Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS16780 and CA265_RS16785 are separated by 248 nucleotides CA265_RS16785 and CA265_RS16790 are separated by 105 nucleotides CA265_RS16790 and CA265_RS16795 are separated by 85 nucleotides CA265_RS16795 and CA265_RS16800 are separated by 61 nucleotides
CA265_RS16780: CA265_RS16780 - hypothetical protein, at 4,209,799 to 4,212,768
_RS16780
CA265_RS16785: CA265_RS16785 - 50S ribosomal protein L17, at 4,213,017 to 4,213,541
_RS16785
CA265_RS16790: CA265_RS16790 - DNA-directed RNA polymerase subunit alpha, at 4,213,647 to 4,214,639
_RS16790
CA265_RS16795: CA265_RS16795 - 30S ribosomal protein S4, at 4,214,725 to 4,215,333
_RS16795
CA265_RS16800: CA265_RS16800 - 30S ribosomal protein S11, at 4,215,395 to 4,215,784
_RS16800
Position (kb)
4213
4214
4215 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4212.657 kb on + strand at 4212.687 kb on + strand at 4212.687 kb on + strand at 4212.699 kb on - strand at 4212.718 kb on - strand at 4212.759 kb on + strand at 4212.792 kb on + strand at 4212.812 kb on - strand at 4212.903 kb on + strand at 4212.913 kb on + strand at 4212.913 kb on + strand at 4212.948 kb on - strand at 4212.949 kb on + strand at 4213.004 kb on - strand at 4213.004 kb on - strand at 4213.122 kb on - strand, within CA265_RS16785 at 4214.675 kb on - strand at 4214.711 kb on - strand at 4214.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 4,212,657 + -0.6 4,212,687 + +0.7 4,212,687 + -0.4 4,212,699 - +0.3 4,212,718 - -0.3 4,212,759 + +0.2 4,212,792 + +3.9 4,212,812 - +0.3 4,212,903 + -1.0 4,212,913 + -0.9 4,212,913 + +1.0 4,212,948 - +0.3 4,212,949 + +0.3 4,213,004 - -1.1 4,213,004 - -1.1 4,213,122 - CA265_RS16785 0.20 -1.0 4,214,675 - -0.1 4,214,711 - -0.7 4,214,711 - -2.7
Or see this region's nucleotide sequence