Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25445 and CA265_RS25450 are separated by 163 nucleotides CA265_RS25450 and CA265_RS25455 are separated by 92 nucleotides CA265_RS25455 and CA265_RS25460 are separated by 162 nucleotides
CA265_RS25445: CA265_RS25445 - ABC transporter, at 6,177,434 to 6,178,663
_RS25445
CA265_RS25450: CA265_RS25450 - iron-sulfur cluster assembly accessory protein, at 6,178,827 to 6,179,180
_RS25450
CA265_RS25455: CA265_RS25455 - hypothetical protein, at 6,179,273 to 6,179,803
_RS25455
CA265_RS25460: CA265_RS25460 - methylmalonyl-CoA mutase, at 6,179,966 to 6,181,513
_RS25460
Position (kb)
6178
6179
6180 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6177.896 kb on + strand, within CA265_RS25445 at 6177.962 kb on - strand, within CA265_RS25445 at 6177.985 kb on - strand, within CA265_RS25445 at 6178.036 kb on - strand, within CA265_RS25445 at 6178.085 kb on + strand, within CA265_RS25445 at 6178.085 kb on + strand, within CA265_RS25445 at 6178.086 kb on - strand, within CA265_RS25445 at 6178.116 kb on - strand, within CA265_RS25445 at 6178.220 kb on - strand, within CA265_RS25445 at 6178.274 kb on + strand, within CA265_RS25445 at 6178.318 kb on - strand, within CA265_RS25445 at 6178.387 kb on + strand, within CA265_RS25445 at 6178.439 kb on - strand, within CA265_RS25445 at 6178.453 kb on + strand, within CA265_RS25445 at 6178.480 kb on + strand, within CA265_RS25445 at 6178.529 kb on + strand, within CA265_RS25445 at 6178.583 kb on - strand at 6178.600 kb on + strand at 6178.600 kb on + strand at 6178.601 kb on - strand at 6178.604 kb on - strand at 6178.624 kb on + strand at 6178.657 kb on - strand at 6178.675 kb on - strand at 6178.694 kb on - strand at 6178.707 kb on + strand at 6178.726 kb on + strand at 6178.726 kb on + strand at 6178.728 kb on + strand at 6178.729 kb on - strand at 6178.729 kb on - strand at 6178.734 kb on - strand at 6178.735 kb on + strand at 6178.805 kb on - strand at 6178.806 kb on + strand at 6178.806 kb on + strand at 6178.807 kb on - strand at 6178.807 kb on - strand at 6178.812 kb on - strand at 6178.812 kb on - strand at 6178.817 kb on - strand at 6178.820 kb on - strand at 6178.824 kb on + strand at 6178.825 kb on - strand at 6179.065 kb on - strand, within CA265_RS25450 at 6179.111 kb on + strand, within CA265_RS25450 at 6179.111 kb on + strand, within CA265_RS25450 at 6179.213 kb on + strand at 6179.272 kb on + strand at 6179.292 kb on + strand at 6179.299 kb on + strand at 6179.299 kb on + strand at 6179.301 kb on + strand at 6179.301 kb on + strand at 6179.355 kb on + strand, within CA265_RS25455 at 6179.356 kb on - strand, within CA265_RS25455 at 6179.385 kb on + strand, within CA265_RS25455 at 6179.385 kb on + strand, within CA265_RS25455 at 6179.422 kb on + strand, within CA265_RS25455 at 6179.433 kb on + strand, within CA265_RS25455 at 6179.434 kb on - strand, within CA265_RS25455 at 6179.464 kb on - strand, within CA265_RS25455 at 6179.489 kb on - strand, within CA265_RS25455 at 6179.489 kb on - strand, within CA265_RS25455 at 6179.535 kb on + strand, within CA265_RS25455 at 6179.545 kb on + strand, within CA265_RS25455 at 6179.582 kb