Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12770 and EX31_RS12775 are separated by 208 nucleotides EX31_RS12775 and EX31_RS12780 are separated by 55 nucleotides EX31_RS12780 and EX31_RS12785 are separated by 19 nucleotides
EX31_RS12770: EX31_RS12770 - circularly permuted type 2 ATP-grasp protein, at 227,125 to 228,576
_RS12770
EX31_RS12775: EX31_RS12775 - 2-iminobutanoate/2-iminopropanoate deaminase, at 228,785 to 229,171
_RS12775
EX31_RS12780: EX31_RS12780 - aspartate carbamoyltransferase regulatory subunit, at 229,227 to 229,691
_RS12780
EX31_RS12785: EX31_RS12785 - aspartate carbamoyltransferase, at 229,711 to 230,646
_RS12785
Position (kb) 229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 228.254 kb on + strand, within EX31_RS12770 at 228.254 kb on + strand, within EX31_RS12770 at 228.254 kb on + strand, within EX31_RS12770 at 228.254 kb on + strand, within EX31_RS12770 at 228.254 kb on + strand, within EX31_RS12770 at 228.255 kb on - strand, within EX31_RS12770 at 228.255 kb on - strand, within EX31_RS12770 at 228.255 kb on - strand, within EX31_RS12770 at 228.255 kb on - strand, within EX31_RS12770 at 228.255 kb on - strand, within EX31_RS12770 at 228.255 kb on - strand, within EX31_RS12770 at 228.255 kb on - strand, within EX31_RS12770 at 228.255 kb on - strand, within EX31_RS12770 at 228.258 kb on + strand, within EX31_RS12770 at 228.258 kb on + strand, within EX31_RS12770 at 228.258 kb on + strand, within EX31_RS12770 at 228.258 kb on + strand, within EX31_RS12770 at 228.258 kb on + strand, within EX31_RS12770 at 228.259 kb on - strand, within EX31_RS12770 at 228.259 kb on - strand, within EX31_RS12770 at 228.259 kb on - strand, within EX31_RS12770 at 228.259 kb on - strand, within EX31_RS12770 at 228.337 kb on - strand, within EX31_RS12770 at 228.392 kb on + strand, within EX31_RS12770 at 228.392 kb on + strand, within EX31_RS12770 at 228.399 kb on + strand, within EX31_RS12770 at 228.405 kb on + strand, within EX31_RS12770 at 228.406 kb on - strand, within EX31_RS12770 at 228.491 kb on + strand at 228.500 kb on + strand at 228.501 kb on - strand at 228.638 kb on + strand at 228.638 kb on + strand at 228.638 kb on + strand at 228.639 kb on - strand at 228.639 kb on - strand at 228.639 kb on - strand at 228.696 kb on + strand at 228.696 kb on + strand at 228.700 kb on + strand at 228.700 kb on + strand at 228.700 kb on + strand at 228.700 kb on + strand at 228.700 kb on + strand at 228.700 kb on + strand at 228.700 kb on + strand at 228.701 kb on - strand at 228.701 kb on - strand at 228.701 kb on - strand at 228.701 kb on - strand at 228.701 kb on - strand at 228.701 kb on - strand at 228.701 kb on - strand at 228.701 kb on - strand at 228.703 kb on - strand at 228.703 kb on - strand at 228.703 kb on - strand at 228.900 kb on + strand, within EX31_RS12775 at 228.900 kb on + strand, within EX31_RS12775 at 228.901 kb on - strand, within