Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02120 and CA265_RS02125 are separated by 4 nucleotides CA265_RS02125 and CA265_RS02130 are separated by 6 nucleotides CA265_RS02130 and CA265_RS02135 are separated by 196 nucleotides
CA265_RS02120: CA265_RS02120 - acyl transferase, at 531,654 to 532,652
_RS02120
CA265_RS02125: CA265_RS02125 - hypothetical protein, at 532,657 to 533,016
_RS02125
CA265_RS02130: CA265_RS02130 - penicillin acylase family protein, at 533,023 to 535,452
_RS02130
CA265_RS02135: CA265_RS02135 - TonB-dependent receptor, at 535,649 to 538,540
_RS02135
Position (kb)
533
534
535
536 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 532.076 kb on + strand, within CA265_RS02120 at 532.076 kb on + strand, within CA265_RS02120 at 532.094 kb on - strand, within CA265_RS02120 at 532.169 kb on + strand, within CA265_RS02120 at 532.174 kb on - strand, within CA265_RS02120 at 532.227 kb on - strand, within CA265_RS02120 at 532.228 kb on + strand, within CA265_RS02120 at 532.229 kb on - strand, within CA265_RS02120 at 532.327 kb on + strand, within CA265_RS02120 at 532.327 kb on + strand, within CA265_RS02120 at 532.357 kb on - strand, within CA265_RS02120 at 532.357 kb on - strand, within CA265_RS02120 at 532.385 kb on + strand, within CA265_RS02120 at 532.424 kb on + strand, within CA265_RS02120 at 532.513 kb on - strand, within CA265_RS02120 at 532.526 kb on - strand, within CA265_RS02120 at 532.558 kb on - strand at 532.615 kb on - strand at 532.622 kb on + strand at 532.622 kb on + strand at 532.623 kb on - strand at 532.623 kb on - strand at 532.649 kb on - strand at 532.649 kb on - strand at 532.658 kb on + strand at 532.718 kb on + strand, within CA265_RS02125 at 532.819 kb on + strand, within CA265_RS02125 at 532.831 kb on - strand, within CA265_RS02125 at 532.846 kb on + strand, within CA265_RS02125 at 532.847 kb on - strand, within CA265_RS02125 at 532.994 kb on - strand at 532.996 kb on + strand at 533.001 kb on + strand at 533.019 kb on + strand at 533.050 kb on - strand at 533.104 kb on - strand at 533.128 kb on - strand at 533.131 kb on - strand at 533.136 kb on - strand at 533.246 kb on - strand at 533.253 kb on + strand at 533.253 kb on + strand at 533.254 kb on - strand at 533.254 kb on - strand at 533.256 kb on + strand at 533.257 kb on - strand at 533.324 kb on - strand, within CA265_RS02130 at 533.387 kb on - strand, within CA265_RS02130 at 533.392 kb on - strand, within CA265_RS02130 at 533.431 kb on + strand, within CA265_RS02130 at 533.431 kb on + strand, within CA265_RS02130 at 533.569 kb on - strand, within CA265_RS02130 at 533.651 kb on + strand, within CA265_RS02130 at 533.652 kb on - strand, within CA265_RS02130 at 533.733 kb on + strand, within CA265_RS02130 at 533.741 kb on + strand, within CA265_RS02130 at 533.784 kb on + strand, within CA265_RS02130 at 533.784 kb on + strand, within CA265_RS02130 at 533.828 kb on + strand, within CA265_RS02130 at 533.847 kb on + strand, within CA265_RS02130 at 533.853 kb on - strand, within CA265_RS02130 at 533.853 kb on - strand, within CA265_RS02130 at 533.856 kb on - strand, within CA265_RS02130 at 533.919 kb on + strand, within CA265_RS02130 at 533.988 kb on + strand, within CA265_RS02130 at 534.051 kb on + strand, within CA265_RS02130 at 534.052 kb on - strand, within CA265_RS02130 at 534.055 kb on + strand, within CA265_RS02130 at 534.055 kb on + strand, within CA265_RS02130 at 534.056 kb on - strand, within CA265_RS02130 at 534.148 kb on + strand at 534.208 kb on + strand, within CA265_RS02130 at 534.247 kb on + strand, within CA265_RS02130 at 534.248 kb on - strand, within CA265_RS02130 at 534.270 kb on - strand, within CA265_RS02130 at 534.272 kb on + strand, within CA265_RS02130 at 534.273 kb on - strand, within CA265_RS02130 at 534.284 kb on - strand, within CA265_RS02130 at 534.305 kb on - strand, within CA265_RS02130 at 534.345 kb on + strand, within CA265_RS02130 at 534.346 kb on - strand, within CA265_RS02130 at 534.346 kb on - strand, within CA265_RS02130 at 534.351 kb on + strand, within CA265_RS02130 at 534.354 kb on - strand, within CA265_RS02130 at 534.354 kb on - strand, within CA265_RS02130 at 534.494 kb on + strand, within CA265_RS02130 at 534.498 kb on + strand, within CA265_RS02130 at 534.499 kb on - strand, within CA265_RS02130 at 534.499 kb on - strand, within CA265_RS02130 at 534.601 kb on - strand, within CA265_RS02130 at 534.622 kb on - strand, within CA265_RS02130 at 534.827 kb on + strand, within CA265_RS02130 at 534.850 kb on + strand, within CA265_RS02130 at 534.851 kb on - strand, within CA265_RS02130 at 534.854 kb on - strand, within CA265_RS02130 at 534.854 kb on - strand, within CA265_RS02130 at 534.930 kb on - strand, within CA265_RS02130 at 534.935 kb on - strand, within CA265_RS02130 at 534.935 kb on - strand, within CA265_RS02130 at 534.966 kb on + strand, within CA265_RS02130 at 534.969 kb on - strand, within CA265_RS02130 at 534.989 kb on + strand, within CA265_RS02130 at 534.990 kb on - strand, within CA265_RS02130 at 535.055 kb on + strand, within CA265_RS02130 at 535.102 kb on - strand, within CA265_RS02130 at 535.102 kb on - strand, within CA265_RS02130 at 535.181 kb on - strand, within CA265_RS02130 at 535.184 kb on + strand, within CA265_RS02130 at 535.199 kb on + strand, within CA265_RS02130 at 535.200 kb on - strand, within CA265_RS02130 at 535.228 kb on - strand at 535.271 kb on - strand at 535.271 kb on - strand at 535.351 kb on + strand at 535.374 kb on + strand at 535.375 kb on - strand at 535.447 kb on - strand at 535.502 kb on - strand at 535.532 kb on - strand at 535.612 kb on + strand at 535.684 kb