Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21740 and EX31_RS21745 are separated by 156 nucleotides EX31_RS21745 and EX31_RS21750 overlap by 8 nucleotides EX31_RS21750 and EX31_RS21755 are separated by 76 nucleotides EX31_RS21755 and EX31_RS21760 are separated by 112 nucleotides
EX31_RS21740: EX31_RS21740 - SsrA-binding protein SmpB, at 92,848 to 93,330
_RS21740
EX31_RS21745: EX31_RS21745 - type II toxin-antitoxin system RatA family toxin, at 93,487 to 93,921
_RS21745
EX31_RS21750: EX31_RS21750 - RnfH family protein, at 93,914 to 94,198
_RS21750
EX31_RS21755: EX31_RS21755 - outer membrane protein assembly factor BamE, at 94,275 to 94,619
_RS21755
EX31_RS21760: EX31_RS21760 - DNA repair protein RecN, at 94,732 to 96,411
_RS21760
Position (kb) 93
94
95 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 93.062 kb on - strand, within EX31_RS21740 at 93.094 kb on - strand, within EX31_RS21740 at 93.112 kb on - strand, within EX31_RS21740 at 93.168 kb on - strand, within EX31_RS21740 at 93.331 kb on - strand at 93.337 kb on - strand at 93.337 kb on - strand at 93.337 kb on - strand at 93.337 kb on - strand at 93.337 kb on - strand at 93.337 kb on - strand at 93.337 kb on - strand at 93.337 kb on - strand at 93.337 kb on - strand at 93.337 kb on - strand at 93.337 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.341 kb on - strand at 93.344 kb on - strand at 93.344 kb on - strand at 93.344 kb on - strand at 93.344 kb on - strand at 93.344 kb on - strand at 93.344 kb on - strand at 93.346 kb on - strand at 93.346 kb on - strand at 93.351 kb on - strand at 93.351 kb on - strand at 93.351 kb on - strand at 93.444 kb on + strand at 93.445 kb on - strand at 93.445 kb on - strand at 93.445 kb on - strand at 93.451 kb on + strand at 93.452 kb on - strand at 93.454 kb on + strand at 93.454 kb on + strand at 93.454 kb on + strand at 93.455 kb on - strand at 93.460 kb on + strand at 93.467 kb on + strand at 93.910 kb on + strand at 93.910 kb on + strand at 93.910 kb on + strand at 93.910 kb on + strand at 93.910 kb on + strand at 93.910 kb on + strand at 93.910 kb on + strand at 93.911 kb on - strand at 93.911 kb on - strand at 93.911 kb on - strand at 93.911 kb on - strand at 93.911 kb on - strand at 93.911 kb on - strand at 93.911 kb on - strand at 93.941 kb on + strand at 93.963 kb on - strand, within EX31_RS21750 at 93.963 kb on - strand, within EX31_RS21750 at 94.045 kb on + strand, within EX31_RS21750 at 94.063 kb on + strand, within EX31_RS21750 at 94.063 kb on + strand, within EX31_RS21750 at 94.063 kb on + strand, within EX31_RS21750 at 94.064 kb on - strand, within EX31_RS21750 at 94.064 kb on - strand, within EX31_RS21750 at 94.068 kb on + strand, within EX31_RS21750 at 94.068 kb on + strand, within EX31_RS21750 at 94.076 kb on + strand, within EX31_RS21750 at 94.076 kb on + strand, within EX31_RS21750 at 94.076 kb on + strand, within EX31_RS21750 at 94.077 kb on - strand, within EX31_RS21750 at 94.077 kb on - strand, within EX31_RS21750 at 94.077 kb on - strand, within EX31_RS21750 at 94.102 kb on + strand, within EX31_RS21750 at 94.102 kb on + strand, within EX31_RS21750 at 94.171 kb on + strand at 94.171 kb on + strand at 94.173 kb on + strand at 94.173 kb on + strand at 94.173 kb on + strand at 94.174 kb on - strand at 94.174 kb on - strand at 94.196 kb on + strand at 94.276 kb on + strand at 94.295 kb on + strand at 94.295 kb on + strand at 94.295 kb on + strand at 94.295 kb on + strand at 94.295 kb on + strand at 94.296 kb on - strand at 94.296 kb on - strand at 94.296 kb on - strand at 94.296 kb on - strand at 94.296 kb on - strand at 94.323 kb on - strand, within EX31_RS21755 at 94.323 kb on - strand, within EX31_RS21755 at 94.365 kb on - strand, within EX31_RS21755 at 94.396 kb on + strand, within EX31_RS21755 at 94.396 kb on + strand, within EX31_RS21755 at 94.396 kb on + strand, within EX31_RS21755 at 94.396 kb on + strand, within EX31_RS21755 at 94.397 kb on - strand, within EX31_RS21755 at 94.397 kb on - strand, within EX31_RS21755 at 94.397 kb on - strand, within EX31_RS21755 at 94.397 kb on - strand, within EX31_RS21755 at 94.397 kb on - strand, within EX31_RS21755 at 94.397 kb on - strand, within EX31_RS21755 at 94.397 kb on - strand, within EX31_RS21755 at 94.397 kb on - strand, within EX31_RS21755 at 94.397 kb on - strand, within EX31_RS21755 at 94.397 kb on - strand, within EX31_RS21755 at 94.397 kb on - strand, within EX31_RS21755 at 94.400 kb on - strand, within EX31_RS21755 at 94.400 kb on - strand, within EX31_RS21755 at 94.409 kb on + strand, within EX31_RS21755 at 94.429 kb on - strand, within EX31_RS21755 at 94.429 kb on - strand, within EX31_RS21755 at 94.429 kb on - strand, within EX31_RS21755 at 94.450 kb on + strand, within EX31_RS21755 at 94.450 kb on + strand, within EX31_RS21755 at 94.450 kb on + strand, within EX31_RS21755 at 94.450 kb on + strand, within EX31_RS21755 at 94.450 kb on + strand, within EX31_RS21755 at 94.451 kb on - strand, within EX31_RS21755 at 94.451 kb on - strand, within EX31_RS21755 at 94.451 kb on - strand, within EX31_RS21755 at 94.451 kb on - strand, within EX31_RS21755 at 94.451 kb on - strand, within EX31_RS21755 at 94.451 kb on - strand, within EX31_RS21755 at 94.451 kb on - strand, within EX31_RS21755 at 94.451 kb on - strand, within EX31_RS21755 at 94.467 kb on - strand, within EX31_RS21755 at 94.473 kb on - strand, within EX31_RS21755 at 94.510 kb on + strand, within EX31_RS21755 at 94.510 kb on + strand, within EX31_RS21755 at 94.510 kb on + strand, within EX31_RS21755 at 94.510 kb on + strand, within EX31_RS21755 at 94.510 kb on + strand, within EX31_RS21755 at 94.510 kb on + strand, within EX31_RS21755 at 94.510 kb on + strand, within EX31_RS21755 at 94.511 kb on - strand, within EX31_RS21755 at 94.511 kb on - strand, within EX31_RS21755 at 94.511 kb on - strand, within EX31_RS21755 at 94.511 kb on - strand, within EX31_RS21755 at 94.511 kb on - strand, within