Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS17030 and CA265_RS17035 are separated by 186 nucleotides CA265_RS17035 and CA265_RS17040 overlap by 17 nucleotides CA265_RS17040 and CA265_RS17045 are separated by 4 nucleotides
CA265_RS17030: CA265_RS17030 - band 7 protein, at 4,245,730 to 4,246,635
_RS17030
CA265_RS17035: CA265_RS17035 - hypothetical protein, at 4,246,822 to 4,247,394
_RS17035
CA265_RS17040: CA265_RS17040 - hypothetical protein, at 4,247,378 to 4,247,836
_RS17040
CA265_RS17045: CA265_RS17045 - hypothetical protein, at 4,247,841 to 4,249,241
_RS17045
Position (kb)
4246
4247
4248 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4245.873 kb on + strand, within CA265_RS17030 at 4245.939 kb on - strand, within CA265_RS17030 at 4245.960 kb on + strand, within CA265_RS17030 at 4245.961 kb on - strand, within CA265_RS17030 at 4246.006 kb on + strand, within CA265_RS17030 at 4246.006 kb on + strand, within CA265_RS17030 at 4246.323 kb on - strand, within CA265_RS17030 at 4246.342 kb on - strand, within CA265_RS17030 at 4246.347 kb on + strand, within CA265_RS17030 at 4246.388 kb on + strand, within CA265_RS17030 at 4246.388 kb on + strand, within CA265_RS17030 at 4246.388 kb on + strand, within CA265_RS17030 at 4246.389 kb on - strand, within CA265_RS17030 at 4246.464 kb on - strand, within CA265_RS17030 at 4246.464 kb on - strand, within CA265_RS17030 at 4246.464 kb on - strand, within CA265_RS17030 at 4246.588 kb on - strand at 4246.622 kb on + strand at 4246.665 kb on + strand at 4246.686 kb on + strand at 4246.703 kb on - strand at 4246.797 kb on + strand at 4246.807 kb on + strand at 4246.864 kb on + strand at 4246.865 kb on - strand at 4246.865 kb on - strand at 4246.968 kb on + strand, within CA265_RS17035 at 4246.969 kb on - strand, within CA265_RS17035 at 4247.022 kb on + strand, within CA265_RS17035 at 4247.155 kb on + strand, within CA265_RS17035 at 4247.209 kb on - strand, within CA265_RS17035 at 4247.255 kb on + strand, within CA265_RS17035 at 4247.265 kb on + strand, within CA265_RS17035 at 4247.279 kb on + strand, within CA265_RS17035 at 4247.279 kb on + strand, within CA265_RS17035 at 4247.282 kb on - strand, within CA265_RS17035 at 4247.283 kb on + strand, within CA265_RS17035 at 4247.287 kb on + strand, within CA265_RS17035 at 4247.287 kb on + strand, within CA265_RS17035 at 4247.293 kb on + strand, within CA265_RS17035 at 4247.295 kb on + strand, within CA265_RS17035 at 4247.303 kb on + strand, within CA265_RS17035 at 4247.303 kb on + strand, within CA265_RS17035 at 4247.325 kb on - strand, within CA265_RS17035 at 4247.367 kb on + strand at 4247.380 kb on - strand at 4247.404 kb on + strand at 4247.404 kb on + strand at 4247.404 kb on + strand at 4247.405 kb on - strand at 4247.576 kb on + strand, within CA265_RS17040 at 4247.598 kb on - strand, within CA265_RS17040 at 4247.739 kb on + strand, within CA265_RS17040 at 4247.754 kb on - strand, within CA265_RS17040 at 4247.776 kb on + strand, within CA265_RS17040 at 4247.802 kb on - strand at 4247.830 kb on + strand at 4247.836 kb on + strand at 4247.842 kb on + strand at 4247.850 kb on + strand at 4247.862 kb on + strand at 4247.891 kb on + strand at 4247.894 kb on - strand at 4247.928 kb on + strand at 4247.928 kb on + strand at 4247.928 kb on + strand at 4247.982 kb on + strand, within CA265_RS17045 at 4247.983 kb on - strand, within CA265_RS17045 at 4247.983 kb on - strand, within CA265_RS17045 at 4247.984 kb on + strand, within CA265_RS17045 at 4247.984 kb on + strand, within CA265_RS17045 at 4247.984 kb on + strand, within CA265_RS17045 at 4247.985 kb on - strand, within CA265_RS17045 at 4247.993 kb on + strand, within CA265_RS17045 at 4248.004 kb on + strand, within CA265_RS17045 at 4248.004 kb on + strand, within CA265_RS17045 at 4248.004 kb on + strand, within CA265_RS17045 at 4248.004 kb on + strand, within CA265_RS17045 at 4248.027 kb on - strand, within CA265_RS17045 at 4248.028 kb on + strand, within CA265_RS17045 at 4248.060 kb on + strand, within CA265_RS17045 at 4248.061 kb on - strand, within CA265_RS17045 at 4248.161 kb on + strand, within CA265_RS17045 at 4248.162 kb on - strand, within CA265_RS17045 at 4248.194 kb on + strand, within CA265_RS17045 at 4248.209 kb on + strand, within CA265_RS17045 at 4248.241 kb on - strand, within CA265_RS17045 at 4248.282 kb on - strand, within CA265_RS17045 at 4248.302 kb on - strand, within CA265_RS17045 at 4248.319 kb on + strand, within CA265_RS17045 at 4248.319 kb on + strand, within CA265_RS17045 at 4248.320 kb on - strand, within CA265_RS17045 at 4248.325 kb on - strand, within CA265_RS17045 at 4248.329 kb on + strand, within CA265_RS17045 at 4248.359 kb on + strand, within CA265_RS17045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 4,245,873 + CA265_RS17030 0.16 -1.4 4,245,939 - CA265_RS17030 0.23 +0.3 4,245,960 + CA265_RS17030 0.25 -1.4 4,245,961 - CA265_RS17030 0.25 +0.3 4,246,006 + CA265_RS17030 0.30 -2.6 4,246,006 + CA265_RS17030 0.30 -1.4 4,246,323 - CA265_RS17030 0.65 +0.4 4,246,342 - CA265_RS17030 0.68 +0.3 4,246,347 + CA265_RS17030 0.68 -1.2 4,246,388 + CA265_RS17030 0.73 -1.1 4,246,388 + CA265_RS17030 0.73 -1.5 4,246,388 + CA265_RS17030 0.73 -2.8 4,246,389 - CA265_RS17030 0.73 +0.9 4,246,464 - CA265_RS17030 0.81 +0.7 4,246,464 - CA265_RS17030 0.81 +0.7 4,246,464 - CA265_RS17030 0.81 +1.8 4,246,588 - -0.1 4,246,622 + -2.7 4,246,665 + -1.2 4,246,686 + -0.3 4,246,703 - +0.5 4,246,797 + -2.7 4,246,807 + -0.5 4,246,864 + -2.7 4,246,865 - +0.6 4,246,865 - +0.6 4,246,968 + CA265_RS17035 0.25 -2.2 4,246,969 - CA265_RS17035 0.26 +0.4 4,247,022 + CA265_RS17035 0.35 -2.6 4,247,155 + CA265_RS17035 0.58 -1.0 4,247,209 - CA265_RS17035 0.68 -0.0 4,247,255 + CA265_RS17035 0.76 -1.1 4,247,265 + CA265_RS17035 0.77 -0.4 4,247,279 + CA265_RS17035 0.80 -1.6 4,247,279 + CA265_RS17035 0.80 -1.4 4,247,282 - CA265_RS17035 0.80 +0.4 4,247,283 + CA265_RS17035 0.80 +0.6 4,247,287 + CA265_RS17035 0.81 -1.2 4,247,287 + CA265_RS17035 0.81 -0.7 4,247,293 + CA265_RS17035 0.82 -1.2 4,247,295 + CA265_RS17035 0.83 -2.5 4,247,303 + CA265_RS17035 0.84 -1.2 4,247,303 + CA265_RS17035 0.84 -2.0 4,247,325 - CA265_RS17035 0.88 +0.3 4,247,367 + -0.9 4,247,380 - -1.6 4,247,404 + -1.9 4,247,404 + -1.2 4,247,404 + -1.1 4,247,405 - +0.5 4,247,576 + CA265_RS17040 0.43 -0.9 4,247,598 - CA265_RS17040 0.48 +1.0 4,247,739 + CA265_RS17040 0.79 -1.4 4,247,754 - CA265_RS17040 0.82 +0.8 4,247,776 + CA265_RS17040 0.87 -0.2 4,247,802 - +0.5 4,247,830 + -0.6 4,247,836 + -1.5 4,247,842 + -0.9 4,247,850 + -0.8 4,247,862 + -1.2 4,247,891 + -2.1 4,247,894 - -0.3 4,247,928 + -0.4 4,247,928 + -0.5 4,247,928 + -0.7 4,247,982 + CA265_RS17045 0.10 -2.5 4,247,983 - CA265_RS17045 0.10 -0.1 4,247,983 - CA265_RS17045 0.10 +1.2 4,247,984 + CA265_RS17045 0.10 +0.6 4,247,984 + CA265_RS17045 0.10 +0.0 4,247,984 + CA265_RS17045 0.10 -1.3 4,247,985 - CA265_RS17045 0.10 -0.2 4,247,993 + CA265_RS17045 0.11 -1.4 4,248,004 + CA265_RS17045 0.12 -0.7 4,248,004 + CA265_RS17045 0.12 -1.7 4,248,004 + CA265_RS17045 0.12 -1.1 4,248,004 + CA265_RS17045 0.12 -0.5 4,248,027 - CA265_RS17045 0.13 -0.0 4,248,028 + CA265_RS17045 0.13 -0.5 4,248,060 + CA265_RS17045 0.16 +0.1 4,248,061 - CA265_RS17045 0.16 -0.9 4,248,161 + CA265_RS17045 0.23 -2.9 4,248,162 - CA265_RS17045 0.23 +1.2 4,248,194 + CA265_RS17045 0.25 -0.6 4,248,209 + CA265_RS17045 0.26 -0.1 4,248,241 - CA265_RS17045 0.29 +0.2 4,248,282 - CA265_RS17045 0.31 +1.3 4,248,302 - CA265_RS17045 0.33 +0.8 4,248,319 + CA265_RS17045 0.34 -0.6 4,248,319 + CA265_RS17045 0.34 -0.5 4,248,320 - CA265_RS17045 0.34 +0.2 4,248,325 - CA265_RS17045 0.35 +1.2 4,248,329 + CA265_RS17045 0.35 -0.8 4,248,359 + CA265_RS17045 0.37 -0.6
Or see this region's nucleotide sequence