Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02390 and EX31_RS02395 are separated by 134 nucleotides EX31_RS02395 and EX31_RS02400 are separated by 71 nucleotides EX31_RS02400 and EX31_RS02405 are separated by 195 nucleotides
EX31_RS02390: EX31_RS02390 - steroid Delta-isomerase, at 509,364 to 509,699
_RS02390
EX31_RS02395: EX31_RS02395 - hypothetical protein, at 509,834 to 510,193
_RS02395
EX31_RS02400: EX31_RS02400 - MetQ/NlpA family lipoprotein, at 510,265 to 511,077
_RS02400
EX31_RS02405: EX31_RS02405 - MFS transporter, at 511,273 to 512,484
_RS02405
Position (kb) 510
511
512 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 509.361 kb on + strand at 509.361 kb on + strand at 509.361 kb on + strand at 509.474 kb on + strand, within EX31_RS02390 at 509.488 kb on + strand, within EX31_RS02390 at 509.517 kb on + strand, within EX31_RS02390 at 509.519 kb on + strand, within EX31_RS02390 at 509.549 kb on - strand, within EX31_RS02390 at 509.757 kb on + strand at 509.757 kb on + strand at 509.758 kb on - strand at 509.786 kb on + strand at 509.786 kb on + strand at 509.787 kb on - strand at 509.787 kb on - strand at 509.787 kb on - strand at 509.801 kb on - strand at 509.809 kb on + strand at 509.810 kb on - strand at 509.839 kb on + strand at 509.908 kb on + strand, within EX31_RS02395 at 509.951 kb on + strand, within EX31_RS02395 at 509.951 kb on + strand, within EX31_RS02395 at 509.951 kb on + strand, within EX31_RS02395 at 509.952 kb on - strand, within EX31_RS02395 at 509.983 kb on + strand, within EX31_RS02395 at 510.002 kb on + strand, within EX31_RS02395 at 510.002 kb on + strand, within EX31_RS02395 at 510.002 kb on + strand, within EX31_RS02395 at 510.002 kb on + strand, within EX31_RS02395 at 510.003 kb on - strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.004 kb on + strand, within EX31_RS02395 at 510.005 kb on - strand, within EX31_RS02395 at 510.005 kb on - strand, within EX31_RS02395 at 510.005 kb on - strand, within EX31_RS02395 at 510.016 kb on + strand, within EX31_RS02395 at 510.035 kb on + strand, within EX31_RS02395 at 510.035 kb on + strand, within EX31_RS02395 at 510.035 kb on + strand, within EX31_RS02395 at 510.035 kb on + strand, within EX31_RS02395 at 510.045 kb on - strand, within EX31_RS02395 at 510.067 kb on + strand, within EX31_RS02395 at 510.102 kb on + strand, within EX31_RS02395 at 510.121 kb on + strand, within EX31_RS02395 at 510.121 kb on + strand, within EX31_RS02395 at 510.121 kb on + strand, within EX31_RS02395 at 510.121 kb on + strand, within EX31_RS02395 at 510.121 kb on + strand, within EX31_RS02395 at 510.122 kb on - strand, within EX31_RS02395 at 510.136 kb on + strand, within EX31_RS02395 at 510.154 kb on + strand, within EX31_RS02395 at 510.188 kb on - strand at 510.191 kb on + strand at 510.293 kb on + strand at 510.293 kb on + strand at 510.294 kb on - strand at 510.294 kb on - strand at 510.298 kb on - strand at 510.298 kb on - strand at 510.373 kb on - strand, within EX31_RS02400 at 510.393 kb on + strand, within EX31_RS02400 at 510.394 kb on - strand, within EX31_RS02400 at 