Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23450 and EX31_RS23455 overlap by 1 nucleotides EX31_RS23455 and EX31_RS23460 are separated by 723 nucleotides
EX31_RS23450: EX31_RS23450 - ADP-forming succinate--CoA ligase subunit beta, at 458,552 to 459,718
_RS23450
EX31_RS23455: EX31_RS23455 - succinate--CoA ligase subunit alpha, at 459,718 to 460,590
_RS23455
EX31_RS23460: EX31_RS23460 - cytochrome ubiquinol oxidase subunit I, at 461,314 to 462,882
_RS23460
Position (kb) 459
460
461 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 458.802 kb on - strand, within EX31_RS23450 at 458.929 kb on + strand, within EX31_RS23450 at 458.929 kb on + strand, within EX31_RS23450 at 458.929 kb on + strand, within EX31_RS23450 at 458.930 kb on - strand, within EX31_RS23450 at 459.305 kb on + strand, within EX31_RS23450 at 459.305 kb on + strand, within EX31_RS23450 at 459.306 kb on - strand, within EX31_RS23450 at 459.306 kb on - strand, within EX31_RS23450 at 459.604 kb on + strand at 459.750 kb on + strand at 459.750 kb on + strand at 459.899 kb on + strand, within EX31_RS23455 at 459.906 kb on + strand, within EX31_RS23455 at 459.932 kb on - strand, within EX31_RS23455 at 460.002 kb on + strand, within EX31_RS23455 at 460.098 kb on + strand, within EX31_RS23455 at 460.192 kb on + strand, within EX31_RS23455 at 460.193 kb on - strand, within EX31_RS23455 at 460.236 kb on + strand, within EX31_RS23455 at 460.275 kb on + strand, within EX31_RS23455 at 460.378 kb on + strand, within EX31_RS23455 at 460.378 kb on + strand, within EX31_RS23455 at 460.414 kb on + strand, within EX31_RS23455 at 460.414 kb on + strand, within EX31_RS23455 at 460.449 kb on + strand, within EX31_RS23455 at 460.449 kb on + strand, within EX31_RS23455 at 460.592 kb on + strand at 460.592 kb on + strand at 460.592 kb on + strand at 460.592 kb on + strand at 460.592 kb on + strand at 460.593 kb on - strand at 460.593 kb on - strand at 460.595 kb on + strand at 460.618 kb on + strand at 460.641 kb on - strand at 460.641 kb on - strand at 460.644 kb on + strand at 460.694 kb on + strand at 460.728 kb on - strand at 460.729 kb on + strand at 460.730 kb on - strand at 460.733 kb on + strand at 460.733 kb on + strand at 460.734 kb on - strand at 460.737 kb on - strand at 460.779 kb on + strand at 460.780 kb on - strand at 460.821 kb on - strand at 460.821 kb on - strand at 460.920 kb on - strand at 460.926 kb on - strand at 460.937 kb on + strand at 460.938 kb on - strand at 460.943 kb on + strand at 460.943 kb on + strand at 461.000 kb on + strand at 461.000 kb on + strand at 461.001 kb on - strand at 461.001 kb on - strand at 461.001 kb on - strand at 461.034 kb on + strand at 461.038 kb on + strand at 461.039 kb on - strand at 461.059 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.064 kb on + strand at 461.065 kb on - strand at 461.093 kb on - strand at 461.188 kb on + strand at 461.188 kb on + strand at 461.231 kb on + strand at 461.237 kb on + strand at 461.238 kb on - strand at 461.238 kb on - strand at 461.238 kb on - strand at 461.366 kb on - strand at 461.366 kb on - strand at 461.366 kb on - strand