Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02130 and CA265_RS02135 are separated by 196 nucleotides CA265_RS02135 and CA265_RS02140 are separated by 442 nucleotides
CA265_RS02130: CA265_RS02130 - penicillin acylase family protein, at 533,023 to 535,452
_RS02130
CA265_RS02135: CA265_RS02135 - TonB-dependent receptor, at 535,649 to 538,540
_RS02135
CA265_RS02140: CA265_RS02140 - glycosyl hydrolase, at 538,983 to 541,445
_RS02140
Position (kb) 535
536
537
538
539 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 534.827 kb on + strand, within CA265_RS02130 at 534.850 kb on + strand, within CA265_RS02130 at 534.851 kb on - strand, within CA265_RS02130 at 534.854 kb on - strand, within CA265_RS02130 at 534.854 kb on - strand, within CA265_RS02130 at 534.930 kb on - strand, within CA265_RS02130 at 534.935 kb on - strand, within CA265_RS02130 at 534.935 kb on - strand, within CA265_RS02130 at 534.966 kb on + strand, within CA265_RS02130 at 534.969 kb on - strand, within CA265_RS02130 at 534.989 kb on + strand, within CA265_RS02130 at 534.990 kb on - strand, within CA265_RS02130 at 535.055 kb on + strand, within CA265_RS02130 at 535.102 kb on - strand, within CA265_RS02130 at 535.102 kb on - strand, within CA265_RS02130 at 535.181 kb on - strand, within CA265_RS02130 at 535.184 kb on + strand, within CA265_RS02130 at 535.199 kb on + strand, within CA265_RS02130 at 535.200 kb on - strand, within CA265_RS02130 at 535.228 kb on - strand at 535.271 kb on - strand at 535.271 kb on - strand at 535.351 kb on + strand at 535.374 kb on + strand at 535.375 kb on - strand at 535.447 kb on - strand at 535.502 kb on - strand at 535.532 kb on - strand at 535.612 kb on + strand at 535.684 kb on - strand at 535.727 kb on - strand at 535.729 kb on + strand at 535.754 kb on - strand at 535.788 kb on + strand at 535.788 kb on + strand at 535.793 kb on - strand at 535.847 kb on - strand at 535.876 kb on - strand at 535.887 kb on - strand at 535.905 kb on + strand at 535.983 kb on + strand, within CA265_RS02135 at 535.984 kb on - strand, within CA265_RS02135 at 536.000 kb on - strand, within CA265_RS02135 at 536.000 kb on - strand, within CA265_RS02135 at 536.013 kb on + strand, within CA265_RS02135 at 536.056 kb on + strand, within CA265_RS02135 at 536.101 kb on + strand, within CA265_RS02135 at 536.122 kb on + strand, within CA265_RS02135 at 536.123 kb on - strand, within CA265_RS02135 at 536.153 kb on - strand, within CA265_RS02135 at 536.177 kb on - strand, within CA265_RS02135 at 536.180 kb on - strand, within CA265_RS02135 at 536.245 kb on + strand, within CA265_RS02135 at 536.274 kb on + strand, within CA265_RS02135 at 536.274 kb on + strand, within CA265_RS02135 at 536.274 kb on + strand, within CA265_RS02135 at 536.275 kb on - strand, within CA265_RS02135 at 536.278 kb on + strand, within CA265_RS02135 at 536.329 kb on + strand, within