Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23455 and EX31_RS23460 are separated by 723 nucleotides EX31_RS23460 and EX31_RS23465 are separated by 14 nucleotides
EX31_RS23455: EX31_RS23455 - succinate--CoA ligase subunit alpha, at 459,718 to 460,590
_RS23455
EX31_RS23460: EX31_RS23460 - cytochrome ubiquinol oxidase subunit I, at 461,314 to 462,882
_RS23460
EX31_RS23465: EX31_RS23465 - cytochrome d ubiquinol oxidase subunit II, at 462,897 to 464,036
_RS23465
Position (kb) 461
462
463 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 460.378 kb on + strand, within EX31_RS23455 at 460.378 kb on + strand, within EX31_RS23455 at 460.414 kb on + strand, within EX31_RS23455 at 460.414 kb on + strand, within EX31_RS23455 at 460.449 kb on + strand, within EX31_RS23455 at 460.449 kb on + strand, within EX31_RS23455 at 460.592 kb on + strand at 460.592 kb on + strand at 460.592 kb on + strand at 460.592 kb on + strand at 460.592 kb on + strand at 460.593 kb on - strand at 460.593 kb on - strand at 460.595 kb on + strand at 460.618 kb on + strand at 460.641 kb on - strand at 460.641 kb on - strand at 460.644 kb on + strand at 460.694 kb on + strand at 460.728 kb on - strand at 460.729 kb on + strand at 460.730 kb on - strand at 460.733 kb on + strand at 460.733 kb on + strand at 460.734 kb on - strand at 460.737 kb on - strand at 460.779 kb on + strand at 460.780 kb on - strand at 460.821 kb on - strand at 460.821 kb on - strand at 460.920 kb on - strand at 460.926 kb on - strand at 460.937 kb on + strand at 460.938 kb on - strand at 460.943 kb on + strand at 460.943 kb on + strand at 461.000 kb on + strand at 461.000 kb on + strand at 461.001 kb on - strand at 461.001 kb on - strand at 461.001 kb on - strand at 461.034 kb on + strand at 461.038 kb on + strand at 461.039 kb on - strand at 461.059 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.065 kb on - strand at 461.093 kb on - strand at 461.188 kb on + strand at 461.188 kb on + strand at 461.231 kb on + strand at 461.237 kb on + strand at 461.238 kb on - strand at 461.238 kb on - strand at 461.238 kb on - strand at 461.366 kb on - strand at 461.366 kb on - strand at 461.366 kb on - strand at 461.375 kb on + strand at 461.431 kb on + strand at 461.432 kb on - strand at 461.455 kb on + strand at 461.463 kb on + strand at 461.463 kb on + strand at 461.463 kb on + strand at 461.463 kb on + strand at 461.464 kb on - strand at 461.496 kb on + strand, within EX31_RS23460 at 461.497 kb on - strand, within EX31_RS23460 at 461.560 kb on + strand, within EX31_RS23460 at 461.560 kb on + strand, within EX31_RS23460 at 461.561 kb on - strand, within EX31_RS23460 at 461.572 kb on + strand, within EX31_RS23460 at 461.572 kb on + strand, within EX31_RS23460 at 461.572 kb on + strand, within EX31_RS23460 at 461.573 kb on - strand, within EX31_RS23460 at 461.573 kb on - strand, within EX31_RS23460 at 461.573 kb on - strand, within EX31_RS23460 at 461.584 kb on - strand, within EX31_RS23460 at 461.642 kb on + strand, within EX31_RS23460 at 461.691 kb on + strand, within EX31_RS23460 at 461.691 kb on + strand, within EX31_RS23460 at 461.691 kb on + strand, within EX31_RS23460 at 461.691 kb on + strand, within EX31_RS23460 at 461.691 kb on + strand, within EX31_RS23460 at 461.691 kb on + strand, within EX31_RS23460 at 461.808 kb on + strand, within EX31_RS23460 at 461.860 kb on - strand, within EX31_RS23460 at 461.871 kb on + strand, within EX31_RS23460 at 461.887 kb on + strand, within EX31_RS23460 at 461.917 kb on - strand, within EX31_RS23460 at 461.926 kb on + strand, within EX31_RS23460 at 461.926 kb on + strand, within EX31_RS23460 at 461.926 kb on + strand, within EX31_RS23460 at 461.926 kb on + strand, within EX31_RS23460 at 461.926 kb on + strand, within EX31_RS23460 at 461.929 kb on + strand, within EX31_RS23460 at 461.929 kb on + strand, within EX31_RS23460 at 461.930 kb on - strand, within EX31_RS23460 at 461.936 kb on + strand, within EX31_RS23460 at 462.056 kb on + strand, within EX31_RS23460 at 462.164 kb on - strand, within EX31_RS23460 at 462.164 kb on - strand, within EX31_RS23460 at 462.181 kb on + strand, within EX31_RS23460 at 462.217 kb on - strand, within EX31_RS23460 at 462.287 kb on - strand, within EX31_RS23460 at 462.287 kb on - strand, within EX31_RS23460 at 462.318 kb on + strand, within EX31_RS23460 at 462.371 kb on - strand, within EX31_RS23460 at 462.375 kb on + strand, within EX31_RS23460 at 462.376 kb on - strand, within EX31_RS23460 at 462.376 kb on - strand, within EX31_RS23460 at 462.376 kb on - strand, within EX31_RS23460 at 462.472 kb on + strand, within EX31_RS23460 at 462.473 kb on - strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.541 kb on - strand, within EX31_RS23460 at 462.578 kb on + strand, within EX31_RS23460 at 462.579 kb on - strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.757 kb on + strand at 462.799 kb on + strand at 462.800 kb on - strand at 462.839 kb on - strand at 462.883 kb on + strand at 462.883 kb on + strand at 462.884 kb on - strand at 462.896 kb on + strand at 462.896 kb on + strand at 462.896 kb on + strand at 462.897 kb on - strand at 462.897 kb on - strand at 462.906 kb on + strand at 462.991 kb on + strand at 463.091 kb on + strand, within EX31_RS23465 at 463.132 kb on - strand, within EX31_RS23465 at 463.155 kb on + strand, within EX31_RS23465 at 463.155 kb on + strand, within EX31_RS23465 at 463.156 kb on - strand, within EX31_RS23465 at 463.430 kb on + strand, within EX31_RS23465 at 463.451 kb on + strand, within EX31_RS23465 at 463.451 kb on + strand, within EX31_RS23465 at 463.469 kb on + strand, within EX31_RS23465 at 463.470 kb on - strand, within EX31_RS23465 at 463.708 kb on + strand, within EX31_RS23465 at 463.754 kb on + strand, within EX31_RS23465 at 463.754 kb on + strand, within EX31_RS23465 at 463.778 kb on + strand, within EX31_RS23465 at 463.823 kb on + strand, within EX31_RS23465 at 463.823 kb on + strand, within EX31_RS23465 at 463.823 kb on + strand, within EX31_RS23465 at 463.823 kb on + strand, within EX31_RS23465 at 463.823 kb on + strand, within EX31_RS23465 at 463.824 kb on - strand, within EX31_RS23465 at 463.824 kb on - strand, within EX31_RS23465 at 463.847 kb on + strand, within EX31_RS23465 at 463.848 kb on - strand, within EX31_RS23465 at 463.848 kb on - strand, within EX31_RS23465
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 460,378 + EX31_RS23455 0.76 -1.3 460,378 + EX31_RS23455 0.76 -1.3 460,414 + EX31_RS23455 0.80 -1.1 460,414 + EX31_RS23455 0.80 +1.6 460,449 + EX31_RS23455 0.84 -2.3 460,449 + EX31_RS23455 0.84 +0.6 460,592 + -1.0 460,592 + +0.9 460,592 + +0.1 460,592 + +0.8 460,592 + +0.1 460,593 - -1.4 460,593 - -1.7 460,595 + -0.2 460,618 + +0.7 460,641 - -3.1 460,641 - +0.2 460,644 + +1.9 460,694 + -0.4 460,728 - +0.3 460,729 + +0.2 460,730 - +1.9 460,733 + -0.0 460,733 + -1.1 460,734 - +1.1 460,737 - +2.2 460,779 + -1.9 460,780 - +0.1 460,821 - +1.9 460,821 - +0.2 460,920 - -0.5 460,926 - +0.7 460,937 + +0.4 460,938 - +1.3 460,943 + +1.9 460,943 + +1.3 461,000 + +2.4 461,000 + +1.7 461,001 - +0.2 461,001 - +1.7 461,001 - +0.2 461,034 + -1.9 461,038 + +0.0 461,039 - +0.9 461,059 + +1.7 461,064 + -0.5 461,064 + -0.4 461,064 + -2.2 461,064 + -0.1 461,064 + +0.5 461,064 + +0.9 461,064 + -1.7 461,065 - +0.7 461,093 - +1.4 461,188 + +0.9 461,188 + -1.1 461,231 + -0.5 461,237 + -1.9 461,238 - +0.2 461,238 - +0.5 461,238 - +0.8 461,366 - -0.7 461,366 - -1.5 461,366 - +1.5 461,375 + -1.5 461,431 + +0.5 461,432 - -2.1 461,455 + -0.9 461,463 + +0.9 461,463 + -0.1 461,463 + -2.1 461,463 + +0.4 461,464 - -0.2 461,496 + EX31_RS23460 0.12 +0.9 461,497 - EX31_RS23460 0.12 -0.4 461,560 + EX31_RS23460 0.16 -1.8 461,560 + EX31_RS23460 0.16 -0.4 461,561 - EX31_RS23460 0.16 +1.9 461,572 + EX31_RS23460 0.16 +0.6 461,572 + EX31_RS23460 0.16 -0.6 461,572 + EX31_RS23460 0.16 -2.7 461,573 - EX31_RS23460 0.17 -1.5 461,573 - EX31_RS23460 0.17 +0.8 461,573 - EX31_RS23460 0.17 -1.7 461,584 - EX31_RS23460 0.17 +0.9 461,642 + EX31_RS23460 0.21 +0.5 461,691 + EX31_RS23460 0.24 -0.6 461,691 + EX31_RS23460 0.24 -0.9 461,691 + EX31_RS23460 0.24 +0.9 461,691 + EX31_RS23460 0.24 +0.6 461,691 + EX31_RS23460 0.24 -0.9 461,691 + EX31_RS23460 0.24 +0.6 461,808 + EX31_RS23460 0.31 +0.3 461,860 - EX31_RS23460 0.35 -2.1 461,871 + EX31_RS23460 0.36 -0.2 461,887 + EX31_RS23460 0.37 -0.3 461,917 - EX31_RS23460 0.38 -0.9 461,926 + EX31_RS23460 0.39 +0.9 461,926 + EX31_RS23460 0.39 +1.4 461,926 + EX31_RS23460 0.39 -1.9 461,926 + EX31_RS23460 0.39 +0.8 461,926 + EX31_RS23460 0.39 -1.1 461,929 + EX31_RS23460 0.39 +1.9 461,929 + EX31_RS23460 0.39 +0.3 461,930 - EX31_RS23460 0.39 -1.1 461,936 + EX31_RS23460 0.40 -0.5 462,056 + EX31_RS23460 0.47 -1.3 462,164 - EX31_RS23460 0.54 +1.5 462,164 - EX31_RS23460 0.54 -0.7 462,181 + EX31_RS23460 0.55 -0.7 462,217 - EX31_RS23460 0.58 -0.4 462,287 - EX31_RS23460 0.62 +0.7 462,287 - EX31_RS23460 0.62 +0.3 462,318 + EX31_RS23460 0.64 -0.2 462,371 - EX31_RS23460 0.67 -1.7 462,375 + EX31_RS23460 0.68 -0.7 462,376 - EX31_RS23460 0.68 -2.0 462,376 - EX31_RS23460 0.68 -0.3 462,376 - EX31_RS23460 0.68 -1.5 462,472 + EX31_RS23460 0.74 -0.9 462,473 - EX31_RS23460 0.74 +0.1 462,538 + EX31_RS23460 0.78 -1.8 462,538 + EX31_RS23460 0.78 -0.8 462,538 + EX31_RS23460 0.78 -1.8 462,538 + EX31_RS23460 0.78 -0.1 462,538 + EX31_RS23460 0.78 +0.0 462,538 + EX31_RS23460 0.78 +1.4 462,538 + EX31_RS23460 0.78 -0.1 462,541 - EX31_RS23460 0.78 -0.7 462,578 + EX31_RS23460 0.81 -2.2 462,579 - EX31_RS23460 0.81 -1.3 462,725 + EX31_RS23460 0.90 +0.7 462,725 + EX31_RS23460 0.90 -1.4 462,725 + EX31_RS23460 0.90 -0.9 462,725 + EX31_RS23460 0.90 +0.3 462,725 + EX31_RS23460 0.90 -1.1 462,725 + EX31_RS23460 0.90 +0.2 462,725 + EX31_RS23460 0.90 -0.5 462,725 + EX31_RS23460 0.90 -1.7 462,725 + EX31_RS23460 0.90 -2.0 462,757 + +0.9 462,799 + -1.5 462,800 - +1.6 462,839 - -2.1 462,883 + +0.3 462,883 + +0.1 462,884 - +0.3 462,896 + -1.7 462,896 + -2.6 462,896 + -0.9 462,897 - -1.1 462,897 - -1.5 462,906 + -0.7 462,991 + -1.1 463,091 + EX31_RS23465 0.17 +0.9 463,132 - EX31_RS23465 0.21 -1.1 463,155 + EX31_RS23465 0.23 +0.9 463,155 + EX31_RS23465 0.23 +1.3 463,156 - EX31_RS23465 0.23 +0.9 463,430 + EX31_RS23465 0.47 -2.2 463,451 + EX31_RS23465 0.49 -0.9 463,451 + EX31_RS23465 0.49 -0.2 463,469 + EX31_RS23465 0.50 -0.3 463,470 - EX31_RS23465 0.50 -1.1 463,708 + EX31_RS23465 0.71 -0.4 463,754 + EX31_RS23465 0.75 -0.5 463,754 + EX31_RS23465 0.75 -0.2 463,778 + EX31_RS23465 0.77 +1.3 463,823 + EX31_RS23465 0.81 +0.4 463,823 + EX31_RS23465 0.81 +0.3 463,823 + EX31_RS23465 0.81 -0.4 463,823 + EX31_RS23465 0.81 -0.5 463,823 + EX31_RS23465 0.81 +1.9 463,824 - EX31_RS23465 0.81 +0.5 463,824 - EX31_RS23465 0.81 +0.6 463,847 + EX31_RS23465 0.83 -0.7 463,848 - EX31_RS23465 0.83 -0.9 463,848 - EX31_RS23465 0.83 +1.9
Or see this region's nucleotide sequence