, within EX31_RS17300 at 9.247 kb on - strand, within EX31_RS17300 at 9.315 kb on - strand, within EX31_RS17300 at 9.323 kb on + strand, within EX31_RS17300 at 9.323 kb on + strand, within EX31_RS17300 at 9.323 kb on + strand, within EX31_RS17300 at 9.324 kb on - strand, within EX31_RS17300 at 9.324 kb on - strand, within EX31_RS17300 at 9.324 kb on - strand, within EX31_RS17300 at 9.324 kb on - strand, within EX31_RS17300 at 9.441 kb on + strand, within EX31_RS17300 at 9.464 kb on + strand, within EX31_RS17300 at 9.465 kb on - strand, within EX31_RS17300 at 9.465 kb on - strand, within EX31_RS17300 at 9.465 kb on - strand, within EX31_RS17300 at 9.465 kb on - strand, within EX31_RS17300
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 6,556 + EX31_RS17280 0.81 +1.2 6,556 + EX31_RS17280 0.81 -0.3 6,556 + EX31_RS17280 0.81 -0.5 6,556 + EX31_RS17280 0.81 -0.2 6,556 + EX31_RS17280 0.81 -0.1 6,556 + EX31_RS17280 0.81 -0.2 6,556 + EX31_RS17280 0.81 +0.7 6,556 + EX31_RS17280 0.81 +1.0 6,556 + EX31_RS17280 0.81 -0.9 6,556 + EX31_RS17280 0.81 +0.3 6,557 - EX31_RS17280 0.81 -0.7 6,557 - EX31_RS17280 0.81 -0.4 6,557 - EX31_RS17280 0.81 -0.3 6,557 - EX31_RS17280 0.81 +3.3 6,557 - EX31_RS17280 0.81 -1.5 6,558 + EX31_RS17280 0.82 -0.4 6,558 + EX31_RS17280 0.82 -1.4 6,559 - EX31_RS17280 0.82 +0.5 6,559 - EX31_RS17280 0.82 +0.7 6,559 - EX31_RS17280 0.82 -0.1 6,559 - EX31_RS17280 0.82 +0.7 6,588 + EX31_RS17280 0.89 -1.3 6,588 + EX31_RS17280 0.89 -0.9 6,588 + EX31_RS17280 0.89 -0.4 6,588 + EX31_RS17280 0.89 +2.1 6,589 - EX31_RS17280 0.89 +1.3 6,589 - EX31_RS17280 0.89 +0.3 6,591 + -2.4 6,591 + +0.6 6,591 + +0.2 6,591 + +0.7 6,591 + +0.5 6,592 - +2.7 6,592 - +1.0 6,595 + +0.1 6,595 + +0.1 6,596 - +0.1 6,596 - -0.4 6,596 - -0.4 6,596 - -0.3 6,691 + -0.1 6,707 + +0.5 6,708 - -0.9 6,736 - -1.9 6,799 + -0.1 6,799 + +0.4 6,799 + +2.5 6,800 - -0.9 6,800 - +0.1 6,800 - +0.1 6,800 - +0.4 6,801 + -1.7 6,801 + -1.4 6,801 + -0.9 6,801 + +0.3 6,801 + +1.7 6,802 - +0.1 6,802 - +0.5 6,802 - +0.7 6,802 - -0.3 6,805 + -0.9 6,805 + +0.3 6,805 + +0.1 6,805 + -1.4 6,805 + -1.3 6,806 - -0.5 6,806 - -1.1 6,806 - -0.1 6,806 - +1.1 6,892 + EX31_RS17285 0.17 +0.6 6,947 + EX31_RS17285 0.24 -1.7 6,947 + EX31_RS17285 0.24 +1.7 6,947 + EX31_RS17285 0.24 -0.9 6,947 + EX31_RS17285 0.24 -0.1 6,948 - EX31_RS17285 0.24 +1.4 6,994 + EX31_RS17285 0.31 -0.1 7,000 + EX31_RS17285 0.31 +0.1 7,152 + EX31_RS17285 0.52 +1.1 7,152 + EX31_RS17285 0.52 -0.6 7,217 + EX31_RS17285 0.60 +0.4 7,217 + EX31_RS17285 0.60 -1.1 7,218 - EX31_RS17285 0.61 -2.5 7,303 + EX31_RS17285 0.72 -0.4 7,381 + EX31_RS17285 0.83 +1.2 7,382 - EX31_RS17285 0.83 -1.7 7,553 + +0.4 7,569 + -1.7 7,570 - -0.3 7,758 + EX31_RS17290 0.26 +0.1 7,761 + EX31_RS17290 0.26 +0.1 7,761 + EX31_RS17290 0.26 -0.3 7,761 + EX31_RS17290 0.26 +0.7 7,762 - EX31_RS17290 0.26 -1.6 7,886 + EX31_RS17290 0.39 -1.3 8,001 + EX31_RS17290 0.51 -0.7 8,123 + EX31_RS17290 0.63 -0.9 8,124 - EX31_RS17290 0.63 -1.6 8,124 - EX31_RS17290 0.63 +0.4 8,199 + EX31_RS17290 0.71 -2.5 8,250 + EX31_RS17290 0.76 -0.4 8,250 + EX31_RS17290 0.76 -1.6 8,250 + EX31_RS17290 0.76 +1.3 8,251 - EX31_RS17290 0.77 -0.9 8,254 + EX31_RS17290 0.77 -0.9 8,254 + EX31_RS17290 0.77 -0.3 8,319 + EX31_RS17290 0.84 +3.3 8,424 + +0.1 8,424 + -1.1 8,477 - +0.2 8,537 - +1.1 8,565 - +0.1 8,577 - -0.7 8,649 + -0.9 8,677 - EX31_RS17295 0.13 +1.1 8,746 + EX31_RS17295 0.47 +1.1 8,747 - EX31_RS17295 0.48 +0.4 8,747 - EX31_RS17295 0.48 +1.3 8,750 - EX31_RS17295 0.49 -2.3 8,750 - EX31_RS17295 0.49 +1.2 8,811 + EX31_RS17295 0.79 +0.8 8,812 - EX31_RS17295 0.79 +0.1 8,903 + -0.3 8,903 + +0.9 8,904 - +1.6 8,905 + -0.1 8,906 - +0.3 8,937 + -0.1 8,963 - -0.9 9,241 - EX31_RS17300 0.15 -1.4 9,247 - EX31_RS17300 0.17 -0.5 9,247 - EX31_RS17300 0.17 +0.6 9,315 - EX31_RS17300 0.30 -1.1 9,323 + EX31_RS17300 0.32 +0.7 9,323 + EX31_RS17300 0.32 -0.0 9,323 + EX31_RS17300 0.32 -0.8 9,324 - EX31_RS17300 0.32 -0.9 9,324 - EX31_RS17300 0.32 +0.6 9,324 - EX31_RS17300 0.32 -1.6 9,324 - EX31_RS17300 0.32 +1.1 9,441 + EX31_RS17300 0.55 -0.1 9,464 + EX31_RS17300 0.60 +0.3 9,465 - EX31_RS17300 0.60 +0.2 9,465 - EX31_RS17300 0.60 +0.1 9,465 - EX31_RS17300 0.60 +0.8 9,465 - EX31_RS17300 0.60 +1.5
Or see this region's nucleotide sequence