EX31_RS21755 at 94.511 kb on - strand, within EX31_RS21755 at 94.572 kb on - strand, within EX31_RS21755 at 94.579 kb on - strand, within EX31_RS21755 at 94.579 kb on - strand, within EX31_RS21755 at 94.579 kb on - strand, within EX31_RS21755 at 94.588 kb on - strand at 94.588 kb on - strand at 94.588 kb on - strand at 94.611 kb on - strand at 94.611 kb on - strand at 94.619 kb on + strand at 94.620 kb on - strand at 94.620 kb on - strand at 94.620 kb on - strand at 94.620 kb on - strand at 94.620 kb on - strand at 94.620 kb on - strand at 94.626 kb on + strand at 94.626 kb on + strand at 94.626 kb on + strand at 94.627 kb on - strand at 94.627 kb on - strand at 94.627 kb on - strand at 94.627 kb on - strand at 94.627 kb on - strand at 94.627 kb on - strand at 94.627 kb on - strand at 94.627 kb on - strand at 94.637 kb on - strand at 94.643 kb on + strand at 94.643 kb on + strand at 94.643 kb on + strand at 94.644 kb on - strand at 94.644 kb on - strand at 94.658 kb on - strand at 94.658 kb on - strand at 94.660 kb on + strand at 94.660 kb on + strand at 94.660 kb on + strand at 94.661 kb on - strand at 94.661 kb on - strand at 94.699 kb on + strand at 94.699 kb on + strand at 94.699 kb on + strand at 94.699 kb on + strand at 94.700 kb on - strand at 94.700 kb on - strand at 94.700 kb on - strand at 94.700 kb on - strand at 94.716 kb on - strand at 94.734 kb on - strand at 94.745 kb on - strand at 94.827 kb on + strand at 94.827 kb on + strand at 94.886 kb on + strand at 94.887 kb on - strand at 94.887 kb on - strand at 94.887 kb on - strand at 94.918 kb on + strand, within EX31_RS21760 at 94.918 kb on + strand, within EX31_RS21760 at 94.918 kb on + strand, within EX31_RS21760 at 94.918 kb on + strand, within EX31_RS21760 at 94.919 kb on - strand, within EX31_RS21760 at 94.919 kb on - strand, within EX31_RS21760 at 94.935 kb on + strand, within EX31_RS21760 at 94.935 kb on + strand, within EX31_RS21760 at 94.935 kb on + strand, within EX31_RS21760 at 94.935 kb on + strand, within EX31_RS21760 at 94.935 kb on + strand, within EX31_RS21760 at 94.935 kb on + strand, within EX31_RS21760 at 94.935 kb on + strand, within EX31_RS21760 at 94.935 kb on + strand, within EX31_RS21760 at 94.935 kb on + strand, within EX31_RS21760 at 94.936 kb on - strand, within EX31_RS21760 at 94.936 kb on - strand, within EX31_RS21760 at 94.936 kb on - strand, within EX31_RS21760 at 94.936 kb on - strand, within EX31_RS21760 at 94.936 kb on - strand, within EX31_RS21760 at 94.936 kb on - strand, within EX31_RS21760 at 94.936 kb on - strand, within EX31_RS21760 at 95.001 kb on + strand, within EX31_RS21760 at 95.079 kb on + strand, within EX31_RS21760 at 95.080 kb on - strand, within EX31_RS21760 at 95.178 kb on + strand, within EX31_RS21760 at 95.178 kb on + strand, within EX31_RS21760 at 95.178 kb on + strand, within EX31_RS21760 at 95.179 kb on - strand, within EX31_RS21760 at 95.182 kb on + strand, within EX31_RS21760 at 95.182 kb on + strand, within EX31_RS21760 at 95.182 kb on + strand, within EX31_RS21760 at 95.182 kb on + strand, within EX31_RS21760 at 95.182 kb on + strand, within EX31_RS21760 at 95.183 kb on - strand, within EX31_RS21760 at 95.183 kb on - strand, within EX31_RS21760 at 95.183 kb on - strand, within EX31_RS21760