CA265_RS02135 at 536.329 kb on + strand, within CA265_RS02135 at 536.369 kb on - strand, within CA265_RS02135 at 536.539 kb on + strand, within CA265_RS02135 at 536.543 kb on - strand, within CA265_RS02135 at 536.543 kb on - strand, within CA265_RS02135 at 536.543 kb on - strand, within CA265_RS02135 at 536.546 kb on + strand, within CA265_RS02135 at 536.546 kb on + strand, within CA265_RS02135 at 536.547 kb on - strand, within CA265_RS02135 at 536.766 kb on + strand, within CA265_RS02135 at 536.767 kb on - strand, within CA265_RS02135 at 536.797 kb on - strand, within CA265_RS02135 at 536.829 kb on - strand, within CA265_RS02135 at 536.872 kb on + strand, within CA265_RS02135 at 536.872 kb on + strand, within CA265_RS02135 at 536.873 kb on - strand, within CA265_RS02135 at 536.937 kb on + strand, within CA265_RS02135 at 536.942 kb on + strand, within CA265_RS02135 at 537.095 kb on + strand, within CA265_RS02135 at 537.126 kb on - strand, within CA265_RS02135 at 537.151 kb on + strand, within CA265_RS02135 at 537.152 kb on - strand, within CA265_RS02135 at 537.155 kb on - strand, within CA265_RS02135 at 537.197 kb on + strand, within CA265_RS02135 at 537.198 kb on - strand, within CA265_RS02135 at 537.311 kb on - strand, within CA265_RS02135 at 537.313 kb on + strand, within CA265_RS02135 at 537.320 kb on - strand, within CA265_RS02135 at 537.329 kb on - strand, within CA265_RS02135 at 537.330 kb on + strand, within CA265_RS02135 at 537.373 kb on - strand, within CA265_RS02135 at 537.374 kb on + strand, within CA265_RS02135 at 537.445 kb on + strand, within CA265_RS02135 at 537.453 kb on + strand at 537.454 kb on - strand, within CA265_RS02135 at 537.495 kb on + strand, within CA265_RS02135 at 537.496 kb on - strand, within CA265_RS02135 at 537.539 kb on - strand, within CA265_RS02135 at 537.551 kb on + strand, within CA265_RS02135 at 537.623 kb on - strand, within CA265_RS02135 at 537.658 kb on + strand, within CA265_RS02135 at 537.688 kb on + strand, within CA265_RS02135 at 537.746 kb on + strand, within CA265_RS02135 at 537.746 kb on + strand, within CA265_RS02135 at 537.784 kb on - strand, within CA265_RS02135 at 537.793 kb on + strand, within CA265_RS02135 at 537.794 kb on - strand, within CA265_RS02135 at 537.794 kb on - strand, within CA265_RS02135 at 537.795 kb on + strand, within CA265_RS02135 at 537.796 kb on - strand, within CA265_RS02135 at 537.812 kb on - strand, within CA265_RS02135 at 537.815 kb on + strand, within CA265_RS02135 at 537.847 kb on + strand, within CA265_RS02135 at 537.847 kb on + strand, within CA265_RS02135 at 537.869 kb on + strand, within CA265_RS02135 at 537.920 kb on + strand, within CA265_RS02135 at 537.966 kb on - strand, within CA265_RS02135 at 537.979 kb on + strand, within CA265_RS02135 at 537.991 kb on - strand, within CA265_RS02135 at 538.017 kb on - strand, within CA265_RS02135 at 