Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12315 and CA265_RS12320 are separated by 308 nucleotides CA265_RS12320 and CA265_RS12325 are separated by 375 nucleotides CA265_RS12325 and CA265_RS12330 are separated by 163 nucleotides
CA265_RS12315: CA265_RS12315 - grasp-with-spasm system ATP-grasp peptide maturase, at 3,070,752 to 3,071,684
_RS12315
CA265_RS12320: CA265_RS12320 - hypothetical protein, at 3,071,993 to 3,072,265
_RS12320
CA265_RS12325: CA265_RS12325 - hypothetical protein, at 3,072,641 to 3,073,063
_RS12325
CA265_RS12330: CA265_RS12330 - response regulator, at 3,073,227 to 3,073,598
_RS12330
Position (kb)
3071
3072
3073 Strain fitness (log2 ratio)
-1
0
1
2 at 3070.998 kb on + strand, within CA265_RS12315 at 3071.007 kb on - strand, within CA265_RS12315 at 3071.059 kb on + strand, within CA265_RS12315 at 3071.222 kb on + strand, within CA265_RS12315 at 3071.248 kb on + strand, within CA265_RS12315 at 3071.303 kb on + strand at 3071.320 kb on - strand, within CA265_RS12315 at 3071.367 kb on + strand, within CA265_RS12315 at 3071.368 kb on - strand, within CA265_RS12315 at 3071.370 kb on - strand, within CA265_RS12315 at 3071.370 kb on - strand, within CA265_RS12315 at 3071.396 kb on - strand, within CA265_RS12315 at 3071.402 kb on - strand, within CA265_RS12315 at 3071.434 kb on + strand, within CA265_RS12315 at 3071.434 kb on + strand, within CA265_RS12315 at 3071.435 kb on - strand, within CA265_RS12315 at 3071.474 kb on - strand, within CA265_RS12315 at 3071.528 kb on + strand, within CA265_RS12315 at 3071.643 kb on - strand at 3071.684 kb on + strand at 3071.776 kb on + strand at 3071.777 kb on - strand at 3071.795 kb on + strand at 3071.856 kb on + strand at 3071.857 kb on - strand at 3071.973 kb on + strand at 3071.973 kb on + strand at 3071.974 kb on - strand at 3071.975 kb on + strand at 3071.975 kb on + strand at 3071.979 kb on + strand at 3071.980 kb on - strand at 3072.047 kb on - strand, within CA265_RS12320 at 3072.055 kb on - strand, within CA265_RS12320 at 3072.098 kb on + strand, within CA265_RS12320 at 3072.130 kb on + strand, within CA265_RS12320 at 3072.175 kb on + strand, within CA265_RS12320 at 3072.185 kb on - strand, within CA265_RS12320 at 3072.289 kb on + strand at 3072.289 kb on + strand at 3072.520 kb on - strand at 3072.586 kb on + strand at 3072.587 kb on - strand at 3072.609 kb on + strand at 3072.652 kb on - strand at 3072.683 kb on - strand at 3072.683 kb on - strand at 3072.735 kb on + strand, within CA265_RS12325 at 3072.735 kb on + strand, within CA265_RS12325 at 3072.766 kb on + strand, within CA265_RS12325 at 3072.779 kb on + strand, within CA265_RS12325 at 3072.780 kb on - strand, within CA265_RS12325 at 3072.780 kb on - strand, within CA265_RS12325 at 3072.820 kb on + strand, within CA265_RS12325 at 3072.821 kb on - strand, within CA265_RS12325 at 3072.821 kb on - strand, within CA265_RS12325 at 3072.834 kb on - strand, within CA265_RS12325 at 3072.878 kb on - strand, within CA265_RS12325 at 