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 93,062 - EX31_RS21740 0.44 -1.1 93,094 - EX31_RS21740 0.51 +2.1 93,112 - EX31_RS21740 0.55 -0.7 93,168 - EX31_RS21740 0.66 +1.1 93,331 - -1.1 93,337 - +1.5 93,337 - -0.4 93,337 - -0.7 93,337 - -2.3 93,337 - -0.4 93,337 - +0.2 93,337 - +1.3 93,337 - -0.2 93,337 - -0.4 93,337 - +0.9 93,337 - -2.0 93,341 - -0.9 93,341 - +1.9 93,341 - +0.4 93,341 - +0.5 93,341 - -1.5 93,341 - +0.6 93,341 - +0.3 93,341 - +0.9 93,341 - -0.0 93,341 - -0.6 93,341 - +1.2 93,341 - +0.4 93,341 - -0.3 93,344 - +0.4 93,344 - -1.6 93,344 - +0.7 93,344 - +0.1 93,344 - -2.5 93,344 - -0.8 93,346 - -0.7 93,346 - +0.0 93,351 - +0.2 93,351 - +0.9 93,351 - -0.1 93,444 + -0.2 93,445 - -0.2 93,445 - -0.6 93,445 - -0.3 93,451 + +0.4 93,452 - -1.5 93,454 + +0.6 93,454 + +1.4 93,454 + -2.0 93,455 - -0.3 93,460 + +0.2 93,467 + -0.2 93,910 + -0.7 93,910 + -2.2 93,910 + -0.0 93,910 + +0.6 93,910 + -1.6 93,910 + +0.1 93,910 + -1.1 93,911 - +1.1 93,911 - +2.1 93,911 - +2.6 93,911 - -0.6 93,911 - -0.9 93,911 - -1.1 93,911 - +0.3 93,941 + -0.7 93,963 - EX31_RS21750 0.17 +1.5 93,963 - EX31_RS21750 0.17 -2.1 94,045 + EX31_RS21750 0.46 -0.5 94,063 + EX31_RS21750 0.52 -0.7 94,063 + EX31_RS21750 0.52 +0.2 94,063 + EX31_RS21750 0.52 -0.2 94,064 - EX31_RS21750 0.53 +0.5 94,064 - EX31_RS21750 0.53 -1.6 94,068 + EX31_RS21750 0.54 -0.1 94,068 + EX31_RS21750 0.54 -0.7 94,076 + EX31_RS21750 0.57 -0.7 94,076 + EX31_RS21750 0.57 +0.3 94,076 + EX31_RS21750 0.57 -0.1 94,077 - EX31_RS21750 0.57 +1.5 94,077 - EX31_RS21750 0.57 -0.9 94,077 - EX31_RS21750 0.57 -1.3 94,102 + EX31_RS21750 0.66 -1.6 94,102 + EX31_RS21750 0.66 -0.3 94,171 + +1.5 94,171 + +0.8 94,173 + +0.9 94,173 + -0.6 94,173 + -0.5 94,174 - -1.4 94,174 - +0.7 94,196 + -1.1 94,276 + -0.2 94,295 + +1.4 94,295 + +0.1 94,295 + +1.6 94,295 + +0.3 94,295 + +0.1 94,296 - -0.7 94,296 - -0.1 94,296 - +0.5 94,296 - -0.4 94,296 - +0.1 94,323 - EX31_RS21755 0.14 -0.7 94,323 - EX31_RS21755 0.14 +2.1 94,365 - EX31_RS21755 0.26 +0.3 94,396 + EX31_RS21755 0.35 -0.9 94,396 + EX31_RS21755 0.35 +0.1 94,396 + EX31_RS21755 0.35 +0.4 94,396 + EX31_RS21755 0.35 -0.4 94,397 - EX31_RS21755 0.35 +2.3 94,397 - EX31_RS21755 0.35 +1.1 94,397 - EX31_RS21755 0.35 +0.7 94,397 - EX31_RS21755 0.35 -0.2 94,397 - EX31_RS21755 0.35 +0.6 94,397 - EX31_RS21755 0.35 +0.2 94,397 - EX31_RS21755 0.35 +0.9 94,397 - EX31_RS21755 0.35 +0.4 94,397 - EX31_RS21755 0.35 +0.3 94,397 - EX31_RS21755 0.35 +0.6 94,397 - EX31_RS21755 0.35 -1.5 94,400 - EX31_RS21755 0.36 -1.2 94,400 - EX31_RS21755 0.36 -0.7 94,409 + EX31_RS21755 0.39 +0.7 94,429 - EX31_RS21755 0.45 +0.2 94,429 - EX31_RS21755 0.45 -0.4 94,429 - EX31_RS21755 0.45 -0.5 94,450 + EX31_RS21755 0.51 -1.3 94,450 + EX31_RS21755 0.51 -0.8 94,450 + EX31_RS21755 0.51 +0.5 94,450 + EX31_RS21755 0.51 +0.3 94,450 + EX31_RS21755 0.51 +0.4 94,451 - EX31_RS21755 0.51 -1.3 94,451 - EX31_RS21755 0.51 -1.0 94,451 - EX31_RS21755 0.51 -1.1 94,451 - EX31_RS21755 0.51 +1.2 94,451 - EX31_RS21755 0.51 -1.7 94,451 - EX31_RS21755 0.51 -1.0 94,451 - EX31_RS21755 0.51 -0.3 94,451 - EX31_RS21755 0.51 -0.5 94,467 - EX31_RS21755 0.56 -0.5 94,473 - EX31_RS21755 0.57 -0.0 94,510 + EX31_RS21755 0.68 -1.3 94,510 + EX31_RS21755 0.68 +0.2 94,510 + EX31_RS21755 0.68 -0.1 94,510 + EX31_RS21755 0.68 -2.8 94,510 + EX31_RS21755 0.68 -1.3 94,510 + EX31_RS21755 0.68 -1.8 94,510 + EX31_RS21755 0.68 -0.5 94,511 - EX31_RS21755 0.68 -0.4 94,511 - EX31_RS21755 0.68 -0.2 94,511 - EX31_RS21755 0.68 -0.1 94,511 - EX31_RS21755 0.68 +0.2 94,511 - EX31_RS21755 0.68 +0.5 94,511 - EX31_RS21755 0.68 -1.8 94,572 - EX31_RS21755 0.86 -0.2 94,579 - EX31_RS21755 0.88 -0.2 94,579 - EX31_RS21755 0.88 +0.6 94,579 - EX31_RS21755 0.88 -1.7 94,588 - -0.1 94,588 - -0.2 94,588 - +0.3 94,611 - -0.0 94,611 - -0.4 94,619 + -0.9 94,620 - +1.2 94,620 - +0.4 94,620 - -1.7 94,620 - -0.2 94,620 - -0.3 94,620 - +0.7 94,626 + -0.2 94,626 + -0.6 94,626 + +0.2 94,627 - +0.3 94,627 - +0.7 94,627 - -0.3 94,627 - +0.3 94,627 - -1.2 94,627 - -0.6 94,627 - -0.1 94,627 - +1.3 94,637 - +2.0 94,643 + +2.4 94,643 + -1.0 94,643 + -1.4 94,644 - +0.4 94,644 - +1.3 94,658 - +0.3 94,658 - -0.3 94,660 + +0.6 94,660 + +0.4 94,660 + +2.9 94,661 - +1.0 94,661 - -0.3 94,699 + +1.1 94,699 + -0.8 94,699 + -0.7 94,699 + +0.8 94,700 - +0.8 94,700 - -0.6 94,700 - +0.6 94,700 - +0.5 94,716 - +0.2 94,734 - +0.0 94,745 - +0.6 94,827 + +0.7 94,827 + +0.8 94,886 + -2.5 94,887 - +0.5 94,887 - -0.6 94,887 - -2.1 94,918 + EX31_RS21760 0.11 +0.6 94,918 + EX31_RS21760 0.11 -0.8 94,918 + EX31_RS21760 0.11 +0.1 94,918 + EX31_RS21760 0.11 -0.8 94,919 - EX31_RS21760 0.11 -0.1 94,919 - EX31_RS21760 0.11 -0.6 94,935 + EX31_RS21760 0.12 +0.7 94,935 + EX31_RS21760 0.12 +0.3 94,935 + EX31_RS21760 0.12 -1.2 94,935 + EX31_RS21760 0.12 +0.1 94,935 + EX31_RS21760 0.12 +1.8 94,935 + EX31_RS21760 0.12 -0.5 94,935 + EX31_RS21760 0.12 +0.1 94,935 + EX31_RS21760 0.12 +0.3 94,935 + EX31_RS21760 0.12 +1.3 94,936 - EX31_RS21760 0.12 -0.4 94,936 - EX31_RS21760 0.12 +0.4 94,936 - EX31_RS21760 0.12 +0.8 94,936 - EX31_RS21760 0.12 +1.2 94,936 - EX31_RS21760 0.12 -0.3 94,936 - EX31_RS21760 0.12 +1.5 94,936 - EX31_RS21760 0.12 -0.9 95,001 + EX31_RS21760 0.16 +0.8 95,079 + EX31_RS21760 0.21 +0.6 95,080 - EX31_RS21760 0.21 -2.5 95,178 + EX31_RS21760 0.27 +2.1 95,178 + EX31_RS21760 0.27 -0.5 95,178 + EX31_RS21760 0.27 -1.0 95,179 - EX31_RS21760 0.27 +0.6 95,182 + EX31_RS21760 0.27 -0.2 95,182 + EX31_RS21760 0.27 -0.0 95,182 + EX31_RS21760 0.27 +0.8 95,182 + EX31_RS21760 0.27 -1.5 95,182 + EX31_RS21760 0.27 +0.5 95,183 - EX31_RS21760 0.27 -1.1 95,183 - EX31_RS21760 0.27 -0.3 95,183 - EX31_RS21760 0.27 -0.8
Or see this region's nucleotide sequence