on - strand, within CA265_RS25455 at 6179.601 kb on + strand, within CA265_RS25455 at 6179.645 kb on - strand, within CA265_RS25455 at 6179.660 kb on - strand, within CA265_RS25455 at 6179.670 kb on + strand, within CA265_RS25455 at 6179.773 kb on + strand at 6179.791 kb on + strand at 6179.792 kb on - strand at 6179.792 kb on - strand at 6179.891 kb on + strand at 6179.941 kb on + strand at 6179.942 kb on - strand at 6179.958 kb on - strand at 6179.958 kb on - strand at 6179.962 kb on + strand at 6180.085 kb on + strand at 6180.086 kb on - strand at 6180.089 kb on + strand at 6180.090 kb on - strand at 6180.119 kb on + strand at 6180.119 kb on + strand at 6180.165 kb on - strand, within CA265_RS25460 at 6180.165 kb on - strand, within CA265_RS25460 at 6180.169 kb on - strand, within CA265_RS25460
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 6,177,896 + CA265_RS25445 0.38 +0.4 6,177,962 - CA265_RS25445 0.43 +0.5 6,177,985 - CA265_RS25445 0.45 -0.4 6,178,036 - CA265_RS25445 0.49 -1.2 6,178,085 + CA265_RS25445 0.53 +0.9 6,178,085 + CA265_RS25445 0.53 -0.1 6,178,086 - CA265_RS25445 0.53 -0.3 6,178,116 - CA265_RS25445 0.55 -0.5 6,178,220 - CA265_RS25445 0.64 +0.3 6,178,274 + CA265_RS25445 0.68 -0.5 6,178,318 - CA265_RS25445 0.72 -0.0 6,178,387 + CA265_RS25445 0.77 +0.7 6,178,439 - CA265_RS25445 0.82 -0.6 6,178,453 + CA265_RS25445 0.83 +1.3 6,178,480 + CA265_RS25445 0.85 +0.0 6,178,529 + CA265_RS25445 0.89 +0.1 6,178,583 - +0.2 6,178,600 + +0.1 6,178,600 + -0.0 6,178,601 - -0.2 6,178,604 - +1.5 6,178,624 + -0.1 6,178,657 - -0.5 6,178,675 - +1.0 6,178,694 - +1.3 6,178,707 + +0.2 6,178,726 + -0.5 6,178,726 + +0.9 6,178,728 + +0.7 6,178,729 - +0.3 6,178,729 - +1.6 6,178,734 - +0.5 6,178,735 + -0.2 6,178,805 - +1.1 6,178,806 + -1.3 6,178,806 + +0.1 6,178,807 - +0.7 6,178,807 - -0.2 6,178,812 - +0.0 6,178,812 - -0.9 6,178,817 - +0.7 6,178,820 - +0.1 6,178,824 + +0.5 6,178,825 - +0.1 6,179,065 - CA265_RS25450 0.67 -1.6 6,179,111 + CA265_RS25450 0.80 -2.0 6,179,111 + CA265_RS25450 0.80 -1.7 6,179,213 + -0.5 6,179,272 + +0.8 6,179,292 + -0.2 6,179,299 + +0.1 6,179,299 + -1.2 6,179,301 + +0.5 6,179,301 + -0.6 6,179,355 + CA265_RS25455 0.15 -0.1 6,179,356 - CA265_RS25455 0.16 -0.3 6,179,385 + CA265_RS25455 0.21 +0.6 6,179,385 + CA265_RS25455 0.21 -0.2 6,179,422 + CA265_RS25455 0.28 -0.2 6,179,433 + CA265_RS25455 0.30 -0.9 6,179,434 - CA265_RS25455 0.30 -0.2 6,179,464 - CA265_RS25455 0.36 -0.4 6,179,489 - CA265_RS25455 0.41 -0.0 6,179,489 - CA265_RS25455 0.41 +2.0 6,179,535 + CA265_RS25455 0.49 +0.6 6,179,545 + CA265_RS25455 0.51 +0.1 6,179,582 - CA265_RS25455 0.58 +2.2 6,179,601 + CA265_RS25455 0.62 +0.3 6,179,645 - CA265_RS25455 0.70 +0.5 6,179,660 - CA265_RS25455 0.73 +0.1 6,179,670 + CA265_RS25455 0.75 -0.8 6,179,773 + +0.8 6,179,791 + -1.8 6,179,792 - -1.3 6,179,792 - -0.9 6,179,891 + +1.1 6,179,941 + +0.2 6,179,942 - +1.2 6,179,958 - -0.8 6,179,958 - +0.7 6,179,962 + +0.9 6,180,085 + -0.3 6,180,086 - -0.7 6,180,089 + -1.0 6,180,090 - -0.4 6,180,119 + +1.4 6,180,119 + +0.9 6,180,165 - CA265_RS25460 0.13 -0.4 6,180,165 - CA265_RS25460 0.13 +0.4 6,180,169 - CA265_RS25460 0.13 +0.1
Or see this region's nucleotide sequence