510.401 kb on + strand, within EX31_RS02400 at 510.402 kb on - strand, within EX31_RS02400 at 510.414 kb on + strand, within EX31_RS02400 at 510.468 kb on - strand, within EX31_RS02400 at 510.468 kb on - strand, within EX31_RS02400 at 510.475 kb on - strand, within EX31_RS02400 at 510.599 kb on - strand, within EX31_RS02400 at 510.719 kb on - strand, within EX31_RS02400 at 510.909 kb on + strand, within EX31_RS02400 at 510.909 kb on + strand, within EX31_RS02400 at 510.911 kb on + strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.981 kb on + strand, within EX31_RS02400 at 510.981 kb on + strand, within EX31_RS02400 at 510.982 kb on - strand, within EX31_RS02400 at 511.043 kb on - strand at 511.167 kb on + strand at 511.186 kb on - strand at 511.186 kb on - strand at 511.215 kb on - strand at 511.265 kb on + strand at 511.266 kb on - strand at 511.291 kb on + strand at 511.292 kb on - strand at 511.621 kb on - strand, within EX31_RS02405 at 511.629 kb on + strand, within EX31_RS02405 at 511.662 kb on + strand, within EX31_RS02405 at 511.662 kb on + strand, within EX31_RS02405 at 511.663 kb on - strand, within EX31_RS02405 at 511.685 kb on - strand, within EX31_RS02405 at 511.688 kb on - strand, within EX31_RS02405 at 511.725 kb on + strand, within EX31_RS02405 at 511.772 kb on + strand, within EX31_RS02405 at 511.773 kb on - strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.778 kb on + strand, within EX31_RS02405 at 511.778 kb on + strand, within EX31_RS02405 at 511.778 kb on + strand, within EX31_RS02405 at 511.804 kb on - strand, within EX31_RS02405 at 511.807 kb on + strand, within EX31_RS02405 at 511.808 kb on - strand, within EX31_RS02405 at 511.948 kb on - strand, within EX31_RS02405 at 511.953 kb on + strand, within EX31_RS02405 at 511.954 kb on - strand, within EX31_RS02405 at 511.954 kb on - strand, within EX31_RS02405 at 511.954 kb on - strand, within EX31_RS02405 at 511.987 kb on - strand, within EX31_RS02405
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 509,361 + -1.5 509,361 + +0.3 509,361 + -1.0 509,474 + EX31_RS02390 0.33 +0.3 509,488 + EX31_RS02390 0.37 -1.1 509,517 + EX31_RS02390 0.46 +0.6 509,519 + EX31_RS02390 0.46 -0.2 509,549 - EX31_RS02390 0.55 +1.1 509,757 + +0.5 509,757 + +0.4 509,758 - -0.5 509,786 + +0.5 509,786 + +1.1 509,787 - +1.0 509,787 - -1.1 509,787 - +3.0 509,801 - +1.9 509,809 + +0.5 509,810 - -0.5 509,839 + -0.8 509,908 + EX31_RS02395 0.21 +0.7 509,951 + EX31_RS02395 0.33 +1.0 509,951 + EX31_RS02395 0.33 +0.7 509,951 + EX31_RS02395 0.33 +1.1 509,952 - EX31_RS02395 0.33 -0.7 509,983 + EX31_RS02395 0.41 +0.2 510,002 + EX31_RS02395 0.47 -1.4 510,002 + EX31_RS02395 0.47 +0.5 510,002 + EX31_RS02395 0.47 +0.5 510,002 + EX31_RS02395 0.47 +0.9 510,003 - EX31_RS02395 0.47 +1.0 510,004 + EX31_RS02395 0.47 +2.5 510,004 + EX31_RS02395 0.47 -0.3 510,004 + EX31_RS02395 0.47 -1.1 510,004 + EX31_RS02395 0.47 -1.2 510,004 + EX31_RS02395 0.47 +0.9 510,004 + EX31_RS02395 0.47 -0.1 510,004 + EX31_RS02395 0.47 +1.9 510,005 - EX31_RS02395 0.47 +0.5 510,005 - EX31_RS02395 0.47 -1.0 510,005 - EX31_RS02395 0.47 +0.0 