Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09450 and EX31_RS09455 are separated by 308 nucleotides EX31_RS09455 and EX31_RS09460 are separated by 375 nucleotides
EX31_RS09450: EX31_RS09450 - RNA polymerase sigma factor FliA, at 99,313 to 100,035
_RS09450
EX31_RS09455: EX31_RS09455 - FliC/FljB family flagellin, at 100,344 to 101,624
_RS09455
EX31_RS09460: EX31_RS09460 - flagellar filament capping protein FliD, at 102,000 to 103,436
_RS09460
Position (kb) 100
101
102 Strain fitness (log2 ratio) -2
-1
0
1
2 at 99.349 kb on - strand at 99.374 kb on - strand at 99.374 kb on - strand at 99.374 kb on - strand at 99.438 kb on - strand, within EX31_RS09450 at 99.438 kb on - strand, within EX31_RS09450 at 99.438 kb on - strand, within EX31_RS09450 at 99.438 kb on - strand, within EX31_RS09450 at 99.445 kb on - strand, within EX31_RS09450 at 99.445 kb on - strand, within EX31_RS09450 at 99.445 kb on - strand, within EX31_RS09450 at 99.445 kb on - strand, within EX31_RS09450 at 99.445 kb on - strand, within EX31_RS09450 at 99.448 kb on + strand, within EX31_RS09450 at 99.449 kb on - strand, within EX31_RS09450 at 99.449 kb on - strand, within EX31_RS09450 at 99.449 kb on - strand, within EX31_RS09450 at 99.449 kb on - strand, within EX31_RS09450 at 99.449 kb on - strand, within EX31_RS09450 at 99.508 kb on - strand, within EX31_RS09450 at 99.508 kb on - strand, within EX31_RS09450 at 99.508 kb on - strand, within EX31_RS09450 at 99.527 kb on - strand, within EX31_RS09450 at 99.527 kb on - strand, within EX31_RS09450 at 99.599 kb on + strand, within EX31_RS09450 at 99.615 kb on + strand, within EX31_RS09450 at 99.616 kb on - strand, within EX31_RS09450 at 99.624 kb on + strand, within EX31_RS09450 at 99.632 kb on - strand, within EX31_RS09450 at 99.632 kb on - strand, within EX31_RS09450 at 99.635 kb on - strand, within EX31_RS09450 at 99.635 kb on - strand, within EX31_RS09450 at 99.635 kb on - strand, within EX31_RS09450 at 99.751 kb on - strand, within EX31_RS09450 at 99.751 kb on - strand, within EX31_RS09450 at 99.813 kb on + strand, within EX31_RS09450 at 99.827 kb on + strand, within EX31_RS09450 at 99.828 kb on - strand, within EX31_RS09450 at 99.828 kb on - strand, within EX31_RS09450 at 99.828 kb on - strand, within EX31_RS09450 at 99.877 kb on + strand, within EX31_RS09450 at 99.878 kb on - strand, within EX31_RS09450 at 99.905 kb on - strand, within EX31_RS09450 at 99.905 kb on - strand, within EX31_RS09450 at 99.978 kb on - strand at 99.978 kb on - strand at 100.020 kb on + strand at 100.023 kb on - strand at 100.023 kb on - strand at 100.023 kb on - strand at 100.023 kb on - strand at 100.023 kb on - strand at 100.023 kb on - strand at 100.042 kb on - strand at 100.104 kb on + strand