on - strand at 535.727 kb on - strand at 535.729 kb on + strand at 535.754 kb on - strand at 535.788 kb on + strand at 535.788 kb on + strand at 535.793 kb on - strand at 535.847 kb on - strand at 535.876 kb on - strand at 535.887 kb on - strand at 535.905 kb on + strand at 535.983 kb on + strand, within CA265_RS02135 at 535.984 kb on - strand, within CA265_RS02135 at 536.000 kb on - strand, within CA265_RS02135 at 536.000 kb on - strand, within CA265_RS02135 at 536.013 kb on + strand, within CA265_RS02135 at 536.056 kb on + strand, within CA265_RS02135 at 536.101 kb on + strand, within CA265_RS02135 at 536.122 kb on + strand, within CA265_RS02135 at 536.123 kb on - strand, within CA265_RS02135 at 536.153 kb on - strand, within CA265_RS02135 at 536.177 kb on - strand, within CA265_RS02135 at 536.180 kb on - strand, within CA265_RS02135 at 536.245 kb on + strand, within CA265_RS02135 at 536.274 kb on + strand, within CA265_RS02135 at 536.274 kb on + strand, within CA265_RS02135 at 536.274 kb on + strand, within CA265_RS02135 at 536.275 kb on - strand, within CA265_RS02135 at 536.278 kb on + strand, within CA265_RS02135 at 536.329 kb on + strand, within CA265_RS02135 at 536.329 kb on + strand, within CA265_RS02135 at 536.369 kb on - strand, within CA265_RS02135
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 532,076 + CA265_RS02120 0.42 -0.4 532,076 + CA265_RS02120 0.42 -2.5 532,094 - CA265_RS02120 0.44 +0.1 532,169 + CA265_RS02120 0.52 -3.2 532,174 - CA265_RS02120 0.52 -1.3 532,227 - CA265_RS02120 0.57 -0.2 532,228 + CA265_RS02120 0.57 -0.1 532,229 - CA265_RS02120 0.58 +0.1 532,327 + CA265_RS02120 0.67 +2.5 532,327 + CA265_RS02120 0.67 -0.9 532,357 - CA265_RS02120 0.70 -0.9 532,357 - CA265_RS02120 0.70 -1.1 532,385 + CA265_RS02120 0.73 -0.9 532,424 + CA265_RS02120 0.77 -1.3 532,513 - CA265_RS02120 0.86 -0.9 532,526 - CA265_RS02120 0.87 -0.6 532,558 - -1.0 532,615 - -0.9 532,622 + -0.7 532,622 + -1.0 532,623 - -0.5 532,623 - -0.4 532,649 - +0.0 532,649 - +0.7 532,658 + -0.3 532,718 + CA265_RS02125 0.17 +0.2 532,819 + CA265_RS02125 0.45 -0.6 532,831 - CA265_RS02125 0.48 -0.3 532,846 + CA265_RS02125 0.53 -0.3 532,847 - CA265_RS02125 0.53 -0.9 532,994 - +1.7 532,996 + +0.3 533,001 + +0.7 533,019 + +0.1 533,050 - +0.5 533,104 - +0.7 533,128 - -0.9 533,131 - -0.9 533,136 - -0.1 533,246 - -0.1 533,253 + -0.5 533,253 + +0.9 533,254 - +0.4 533,254 - -0.4 533,256 + +0.0 533,257 - -1.5 533,324 - CA265_RS02130 0.12 -0.5 533,387 - CA265_RS02130 0.15 -0.0 533,392 - CA265_RS02130 0.15 +0.6 533,431 + CA265_RS02130 0.17 -0.3 533,431 + CA265_RS02130 0.17 +0.2 533,569 - CA265_RS02130 0.22 -0.3 533,651 + CA265_RS02130 0.26 +0.0 533,652 - CA265_RS02130 0.26 +0.8 533,733 + CA265_RS02130 0.29 -0.1 533,741 + CA265_RS02130 0.30 -0.3 533,784 + CA265_RS02130 0.31 +0.3 533,784 + CA265_RS02130 0.31 -0.0 533,828 + CA265_RS02130 0.33 -0.4 533,847 + CA265_RS02130 0.34 -1.2 533,853 - CA265_RS02130 0.34 -0.3 533,853 - CA265_RS02130 0.34 -0.1 533,856 - CA265_RS02130 0.34 +0.6 533,919 + CA265_RS02130 0.37 -0.1 533,988 + CA265_RS02130 0.40 -0.0 534,051 + CA265_RS02130 0.42 -0.9 534,052 - CA265_RS02130 0.42 -0.0 534,055 + CA265_RS02130 0.42 -0.1 534,055 + CA265_RS02130 0.42 +0.1 534,056 - CA265_RS02130 0.43 +0.5 534,148 + -0.1 534,208 + CA265_RS02130 0.49 +0.3 534,247 + CA265_RS02130 0.50 -0.5 534,248 - CA265_RS02130 0.50 -0.0 534,270 - CA265_RS02130 0.51 -0.1 534,272 + CA265_RS02130 0.51 -0.3 534,273 - CA265_RS02130 0.51 -0.1 534,284 - CA265_RS02130 0.52 -0.4 534,305 - CA265_RS02130 0.53 +0.7 534,345 + CA265_RS02130 0.54 -0.9 534,346 - CA265_RS02130 0.54 +0.7 534,346 - CA265_RS02130 0.54 +0.8 534,351 + CA265_RS02130 0.55 -2.0 534,354 - CA265_RS02130 0.55 -0.4 534,354 - CA265_RS02130 0.55 -0.5 534,494 + CA265_RS02130 0.61 -0.5 534,498 + CA265_RS02130 0.61 -0.5 534,499 - CA265_RS02130 0.61 +0.4 534,499 - CA265_RS02130 0.61 -0.4 534,601 - CA265_RS02130 0.65 -1.8 534,622 - CA265_RS02130 0.66 -1.2 534,827 + CA265_RS02130 0.74 +0.9 534,850 + CA265_RS02130 0.75 +0.3 534,851 - CA265_RS02130 0.75 -0.9 534,854 - CA265_RS02130 0.75 +0.2 534,854 - CA265_RS02130 0.75 -0.3 534,930 - CA265_RS02130 0.78 -0.0 534,935 - CA265_RS02130 0.79 +0.9 534,935 - CA265_RS02130 0.79 +0.5 534,966 + CA265_RS02130 0.80 +1.0 534,969 - CA265_RS02130 0.80 -0.5 534,989 + CA265_RS02130 0.81 -0.7 534,990 - CA265_RS02130 0.81 +0.6 535,055 + CA265_RS02130 0.84 -0.1 535,102 - CA265_RS02130 0.86 -0.5 535,102 - CA265_RS02130 0.86 -0.9 535,181 - CA265_RS02130 0.89 -0.4 535,184 + CA265_RS02130 0.89 -0.4 535,199 + CA265_RS02130 0.90 +0.2 535,200 - CA265_RS02130 0.90 -0.1 535,228 - -0.2 535,271 - -0.9 535,271 - +0.4 535,351 + +2.1 535,374 + +0.5 535,375 - -0.6 535,447 - -2.5 535,502 - +0.5 535,532 - -0.5 535,612 + -0.3 535,684 - +1.8 535,727 - +0.1 535,729 + +1.2 535,754 - -0.7 535,788 + +0.1 535,788 + -0.0 535,793 - -0.4 535,847 - +0.5 535,876 - +0.3 535,887 - -0.2 535,905 + -0.3 535,983 + CA265_RS02135 0.12 -1.2 535,984 - CA265_RS02135 0.12 -0.1 536,000 - CA265_RS02135 0.12 +0.1 536,000 - CA265_RS02135 0.12 -0.2 536,013 + CA265_RS02135 0.13 +0.2 536,056 + CA265_RS02135 0.14 +0.7 536,101 + CA265_RS02135 0.16 -0.3 536,122 + CA265_RS02135 0.16 -0.3 536,123 - CA265_RS02135 0.16 -0.6 536,153 - CA265_RS02135 0.17 +0.0 536,177 - CA265_RS02135 0.18 +0.8 536,180 - CA265_RS02135 0.18 -3.2 536,245 + CA265_RS02135 0.21 -1.4 536,274 + CA265_RS02135 0.22 -1.4 536,274 + CA265_RS02135 0.22 -0.3 536,274 + CA265_RS02135 0.22 -0.2 536,275 - CA265_RS02135 0.22 -0.5 536,278 + CA265_RS02135 0.22 -0.1 536,329 + CA265_RS02135 0.24 -0.1 536,329 + CA265_RS02135 0.24 -0.8 536,369 - CA265_RS02135 0.25 +0.1
Or see this region's nucleotide sequence