510,016 + EX31_RS02395 0.51 -0.2 510,035 + EX31_RS02395 0.56 +0.7 510,035 + EX31_RS02395 0.56 -1.1 510,035 + EX31_RS02395 0.56 -0.0 510,035 + EX31_RS02395 0.56 -0.3 510,045 - EX31_RS02395 0.59 +0.3 510,067 + EX31_RS02395 0.65 -0.3 510,102 + EX31_RS02395 0.74 +0.5 510,121 + EX31_RS02395 0.80 +0.5 510,121 + EX31_RS02395 0.80 -0.9 510,121 + EX31_RS02395 0.80 -0.1 510,121 + EX31_RS02395 0.80 -0.7 510,121 + EX31_RS02395 0.80 +0.6 510,122 - EX31_RS02395 0.80 +1.3 510,136 + EX31_RS02395 0.84 +1.0 510,154 + EX31_RS02395 0.89 -1.2 510,188 - -1.5 510,191 + +0.8 510,293 + -0.2 510,293 + -0.9 510,294 - -1.0 510,294 - +0.4 510,298 - -1.3 510,298 - -0.4 510,373 - EX31_RS02400 0.13 -0.1 510,393 + EX31_RS02400 0.16 +1.7 510,394 - EX31_RS02400 0.16 +0.7 510,401 + EX31_RS02400 0.17 +0.4 510,402 - EX31_RS02400 0.17 -0.9 510,414 + EX31_RS02400 0.18 +0.0 510,468 - EX31_RS02400 0.25 -0.8 510,468 - EX31_RS02400 0.25 +0.5 510,475 - EX31_RS02400 0.26 +2.0 510,599 - EX31_RS02400 0.41 +0.0 510,719 - EX31_RS02400 0.56 +0.2 510,909 + EX31_RS02400 0.79 +0.8 510,909 + EX31_RS02400 0.79 +0.5 510,911 + EX31_RS02400 0.79 -0.7 510,912 - EX31_RS02400 0.80 +0.0 510,912 - EX31_RS02400 0.80 -0.5 510,912 - EX31_RS02400 0.80 +0.0 510,912 - EX31_RS02400 0.80 -1.9 510,912 - EX31_RS02400 0.80 -1.7 510,912 - EX31_RS02400 0.80 +0.3 510,981 + EX31_RS02400 0.88 -2.5 510,981 + EX31_RS02400 0.88 +1.3 510,982 - EX31_RS02400 0.88 +0.5 511,043 - +4.0 511,167 + -0.4 511,186 - -2.2 511,186 - +0.6 511,215 - +0.8 511,265 + -1.6 511,266 - -1.3 511,291 + -0.4 511,292 - -1.1 511,621 - EX31_RS02405 0.29 +1.0 511,629 + EX31_RS02405 0.29 -0.4 511,662 + EX31_RS02405 0.32 +0.1 511,662 + EX31_RS02405 0.32 -0.9 511,663 - EX31_RS02405 0.32 -0.3 511,685 - EX31_RS02405 0.34 -0.3 511,688 - EX31_RS02405 0.34 -1.9 511,725 + EX31_RS02405 0.37 +0.0 511,772 + EX31_RS02405 0.41 +0.2 511,773 - EX31_RS02405 0.41 -0.4 511,776 + EX31_RS02405 0.42 +0.9 511,776 + EX31_RS02405 0.42 -0.8 511,776 + EX31_RS02405 0.42 +0.7 511,776 + EX31_RS02405 0.42 +1.0 511,776 + EX31_RS02405 0.42 -0.4 511,776 + EX31_RS02405 0.42 -0.7 511,776 + EX31_RS02405 0.42 -0.5 511,776 + EX31_RS02405 0.42 +2.0 511,776 + EX31_RS02405 0.42 +1.2 511,776 + EX31_RS02405 0.42 -0.7 511,776 + EX31_RS02405 0.42 -0.4 511,776 + EX31_RS02405 0.42 -2.1 511,776 + EX31_RS02405 0.42 +0.2 511,776 + EX31_RS02405 0.42 +0.0 511,776 + EX31_RS02405 0.42 -1.3 511,777 - EX31_RS02405 0.42 +0.0 511,777 - EX31_RS02405 0.42 -0.7 511,777 - EX31_RS02405 0.42 -0.8 511,777 - EX31_RS02405 0.42 -1.3 511,777 - EX31_RS02405 0.42 +0.0 511,777 - EX31_RS02405 0.42 -0.1 511,777 - EX31_RS02405 0.42 -2.5 511,777 - EX31_RS02405 0.42 -0.3 511,777 - EX31_RS02405 0.42 +1.4 511,778 + EX31_RS02405 0.42 +1.2 511,778 + EX31_RS02405 0.42 -1.1 511,778 + EX31_RS02405 0.42 -0.1 511,804 - EX31_RS02405 0.44 +1.0 511,807 + EX31_RS02405 0.44 -1.1 511,808 - EX31_RS02405 0.44 +0.2 511,948 - EX31_RS02405 0.56 -0.5 511,953 + EX31_RS02405 0.56 -0.4 511,954 - EX31_RS02405 0.56 +0.7 511,954 - EX31_RS02405 0.56 -0.3 511,954 - EX31_RS02405 0.56 +1.1 511,987 - EX31_RS02405 0.59 -1.7
Or see this region's nucleotide sequence