Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18310 and CA265_RS18315 are separated by 405 nucleotides CA265_RS18315 and CA265_RS18320 are separated by 154 nucleotides
CA265_RS18310: CA265_RS18310 - thioredoxin family protein, at 4,561,571 to 4,562,170
_RS18310
CA265_RS18315: CA265_RS18315 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase, at 4,562,576 to 4,563,361
_RS18315
CA265_RS18320: CA265_RS18320 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 4,563,516 to 4,564,595
_RS18320
Position (kb)
4562
4563
4564 Strain fitness (log2 ratio)
-2
-1
0
1 at 4561.679 kb on - strand, within CA265_RS18310 at 4561.767 kb on - strand, within CA265_RS18310 at 4561.821 kb on - strand, within CA265_RS18310 at 4561.841 kb on - strand, within CA265_RS18310 at 4561.891 kb on + strand, within CA265_RS18310 at 4561.954 kb on - strand, within CA265_RS18310 at 4562.050 kb on - strand, within CA265_RS18310 at 4562.061 kb on + strand, within CA265_RS18310 at 4562.066 kb on + strand, within CA265_RS18310 at 4562.127 kb on + strand at 4562.128 kb on - strand at 4562.128 kb on - strand at 4562.128 kb on - strand at 4562.133 kb on - strand at 4562.253 kb on - strand at 4562.253 kb on - strand at 4562.316 kb on + strand at 4562.317 kb on - strand at 4562.352 kb on + strand at 4562.460 kb on - strand at 4562.462 kb on - strand at 4562.597 kb on + strand at 4562.597 kb on + strand at 4562.633 kb on + strand at 4562.659 kb on - strand, within CA265_RS18315 at 4562.659 kb on - strand, within CA265_RS18315 at 4562.662 kb on + strand, within CA265_RS18315 at 4562.662 kb on + strand, within CA265_RS18315 at 4562.663 kb on - strand, within CA265_RS18315 at 4562.663 kb on - strand, within CA265_RS18315 at 4562.840 kb on - strand, within CA265_RS18315 at 4562.840 kb on - strand, within CA265_RS18315 at 4562.840 kb on - strand, within CA265_RS18315 at 4562.881 kb on - strand, within CA265_RS18315 at 4562.883 kb on + strand, within CA265_RS18315 at 4562.884 kb on - strand, within CA265_RS18315 at 4562.982 kb on - strand, within CA265_RS18315 at 4562.998 kb on + strand, within CA265_RS18315 at 4563.036 kb on + strand, within CA265_RS18315 at 4563.192 kb on - strand, within CA265_RS18315 at 4563.222 kb on - strand, within CA265_RS18315 at 4563.242 kb on + strand, within CA265_RS18315 at 4563.261 kb on + strand, within CA265_RS18315 at 4563.261 kb on + strand, within CA265_RS18315 at 4563.266 kb on - strand, within CA265_RS18315 at 4563.333 kb on - strand at 4563.417 kb on - strand at 4563.419 kb on + strand at 4563.443 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 4,561,679 - CA265_RS18310 0.18 -0.9 4,561,767 - CA265_RS18310 0.33 +0.9 4,561,821 - CA265_RS18310 0.42 +0.1 4,561,841 - CA265_RS18310 0.45 -1.1 4,561,891 + CA265_RS18310 0.53 -0.6 4,561,954 - CA265_RS18310 0.64 +0.4 4,562,050 - CA265_RS18310 0.80 -0.0 4,562,061 + CA265_RS18310 0.82 +1.3 4,562,066 + CA265_RS18310 0.82 -0.6 4,562,127 + -0.2 4,562,128 - +0.8 4,562,128 - +0.1 4,562,128 - -0.6 4,562,133 - +0.4 4,562,253 - -0.2 4,562,253 - -0.2 4,562,316 + +1.0 4,562,317 - +0.6 4,562,352 + +1.1 4,562,460 - -0.5 4,562,462 - +0.7 4,562,597 + -0.4 4,562,597 + -0.8 4,562,633 + +0.7 4,562,659 - CA265_RS18315 0.11 -1.4 4,562,659 - CA265_RS18315 0.11 -0.8 4,562,662 + CA265_RS18315 0.11 +0.1 4,562,662 + CA265_RS18315 0.11 -0.4 4,562,663 - CA265_RS18315 0.11 -0.2 4,562,663 - CA265_RS18315 0.11 -1.0 4,562,840 - CA265_RS18315 0.34 +0.6 4,562,840 - CA265_RS18315 0.34 -1.2 4,562,840 - CA265_RS18315 0.34 +0.1 4,562,881 - CA265_RS18315 0.39 -0.5 4,562,883 + CA265_RS18315 0.39 -1.6 4,562,884 - CA265_RS18315 0.39 +0.5 4,562,982 - CA265_RS18315 0.52 -0.2 4,562,998 + CA265_RS18315 0.54 -1.7 4,563,036 + CA265_RS18315 0.59 -1.0 4,563,192 - CA265_RS18315 0.78 +0.0 4,563,222 - CA265_RS18315 0.82 -1.3 4,563,242 + CA265_RS18315 0.85 -1.3 4,563,261 + CA265_RS18315 0.87 -1.9 4,563,261 + CA265_RS18315 0.87 -0.4 4,563,266 - CA265_RS18315 0.88 +0.2 4,563,333 - -0.6 4,563,417 - -0.0 4,563,419 + +0.6 4,563,443 + +1.1
Or see this region's nucleotide sequence