Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS17860 and CA265_RS17865 are separated by 14 nucleotides CA265_RS17865 and CA265_RS17870 are separated by 96 nucleotides CA265_RS17870 and CA265_RS17875 are separated by 123 nucleotides
CA265_RS17860: CA265_RS17860 - hypothetical protein, at 4,468,026 to 4,468,304
_RS17860
CA265_RS17865: CA265_RS17865 - hypothetical protein, at 4,468,319 to 4,469,056
_RS17865
CA265_RS17870: CA265_RS17870 - polymerase, at 4,469,153 to 4,470,283
_RS17870
CA265_RS17875: CA265_RS17875 - hypothetical protein, at 4,470,407 to 4,472,560
_RS17875
Position (kb)
4469
4470
4471 Strain fitness (log2 ratio)
-2
-1
0
1 at 4468.173 kb on - strand, within CA265_RS17860 at 4468.202 kb on + strand, within CA265_RS17860 at 4468.203 kb on - strand, within CA265_RS17860 at 4468.203 kb on - strand, within CA265_RS17860 at 4468.214 kb on - strand, within CA265_RS17860 at 4468.254 kb on + strand, within CA265_RS17860 at 4468.254 kb on + strand, within CA265_RS17860 at 4468.265 kb on + strand, within CA265_RS17860 at 4468.293 kb on - strand at 4468.335 kb on - strand at 4468.376 kb on + strand at 4468.380 kb on + strand at 4468.381 kb on - strand at 4468.387 kb on + strand at 4468.514 kb on - strand, within CA265_RS17865 at 4468.552 kb on + strand, within CA265_RS17865 at 4468.553 kb on - strand, within CA265_RS17865 at 4468.630 kb on + strand, within CA265_RS17865 at 4468.631 kb on - strand, within CA265_RS17865 at 4468.638 kb on - strand, within CA265_RS17865 at 4468.685 kb on - strand, within CA265_RS17865 at 4468.688 kb on + strand, within CA265_RS17865 at 4468.698 kb on + strand, within CA265_RS17865 at 4468.730 kb on + strand, within CA265_RS17865 at 4468.731 kb on - strand, within CA265_RS17865 at 4468.731 kb on - strand, within CA265_RS17865 at 4468.740 kb on - strand, within CA265_RS17865 at 4468.745 kb on + strand, within CA265_RS17865 at 4468.801 kb on + strand, within CA265_RS17865 at 4468.802 kb on - strand, within CA265_RS17865 at 4468.842 kb on - strand, within CA265_RS17865 at 4468.852 kb on + strand, within CA265_RS17865 at 4468.907 kb on - strand, within CA265_RS17865 at 4468.960 kb on + strand, within CA265_RS17865 at 4468.970 kb on + strand, within CA265_RS17865 at 4468.990 kb on + strand at 4469.037 kb on - strand at 4469.050 kb on + strand at 4469.051 kb on - strand at 4469.053 kb on - strand at 4469.053 kb on - strand at 4469.061 kb on - strand at 4469.126 kb on + strand at 4469.165 kb on + strand at 4469.166 kb on - strand at 4469.192 kb on - strand at 4469.274 kb on + strand, within CA265_RS17870 at 4469.295 kb on - strand, within CA265_RS17870 at 4469.345 kb on + strand, within CA265_RS17870 at 4469.435 kb on - strand, within CA265_RS17870 at 4469.461 kb on + strand, within CA265_RS17870 at 4469.461 kb on + strand, within CA265_RS17870 at 4469.462 kb on - strand, within CA265_RS17870 at 4469.492 kb on - strand, within CA265_RS17870 at 4469.551 kb on + strand, within CA265_RS17870 at 4469.615 kb on + strand, within CA265_RS17870 at 4469.615 kb on + strand, within CA265_RS17870 at 4469.616 kb on - strand, within CA265_RS17870 at 4469.618 kb on - strand, within