Strain Fitness in Rahnella sp. WP5 around EX31_RS09575

Experiment: L-Serine (N); ShewMM media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS09565 and EX31_RS09570 are separated by 208 nucleotidesEX31_RS09570 and EX31_RS09575 are separated by 7 nucleotidesEX31_RS09575 and EX31_RS09580 overlap by 8 nucleotidesEX31_RS09580 and EX31_RS09585 overlap by 1 nucleotidesEX31_RS09585 and EX31_RS09590 overlap by 4 nucleotides EX31_RS09565: EX31_RS09565 - flagellar hook-length control protein FliK, at 117,386 to 118,774 _RS09565 EX31_RS09570: EX31_RS09570 - flagellar basal body-associated protein FliL, at 118,983 to 119,483 _RS09570 EX31_RS09575: EX31_RS09575 - flagellar motor switch protein FliM, at 119,491 to 120,513 _RS09575 EX31_RS09580: EX31_RS09580 - flagellar motor switch protein FliN, at 120,506 to 120,922 _RS09580 EX31_RS09585: EX31_RS09585 - flagellar biosynthetic protein FliO, at 120,922 to 121,359 _RS09585 EX31_RS09590: EX31_RS09590 - flagellar type III secretion system pore protein FliP, at 121,356 to 122,138 _RS09590 Position (kb) 119 120 121Strain fitness (log2 ratio) -2 -1 0 1 2 3at 118.690 kb on + strandat 118.690 kb on + strandat 118.691 kb on - strandat 118.809 kb on + strandat 118.858 kb on + strandat 118.858 kb on + strandat 118.859 kb on - strandat 118.959 kb on + strandat 118.959 kb on + strandat 118.959 kb on + strandat 118.959 kb on + strandat 118.959 kb on + strandat 118.963 kb on + strandat 119.047 kb on + strand, within EX31_RS09570at 119.047 kb on + strand, within EX31_RS09570at 119.186 kb on + strand, within EX31_RS09570at 119.238 kb on + strand, within EX31_RS09570at 119.238 kb on + strand, within EX31_RS09570at 119.238 kb on + strand, within EX31_RS09570at 119.238 kb on + strand, within EX31_RS09570at 119.238 kb on + strand, within EX31_RS09570at 119.239 kb on - strand, within EX31_RS09570at 119.239 kb on - strand, within EX31_RS09570at 119.239 kb on - strand, within EX31_RS09570at 119.282 kb on + strand, within EX31_RS09570at 119.296 kb on - strand, within EX31_RS09570at 119.490 kb on + strandat 119.491 kb on - strandat 119.504 kb on + strandat 119.504 kb on + strandat 119.504 kb on + strandat 119.504 kb on + strandat 119.504 kb on + strandat 119.505 kb on - strandat 119.609 kb on + strand, within EX31_RS09575at 119.748 kb on - strand, within EX31_RS09575at 119.878 kb on - strand, within EX31_RS09575at 120.022 kb on + strand, within EX31_RS09575at 120.022 kb on + strand, within EX31_RS09575at 120.040 kb on + strand, within EX31_RS09575at 120.072 kb on + strand, within EX31_RS09575at 120.159 kb on + strand, within EX31_RS09575at 120.159 kb on + strand, within EX31_RS09575at 120.159 kb on + strand, within EX31_RS09575at 120.194 kb on + strand, within EX31_RS09575at 120.194 kb on + strand, within EX31_RS09575at 120.225 kb on + strand, within EX31_RS09575at 120.225 kb on + strand, within EX31_RS09575at 120.226 kb on - strand, within EX31_RS09575at 120.353 kb on + strand, within EX31_RS09575at 120.353 kb on + strand, within EX31_RS09575at 120.353 kb on + strand, within EX31_RS09575at 120.354 kb on - strand, within EX31_RS09575at 120.354 kb on - strand, within EX31_RS09575at 120.431 kb on + strandat 120.431 kb on + strandat 120.442 kb on + strandat 120.488 kb on + strandat 120.488 kb on + strandat 120.489 kb on - strandat 120.520 kb on + strandat 120.722 kb on - strand, within EX31_RS09580at 120.860 kb on + strand, within EX31_RS09580at 120.861 kb on - strand, within EX31_RS09580at 120.901 kb on + strandat 120.901 kb on + strandat 120.901 kb on + strandat 120.902 kb on - strandat 120.952 kb on + strandat 120.952 kb on + strandat 121.035 kb on + strand, within EX31_RS09585at 121.148 kb on + strand, within EX31_RS09585at 121.243 kb on - strand, within EX31_RS09585at 121.435 kb on + strand, within EX31_RS09590