at 100.105 kb on - strand at 100.134 kb on - strand at 100.134 kb on - strand at 100.225 kb on - strand at 100.247 kb on - strand at 100.247 kb on - strand at 100.247 kb on - strand at 100.247 kb on - strand at 100.325 kb on - strand at 100.346 kb on - strand at 100.346 kb on - strand at 100.346 kb on - strand at 100.362 kb on + strand at 100.382 kb on + strand at 100.382 kb on + strand at 100.383 kb on - strand at 100.389 kb on - strand at 100.389 kb on - strand at 100.389 kb on - strand at 100.403 kb on + strand at 100.404 kb on - strand at 100.404 kb on - strand at 100.473 kb on - strand, within EX31_RS09455 at 100.473 kb on - strand, within EX31_RS09455 at 100.473 kb on - strand, within EX31_RS09455 at 100.580 kb on + strand, within EX31_RS09455 at 100.633 kb on + strand, within EX31_RS09455 at 100.634 kb on - strand, within EX31_RS09455 at 100.634 kb on - strand, within EX31_RS09455 at 100.743 kb on - strand, within EX31_RS09455 at 100.759 kb on + strand, within EX31_RS09455 at 100.759 kb on + strand, within EX31_RS09455 at 100.759 kb on + strand, within EX31_RS09455 at 100.817 kb on + strand, within EX31_RS09455 at 100.818 kb on - strand, within EX31_RS09455 at 100.818 kb on - strand, within EX31_RS09455 at 100.849 kb on + strand, within EX31_RS09455 at 100.876 kb on - strand, within EX31_RS09455 at 100.896 kb on + strand, within EX31_RS09455 at 100.909 kb on + strand, within EX31_RS09455 at 100.910 kb on - strand, within EX31_RS09455 at 100.910 kb on - strand, within EX31_RS09455 at 100.936 kb on + strand, within EX31_RS09455 at 100.936 kb on + strand, within EX31_RS09455 at 100.936 kb on + strand, within EX31_RS09455 at 100.936 kb on + strand, within EX31_RS09455 at 100.937 kb on - strand, within EX31_RS09455 at 101.130 kb on - strand, within EX31_RS09455 at 101.160 kb on - strand, within EX31_RS09455 at 101.215 kb on - strand, within EX31_RS09455 at 101.215 kb on - strand, within EX31_RS09455 at 101.248 kb on - strand, within EX31_RS09455 at 101.248 kb on - strand, within EX31_RS09455 at 101.352 kb on - strand, within EX31_RS09455 at 101.358 kb on + strand, within EX31_RS09455 at 101.358 kb on + strand, within EX31_RS09455 at 101.359 kb on - strand, within EX31_RS09455 at 101.359 kb on - strand, within EX31_RS09455 at 101.359 kb on - strand, within EX31_RS09455 at 101.359 kb on - strand, within EX31_RS09455 at 101.359 kb on - strand, within EX31_RS09455 at 101.411 kb on + strand, within EX31_RS09455 at 101.412 kb on - strand, within EX31_RS09455 at 101.456 kb on + strand, within EX31_RS09455 at 101.457 kb on - strand, within EX31_RS09455 at 101.612 kb on + strand at 101.613 kb on - strand at 101.630 kb on + strand at 101.630 kb on + strand at 101.696 kb on + strand at 101.726 kb