at 461.375 kb on + strand at 461.431 kb on + strand at 461.432 kb on - strand at 461.455 kb on + strand at 461.463 kb on + strand at 461.463 kb on + strand at 461.463 kb on + strand at 461.463 kb on + strand at 461.464 kb on - strand at 461.496 kb on + strand, within EX31_RS23460 at 461.497 kb on - strand, within EX31_RS23460 at 461.560 kb on + strand, within EX31_RS23460 at 461.560 kb on + strand, within EX31_RS23460 at 461.561 kb on - strand, within EX31_RS23460 at 461.572 kb on + strand, within EX31_RS23460 at 461.572 kb on + strand, within EX31_RS23460 at 461.572 kb on + strand, within EX31_RS23460 at 461.573 kb on - strand, within EX31_RS23460 at 461.573 kb on - strand, within EX31_RS23460 at 461.573 kb on - strand, within EX31_RS23460 at 461.584 kb on - strand, within EX31_RS23460
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 458,802 - EX31_RS23450 0.21 -1.3 458,929 + EX31_RS23450 0.32 -0.9 458,929 + EX31_RS23450 0.32 -0.5 458,929 + EX31_RS23450 0.32 -0.9 458,930 - EX31_RS23450 0.32 +1.4 459,305 + EX31_RS23450 0.65 -1.9 459,305 + EX31_RS23450 0.65 +0.8 459,306 - EX31_RS23450 0.65 +0.3 459,306 - EX31_RS23450 0.65 -1.3 459,604 + -0.7 459,750 + +2.9 459,750 + +1.7 459,899 + EX31_RS23455 0.21 +0.5 459,906 + EX31_RS23455 0.22 +2.9 459,932 - EX31_RS23455 0.25 -0.5 460,002 + EX31_RS23455 0.33 -1.4 460,098 + EX31_RS23455 0.44 +0.3 460,192 + EX31_RS23455 0.54 -1.4 460,193 - EX31_RS23455 0.54 -0.3 460,236 + EX31_RS23455 0.59 -1.4 460,275 + EX31_RS23455 0.64 -0.4 460,378 + EX31_RS23455 0.76 -1.3 460,378 + EX31_RS23455 0.76 -1.3 460,414 + EX31_RS23455 0.80 -1.1 460,414 + EX31_RS23455 0.80 +1.6 460,449 + EX31_RS23455 0.84 -2.3 460,449 + EX31_RS23455 0.84 +0.6 460,592 + -1.0 460,592 + +0.9 460,592 + +0.1 460,592 + +0.8 460,592 + +0.1 460,593 - -1.4 460,593 - -1.7 460,595 + -0.2 460,618 + +0.7 460,641 - -3.1 460,641 - +0.2 460,644 + +1.9 460,694 + -0.4 460,728 - +0.3 460,729 + +0.2 460,730 - +1.9 460,733 + -0.0 460,733 + -1.1 460,734 - +1.1 460,737 - +2.2 460,779 + -1.9 460,780 - +0.1 460,821 - +1.9 460,821 - +0.2 460,920 - -0.5 460,926 - +0.7 460,937 + +0.4 460,938 - +1.3 460,943 + +1.9 460,943 + +1.3 461,000 + +2.4 461,000 + +1.7 461,001 - +0.2 461,001 - +1.7 461,001 - +0.2 461,034 + -1.9 461,038 + +0.0 461,039 - +0.9 461,059 + +1.7 461,064 + -0.5 461,064 + -0.4 461,064 + -2.2 461,064 + -0.1 461,064 + +0.5 461,064 + +0.9 461,064 + -1.7 461,065 - +0.7 461,093 - +1.4 461,188 + +0.9 461,188 + -1.1 461,231 + -0.5 461,237 + -1.9 461,238 - +0.2 461,238 - +0.5 461,238 - +0.8 461,366 - -0.7 461,366 - -1.5 461,366 - +1.5 461,375 + -1.5 461,431 + +0.5 461,432 - -2.1 461,455 + -0.9 461,463 + +0.9 461,463 + -0.1 461,463 + -2.1 461,463 + +0.4 461,464 - -0.2 461,496 + EX31_RS23460 0.12 +0.9 461,497 - EX31_RS23460 0.12 -0.4 461,560 + EX31_RS23460 0.16 -1.8 461,560 + EX31_RS23460 0.16 -0.4 461,561 - EX31_RS23460 0.16 +1.9 461,572 + EX31_RS23460 0.16 +0.6 461,572 + EX31_RS23460 0.16 -0.6 461,572 + EX31_RS23460 0.16 -2.7 461,573 - EX31_RS23460 0.17 -1.5 461,573 - EX31_RS23460 0.17 +0.8 461,573 - EX31_RS23460 0.17 -1.7 461,584 - EX31_RS23460 0.17 +0.9
Or see this region's nucleotide sequence