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking
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118,690 + +0.4
118,690 + +1.9
118,691 - +1.7
118,809 + -1.8
118,858 + +0.9
118,858 + -0.6
118,859 - +1.3
118,959 + +0.2
118,959 + +0.3
118,959 + +1.0
118,959 + +0.9
118,959 + -1.2
118,963 + -0.1
119,047 + EX31_RS09570 0.13 +1.8
119,047 + EX31_RS09570 0.13 -0.0
119,186 + EX31_RS09570 0.41 +0.2
119,238 + EX31_RS09570 0.51 +0.8
119,238 + EX31_RS09570 0.51 +0.8
119,238 + EX31_RS09570 0.51 -0.5
119,238 + EX31_RS09570 0.51 -0.1
119,238 + EX31_RS09570 0.51 +0.3
119,239 - EX31_RS09570 0.51 +0.2
119,239 - EX31_RS09570 0.51 +3.6
119,239 - EX31_RS09570 0.51 -1.4
119,282 + EX31_RS09570 0.60 -2.0
119,296 - EX31_RS09570 0.62 +0.6
119,490 + +0.8
119,491 - +0.9
119,504 + +0.9
119,504 + -1.1
119,504 + +1.0
119,504 + +1.0
119,504 + +1.0
119,505 - +1.6
119,609 + EX31_RS09575 0.12 +0.9
119,748 - EX31_RS09575 0.25 +2.0
119,878 - EX31_RS09575 0.38 -0.4
120,022 + EX31_RS09575 0.52 +1.1
120,022 + EX31_RS09575 0.52 +0.5
120,040 + EX31_RS09575 0.54 +0.4
120,072 + EX31_RS09575 0.57 +1.8
120,159 + EX31_RS09575 0.65 +1.8
120,159 + EX31_RS09575 0.65 +1.6
120,159 + EX31_RS09575 0.65 +1.0
120,194 + EX31_RS09575 0.69 +0.3
120,194 + EX31_RS09575 0.69 +1.7
120,225 + EX31_RS09575 0.72 +0.7
120,225 + EX31_RS09575 0.72 +1.0
120,226 - EX31_RS09575 0.72 +0.1
120,353 + EX31_RS09575 0.84 +0.2
120,353 + EX31_RS09575 0.84 +0.7
120,353 + EX31_RS09575 0.84 +0.6
120,354 - EX31_RS09575 0.84 +1.6
120,354 - EX31_RS09575 0.84 +0.6
120,431 + +0.6
120,431 + -0.6
120,442 + -0.1
120,488 + +0.5
120,488 + +0.7
120,489 - +2.9
120,520 + +1.1
120,722 - EX31_RS09580 0.52 +0.8
120,860 + EX31_RS09580 0.85 +1.1
120,861 - EX31_RS09580 0.85 +0.6
120,901 + +1.8
120,901 + +1.5
120,901 + +0.7
120,902 - +0.0
120,952 + -0.4
120,952 + +1.4
121,035 + EX31_RS09585 0.26 +0.2
121,148 + EX31_RS09585 0.52 +0.9
121,243 - EX31_RS09585 0.73 +1.8
121,435 + EX31_RS09590 0.10 +0.6

Or see this region's nucleotide sequence