Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12055 and EX31_RS12060 are separated by 85 nucleotides EX31_RS12060 and EX31_RS12065 are separated by 35 nucleotides EX31_RS12065 and EX31_RS12070 are separated by 165 nucleotides
EX31_RS12055: EX31_RS12055 - helix-turn-helix domain-containing protein, at 75,926 to 76,837
_RS12055
EX31_RS12060: EX31_RS12060 - GNAT family N-acetyltransferase, at 76,923 to 77,456
_RS12060
EX31_RS12065: EX31_RS12065 - glutathione S-transferase, at 77,492 to 78,115
_RS12065
EX31_RS12070: EX31_RS12070 - NAD-dependent epimerase/dehydratase family protein, at 78,281 to 79,396
_RS12070
Position (kb) 76
77
78 Strain fitness (log2 ratio) -2
-1
0
1
2 at 75.923 kb on - strand at 75.964 kb on + strand at 75.964 kb on + strand at 75.964 kb on + strand at 75.965 kb on - strand at 75.965 kb on - strand at 75.965 kb on - strand at 75.965 kb on - strand at 75.971 kb on + strand at 75.971 kb on + strand at 75.971 kb on + strand at 75.972 kb on - strand at 76.102 kb on + strand, within EX31_RS12055 at 76.102 kb on + strand, within EX31_RS12055 at 76.102 kb on + strand, within EX31_RS12055 at 76.102 kb on + strand, within EX31_RS12055 at 76.102 kb on + strand, within EX31_RS12055 at 76.102 kb on + strand, within EX31_RS12055 at 76.103 kb on - strand, within EX31_RS12055 at 76.103 kb on - strand, within EX31_RS12055 at 76.103 kb on - strand, within EX31_RS12055 at 76.115 kb on + strand, within EX31_RS12055 at 76.115 kb on + strand, within EX31_RS12055 at 76.115 kb on + strand, within EX31_RS12055 at 76.115 kb on + strand, within EX31_RS12055 at 76.115 kb on + strand, within EX31_RS12055 at 76.116 kb on - strand, within EX31_RS12055 at 76.116 kb on - strand, within EX31_RS12055 at 76.139 kb on + strand, within EX31_RS12055 at 76.140 kb on - strand, within EX31_RS12055 at 76.210 kb on + strand, within EX31_RS12055 at 76.210 kb on + strand, within EX31_RS12055 at 76.210 kb on + strand, within EX31_RS12055 at 76.210 kb on + strand, within EX31_RS12055 at 76.210 kb on + strand, within EX31_RS12055 at 76.211 kb on - strand, within EX31_RS12055 at 76.211 kb on - strand, within EX31_RS12055 at 76.211 kb on - strand, within EX31_RS12055 at 76.256 kb on + strand, within EX31_RS12055 at 76.256 kb on + strand, within EX31_RS12055 at 76.256 kb on + strand, within EX31_RS12055 at 76.257 kb on - strand, within EX31_RS12055 at 76.257 kb on - strand, within EX31_RS12055 at 76.257 kb on - strand, within EX31_RS12055 at 76.360 kb on + strand, within EX31_RS12055 at 76.371 kb on - strand, within EX31_RS12055 at 76.371 kb on - strand, within EX31_RS12055 at 76.469 kb on + strand, within EX31_RS12055 at 76.469 kb on + strand, within EX31_RS12055 at 76.469 kb on + strand, within EX31_RS12055 at 76.469 kb on + strand, within EX31_RS12055 at 76.470 kb on - strand, within EX31_RS12055 at 76.470 kb on - strand, within EX31_RS12055 at 76.470 kb on - strand, within EX31_RS12055 at 76.470 kb on - strand, within EX31_RS12055 at 76.501 kb on - strand, within EX31_RS12055 at 76.522 kb on + strand, within EX31_RS12055 at 76.522 kb on + strand, within EX31_RS12055 at 76.544 kb on + strand, within EX31_RS12055 at 76.544 kb on + strand, within