538.022 kb on + strand, within CA265_RS02135 at 538.022 kb on + strand, within CA265_RS02135 at 538.023 kb on - strand, within CA265_RS02135 at 538.025 kb on + strand, within CA265_RS02135 at 538.025 kb on + strand, within CA265_RS02135 at 538.026 kb on - strand, within CA265_RS02135 at 538.026 kb on - strand, within CA265_RS02135 at 538.035 kb on - strand, within CA265_RS02135 at 538.079 kb on - strand, within CA265_RS02135 at 538.092 kb on - strand, within CA265_RS02135 at 538.095 kb on + strand, within CA265_RS02135 at 538.132 kb on + strand, within CA265_RS02135 at 538.133 kb on - strand, within CA265_RS02135 at 538.152 kb on + strand, within CA265_RS02135 at 538.175 kb on - strand, within CA265_RS02135 at 538.198 kb on - strand, within CA265_RS02135 at 538.198 kb on - strand, within CA265_RS02135 at 538.202 kb on + strand, within CA265_RS02135 at 538.203 kb on - strand, within CA265_RS02135 at 538.229 kb on - strand, within CA265_RS02135 at 538.232 kb on + strand, within CA265_RS02135 at 538.232 kb on + strand, within CA265_RS02135 at 538.234 kb on + strand, within CA265_RS02135 at 538.235 kb on - strand, within CA265_RS02135 at 538.268 kb on - strand at 538.268 kb on - strand at 538.268 kb on - strand at 538.268 kb on - strand at 538.322 kb on + strand at 538.392 kb on + strand at 538.393 kb on - strand at 538.393 kb on - strand at 538.425 kb on + strand at 538.457 kb on - strand at 538.518 kb on + strand at 538.526 kb on - strand at 538.539 kb on - strand at 538.568 kb on + strand at 538.568 kb on + strand at 538.580 kb on - strand at 538.580 kb on - strand at 538.582 kb on - strand at 538.647 kb on - strand at 538.671 kb on - strand at 538.682 kb on - strand at 538.687 kb on + strand at 538.688 kb on - strand at 538.738 kb on + strand at 538.739 kb on - strand at 538.747 kb on + strand at 538.784 kb on + strand at 538.813 kb on + strand at 538.814 kb on - strand at 538.916 kb on + strand at 538.916 kb on + strand at 538.920 kb on - strand at 538.932 kb on + strand at 538.978 kb on + strand at 538.978 kb on + strand at 538.978 kb on + strand at 539.053 kb on + strand at 539.053 kb on + strand at 539.066 kb on - strand at 539.070 kb on - strand at 539.168 kb on - strand at 539.192 kb on + strand at 539.193 kb on - strand at 539.193 kb on - strand at 539.318 kb on + strand, within CA265_RS02140 at 539.318 kb on + strand, within CA265_RS02140 at 539.375 kb on + strand, within CA265_RS02140 at 539.389 kb on + strand, within CA265_RS02140 at 539.389 kb on + strand, within CA265_RS02140 at 539.393 kb on + strand, within CA265_RS02140 at 539.393 kb on + strand, within CA265_RS02140 at 539.414 kb on + strand, within CA265_RS02140 at 539.453 kb on + strand, within CA265_RS02140 at 539.457 kb on + strand, within CA265_RS02140 at 539.458 kb on - strand, within CA265_RS02140 at 539.501 kb on - strand, within CA265_RS02140 at 539.501 kb on - strand, within CA265_RS02140 at 539.507 kb on + strand, within CA265_RS02140
