Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09565 and EX31_RS09570 are separated by 208 nucleotides EX31_RS09570 and EX31_RS09575 are separated by 7 nucleotides EX31_RS09575 and EX31_RS09580 overlap by 8 nucleotides EX31_RS09580 and EX31_RS09585 overlap by 1 nucleotides EX31_RS09585 and EX31_RS09590 overlap by 4 nucleotides
EX31_RS09565: EX31_RS09565 - flagellar hook-length control protein FliK, at 117,386 to 118,774
_RS09565
EX31_RS09570: EX31_RS09570 - flagellar basal body-associated protein FliL, at 118,983 to 119,483
_RS09570
EX31_RS09575: EX31_RS09575 - flagellar motor switch protein FliM, at 119,491 to 120,513
_RS09575
EX31_RS09580: EX31_RS09580 - flagellar motor switch protein FliN, at 120,506 to 120,922
_RS09580
EX31_RS09585: EX31_RS09585 - flagellar biosynthetic protein FliO, at 120,922 to 121,359
_RS09585
EX31_RS09590: EX31_RS09590 - flagellar type III secretion system pore protein FliP, at 121,356 to 122,138
_RS09590
Position (kb)
119
120
121 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 118.690 kb on + strand at 118.690 kb on + strand at 118.691 kb on - strand at 118.809 kb on + strand at 118.858 kb on + strand at 118.858 kb on + strand at 118.859 kb on - strand at 118.959 kb on + strand at 118.959 kb on + strand at 118.959 kb on + strand at 118.959 kb on + strand at 118.959 kb on + strand at 118.963 kb on + strand at 119.047 kb on + strand, within EX31_RS09570 at 119.047 kb on + strand, within EX31_RS09570 at 119.186 kb on + strand, within EX31_RS09570 at 119.238 kb on + strand, within EX31_RS09570 at 119.238 kb on + strand, within EX31_RS09570 at 119.238 kb on + strand, within EX31_RS09570 at 119.238 kb on + strand, within EX31_RS09570 at 119.238 kb on + strand, within EX31_RS09570 at 119.239 kb on - strand, within EX31_RS09570 at 119.239 kb on - strand, within EX31_RS09570 at 119.239 kb on - strand, within EX31_RS09570 at 119.282 kb on + strand, within EX31_RS09570 at 119.296 kb on - strand, within EX31_RS09570 at 119.490 kb on + strand at 119.491 kb on - strand at 119.504 kb on + strand at 119.504 kb on + strand at 119.504 kb on + strand at 119.504 kb on + strand at 119.504 kb on + strand at 119.505 kb on - strand at 119.609 kb on + strand, within EX31_RS09575 at 119.748 kb on - strand, within EX31_RS09575 at 119.878 kb on - strand, within EX31_RS09575 at 120.022 kb on + strand, within EX31_RS09575 at 120.022 kb on + strand, within EX31_RS09575 at 120.040 kb on + strand, within EX31_RS09575 at 120.072 kb on + strand, within EX31_RS09575 at 120.159 kb on + strand, within EX31_RS09575 at 120.159 kb on + strand, within EX31_RS09575 at 120.159 kb on + strand, within EX31_RS09575 at 120.194 kb on + strand, within EX31_RS09575 at 120.194 kb on + strand, within EX31_RS09575 at 120.225 kb on + strand, within EX31_RS09575 at 120.225 kb on + strand, within EX31_RS09575 at 120.226 kb