EX31_RS12775 at 228.969 kb on - strand, within EX31_RS12775 at 229.001 kb on + strand, within EX31_RS12775 at 229.001 kb on + strand, within EX31_RS12775 at 229.096 kb on + strand, within EX31_RS12775 at 229.096 kb on + strand, within EX31_RS12775 at 229.097 kb on - strand, within EX31_RS12775 at 229.097 kb on - strand, within EX31_RS12775 at 229.097 kb on - strand, within EX31_RS12775 at 229.122 kb on + strand, within EX31_RS12775 at 229.122 kb on + strand, within EX31_RS12775 at 229.122 kb on + strand, within EX31_RS12775 at 229.122 kb on + strand, within EX31_RS12775 at 229.123 kb on - strand, within EX31_RS12775 at 229.123 kb on - strand, within EX31_RS12775 at 229.123 kb on - strand, within EX31_RS12775 at 229.123 kb on - strand, within EX31_RS12775 at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.171 kb on + strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.172 kb on - strand at 229.218 kb on + strand at 229.219 kb on - strand at 229.219 kb on - strand at 229.220 kb on + strand at 229.220 kb on + strand at 229.236 kb on - strand at 229.273 kb on + strand at 229.273 kb on + strand at 229.273 kb on + strand at 229.426 kb on + strand, within EX31_RS12780 at 229.427 kb on - strand, within EX31_RS12780 at 229.427 kb on - strand, within EX31_RS12780 at 229.462 kb on + strand, within EX31_RS12780 at 229.462 kb on + strand, within EX31_RS12780 at 229.462 kb on + strand, within EX31_RS12780 at 229.463 kb on - strand, within EX31_RS12780 at 229.587 kb on - strand, within EX31_RS12780 at 229.631 kb on + strand, within EX31_RS12780 at 229.632 kb on - strand, within EX31_RS12780 at 229.646 kb on + strand at 229.712 kb on + strand at 229.713 kb on - strand at 229.713 kb on - strand at 229.713 kb on - strand at 229.790 kb on + strand at 229.800 kb on - strand at 229.800 kb on - strand at 229.809 kb on + strand, within EX31_RS12785 at 229.809 kb on + strand, within EX31_RS12785 at 229.810 kb on - strand, within EX31_RS12785 at 229.810 kb on - strand, within EX31_RS12785 at 229.926 kb on - strand, within EX31_RS12785 at 229.967 kb on + strand, within EX31_RS12785 at 229.967 kb on + strand, within EX31_RS12785 at 229.967 kb on + strand, within EX31_RS12785 at 229.968 kb on - strand, within EX31_RS12785 at 229.968 kb on - strand, within EX31_RS12785 at 230.018 kb on + strand, within EX31_RS12785 at 230.052 kb on + strand, within EX31_RS12785 at 230.052 kb on + strand, within EX31_RS12785 at 230.053 kb on - strand, within EX31_RS12785 at 230.054 kb on + strand, within EX31_RS12785 at 230.054 kb on + strand, within EX31_RS12785 at 230.054 kb on + strand, within EX31_RS12785 at 230.054 kb on + strand, within EX31_RS12785 at 230.054 kb on + strand, within EX31_RS12785 at 230.055 kb on - strand, within EX31_RS12785 at 230.086 kb on - strand, within EX31_RS12785 at 230.090 kb on + strand, within EX31_RS12785 at 230.090 kb on + strand, within EX31_RS12785 at 230.090 