CA265_RS17870 at 4469.618 kb on - strand, within CA265_RS17870 at 4469.625 kb on + strand, within CA265_RS17870 at 4469.646 kb on - strand, within CA265_RS17870 at 4469.655 kb on + strand, within CA265_RS17870 at 4469.655 kb on + strand, within CA265_RS17870 at 4469.655 kb on + strand, within CA265_RS17870 at 4469.659 kb on + strand, within CA265_RS17870 at 4469.678 kb on + strand, within CA265_RS17870 at 4469.747 kb on + strand, within CA265_RS17870 at 4469.759 kb on - strand, within CA265_RS17870 at 4469.810 kb on + strand, within CA265_RS17870 at 4469.810 kb on + strand, within CA265_RS17870 at 4469.938 kb on + strand, within CA265_RS17870 at 4469.944 kb on + strand, within CA265_RS17870 at 4469.944 kb on + strand, within CA265_RS17870 at 4470.082 kb on - strand, within CA265_RS17870 at 4470.106 kb on - strand, within CA265_RS17870 at 4470.193 kb on - strand at 4470.205 kb on - strand at 4470.258 kb on - strand at 4470.267 kb on + strand at 4470.291 kb on - strand at 4470.291 kb on - strand at 4470.291 kb on - strand at 4470.297 kb on + strand at 4470.298 kb on - strand at 4470.321 kb on + strand at 4470.323 kb on + strand at 4470.323 kb on + strand at 4470.325 kb on + strand at 4470.419 kb on - strand at 4470.437 kb on + strand at 4470.448 kb on + strand at 4470.448 kb on + strand at 4470.610 kb on + strand at 4470.687 kb on + strand, within CA265_RS17875 at 4470.687 kb on + strand, within CA265_RS17875 at 4470.687 kb on + strand, within CA265_RS17875 at 4470.687 kb on + strand, within CA265_RS17875 at 4470.687 kb on + strand, within CA265_RS17875 at 4470.691 kb on + strand, within CA265_RS17875 at 4470.696 kb on - strand, within CA265_RS17875 at 4470.746 kb on - strand, within CA265_RS17875 at 4470.798 kb on - strand, within CA265_RS17875 at 4470.798 kb on - strand, within CA265_RS17875 at 4470.819 kb on - strand, within CA265_RS17875 at 4470.821 kb on + strand, within CA265_RS17875 at 4470.865 kb on + strand, within CA265_RS17875 at 4470.907 kb on + strand, within CA265_RS17875 at 4470.969 kb on + strand, within CA265_RS17875 at 4471.098 kb on + strand, within CA265_RS17875 at 4471.098 kb on + strand, within CA265_RS17875 at 4471.099 kb on - strand, within CA265_RS17875 at 4471.142 kb on + strand, within CA265_RS17875 at 4471.142 kb on + strand, within CA265_RS17875 at 4471.143 kb on - strand, within CA265_RS17875 at 4471.160 kb on - strand at 4471.204 kb on + strand, within CA265_RS17875 at 4471.208 kb on - strand, within CA265_RS17875 at 4471.224 kb on - strand, within CA265_RS17875 at 4471.246 kb on + strand, within CA265_RS17875
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 4,468,173 - CA265_RS17860 0.53 +0.6 4,468,202 + CA265_RS17860 0.63 +0.2 4,468,203 - CA265_RS17860 0.63 +0.6 4,468,203 - CA265_RS17860 0.63 +0.0 4,468,214 - CA265_RS17860 0.67 +0.9 4,468,254 + CA265_RS17860 0.82 +0.3 4,468,254 + CA265_RS17860 0.82 +0.7 4,468,265 + CA265_RS17860 0.86 +0.9 4,468,293 - -0.9 4,468,335 - +0.4 4,468,376 + -0.2 4,468,380 + +0.4 4,468,381 - +0.5 4,468,387 + +0.5 4,468,514 - CA265_RS17865 0.26 +0.1 4,468,552 + CA265_RS17865 0.32 +0.1 4,468,553 - CA265_RS17865 0.32 -0.0 4,468,630 + CA265_RS17865 