Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02955 and EX31_RS02960 are separated by 74 nucleotides EX31_RS02960 and EX31_RS02965 are separated by 288 nucleotides EX31_RS02965 and EX31_RS02970 are separated by 74 nucleotides
EX31_RS02955: EX31_RS02955 - YjbF family lipoprotein, at 71,374 to 72,030
_RS02955
EX31_RS02960: EX31_RS02960 - hypothetical protein, at 72,105 to 72,320
_RS02960
EX31_RS02965: EX31_RS02965 - EAL domain-containing protein, at 72,609 to 73,295
_RS02965
EX31_RS02970: EX31_RS02970 - helix-turn-helix transcriptional regulator, at 73,370 to 73,966
_RS02970
Position (kb) 72
73
74 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 71.784 kb on + strand, within EX31_RS02955 at 71.785 kb on - strand, within EX31_RS02955 at 71.785 kb on - strand, within EX31_RS02955 at 71.789 kb on - strand, within EX31_RS02955 at 71.789 kb on - strand, within EX31_RS02955 at 71.834 kb on + strand, within EX31_RS02955 at 71.862 kb on + strand, within EX31_RS02955 at 71.863 kb on - strand, within EX31_RS02955 at 71.863 kb on - strand, within EX31_RS02955 at 71.876 kb on + strand, within EX31_RS02955 at 71.895 kb on + strand, within EX31_RS02955 at 71.952 kb on + strand, within EX31_RS02955 at 71.953 kb on - strand, within EX31_RS02955 at 71.995 kb on + strand at 71.996 kb on - strand at 72.042 kb on + strand at 72.042 kb on + strand at 72.043 kb on - strand at 72.045 kb on - strand at 72.053 kb on + strand at 72.054 kb on - strand at 72.054 kb on - strand at 72.094 kb on + strand at 72.104 kb on - strand at 72.104 kb on - strand at 72.107 kb on - strand at 72.134 kb on + strand, within EX31_RS02960 at 72.158 kb on + strand, within EX31_RS02960 at 72.167 kb on + strand, within EX31_RS02960 at 72.168 kb on - strand, within EX31_RS02960 at 72.172 kb on - strand, within EX31_RS02960 at 72.172 kb on - strand, within EX31_RS02960 at 72.172 kb on - strand, within EX31_RS02960 at 72.172 kb on - strand, within EX31_RS02960 at 72.271 kb on + strand, within EX31_RS02960 at 72.271 kb on + strand, within EX31_RS02960 at 72.273 kb on + strand, within EX31_RS02960 at 72.274 kb on - strand, within EX31_RS02960 at 72.324 kb on + strand at 72.381 kb on + strand at 72.382 kb on - strand at 72.400 kb on + strand at 72.422 kb on - strand at 72.425 kb on + strand at 72.425 kb on + strand at 72.430 kb on + strand at 72.434 kb on + strand at 72.477 kb on + strand at 72.519 kb on - strand at 72.564 kb on + strand at 72.569 kb on - strand at 72.581 kb on + strand at 72.625 kb on - strand at 72.641 kb on + strand at 72.668 kb on + strand at 72.690 kb on + strand, within EX31_RS02965 at 72.732 kb on - strand, within EX31_RS02965 at 72.745 kb on + strand, within EX31_RS02965 at 72.746 kb on - strand, within EX31_RS02965 at 72.822 kb on + strand, within EX31_RS02965 at 72.825 kb on - strand, within EX31_RS02965 at 72.826 kb on + strand, within EX31_RS02965 at 72.827 kb on - strand, within EX31_RS02965 at 72.854 kb on + strand, within EX31_RS02965 at 72.909 kb on + strand, within EX31_RS02965 at 72.978 kb on + strand, within EX31_RS02965 at 72.978 kb on + strand, within EX31_RS02965 at 72.978 kb on + strand, within EX31_RS02965 at 72.979 kb on - strand, within EX31_RS02965 at 