on - strand, within EX31_RS09575 at 120.353 kb on + strand, within EX31_RS09575 at 120.353 kb on + strand, within EX31_RS09575 at 120.353 kb on + strand, within EX31_RS09575 at 120.354 kb on - strand, within EX31_RS09575 at 120.354 kb on - strand, within EX31_RS09575 at 120.431 kb on + strand at 120.431 kb on + strand at 120.442 kb on + strand at 120.488 kb on + strand at 120.488 kb on + strand at 120.489 kb on - strand at 120.520 kb on + strand at 120.722 kb on - strand, within EX31_RS09580 at 120.860 kb on + strand, within EX31_RS09580 at 120.861 kb on - strand, within EX31_RS09580 at 120.901 kb on + strand at 120.901 kb on + strand at 120.901 kb on + strand at 120.902 kb on - strand at 120.952 kb on + strand at 120.952 kb on + strand at 121.035 kb on + strand, within EX31_RS09585 at 121.148 kb on + strand, within EX31_RS09585 at 121.243 kb on - strand, within EX31_RS09585 at 121.435 kb on + strand, within EX31_RS09590
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 118,690 + +0.4 118,690 + +1.9 118,691 - +1.7 118,809 + -1.8 118,858 + +0.9 118,858 + -0.6 118,859 - +1.3 118,959 + +0.2 118,959 + +0.3 118,959 + +1.0 118,959 + +0.9 118,959 + -1.2 118,963 + -0.1 119,047 + EX31_RS09570 0.13 +1.8 119,047 + EX31_RS09570 0.13 -0.0 119,186 + EX31_RS09570 0.41 +0.2 119,238 + EX31_RS09570 0.51 +0.8 119,238 + EX31_RS09570 0.51 +0.8 119,238 + EX31_RS09570 0.51 -0.5 119,238 + EX31_RS09570 0.51 -0.1 119,238 + EX31_RS09570 0.51 +0.3 119,239 - EX31_RS09570 0.51 +0.2 119,239 - EX31_RS09570 0.51 +3.6 119,239 - EX31_RS09570 0.51 -1.4 119,282 + EX31_RS09570 0.60 -2.0 119,296 - EX31_RS09570 0.62 +0.6 119,490 + +0.8 119,491 - +0.9 119,504 + +0.9 119,504 + -1.1 119,504 + +1.0 119,504 + +1.0 119,504 + +1.0 119,505 - +1.6 119,609 + EX31_RS09575 0.12 +0.9 119,748 - EX31_RS09575 0.25 +2.0 119,878 - EX31_RS09575 0.38 -0.4 120,022 + EX31_RS09575 0.52 +1.1 120,022 + EX31_RS09575 0.52 +0.5 120,040 + EX31_RS09575 0.54 +0.4 120,072 + EX31_RS09575 0.57 +1.8 120,159 + EX31_RS09575 0.65 +1.8 120,159 + EX31_RS09575 0.65 +1.6 120,159 + EX31_RS09575 0.65 +1.0 120,194 + EX31_RS09575 0.69 +0.3 120,194 + EX31_RS09575 0.69 +1.7 120,225 + EX31_RS09575 0.72 +0.7 120,225 + EX31_RS09575 0.72 +1.0 120,226 - EX31_RS09575 0.72 +0.1 120,353 + EX31_RS09575 0.84 +0.2 120,353 + EX31_RS09575 0.84 +0.7 120,353 + EX31_RS09575 0.84 +0.6 120,354 - EX31_RS09575 0.84 +1.6 120,354 - EX31_RS09575 0.84 +0.6 120,431 + +0.6 120,431 + -0.6 120,442 + -0.1 120,488 + +0.5 120,488 + +0.7 120,489 - +2.9 120,520 + +1.1 120,722 - EX31_RS09580 0.52 +0.8 120,860 + EX31_RS09580 0.85 +1.1 120,861 - EX31_RS09580 0.85 +0.6 120,901 + +1.8 120,901 + +1.5 120,901 + +0.7 120,902 - +0.0 120,952 + -0.4 120,952 + +1.4 121,035 + EX31_RS09585 0.26 +0.2 121,148 + EX31_RS09585 0.52 +0.9 121,243 - EX31_RS09585 0.73 +1.8 121,435 + EX31_RS09590 0.10 +0.6
Or see this region's nucleotide sequence