Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12500 and CA265_RS12505 are separated by 98 nucleotides CA265_RS12505 and CA265_RS12510 overlap by 34 nucleotides
CA265_RS12500: CA265_RS12500 - hypothetical protein, at 3,124,193 to 3,126,331
_RS12500
CA265_RS12505: CA265_RS12505 - hypothetical protein, at 3,126,430 to 3,126,786
_RS12505
CA265_RS12510: CA265_RS12510 - hypothetical protein, at 3,126,753 to 3,128,570
_RS12510
Position (kb)
3126
3127 Strain fitness (log2 ratio)
-1
0
1
2 at 3125.433 kb on - strand, within CA265_RS12500 at 3125.442 kb on + strand, within CA265_RS12500 at 3125.443 kb on - strand at 3125.479 kb on + strand, within CA265_RS12500 at 3125.480 kb on - strand, within CA265_RS12500 at 3125.481 kb on + strand, within CA265_RS12500 at 3125.481 kb on + strand, within CA265_RS12500 at 3125.570 kb on - strand, within CA265_RS12500 at 3125.576 kb on + strand, within CA265_RS12500 at 3125.592 kb on + strand, within CA265_RS12500 at 3125.592 kb on + strand, within CA265_RS12500 at 3125.721 kb on - strand, within CA265_RS12500 at 3125.744 kb on - strand, within CA265_RS12500 at 3125.747 kb on - strand, within CA265_RS12500 at 3125.873 kb on - strand, within CA265_RS12500 at 3125.941 kb on + strand, within CA265_RS12500 at 3125.992 kb on + strand, within CA265_RS12500 at 3125.993 kb on - strand, within CA265_RS12500 at 3125.999 kb on - strand, within CA265_RS12500 at 3126.002 kb on + strand, within CA265_RS12500 at 3126.065 kb on + strand, within CA265_RS12500 at 3126.065 kb on + strand, within CA265_RS12500 at 3126.090 kb on + strand, within CA265_RS12500 at 3126.091 kb on - strand, within CA265_RS12500 at 3126.116 kb on - strand, within CA265_RS12500 at 3126.127 kb on + strand at 3126.165 kb on + strand at 3126.233 kb on + strand at 3126.279 kb on - strand at 3126.322 kb on + strand at 3126.331 kb on + strand at 3126.331 kb on + strand at 3126.384 kb on + strand at 3126.431 kb on + strand at 3126.434 kb on - strand at 3126.443 kb on - strand at 3126.518 kb on - strand, within CA265_RS12505 at 3126.628 kb on + strand, within CA265_RS12505 at 3126.708 kb on - strand, within CA265_RS12505 at 3126.816 kb on + strand at 3126.816 kb on + strand at 3126.816 kb on + strand at 3126.816 kb on + strand at 3126.817 kb on - strand at 3126.817 kb on - strand at 3126.836 kb on - strand at 3126.848 kb on + strand at 3126.849 kb on - strand at 3126.855 kb on - strand at 3126.855 kb on - strand at 3126.869 kb on - strand at 3126.985 kb on - strand, within CA265_RS12510 at 3127.005 kb on - strand, within CA265_RS12510 at 3127.026 kb on + strand, within CA265_RS12510 at 3127.027 kb on - strand, within CA265_RS12510 at 3127.027 kb on - strand, within CA265_RS12510 at 3127.061 kb on + strand, within CA265_RS12510 at 3127.078 kb on - strand, within CA265_RS12510 at 3127.125 kb on + strand, within CA265_RS12510 at 3127.323 kb on - strand, within CA265_RS12510 at 3127.376 kb on + strand, within CA265_RS12510 at 3127.378 kb on + strand, within CA265_RS12510 at 3127.452 kb on + strand, within CA265_RS12510 at 3127.528 kb on + strand, within CA265_RS12510 at 3127.548 kb on + strand, within CA265_RS12510 at 3127.634 kb on + strand, within CA265_RS12510 at 3127.635 kb on - strand, within CA265_RS12510 at 3127.671 kb on + strand, within CA265_RS12510 at 3127.679 kb on - strand, within CA265_RS12510 at 3127.681 kb on + strand, within CA265_RS12510
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 3,125,433 - CA265_RS12500 0.58 -0.6 3,125,442 + CA265_RS12500 0.58 -0.1 3,125,443 - -0.2 3,125,479 + CA265_RS12500 0.60 -0.4 3,125,480 - CA265_RS12500 0.60 +0.2 3,125,481 + CA265_RS12500 0.60 -0.0 3,125,481 + CA265_RS12500 0.60 +0.2 3,125,570 - CA265_RS12500 0.64 -0.1 3,125,576 + CA265_RS12500 0.65 -0.3 3,125,592 + CA265_RS12500 0.65 -0.3 3,125,592 + CA265_RS12500 0.65 -0.2 3,125,721 - CA265_RS12500 0.71 -0.1 3,125,744 - CA265_RS12500 0.73 +0.2 3,125,747 - CA265_RS12500 0.73 -0.5 3,125,873 - CA265_RS12500 0.79 -0.9 3,125,941 + CA265_RS12500 0.82 -0.2 3,125,992 + CA265_RS12500 0.84 +0.8 3,125,993 - CA265_RS12500 0.84 -0.6 3,125,999 - CA265_RS12500 0.84 -0.4 3,126,002 + CA265_RS12500 0.85 -0.1 3,126,065 + CA265_RS12500 0.88 +0.2 3,126,065 + CA265_RS12500 0.88 -0.3 3,126,090 + CA265_RS12500 0.89 -0.4 3,126,091 - CA265_RS12500 0.89 -0.1 3,126,116 - CA265_RS12500 0.90 -0.4 3,126,127 + +0.7 3,126,165 + -0.1 3,126,233 + +0.7 3,126,279 - +0.6 3,126,322 + -0.7 3,126,331 + +0.2 3,126,331 + -0.5 3,126,384 + +0.4 3,126,431 + +0.4 3,126,434 - -1.0 3,126,443 - -0.6 3,126,518 - CA265_RS12505 0.25 -1.2 3,126,628 + CA265_RS12505 0.55 -0.1 3,126,708 - CA265_RS12505 0.78 -0.7 3,126,816 + +2.4 3,126,816 + +0.1 3,126,816 + -0.2 3,126,816 + -0.4 3,126,817 - +0.2 3,126,817 - -0.4 3,126,836 - -0.1 3,126,848 + -0.3 3,126,849 - +0.7 3,126,855 - +0.3 3,126,855 - -0.4 3,126,869 - -0.3 3,126,985 - CA265_RS12510 0.13 -0.4 3,127,005 - CA265_RS12510 0.14 -1.1 3,127,026 + CA265_RS12510 0.15 +0.4 3,127,027 - CA265_RS12510 0.15 -0.4 3,127,027 - CA265_RS12510 0.15 +0.0 3,127,061 + CA265_RS12510 0.17 +0.1 3,127,078 - CA265_RS12510 0.18 +0.3 3,127,125 + CA265_RS12510 0.20 +1.7 3,127,323 - CA265_RS12510 0.31 -1.0 3,127,376 + CA265_RS12510 0.34 +1.2 3,127,378 + CA265_RS12510 0.34 +0.7 3,127,452 + CA265_RS12510 0.38 -0.3 3,127,528 + CA265_RS12510 0.43 +0.2 3,127,548 + CA265_RS12510 0.44 -0.4 3,127,634 + CA265_RS12510 0.48 -0.1 3,127,635 - CA265_RS12510 0.49 -1.0 3,127,671 + CA265_RS12510 0.50 -0.3 3,127,679 - CA265_RS12510 0.51 +0.4 3,127,681 + CA265_RS12510 0.51 +0.4
Or see this region's nucleotide sequence