EX31_RS12055 at 76.545 kb on - strand, within EX31_RS12055 at 76.546 kb on + strand, within EX31_RS12055 at 76.547 kb on - strand, within EX31_RS12055 at 76.547 kb on - strand, within EX31_RS12055 at 76.572 kb on + strand, within EX31_RS12055 at 76.575 kb on + strand, within EX31_RS12055 at 76.576 kb on - strand, within EX31_RS12055 at 76.633 kb on + strand, within EX31_RS12055 at 76.634 kb on - strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.664 kb on + strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.665 kb on - strand, within EX31_RS12055 at 76.724 kb on - strand, within EX31_RS12055 at 76.887 kb on + strand at 76.887 kb on + strand at 76.887 kb on + strand at 76.887 kb on + strand at 76.888 kb on - strand at 76.888 kb on - strand at 76.888 kb on - strand at 76.898 kb on + strand at 76.898 kb on + strand at 76.898 kb on + strand at 76.898 kb on + strand at 76.898 kb on + strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.899 kb on - strand at 76.909 kb on - strand at 76.909 kb on - strand at 76.966 kb on + strand at 76.967 kb on - strand at 76.997 kb on + strand, within EX31_RS12060 at 76.997 kb on + strand, within EX31_RS12060 at 76.997 kb on + strand, within EX31_RS12060 at 76.998 kb on - strand, within EX31_RS12060 at 76.998 kb on - strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.034 kb on + strand, within EX31_RS12060 at 77.035 kb on - strand, within EX31_RS12060 at 77.035 kb on - strand, within EX31_RS12060 at 77.121 kb on + strand, within EX31_RS12060 at 77.271 kb on + strand, within EX31_RS12060 at 77.313 kb on - strand, within EX31_RS12060 at 77.313 kb on - strand, within EX31_RS12060 at 77.332 kb on + strand, within EX31_RS12060 at 77.332 kb on + strand, within EX31_RS12060 at 77.332 kb on + strand, within EX31_RS12060 at 77.332 kb on + strand, within EX31_RS12060 at 77.332 kb on + strand, within EX31_RS12060 at 77.333 kb on - strand, within EX31_RS12060 at 77.333 kb on - strand, within EX31_RS12060 at 77.333 kb on - strand, within EX31_RS12060 at 77.333 kb on - strand, within EX31_RS12060 at 77.333 kb on - strand, within EX31_RS12060 at 77.342 kb on + strand, within EX31_RS12060 at 77.342 kb on + strand, within EX31_RS12060 at 77.415 kb on + strand at 77.415 kb on + strand at 77.416 kb on - strand at 77.416 kb on - strand at 77.416 kb on - strand at 77.442 kb on - strand at 77.471 kb on - strand at 77.471 kb on - strand at 77.509 kb on + strand at 77.509 kb on + strand at 77.533 kb on + strand at 77.533 kb on + strand at 77.533 kb on + strand at 77.676 kb on + strand, within EX31_RS12065 at 77.676 kb on + strand, within EX31_RS12065 at 77.676 kb on + strand, within EX31_RS12065 at 77.705 kb on + strand, within EX31_RS12065 at 77.806 kb on + strand, within EX31_RS12065 at 77.807 kb on - strand, within EX31_RS12065 at 77.905 kb on + strand, within EX31_RS12065 at 77.906 kb on - strand, within EX31_RS12065 at 77.945 kb on + strand, within EX31_RS12065 at 77.945 kb on + strand, within EX31_RS12065 at 77.946 kb on - strand, within EX31_RS12065 at 77.946 kb on - strand, within EX31_RS12065 at 77.965 kb on + strand, within EX31_RS12065 at 77.965 kb on + strand, within EX31_RS12065 