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 534,827 + CA265_RS02130 0.74 +0.9 534,850 + CA265_RS02130 0.75 +0.3 534,851 - CA265_RS02130 0.75 -0.9 534,854 - CA265_RS02130 0.75 +0.2 534,854 - CA265_RS02130 0.75 -0.3 534,930 - CA265_RS02130 0.78 -0.0 534,935 - CA265_RS02130 0.79 +0.9 534,935 - CA265_RS02130 0.79 +0.5 534,966 + CA265_RS02130 0.80 +1.0 534,969 - CA265_RS02130 0.80 -0.5 534,989 + CA265_RS02130 0.81 -0.7 534,990 - CA265_RS02130 0.81 +0.6 535,055 + CA265_RS02130 0.84 -0.1 535,102 - CA265_RS02130 0.86 -0.5 535,102 - CA265_RS02130 0.86 -0.9 535,181 - CA265_RS02130 0.89 -0.4 535,184 + CA265_RS02130 0.89 -0.4 535,199 + CA265_RS02130 0.90 +0.2 535,200 - CA265_RS02130 0.90 -0.1 535,228 - -0.2 535,271 - -0.9 535,271 - +0.4 535,351 + +2.1 535,374 + +0.5 535,375 - -0.6 535,447 - -2.5 535,502 - +0.5 535,532 - -0.5 535,612 + -0.3 535,684 - +1.8 535,727 - +0.1 535,729 + +1.2 535,754 - -0.7 535,788 + +0.1 535,788 + -0.0 535,793 - -0.4 535,847 - +0.5 535,876 - +0.3 535,887 - -0.2 535,905 + -0.3 535,983 + CA265_RS02135 0.12 -1.2 535,984 - CA265_RS02135 0.12 -0.1 536,000 - CA265_RS02135 0.12 +0.1 536,000 - CA265_RS02135 0.12 -0.2 536,013 + CA265_RS02135 0.13 +0.2 536,056 + CA265_RS02135 0.14 +0.7 536,101 + CA265_RS02135 0.16 -0.3 536,122 + CA265_RS02135 0.16 -0.3 536,123 - CA265_RS02135 0.16 -0.6 536,153 - CA265_RS02135 0.17 +0.0 536,177 - CA265_RS02135 0.18 +0.8 536,180 - CA265_RS02135 0.18 -3.2 536,245 + CA265_RS02135 0.21 -1.4 536,274 + CA265_RS02135 0.22 -1.4 536,274 + CA265_RS02135 0.22 -0.3 536,274 + CA265_RS02135 0.22 -0.2 536,275 - CA265_RS02135 0.22 -0.5 536,278 + CA265_RS02135 0.22 -0.1 536,329 + CA265_RS02135 0.24 -0.1 536,329 + CA265_RS02135 0.24 -0.8 536,369 - CA265_RS02135 0.25 +0.1 536,539 + CA265_RS02135 0.31 -0.1 536,543 - CA265_RS02135 0.31 -2.1 536,543 - CA265_RS02135 0.31 +0.0 536,543 - CA265_RS02135 0.31 +0.0 536,546 + CA265_RS02135 0.31 -0.7 536,546 + CA265_RS02135 0.31 -0.0 536,547 - CA265_RS02135 0.31 -1.0 536,766 + CA265_RS02135 0.39 +0.8 536,767 - CA265_RS02135 0.39 +0.5 536,797 - CA265_RS02135 0.40 -0.4 536,829 - CA265_RS02135 0.41 +0.3 536,872 + CA265_RS02135 0.42 +0.4 536,872 + CA265_RS02135 0.42 -1.2 536,873 - CA265_RS02135 0.42 -0.7 536,937 + CA265_RS02135 0.45 -0.4 536,942 + CA265_RS02135 0.45 +0.2 537,095 + CA265_RS02135 0.50 +0.1 537,126 - CA265_RS02135 0.51 +0.2 537,151 + CA265_RS02135 0.52 +1.5 537,152 - CA265_RS02135 0.52 -0.7 537,155 - CA265_RS02135 0.52 -0.2 537,197 + CA265_RS02135 0.54 -0.4 537,198 - CA265_RS02135 0.54 +0.3 537,311 - CA265_RS02135 0.57 +0.4 537,313 + CA265_RS02135 0.58 -0.5 537,320 - CA265_RS02135 0.58 -0.0 537,329 - CA265_RS02135 0.58 -0.0 537,330 + CA265_RS02135 0.58 -0.2 537,373 - CA265_RS02135 0.60 -0.9 537,374 + CA265_RS02135 0.60 -0.2 537,445 + CA265_RS02135 0.62 -0.2 537,453 + +2.0 