72.979 kb on - strand, within EX31_RS02965 at 72.979 kb on - strand, within EX31_RS02965 at 72.979 kb on - strand, within EX31_RS02965 at 72.979 kb on - strand, within EX31_RS02965 at 72.980 kb on + strand, within EX31_RS02965 at 72.980 kb on + strand, within EX31_RS02965 at 72.980 kb on + strand, within EX31_RS02965 at 72.980 kb on + strand, within EX31_RS02965 at 72.980 kb on + strand, within EX31_RS02965 at 72.982 kb on + strand, within EX31_RS02965 at 72.982 kb on + strand, within EX31_RS02965 at 72.982 kb on + strand, within EX31_RS02965 at 72.983 kb on - strand, within EX31_RS02965 at 72.983 kb on - strand, within EX31_RS02965 at 73.029 kb on - strand, within EX31_RS02965 at 73.046 kb on - strand, within EX31_RS02965 at 73.088 kb on + strand, within EX31_RS02965 at 73.089 kb on - strand, within EX31_RS02965 at 73.089 kb on - strand, within EX31_RS02965 at 73.089 kb on - strand, within EX31_RS02965 at 73.159 kb on - strand, within EX31_RS02965 at 73.178 kb on - strand, within EX31_RS02965 at 73.227 kb on + strand at 73.228 kb on - strand at 73.315 kb on - strand at 73.499 kb on + strand, within EX31_RS02970 at 73.578 kb on + strand, within EX31_RS02970 at 73.579 kb on - strand, within EX31_RS02970 at 73.709 kb on + strand, within EX31_RS02970 at 73.709 kb on + strand, within EX31_RS02970 at 73.710 kb on - strand, within EX31_RS02970 at 73.714 kb on + strand, within EX31_RS02970 at 73.758 kb on - strand, within EX31_RS02970 at 73.758 kb on - strand, within EX31_RS02970 at 73.758 kb on - strand, within EX31_RS02970 at 73.827 kb on + strand, within EX31_RS02970 at 73.884 kb on - strand, within EX31_RS02970 at 73.887 kb on + strand, within EX31_RS02970 at 73.888 kb on - strand, within EX31_RS02970 at 73.908 kb on - strand at 73.953 kb on - strand at 73.955 kb on + strand at 74.086 kb on + strand at 74.110 kb on - strand at 74.114 kb on - strand at 74.114 kb on - strand at 74.118 kb on + strand at 74.119 kb on - strand at 74.119 kb on - strand at 74.159 kb on + strand at 74.160 kb on - strand at 74.160 kb on - strand at 74.163 kb on - strand at 74.170 kb on - strand at 74.170 kb on - strand at 74.194 kb on + strand at 74.248 kb on - strand at 74.248 kb on - strand at 74.248 kb on - strand at 74.257 kb on + strand at 74.257 kb on + strand at 74.257 kb on + strand at 74.258 kb on - strand at 74.258 kb on - strand at 74.276 kb on + strand at 74.276 kb on + strand at 74.276 kb on + strand at 74.277 kb on - strand at 74.277 kb on - strand at 74.277 kb on - strand at 74.277 kb on - strand at 74.277 kb on - strand at 74.277 kb on - strand at 74.277 kb on - strand at 74.278 kb on + strand at 74.278 kb on + strand at 74.279 kb on - strand at 74.279 kb on - strand at 74.279 kb on - strand at 74.289 kb on + strand at 74.289 kb on + strand at 74.290 kb on - strand at 74.290 kb on - strand at 74.