0.42 -0.2 4,468,631 - CA265_RS17865 0.42 +0.4 4,468,638 - CA265_RS17865 0.43 +0.6 4,468,685 - CA265_RS17865 0.50 -0.0 4,468,688 + CA265_RS17865 0.50 +0.2 4,468,698 + CA265_RS17865 0.51 +0.9 4,468,730 + CA265_RS17865 0.56 -0.0 4,468,731 - CA265_RS17865 0.56 -0.1 4,468,731 - CA265_RS17865 0.56 -0.0 4,468,740 - CA265_RS17865 0.57 +0.7 4,468,745 + CA265_RS17865 0.58 -0.3 4,468,801 + CA265_RS17865 0.65 -0.4 4,468,802 - CA265_RS17865 0.65 +0.6 4,468,842 - CA265_RS17865 0.71 -0.6 4,468,852 + CA265_RS17865 0.72 -0.1 4,468,907 - CA265_RS17865 0.80 +1.1 4,468,960 + CA265_RS17865 0.87 +0.2 4,468,970 + CA265_RS17865 0.88 -0.1 4,468,990 + +1.4 4,469,037 - +1.2 4,469,050 + +0.5 4,469,051 - +0.2 4,469,053 - +0.5 4,469,053 - +0.5 4,469,061 - +0.3 4,469,126 + +0.3 4,469,165 + +0.1 4,469,166 - -0.9 4,469,192 - +0.3 4,469,274 + CA265_RS17870 0.11 -1.2 4,469,295 - CA265_RS17870 0.13 -1.4 4,469,345 + CA265_RS17870 0.17 +0.2 4,469,435 - CA265_RS17870 0.25 -0.7 4,469,461 + CA265_RS17870 0.27 -0.3 4,469,461 + CA265_RS17870 0.27 +0.1 4,469,462 - CA265_RS17870 0.27 -1.6 4,469,492 - CA265_RS17870 0.30 -1.5 4,469,551 + CA265_RS17870 0.35 -0.7 4,469,615 + CA265_RS17870 0.41 +0.2 4,469,615 + CA265_RS17870 0.41 -1.0 4,469,616 - CA265_RS17870 0.41 -0.2 4,469,618 - CA265_RS17870 0.41 -0.4 4,469,618 - CA265_RS17870 0.41 -0.4 4,469,625 + CA265_RS17870 0.42 -0.9 4,469,646 - CA265_RS17870 0.44 +0.8 4,469,655 + CA265_RS17870 0.44 -0.5 4,469,655 + CA265_RS17870 0.44 -0.2 4,469,655 + CA265_RS17870 0.44 -1.4 4,469,659 + CA265_RS17870 0.45 -0.9 4,469,678 + CA265_RS17870 0.46 +0.9 4,469,747 + CA265_RS17870 0.53 +0.2 4,469,759 - CA265_RS17870 0.54 -1.5 4,469,810 + CA265_RS17870 0.58 +1.6 4,469,810 + CA265_RS17870 0.58 -0.9 4,469,938 + CA265_RS17870 0.69 -0.4 4,469,944 + CA265_RS17870 0.70 -0.6 4,469,944 + CA265_RS17870 0.70 -0.2 4,470,082 - CA265_RS17870 0.82 +0.1 4,470,106 - CA265_RS17870 0.84 -1.2 4,470,193 - -0.7 4,470,205 - -0.0 4,470,258 - -2.0 4,470,267 + -0.7 4,470,291 - -0.5 4,470,291 - +0.5 4,470,291 - +1.1 4,470,297 + +0.2 4,470,298 - +0.4 4,470,321 + +0.2 4,470,323 + +0.5 4,470,323 + +0.4 4,470,325 + -0.6 4,470,419 - +0.9 4,470,437 + -0.1 4,470,448 + +0.0 4,470,448 + +1.5 4,470,610 + +0.6 4,470,687 + CA265_RS17875 0.13 -0.0 4,470,687 + CA265_RS17875 0.13 +0.2 4,470,687 + CA265_RS17875 0.13 +0.7 4,470,687 + CA265_RS17875 0.13 +0.2 4,470,687 + CA265_RS17875 0.13 +0.6 4,470,691 + CA265_RS17875 0.13 +0.0 4,470,696 - CA265_RS17875 0.13 +0.1 4,470,746 - CA265_RS17875 0.16 +0.3 4,470,798 - CA265_RS17875 0.18 -1.2 4,470,798 - CA265_RS17875 0.18 +1.0 4,470,819 - CA265_RS17875 0.19 +0.1 4,470,821 + CA265_RS17875 0.19 +0.4 4,470,865 + CA265_RS17875 0.21 +0.4 4,470,907 + CA265_RS17875 0.23 +0.1 4,470,969 + CA265_RS17875 0.26 +0.1 4,471,098 + CA265_RS17875 0.32 +0.4 4,471,098 + CA265_RS17875 0.32 -0.4 4,471,099 - CA265_RS17875 0.32 -1.0 4,471,142 + CA265_RS17875 0.34 +0.7 4,471,142 + CA265_RS17875 0.34 +0.4 4,471,143 - CA265_RS17875 0.34 -1.2 4,471,160 - +0.6 4,471,204 + CA265_RS17875 0.37 +0.4 4,471,208 - CA265_RS17875 0.37 +0.1 4,471,224 - CA265_RS17875 0.38 +0.6 4,471,246 + CA265_RS17875 0.39 +1.0
Or see this region's nucleotide sequence