at 78.053 kb on + strand at 78.053 kb on + strand at 78.053 kb on + strand at 78.054 kb on - strand at 78.057 kb on - strand at 78.092 kb on + strand at 78.093 kb on - strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.189 kb on + strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.190 kb on - strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.193 kb on + strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.194 kb on - strand at 78.197 kb on + strand at 78.197 kb on + strand at 78.197 kb on + strand at 78.197 kb on + strand at 78.198 kb on - strand at 78.198 kb on - strand at 78.198 kb on - strand at 78.198 kb on - strand at 78.283 kb on - strand at 78.344 kb on - strand at 78.344 kb on - strand at 78.344 kb on - strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.351 kb on + strand at 78.352 kb on - strand at 78.352 kb on - strand at 78.352 kb on - strand at 78.359 kb on - strand at 78.390 kb on - strand at 78.390 kb on - strand at 78.390 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 75,923 - -0.2 75,964 + +0.8 75,964 + -0.6 75,964 + -0.6 75,965 - +0.8 75,965 - +1.6 75,965 - +0.1 75,965 - +0.3 75,971 + -0.5 75,971 + -0.6 75,971 + -0.5 75,972 - +0.9 76,102 + EX31_RS12055 0.19 +0.6 76,102 + EX31_RS12055 0.19 -0.9 76,102 + EX31_RS12055 0.19 +0.8 76,102 + EX31_RS12055 0.19 -0.9 76,102 + EX31_RS12055 0.19 +0.2 76,102 + EX31_RS12055 0.19 +0.1 76,103 - EX31_RS12055 0.19 -0.1 76,103 - EX31_RS12055 0.19 -1.3 76,103 - EX31_RS12055 0.19 +0.7 76,115 + EX31_RS12055 0.21 +0.0 76,115 + EX31_RS12055 0.21 +2.1 76,115 + EX31_RS12055 0.21 -0.4 76,115 + EX31_RS12055 0.21 +0.1 76,115 + EX31_RS12055 0.21 +0.0 76,116 - EX31_RS12055 0.21 -0.0 76,116 - EX31_RS12055 0.21 -0.3 76,139 + EX31_RS12055 0.23 -0.3 76,140 - EX31_RS12055 0.23 +0.0 76,210 + EX31_RS12055 0.31 -0.8 76,210 + EX31_RS12055 0.31 +0.2 76,210 + EX31_RS12055 0.31 +0.4 76,210 + EX31_RS12055 0.31 -0.6 76,210 + EX31_RS12055 0.31 +0.5 76,211 - EX31_RS12055 0.31 +1.4 76,211 - EX31_RS12055 0.31 +0.3 76,211 - EX31_RS12055 0.31 +0.0 76,256 + EX31_RS12055 0.36 +0.4 76,256 + EX31_RS12055 0.36 -0.1 76,256 + EX31_RS12055 0.36 +0.5 76,257 - EX31_RS12055 0.36 +0.1 76,257 - EX31_RS12055 0.36 +0.4 76,257 - EX31_RS12055 0.36 -0.0 76,360 + EX31_RS12055 0.48 +0.0 76,371 - EX31_RS12055 0.49 -0.1 76,371 - EX31_RS12055 0.49 -1.3 76,469 + EX31_RS12055 0.60 -0.5 76,469 + EX31_RS12055 0.60 +0.3 76,469 + EX31_RS12055 0.60 +0.1 76,469 + EX31_RS12055 0.60 +0.8 76,470 - EX31_RS12055 0.60 +0.4 76,470 - EX31_RS12055 0.60 +0.3 76,470 - EX31_RS12055 0.60 +1.4 76,470 - EX31_RS12055 0.60 +0.3 76,501 - EX31_RS12055 0.63 +0.5 76,522 + EX31_RS12055 0.65 +0.1 76,522 + EX31_RS12055 0.65 -0.8 76,544 + EX31_RS12055 0.68 +0.9 76,544 + EX31_RS12055 0.68 +0.8 76,545 - EX31_RS12055 0.68 -0.4 76,546 + EX31_RS12055 0.68 +0.4 76,547 - EX31_RS12055 0.68 -0.6 76,547 - EX31_RS12055 0.68 +1.1 76,572 + EX31_RS12055 0.71 -0.3 76,575 + EX31_RS12055 0.71 +0.3 76,576 - EX31_RS12055 0.71 -0.8 76,633 + EX31_RS12055 0.78 +0.3 76,634 - EX31_RS12055 0.78 -0.5 76,664 + EX31_RS12055 0.81 +0.9 76,664 + EX31_RS12055 0.81 -1.1 76,664 + EX31_RS12055 0.81 +0.6 76,664 + EX31_RS12055 0.81 +0.9 76,664 + EX31_RS12055 