on + strand at 101.727 kb on - strand at 101.751 kb on + strand at 101.764 kb on - strand at 101.774 kb on - strand at 101.774 kb on - strand at 101.774 kb on - strand at 101.795 kb on - strand at 101.811 kb on + strand at 101.811 kb on + strand at 101.857 kb on + strand at 101.857 kb on + strand at 101.858 kb on - strand at 101.858 kb on - strand at 101.858 kb on - strand at 101.859 kb on + strand at 101.860 kb on - strand at 101.863 kb on + strand at 101.863 kb on + strand at 101.863 kb on + strand at 101.863 kb on + strand at 101.958 kb on + strand at 101.958 kb on + strand at 101.959 kb on - strand at 101.968 kb on + strand at 101.968 kb on + strand at 101.968 kb on + strand at 101.968 kb on + strand at 101.968 kb on + strand at 101.995 kb on + strand at 102.000 kb on - strand at 102.018 kb on - strand at 102.028 kb on + strand at 102.028 kb on + strand at 102.028 kb on + strand at 102.028 kb on + strand at 102.029 kb on - strand at 102.046 kb on + strand at 102.126 kb on + strand at 102.212 kb on + strand, within EX31_RS09460 at 102.212 kb on + strand, within EX31_RS09460 at 102.294 kb on + strand, within EX31_RS09460 at 102.294 kb on + strand, within EX31_RS09460 at 102.389 kb on + strand, within EX31_RS09460 at 102.546 kb on + strand, within EX31_RS09460
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 99,349 - -0.2 99,374 - +0.6 99,374 - -0.6 99,374 - +0.1 99,438 - EX31_RS09450 0.17 +1.4 99,438 - EX31_RS09450 0.17 -1.3 99,438 - EX31_RS09450 0.17 -0.0 99,438 - EX31_RS09450 0.17 +0.8 99,445 - EX31_RS09450 0.18 +0.4 99,445 - EX31_RS09450 0.18 +0.8 99,445 - EX31_RS09450 0.18 +0.9 99,445 - EX31_RS09450 0.18 +1.7 99,445 - EX31_RS09450 0.18 -0.2 99,448 + EX31_RS09450 0.19 -0.2 99,449 - EX31_RS09450 0.19 +0.3 99,449 - EX31_RS09450 0.19 +0.2 99,449 - EX31_RS09450 0.19 -1.4 99,449 - EX31_RS09450 0.19 -0.0 99,449 - EX31_RS09450 0.19 -0.6 99,508 - EX31_RS09450 0.27 +1.1 99,508 - EX31_RS09450 0.27 +0.6 99,508 - EX31_RS09450 0.27 +2.0 99,527 - EX31_RS09450 0.30 +0.6 99,527 - EX31_RS09450 0.30 +0.8 99,599 + EX31_RS09450 0.40 -0.9 99,615 + EX31_RS09450 0.42 +0.6 99,616 - EX31_RS09450 0.42 +0.6 99,624 + EX31_RS09450 0.43 +0.3 99,632 - EX31_RS09450 0.44 +0.8 99,632 - EX31_RS09450 0.44 +0.8 99,635 - EX31_RS09450 0.45 -0.4 99,635 - EX31_RS09450 0.45 +1.2 99,635 - EX31_RS09450 0.45 +1.1 99,751 - EX31_RS09450 0.61 +0.6 99,751 - EX31_RS09450 0.61 +1.1 99,813 + EX31_RS09450 0.69 -0.1 99,827 + EX31_RS09450 0.71 -0.0 99,828 - EX31_RS09450 0.71 +0.8 99,828 - EX31_RS09450 0.71 +0.3 99,828 - EX31_RS09450 0.71 +0.2 99,877 + EX31_RS09450 0.78 -0.4 99,878 - EX31_RS09450 0.78 +1.2 99,905 - EX31_RS09450 0.82 +1.3 99,905 - EX31_RS09450 0.82 +1.5 99,978 - +0.6 99,978 - +0.3 100,020 + +0.3 100,023 - +1.2 100,023 - +0.9 100,023 - -1.2 100,023 - +0.6 100,023 - +1.4 100,023 - +0.3 100,042 - +0.2 100,104 + +0.6 100,105 - +0.6 100,134 - -0.3 100,134 - -0.6 100,225 - -1.7 100,247 - -0.9 100,247 - +1.8 100,247 - +0.0 100,247 - +0.6 100,325 - -0.0 100,346 - +1.0 100,346 - +1.1 100,346 - +0.8 100,362 + +0.3 100,382 + +0.9 100,382 + +1.3 100,383 - -0.0 100,389 - +0.9 100,389 - +1.6 100,389 - +2.1 100,403 + +1.7 100,404 - +0.5 100,404 - +1.4 100,473 - EX31_RS09455 0.10 +0.2 100,473 - EX31_RS09455 0.10 +1.2 100,473 - EX31_RS09455 0.10 +1.7 100,580 + EX31_RS09455 0.18 +1.0 100,633 + EX31_RS09455 0.23 -0.6 100,634 - EX31_RS09455 0.23 +1.6 100,634 - EX31_RS09455 0.23 +0.5 100,743 - EX31_RS09455 0.31 +0.6 100,759 + EX31_RS09455 0.32 -1.6 100,759 + EX31_RS09455 0.32 +0.1 100,759 + EX31_RS09455 0.32 -1.1 100,817 + EX31_RS09455 0.37 +1.2 100,818 - EX31_RS09455 0.37 +2.0 100,818 - EX31_RS09455 0.37 +1.1 100,849 + EX31_RS09455 0.39 -0.0 100,876 - EX31_RS09455 0.42 +1.0 100,896 + EX31_RS09455 0.43 -0.8 100,909 + EX31_RS09455 0.44 +0.7 100,910 - EX31_RS09455 0.44 +2.4 100,910 - EX31_RS09455 0.44 +1.0 100,936 + EX31_RS09455 0.46 +1.2 100,936 + EX31_RS09455 0.46 +1.1 100,936 + EX31_RS09455 0.46 +2.1 100,936 + EX31_RS09455 0.46 +1.3 100,937 - EX31_RS09455 0.46 -0.0 101,130 - EX31_RS09455 0.61 +2.2 101,160 - EX31_RS09455 0.64 +0.6 101,215 - EX31_RS09455 0.68 +2.6 101,215 - EX31_RS09455 0.68 +1.6 101,248 - EX31_RS09455 0.71 +0.6 101,248 - EX31_RS09455 0.71 +0.7 101,352 - EX31_RS09455 0.79 +2.1 101,358 + EX31_RS09455 0.79 +0.4 101,358 + EX31_RS09455 0.79 +1.4 101,359 - EX31_RS09455 0.79 +0.3 101,359 - EX31_RS09455 0.79 +0.3 101,359 - EX31_RS09455 0.79 +2.3 101,359 - EX31_RS09455 0.79 -1.0 101,359 - EX31_RS09455 0.79 +1.6 101,411 + EX31_RS09455 0.83 +0.2 101,412 - EX31_RS09455 0.83 +0.5 101,456 + EX31_RS09455 0.87 +1.6 101,457 - EX31_RS09455 0.87 +0.8 101,612 + +0.7 101,613 - +0.8 101,630 + +0.8 101,630 + +0.6 101,696 + -0.2 101,726 + +1.5 101,727 - +0.8 101,751 + +0.1 101,764 - +1.6 101,774 - -1.9 101,774 - -0.3 101,774 - +0.0 101,795 - -0.8 101,811 + +0.6 101,811 + -0.0 101,857 + +0.1 101,857 + -1.3 101,858 - +0.8 101,858 - +1.8 101,858 - -0.0 101,859 + +0.6 101,860 - -0.1 101,863 + +0.4 101,863 + -0.2 101,863 + +0.4 101,863 + +1.1 101,958 + +1.1 101,958 + +0.4 101,959 - -0.4 101,968 + -0.0 101,968 + +0.4 101,968 + +0.5 101,968 + +0.1 101,968 + -0.3 101,995 + +0.8 102,000 - +1.7 102,018 - +0.1 102,028 + +0.7 102,028 + +0.4 102,028 + -1.2 102,028 + +1.6 102,029 - -1.2 102,046 + +0.8 102,126 + +0.6 102,212 + EX31_RS09460 0.15 -0.4 102,212 + EX31_RS09460 0.15 -0.7 102,294 + EX31_RS09460 0.20 -1.0 102,294 + EX31_RS09460 0.20 +2.3 102,389 + EX31_RS09460 0.27 +1.6 102,546 + EX31_RS09460 0.38 +0.4
Or see this region's nucleotide sequence