537,454 - CA265_RS02135 0.62 +0.0 537,495 + CA265_RS02135 0.64 +0.6 537,496 - CA265_RS02135 0.64 -0.1 537,539 - CA265_RS02135 0.65 -0.2 537,551 + CA265_RS02135 0.66 -1.9 537,623 - CA265_RS02135 0.68 -1.4 537,658 + CA265_RS02135 0.69 -0.2 537,688 + CA265_RS02135 0.71 -0.0 537,746 + CA265_RS02135 0.73 -1.1 537,746 + CA265_RS02135 0.73 -0.1 537,784 - CA265_RS02135 0.74 -0.2 537,793 + CA265_RS02135 0.74 -1.1 537,794 - CA265_RS02135 0.74 +0.6 537,794 - CA265_RS02135 0.74 +0.3 537,795 + CA265_RS02135 0.74 -0.3 537,796 - CA265_RS02135 0.74 +0.6 537,812 - CA265_RS02135 0.75 -1.0 537,815 + CA265_RS02135 0.75 +0.6 537,847 + CA265_RS02135 0.76 -0.2 537,847 + CA265_RS02135 0.76 -0.0 537,869 + CA265_RS02135 0.77 +1.8 537,920 + CA265_RS02135 0.79 +0.1 537,966 - CA265_RS02135 0.80 -0.4 537,979 + CA265_RS02135 0.81 -2.2 537,991 - CA265_RS02135 0.81 +0.4 538,017 - CA265_RS02135 0.82 -0.5 538,022 + CA265_RS02135 0.82 -1.6 538,022 + CA265_RS02135 0.82 +0.1 538,023 - CA265_RS02135 0.82 -0.2 538,025 + CA265_RS02135 0.82 +0.3 538,025 + CA265_RS02135 0.82 -1.2 538,026 - CA265_RS02135 0.82 -0.5 538,026 - CA265_RS02135 0.82 +0.2 538,035 - CA265_RS02135 0.83 -0.2 538,079 - CA265_RS02135 0.84 -0.4 538,092 - CA265_RS02135 0.84 -0.1 538,095 + CA265_RS02135 0.85 +0.5 538,132 + CA265_RS02135 0.86 -0.5 538,133 - CA265_RS02135 0.86 +0.8 538,152 + CA265_RS02135 0.87 -0.1 538,175 - CA265_RS02135 0.87 +0.2 538,198 - CA265_RS02135 0.88 +0.2 538,198 - CA265_RS02135 0.88 -0.5 538,202 + CA265_RS02135 0.88 +0.4 538,203 - CA265_RS02135 0.88 -0.3 538,229 - CA265_RS02135 0.89 +0.2 538,232 + CA265_RS02135 0.89 -0.0 538,232 + CA265_RS02135 0.89 -0.3 538,234 + CA265_RS02135 0.89 +2.0 538,235 - CA265_RS02135 0.89 +0.0 538,268 - +0.4 538,268 - -0.7 538,268 - -0.5 538,268 - -0.5 538,322 + +0.6 538,392 + -0.3 538,393 - -0.6 538,393 - -0.1 538,425 + -0.1 538,457 - -0.6 538,518 + -0.1 538,526 - +0.3 538,539 - -0.2 538,568 + -0.4 538,568 + +0.2 538,580 - -0.3 538,580 - -0.0 538,582 - -0.7 538,647 - -0.2 538,671 - -0.4 538,682 - -0.1 538,687 + -0.7 538,688 - +0.2 538,738 + +0.2 538,739 - +0.4 538,747 + -0.4 538,784 + +0.4 538,813 + -0.2 538,814 - +0.4 538,916 + +0.2 538,916 + +0.5 538,920 - -0.5 538,932 + +0.8 538,978 + +0.2 538,978 + -0.5 538,978 + -0.5 539,053 + -0.5 539,053 + +0.0 539,066 - -0.7 539,070 - -0.6 539,168 - -0.6 539,192 + -0.1 539,193 - -0.5 539,193 - -0.9 539,318 + CA265_RS02140 0.14 -1.1 539,318 + CA265_RS02140 0.14 +0.3 539,375 + CA265_RS02140 0.16 -0.6 539,389 + CA265_RS02140 0.16 -0.7 539,389 + CA265_RS02140 0.16 -1.1 539,393 + CA265_RS02140 0.17 -0.2 539,393 + CA265_RS02140 0.17 -0.5 539,414 + CA265_RS02140 0.17 -0.4 539,453 + CA265_RS02140 0.19 -0.7 539,457 + CA265_RS02140 0.19 +0.2 539,458 - CA265_RS02140 0.19 -1.3 539,501 - CA265_RS02140 0.21 -0.6 539,501 - CA265_RS02140 0.21 +0.3 539,507 + CA265_RS02140 0.21 -0.1
Or see this region's nucleotide sequence