3072.892 kb on + strand, within CA265_RS12325 at 3072.899 kb on - strand, within CA265_RS12325 at 3072.963 kb on + strand, within CA265_RS12325 at 3072.964 kb on - strand, within CA265_RS12325 at 3072.993 kb on + strand, within CA265_RS12325 at 3072.994 kb on - strand, within CA265_RS12325 at 3072.995 kb on + strand, within CA265_RS12325 at 3073.001 kb on - strand at 3073.065 kb on + strand at 3073.066 kb on - strand at 3073.099 kb on - strand at 3073.109 kb on + strand at 3073.109 kb on + strand at 3073.109 kb on + strand at 3073.121 kb on - strand at 3073.136 kb on + strand at 3073.216 kb on + strand at 3073.216 kb on + strand at 3073.218 kb on + strand at 3073.218 kb on + strand at 3073.219 kb on - strand at 3073.219 kb on - strand at 3073.238 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 3,070,998 + CA265_RS12315 0.26 +0.0 3,071,007 - CA265_RS12315 0.27 -0.3 3,071,059 + CA265_RS12315 0.33 -0.3 3,071,222 + CA265_RS12315 0.50 +0.2 3,071,248 + CA265_RS12315 0.53 -0.5 3,071,303 + +2.4 3,071,320 - CA265_RS12315 0.61 -0.4 3,071,367 + CA265_RS12315 0.66 +0.0 3,071,368 - CA265_RS12315 0.66 +0.6 3,071,370 - CA265_RS12315 0.66 -0.3 3,071,370 - CA265_RS12315 0.66 -0.4 3,071,396 - CA265_RS12315 0.69 +0.0 3,071,402 - CA265_RS12315 0.70 +0.2 3,071,434 + CA265_RS12315 0.73 -0.8 3,071,434 + CA265_RS12315 0.73 -0.0 3,071,435 - CA265_RS12315 0.73 +0.6 3,071,474 - CA265_RS12315 0.77 -0.1 3,071,528 + CA265_RS12315 0.83 +0.1 3,071,643 - -0.3 3,071,684 + -1.1 3,071,776 + -0.2 3,071,777 - -0.9 3,071,795 + +0.1 3,071,856 + -0.1 3,071,857 - +0.1 3,071,973 + +0.3 3,071,973 + +0.1 3,071,974 - -0.8 3,071,975 + +0.9 3,071,975 + +0.6 3,071,979 + -0.1 3,071,980 - -1.3 3,072,047 - CA265_RS12320 0.20 -0.6 3,072,055 - CA265_RS12320 0.23 -0.7 3,072,098 + CA265_RS12320 0.38 +0.2 3,072,130 + CA265_RS12320 0.50 -0.8 3,072,175 + CA265_RS12320 0.67 +0.1 3,072,185 - CA265_RS12320 0.70 -0.4 3,072,289 + -0.1 3,072,289 + +0.5 3,072,520 - +0.1 3,072,586 + +0.5 3,072,587 - +0.1 3,072,609 + +0.8 3,072,652 - +0.5 3,072,683 - -0.1 3,072,683 - +0.4 3,072,735 + CA265_RS12325 0.22 +0.4 3,072,735 + CA265_RS12325 0.22 -0.1 3,072,766 + CA265_RS12325 0.30 +2.1 3,072,779 + CA265_RS12325 0.33 +0.4 3,072,780 - CA265_RS12325 0.33 -0.0 3,072,780 - CA265_RS12325 0.33 +0.5 3,072,820 + CA265_RS12325 0.42 +0.5 3,072,821 - CA265_RS12325 0.43 +0.1 3,072,821 - CA265_RS12325 0.43 -0.1 3,072,834 - CA265_RS12325 0.46 -0.2 3,072,878 - CA265_RS12325 0.56 +0.2 3,072,892 + CA265_RS12325 0.59 -0.5 3,072,899 - CA265_RS12325 0.61 -0.0 3,072,963 + CA265_RS12325 0.76 +0.8 3,072,964 - CA265_RS12325 0.76 +0.9 3,072,993 + CA265_RS12325 0.83 +0.4 3,072,994 - CA265_RS12325 0.83 +0.2 3,072,995 + CA265_RS12325 0.84 +0.6 3,073,001 - +0.4 3,073,065 + -0.3 3,073,066 - +0.6 3,073,099 - +0.2 3,073,109 + +0.2 3,073,109 + +0.4 3,073,109 + +1.1 3,073,121 - -1.4 3,073,136 + -0.1 3,073,216 + -1.0 3,073,216 + +0.9 3,073,218 + +0.4 3,073,218 + -0.0 3,073,219 - -0.4 3,073,219 - -0.7 3,073,238 + -0.3
Or see this region's nucleotide sequence