0.81 +0.9 76,664 + EX31_RS12055 0.81 -1.0 76,664 + EX31_RS12055 0.81 +0.4 76,665 - EX31_RS12055 0.81 +0.3 76,665 - EX31_RS12055 0.81 +0.6 76,665 - EX31_RS12055 0.81 +0.2 76,665 - EX31_RS12055 0.81 -0.3 76,665 - EX31_RS12055 0.81 -0.2 76,665 - EX31_RS12055 0.81 +1.2 76,724 - EX31_RS12055 0.88 -0.5 76,887 + -0.1 76,887 + +0.7 76,887 + -0.0 76,887 + +0.3 76,888 - +0.3 76,888 - +1.0 76,888 - -0.3 76,898 + -0.0 76,898 + +1.2 76,898 + +1.1 76,898 + -0.7 76,898 + -1.5 76,899 - -0.1 76,899 - +0.5 76,899 - -1.6 76,899 - -0.5 76,899 - -0.8 76,899 - -1.9 76,899 - +1.2 76,909 - -0.0 76,909 - -0.5 76,966 + +0.3 76,967 - -0.1 76,997 + EX31_RS12060 0.14 +0.9 76,997 + EX31_RS12060 0.14 +2.4 76,997 + EX31_RS12060 0.14 +0.9 76,998 - EX31_RS12060 0.14 -0.5 76,998 - EX31_RS12060 0.14 -0.4 77,034 + EX31_RS12060 0.21 +0.2 77,034 + EX31_RS12060 0.21 +0.5 77,034 + EX31_RS12060 0.21 -1.7 77,034 + EX31_RS12060 0.21 +0.8 77,034 + EX31_RS12060 0.21 +0.5 77,034 + EX31_RS12060 0.21 -0.4 77,035 - EX31_RS12060 0.21 -0.5 77,035 - EX31_RS12060 0.21 -0.6 77,121 + EX31_RS12060 0.37 +0.5 77,271 + EX31_RS12060 0.65 +0.5 77,313 - EX31_RS12060 0.73 +0.8 77,313 - EX31_RS12060 0.73 +0.4 77,332 + EX31_RS12060 0.77 +1.2 77,332 + EX31_RS12060 0.77 -0.5 77,332 + EX31_RS12060 0.77 -0.3 77,332 + EX31_RS12060 0.77 +1.9 77,332 + EX31_RS12060 0.77 +0.5 77,333 - EX31_RS12060 0.77 -0.7 77,333 - EX31_RS12060 0.77 -0.8 77,333 - EX31_RS12060 0.77 -0.2 77,333 - EX31_RS12060 0.77 -1.0 77,333 - EX31_RS12060 0.77 -0.1 77,342 + EX31_RS12060 0.78 -1.4 77,342 + EX31_RS12060 0.78 +0.7 77,415 + +0.1 77,415 + -0.7 77,416 - +0.9 77,416 - -0.9 77,416 - +0.2 77,442 - -0.6 77,471 - -0.1 77,471 - -0.5 77,509 + +0.3 77,509 + -0.5 77,533 + +0.6 77,533 + -1.5 77,533 + +0.1 77,676 + EX31_RS12065 0.29 +0.2 77,676 + EX31_RS12065 0.29 -0.5 77,676 + EX31_RS12065 0.29 -1.8 77,705 + EX31_RS12065 0.34 -1.5 77,806 + EX31_RS12065 0.50 +0.2 77,807 - EX31_RS12065 0.50 -0.6 77,905 + EX31_RS12065 0.66 -0.6 77,906 - EX31_RS12065 0.66 -0.6 77,945 + EX31_RS12065 0.73 -0.5 77,945 + EX31_RS12065 0.73 +0.4 77,946 - EX31_RS12065 0.73 -0.5 77,946 - EX31_RS12065 0.73 -1.5 77,965 + EX31_RS12065 0.76 -0.0 77,965 + EX31_RS12065 0.76 +1.4 78,053 + -1.0 78,053 + +2.0 78,053 + -0.3 78,054 - -0.8 78,057 - +0.9 78,092 + +0.3 78,093 - -0.2 78,189 + +0.2 78,189 + +0.8 78,189 + -0.1 78,189 + +0.1 78,189 + +0.2 78,189 + +0.2 78,189 + +0.2 78,189 + -0.7 78,190 - +0.5 78,190 - +0.1 78,190 - +0.1 78,190 - -0.7 78,190 - -0.9 78,190 - +0.1 78,190 - -1.8 78,193 + +0.4 78,193 + +0.1 78,193 + -0.3 78,193 + -1.2 78,193 + +0.2 78,193 + -0.4 78,193 + -1.5 78,193 + -0.9 78,193 + +0.2 78,193 + +0.3 78,193 + +0.7 78,193 + +1.4 78,194 - +0.5 78,194 - -0.0 78,194 - +0.1 78,194 - +0.4 78,194 - +0.1 78,194 - +0.2 78,194 - +1.4 78,194 - +1.5 78,194 - +0.2 78,197 + +1.4 78,197 + +1.3 78,197 + -1.3 78,197 + +1.8 78,198 - +0.4 78,198 - -0.2 78,198 - -1.3 78,198 - +0.9 78,283 - -0.2 78,344 - +0.1 78,344 - +0.1 78,344 - +1.3 78,351 + -0.3 78,351 + -0.1 78,351 + -0.0 78,351 + +1.4 78,351 + +0.1 78,351 + +1.3 78,352 - +0.2 78,352 - -0.5 78,352 - -0.6 78,359 - -0.3 78,390 - +0.3 78,390 - -0.1 78,390 - +0.3
Or see this region's nucleotide sequence