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 71,784 + EX31_RS02955 0.62 -0.8 71,785 - EX31_RS02955 0.63 -1.1 71,785 - EX31_RS02955 0.63 +0.9 71,789 - EX31_RS02955 0.63 -0.2 71,789 - EX31_RS02955 0.63 +1.2 71,834 + EX31_RS02955 0.70 +0.7 71,862 + EX31_RS02955 0.74 -0.7 71,863 - EX31_RS02955 0.74 -0.4 71,863 - EX31_RS02955 0.74 -1.0 71,876 + EX31_RS02955 0.76 -1.0 71,895 + EX31_RS02955 0.79 -0.9 71,952 + EX31_RS02955 0.88 +0.1 71,953 - EX31_RS02955 0.88 -0.4 71,995 + +0.5 71,996 - +0.4 72,042 + -0.3 72,042 + +1.7 72,043 - -1.4 72,045 - -0.2 72,053 + -3.0 72,054 - -0.1 72,054 - -1.7 72,094 + -0.3 72,104 - -0.8 72,104 - -0.3 72,107 - -0.7 72,134 + EX31_RS02960 0.13 +1.4 72,158 + EX31_RS02960 0.25 +1.2 72,167 + EX31_RS02960 0.29 -0.1 72,168 - EX31_RS02960 0.29 -0.3 72,172 - EX31_RS02960 0.31 +0.8 72,172 - EX31_RS02960 0.31 +0.9 72,172 - EX31_RS02960 0.31 +0.2 72,172 - EX31_RS02960 0.31 +1.4 72,271 + EX31_RS02960 0.77 +0.4 72,271 + EX31_RS02960 0.77 +1.3 72,273 + EX31_RS02960 0.78 +0.7 72,274 - EX31_RS02960 0.78 -0.0 72,324 + +0.7 72,381 + -0.6 72,382 - -0.3 72,400 + +0.1 72,422 - +1.2 72,425 + +1.1 72,425 + +0.5 72,430 + +2.2 72,434 + -0.2 72,477 + +0.9 72,519 - +0.6 72,564 + +1.6 72,569 - +1.8 72,581 + +0.1 72,625 - +0.1 72,641 + +0.1 72,668 + -0.6 72,690 + EX31_RS02965 0.12 -2.3 72,732 - EX31_RS02965 0.18 +0.6 72,745 + EX31_RS02965 0.20 +1.7 72,746 - EX31_RS02965 0.20 +1.3 72,822 + EX31_RS02965 0.31 -0.1 72,825 - EX31_RS02965 0.31 +0.7 72,826 + EX31_RS02965 0.32 +1.4 72,827 - EX31_RS02965 0.32 -0.3 72,854 + EX31_RS02965 0.36 -1.0 72,909 + EX31_RS02965 0.44 +0.3 72,978 + EX31_RS02965 0.54 -1.3 72,978 + EX31_RS02965 0.54 -0.8 72,978 + EX31_RS02965 0.54 +0.4 72,979 - EX31_RS02965 0.54 +2.3 72,979 - EX31_RS02965 0.54 +1.2 72,979 - EX31_RS02965 0.54 +1.0 72,979 - EX31_RS02965 0.54 +0.7 72,979 - EX31_RS02965 0.54 +0.8 72,980 + EX31_RS02965 0.54 -0.3 72,980 + EX31_RS02965 0.54 -1.4 72,980 + EX31_RS02965 0.54 -0.1 72,980 + EX31_RS02965 0.54 -0.9 72,980 + EX31_RS02965 0.54 -1.6 72,982 + EX31_RS02965 0.54 -0.3 72,982 + EX31_RS02965 0.54 -1.5 72,982 + EX31_RS02965 0.54 +0.7 72,983 - EX31_RS02965 0.54 +0.2 72,983 - EX31_RS02965 0.54 +2.3 73,029 - EX31_RS02965 0.61 +1.3 73,046 - EX31_RS02965 0.64 +0.8 73,088 + EX31_RS02965 0.70 +0.1 73,089 - EX31_RS02965 0.70 +1.2 73,089 - EX31_RS02965 0.70 -0.2 73,089 - EX31_RS02965 0.70 -0.1 73,159 - EX31_RS02965 0.80 -0.2 73,178 - EX31_RS02965 0.83 +1.3 73,227 + +0.5 73,228 - +1.0 73,315 - +1.6 73,499 + EX31_RS02970 0.22 -1.5 73,578 + EX31_RS02970 0.35 -1.4 73,579 - EX31_RS02970 0.35 +1.3 73,709 + EX31_RS02970 0.57 -0.4 73,709 + EX31_RS02970 0.57 -0.9 73,710 - EX31_RS02970 0.57 +1.3 73,714 + EX31_RS02970 0.58 -0.7 73,758 - EX31_RS02970 0.65 -0.6 73,758 - EX31_RS02970 0.65 +1.0 73,758 - EX31_RS02970 0.65 -0.3 73,827 + EX31_RS02970 0.77 -0.7 73,884 - EX31_RS02970 0.86 -1.3 73,887 + EX31_RS02970 0.87 +0.1 73,888 - EX31_RS02970 0.87 -1.4 73,908 - +0.6 73,953 - +1.0 73,955 + -0.1 74,086 + -1.5 74,110 - +1.5 74,114 - +0.7 74,114 - +0.7 74,118 + -1.0 74,119 - -0.3 74,119 - -0.6 74,159 + +0.5 74,160 - +3.2 74,160 - +0.3 74,163 - -0.5 74,170 - +0.4 74,170 - +1.0 74,194 + -0.7 74,248 - +1.4 74,248 - +1.9 74,248 - +0.1 74,257 + -0.8 74,257 + -0.8 74,257 + +0.3 74,258 - +0.9 74,258 - +1.1 74,276 + -1.5 74,276 + +0.4 74,276 + +1.7 74,277 - +0.2 74,277 - +1.2 74,277 - -0.6 74,277 - +1.8 74,277 - +0.7 74,277 - +0.1 74,277 - +0.5 74,278 + +0.2 74,278 + +1.1 74,279 - +0.3 74,279 - +1.3 74,279 - +1.6 74,289 + -1.3 74,289 + +0.8 74,290 - -0.6 74,290 - -0.6 74,290 - -0.4
Or see this region's nucleotide sequence