Experiment: R2A with Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00350 and CA265_RS00355 are separated by 76 nucleotides CA265_RS00355 and CA265_RS00360 are separated by 206 nucleotides CA265_RS00360 and CA265_RS00365 are separated by 12 nucleotides
CA265_RS00350: CA265_RS00350 - FAD-dependent oxidoreductase, at 80,295 to 81,836
_RS00350
CA265_RS00355: CA265_RS00355 - AraC family transcriptional regulator, at 81,913 to 82,728
_RS00355
CA265_RS00360: CA265_RS00360 - hypothetical protein, at 82,935 to 83,264
_RS00360
CA265_RS00365: CA265_RS00365 - two-component sensor histidine kinase, at 83,277 to 84,632
_RS00365
Position (kb)
81
82
83 Strain fitness (log2 ratio)
-1
0
1 at 80.991 kb on - strand, within CA265_RS00350 at 81.127 kb on + strand, within CA265_RS00350 at 81.127 kb on + strand, within CA265_RS00350 at 81.162 kb on - strand, within CA265_RS00350 at 81.186 kb on + strand, within CA265_RS00350 at 81.408 kb on + strand, within CA265_RS00350 at 81.408 kb on + strand, within CA265_RS00350 at 81.438 kb on + strand, within CA265_RS00350 at 81.438 kb on + strand, within CA265_RS00350 at 81.452 kb on + strand, within CA265_RS00350 at 81.452 kb on + strand, within CA265_RS00350 at 81.453 kb on - strand, within CA265_RS00350 at 81.470 kb on - strand at 81.486 kb on - strand, within CA265_RS00350 at 81.591 kb on - strand, within CA265_RS00350 at 81.617 kb on - strand, within CA265_RS00350 at 81.673 kb on - strand, within CA265_RS00350 at 81.780 kb on - strand at 81.780 kb on - strand at 81.816 kb on - strand at 81.828 kb on - strand at 81.836 kb on + strand at 81.837 kb on - strand at 81.841 kb on + strand at 81.848 kb on - strand at 81.878 kb on + strand at 81.886 kb on + strand at 81.887 kb on - strand at 81.933 kb on + strand at 81.933 kb on + strand at 81.934 kb on - strand at 81.947 kb on - strand at 82.001 kb on + strand, within CA265_RS00355 at 82.001 kb on + strand, within CA265_RS00355 at 82.002 kb on - strand, within CA265_RS00355 at 82.179 kb on + strand, within CA265_RS00355 at 82.405 kb on + strand, within CA265_RS00355 at 82.405 kb on + strand, within CA265_RS00355 at 82.406 kb on - strand, within CA265_RS00355 at 82.519 kb on + strand, within CA265_RS00355 at 82.530 kb on + strand, within CA265_RS00355 at 82.621 kb on + strand, within CA265_RS00355 at 82.621 kb on + strand, within CA265_RS00355 at 82.744 kb on - strand at 82.760 kb on + strand at 82.761 kb on - strand at 82.761 kb on - strand at 82.770 kb on - strand at 82.833 kb on - strand at 82.857 kb on + strand at 82.857 kb on + strand at 82.858 kb on - strand at 82.918 kb on + strand at 83.060 kb on + strand, within CA265_RS00360 at 83.085 kb on + strand, within CA265_RS00360 at 83.122 kb on - strand, within CA265_RS00360 at 83.125 kb on - strand, within CA265_RS00360 at 83.132 kb on + strand, within CA265_RS00360 at 83.133 kb on - strand, within CA265_RS00360 at 83.157 kb on - strand, within CA265_RS00360 at 83.224 kb on + strand, within CA265_RS00360 at 83.343 kb on - strand at 83.390 kb on + strand at 83.395 kb on + strand at 83.427 kb on + strand, within CA265_RS00365 at 83.478 kb on + strand, within CA265_RS00365 at 83.479 kb on - strand, within CA265_RS00365 at 83.479 kb on - strand at 83.489 kb on + strand, within CA265_RS00365 at 83.489 kb on + strand, within CA265_RS00365 at 83.523 kb on - strand, within CA265_RS00365 at 83.568 kb on + strand, within CA265_RS00365 at 83.569 kb on - strand, within CA265_RS00365 at 83.632 kb on - strand, within CA265_RS00365 at 83.667 kb on - strand, within CA265_RS00365 at 83.712 kb on - strand, within CA265_RS00365 at 83.712 kb on - strand, within CA265_RS00365
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.05 mg/ml remove 80,991 - CA265_RS00350 0.45 +0.2 81,127 + CA265_RS00350 0.54 -0.5 81,127 + CA265_RS00350 0.54 +0.6 81,162 - CA265_RS00350 0.56 +0.9 81,186 + CA265_RS00350 0.58 -1.3 81,408 + CA265_RS00350 0.72 -0.8 81,408 + CA265_RS00350 0.72 +1.1 81,438 + CA265_RS00350 0.74 +0.4 81,438 + CA265_RS00350 0.74 +0.8 81,452 + CA265_RS00350 0.75 -0.4 81,452 + CA265_RS00350 0.75 -0.2 81,453 - CA265_RS00350 0.75 -1.0 81,470 - -1.2 81,486 - CA265_RS00350 0.77 +0.6 81,591 - CA265_RS00350 0.84 -0.2 81,617 - CA265_RS00350 0.86 -0.3 81,673 - CA265_RS00350 0.89 +1.6 81,780 - -0.7 81,780 - +0.5 81,816 - -1.4 81,828 - -0.2 81,836 + +0.3 81,837 - -0.3 81,841 + +0.6 81,848 - +1.6 81,878 + -1.0 81,886 + +0.3 81,887 - -0.8 81,933 + -1.1 81,933 + +0.6 81,934 - +1.6 81,947 - -0.7 82,001 + CA265_RS00355 0.11 -0.7 82,001 + CA265_RS00355 0.11 -1.3 82,002 - CA265_RS00355 0.11 +0.1 82,179 + CA265_RS00355 0.33 +0.8 82,405 + CA265_RS00355 0.60 -0.2 82,405 + CA265_RS00355 0.60 -0.4 82,406 - CA265_RS00355 0.60 -0.6 82,519 + CA265_RS00355 0.74 -0.1 82,530 + CA265_RS00355 0.76 +0.1 82,621 + CA265_RS00355 0.87 -0.5 82,621 + CA265_RS00355 0.87 -0.6 82,744 - -1.0 82,760 + +0.2 82,761 - -0.2 82,761 - -0.3 82,770 - -0.3 82,833 - -0.2 82,857 + -0.0 82,857 + +0.0 82,858 - -1.6 82,918 + +0.8 83,060 + CA265_RS00360 0.38 +0.1 83,085 + CA265_RS00360 0.45 +0.9 83,122 - CA265_RS00360 0.57 +0.0 83,125 - CA265_RS00360 0.58 -0.0 83,132 + CA265_RS00360 0.60 +0.1 83,133 - CA265_RS00360 0.60 +0.8 83,157 - CA265_RS00360 0.67 +0.6 83,224 + CA265_RS00360 0.88 +1.1 83,343 - +0.4 83,390 + -0.1 83,395 + -0.4 83,427 + CA265_RS00365 0.11 -1.2 83,478 + CA265_RS00365 0.15 +0.1 83,479 - CA265_RS00365 0.15 +0.2 83,479 - +1.0 83,489 + CA265_RS00365 0.16 +0.0 83,489 + CA265_RS00365 0.16 -1.0 83,523 - CA265_RS00365 0.18 +0.4 83,568 + CA265_RS00365 0.21 -0.9 83,569 - CA265_RS00365 0.22 +0.6 83,632 - CA265_RS00365 0.26 +0.9 83,667 - CA265_RS00365 0.29 -0.8 83,712 - CA265_RS00365 0.32 +0.1 83,712 - CA265_RS00365 0.32 -0.7
Or see this region's nucleotide sequence