kb on + strand, within EX31_RS12785 at 230.091 kb on - strand, within EX31_RS12785 at 230.091 kb on - strand, within EX31_RS12785 at 230.091 kb on - strand, within EX31_RS12785 at 230.091 kb on - strand, within EX31_RS12785 at 230.091 kb on - strand, within EX31_RS12785 at 230.107 kb on - strand, within EX31_RS12785 at 230.107 kb on - strand, within EX31_RS12785 at 230.137 kb on + strand, within EX31_RS12785 at 230.138 kb on - strand, within EX31_RS12785 at 230.138 kb on - strand, within EX31_RS12785 at 230.138 kb on - strand, within EX31_RS12785 at 230.150 kb on + strand, within EX31_RS12785 at 230.178 kb on + strand, within EX31_RS12785 at 230.179 kb on - strand, within EX31_RS12785 at 230.179 kb on - strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.351 kb on + strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.352 kb on - strand, within EX31_RS12785 at 230.611 kb on - strand at 230.668 kb on + strand at 230.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 228,254 + EX31_RS12770 0.78 -0.4 228,254 + EX31_RS12770 0.78 -0.4 228,254 + EX31_RS12770 0.78 +1.4 228,254 + EX31_RS12770 0.78 -0.3 228,254 + EX31_RS12770 0.78 +0.1 228,255 - EX31_RS12770 0.78 -1.2 228,255 - EX31_RS12770 0.78 +0.7 228,255 - EX31_RS12770 0.78 +0.0 228,255 - EX31_RS12770 0.78 +0.3 228,255 - EX31_RS12770 0.78 +0.5 228,255 - EX31_RS12770 0.78 -0.2 228,255 - EX31_RS12770 0.78 +0.1 228,255 - EX31_RS12770 0.78 +0.4 228,258 + EX31_RS12770 0.78 -0.0 228,258 + EX31_RS12770 0.78 -1.4 228,258 + EX31_RS12770 0.78 -0.5 228,258 + EX31_RS12770 0.78 +1.5 228,258 + EX31_RS12770 0.78 -0.6 228,259 - EX31_RS12770 0.78 +0.3 228,259 - EX31_RS12770 0.78 -0.3 228,259 - EX31_RS12770 0.78 -1.4 228,259 - EX31_RS12770 0.78 +0.5 228,337 - EX31_RS12770 0.83 +0.9 228,392 + EX31_RS12770 0.87 -1.2 228,392 + EX31_RS12770 0.87 +0.2 228,399 + EX31_RS12770 0.88 +0.7 228,405 + EX31_RS12770 0.88 +1.0 228,406 - EX31_RS12770 0.88 +0.9 228,491 + +2.4 228,500 + +0.2 228,501 - +0.0 228,638 + +1.7 228,638 + +0.7 228,638 + +0.1 228,639 - +0.6 228,639 - +0.8 228,639 - -0.2 228,696 + +0.4 228,696 + -1.6 228,700 + +0.5 228,700 + +0.8 228,700 + -0.3 228,700 + -0.2 228,700 + +1.1 228,700 + +0.9 228,700 + +0.0 228,701 - +0.3 228,701 - +0.5 228,701 - +0.3 228,701 - +1.3 228,701 - +0.4 228,701 - -1.0 228,701 - -0.1 228,701 - +0.3 228,703 - +0.2 228,703 - -1.1 228,703 - +0.2 228,900 + EX31_RS12775 0.30 +1.0 228,900 + EX31_RS12775 0.30 +0.8 228,901 - EX31_RS12775 0.30 +1.0 228,969 - EX31_RS12775 0.48 +0.6 229,001 + EX31_RS12775 0.56 +1.1 229,001 + EX31_RS12775 0.56 -0.9 229,096 + EX31_RS12775 0.80 +0.3 229,096 + EX31_RS12775 0.80 -0.2 229,097 - EX31_RS12775 0.81 -0.3 229,097 - EX31_RS12775 0.81 -1.2 229,097 - EX31_RS12775 0.81 -0.2 229,122 + EX31_RS12775 0.87 -0.5 229,122 + EX31_RS12775 0.87 +0.6 229,122 + EX31_RS12775 0.87 +1.1 229,122 + EX31_RS12775 0.87 +0.1 229,123 - EX31_RS12775 0.87 -0.4 229,123 - EX31_RS12775 0.87 -0.8 229,123 - EX31_RS12775 0.87 +0.8 229,123 - EX31_RS12775 0.87 -0.1 229,171 + -0.2 229,171 + -0.0 229,171 + -0.2 229,171 + -1.1 229,171 + -0.2 229,171 + -1.1 229,171 + +0.3 229,171 + -0.8 229,171 + +0.4 229,171 + +0.0 229,171 + +0.7 229,171 + -0.0 229,172 - +0.2 229,172 - -0.1 229,172 - +0.3 229,172 - +1.4 229,172 - +0.4 229,172 - +0.1 229,172 - -0.4 229,172 - +0.6 229,172 - +0.3 229,172 - -1.0 229,218 + -1.6 229,219 - +0.4 229,219 - -1.5 229,220 + +0.6 229,220 + -0.4 229,236 - -0.8 229,273 + +0.5 229,273 + -0.3 229,273 + -0.3 229,426 + EX31_RS12780 0.43 +1.7 229,427 - EX31_RS12780 0.43 +0.5 229,427 - EX31_RS12780 0.43 +0.4 229,462 + EX31_RS12780 0.51 +0.5 229,462 + EX31_RS12780 0.51 +1.3 229,462 + EX31_RS12780 0.51 -0.2 229,463 - EX31_RS12780 0.51 +0.5 229,587 - EX31_RS12780 0.77 +0.8 229,631 + EX31_RS12780 0.87 +0.7 229,632 - EX31_RS12780 0.87 +0.5 229,646 + +0.1 229,712 + +0.6 229,713 - -1.0 229,713 - -1.0 229,713 - -0.3 229,790 + +0.8 229,800 - -3.0 229,800 - -1.4 229,809 + EX31_RS12785 0.10 -3.0 229,809 + EX31_RS12785 0.10 -1.9 229,810 - EX31_RS12785 0.11 -2.1 229,810 - EX31_RS12785 0.11 -0.4 229,926 - EX31_RS12785 0.23 +0.2 229,967 + EX31_RS12785 0.27 -3.7 229,967 + EX31_RS12785 0.27 -1.3 229,967 + EX31_RS12785 0.27 -1.8 229,968 - EX31_RS12785 0.27 -0.8 229,968 - EX31_RS12785 0.27 +0.1 230,018 + EX31_RS12785 0.33 -3.0 230,052 + EX31_RS12785 0.36 -2.5 230,052 + EX31_RS12785 0.36 -2.1 230,053 - EX31_RS12785 0.37 -0.9 230,054 + EX31_RS12785 0.37 +0.0 230,054 + EX31_RS12785 0.37 -1.6 230,054 + EX31_RS12785 0.37 -0.2 230,054 + EX31_RS12785 0.37 -2.1 230,054 + EX31_RS12785 0.37 -2.1 230,055 - EX31_RS12785 0.37 -2.3 230,086 - EX31_RS12785 0.40 -2.1 230,090 + EX31_RS12785 0.40 -1.1 230,090 + EX31_RS12785 0.40 -0.3 230,090 + EX31_RS12785 0.40 -0.9 230,091 - EX31_RS12785 0.41 -0.5 230,091 - EX31_RS12785 0.41 -2.6 230,091 - EX31_RS12785 0.41 +0.5 230,091 - EX31_RS12785 0.41 -2.4 230,091 - EX31_RS12785 0.41 -1.5 230,107 - EX31_RS12785 0.42 -1.3 230,107 - EX31_RS12785 0.42 -1.4 230,137 + EX31_RS12785 0.46 -1.7 230,138 - EX31_RS12785 0.46 -2.6 230,138 - EX31_RS12785 0.46 -0.7 230,138 - EX31_RS12785 0.46 -1.3 230,150 + EX31_RS12785 0.47 -2.8 230,178 + EX31_RS12785 0.50 -0.8 230,179 - EX31_RS12785 0.50 -2.4 230,179 - EX31_RS12785 0.50 -2.8 230,351 + EX31_RS12785 0.68 -2.4 230,351 + EX31_RS12785 0.68 -2.7 230,351 + EX31_RS12785 0.68 -2.4 230,351 + EX31_RS12785 0.68 -3.4 230,351 + EX31_RS12785 0.68 -1.7 230,351 + EX31_RS12785 0.68 -1.3 230,351 + EX31_RS12785 0.68 -2.2 230,352 - EX31_RS12785 0.68 -1.7 230,352 - EX31_RS12785 0.68 -2.5 230,352 - EX31_RS12785 0.68 -3.6 230,352 - EX31_RS12785 0.68 -2.3 230,352 - EX31_RS12785 0.68 -1.0 230,352 - EX31_RS12785 0.68 -3.1 230,352 - EX31_RS12785 0.68 +0.4 230,352 - EX31_RS12785 0.68 -0.5 230,352 - EX31_RS12785 0.68 -1.9 230,352 - EX31_RS12785 0.68 -3.5 230,611 - -1.4 230,668 + -0.4 230,675 - -0.3
Or see this region's nucleotide sequence