Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18480 and EX31_RS18485 overlap by 1 nucleotides EX31_RS18485 and EX31_RS18490 are separated by 69 nucleotides EX31_RS18490 and EX31_RS18495 are separated by 1 nucleotides EX31_RS18495 and EX31_RS18500 are separated by 11 nucleotides
EX31_RS18480: EX31_RS18480 - ferredoxin-type protein NapF, at 266,132 to 266,704
_RS18480
EX31_RS18485: EX31_RS18485 - Tat proofreading chaperone DmsD, at 266,704 to 267,312
_RS18485
EX31_RS18490: EX31_RS18490 - dimethyl sulfoxide reductase anchor subunit, at 267,382 to 268,239
_RS18490
EX31_RS18495: EX31_RS18495 - dimethylsulfoxide reductase subunit B, at 268,241 to 268,858
_RS18495
EX31_RS18500: EX31_RS18500 - dimethylsulfoxide reductase subunit A, at 268,870 to 271,317
_RS18500
Position (kb) 267
268
269 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 266.392 kb on + strand, within EX31_RS18480 at 266.392 kb on + strand, within EX31_RS18480 at 266.422 kb on - strand, within EX31_RS18480 at 266.459 kb on + strand, within EX31_RS18480 at 266.459 kb on + strand, within EX31_RS18480 at 266.460 kb on - strand, within EX31_RS18480 at 266.461 kb on + strand, within EX31_RS18480 at 266.462 kb on - strand, within EX31_RS18480 at 266.462 kb on - strand, within EX31_RS18480 at 266.462 kb on - strand, within EX31_RS18480 at 266.634 kb on - strand, within EX31_RS18480 at 266.677 kb on - strand at 266.679 kb on + strand at 266.680 kb on - strand at 266.680 kb on - strand at 266.680 kb on - strand at 266.711 kb on + strand at 266.712 kb on - strand at 266.712 kb on - strand at 266.739 kb on + strand at 266.739 kb on + strand at 266.739 kb on + strand at 266.740 kb on - strand at 266.740 kb on - strand at 266.740 kb on - strand at 266.740 kb on - strand at 266.740 kb on - strand at 266.740 kb on - strand at 266.761 kb on + strand at 266.761 kb on + strand at 266.762 kb on - strand at 266.780 kb on - strand, within EX31_RS18485 at 266.855 kb on - strand, within EX31_RS18485 at 267.078 kb on - strand, within EX31_RS18485 at 267.217 kb on + strand, within EX31_RS18485 at 267.223 kb on - strand, within EX31_RS18485 at 267.223 kb on - strand, within EX31_RS18485 at 267.223 kb on - strand, within EX31_RS18485 at 267.223 kb on - strand, within EX31_RS18485 at 267.223 kb on - strand, within EX31_RS18485 at 267.226 kb on + strand, within EX31_RS18485 at 267.227 kb on - strand, within EX31_RS18485 at 267.294 kb on + strand at 267.313 kb on - strand at 267.313 kb on - strand at 267.343 kb on - strand at 267.411 kb on + strand at 267.411 kb on + strand at 267.412 kb on - strand at 267.423 kb on - strand at 267.502 kb on - strand, within EX31_RS18490 at 267.509 kb on - strand, within EX31_RS18490 at 267.509 kb on - strand, within EX31_RS18490 at 267.509 kb on - strand, within EX31_RS18490 at 267.509 kb on - strand, within EX31_RS18490 at 267.509 kb on - strand, within EX31_RS18490 at 267.509 kb on - strand, within EX31_RS18490 at 267.511 kb on - strand, within EX31_RS18490 at 267.511 kb on - strand, within EX31_RS18490 at 267.511 kb on - strand, within EX31_RS18490 at 267.511 kb on - strand, within EX31_RS18490 at 267.511 kb on - strand, within EX31_RS18490 at 267.511 kb on - strand, within EX31_RS18490 at 267.511 kb on - strand, within EX31_RS18490 at 267.579 kb on - strand, within EX31_RS18490 at 267.661 kb on - strand, within EX31_RS18490 at 267.661 kb on - strand, within EX31_RS18490 at 267.661 kb on - strand, within EX31_RS18490 at 267.704 kb on - strand, within EX31_RS18490 at 267.717 kb on + strand, within EX31_RS18490 at 267.718 kb on - strand, within EX31_RS18490 at 267.718 kb on - strand, within EX31_RS18490 at 267.718 kb on - strand, within EX31_RS18490 at 267.720 kb on + strand, within EX31_RS18490 at 267.720 kb on + strand, within EX31_RS18490 at 267.720 kb on + strand, within EX31_RS18490 at 267.721 kb on - strand, within EX31_RS18490 at 267.721 kb on - strand, within EX31_RS18490 at 267.721 kb on - strand, within EX31_RS18490 at 267.721 kb on - strand, within EX31_RS18490 at 267.721 kb on - strand, within EX31_RS18490 at 267.723 kb on + strand, within EX31_RS18490 at 267.723 kb on + strand, within EX31_RS18490 at 267.724 kb on - strand, within EX31_RS18490 at 267.724 kb on - strand, within EX31_RS18490 at 267.744 kb on - strand, within EX31_RS18490 at 267.795 kb on + strand, within EX31_RS18490 at 267.798 kb on - strand, within EX31_RS18490 at 267.798 kb on - strand, within EX31_RS18490 at 267.822 kb on + strand, within EX31_RS18490 at 267.823 kb on - strand, within EX31_RS18490 at 267.833 kb on + strand, within EX31_RS18490 at 267.833 kb on + strand, within EX31_RS18490 at 267.834 kb on - strand, within EX31_RS18490 at 267.834 kb on - strand, within EX31_RS18490 at 267.834 kb on - strand, within EX31_RS18490 at 267.834 kb on - strand, within EX31_RS18490 at 267.834 kb on - strand, within EX31_RS18490 at 267.941 kb on + strand, within EX31_RS18490 at 267.941 kb on + strand, within EX31_RS18490 at 267.941 kb on + strand, within EX31_RS18490 at 268.122 kb on - strand, within EX31_RS18490 at 268.168 kb on - strand at 268.168 kb on - strand at 268.267 kb on - strand at 268.267 kb on - strand at 268.277 kb on - strand at 268.310 kb on - strand, within EX31_RS18495 at 268.310 kb on - strand, within EX31_RS18495 at 268.319 kb on - strand, within EX31_RS18495 at 268.319 kb on - strand, within EX31_RS18495 at 268.375 kb on - strand, within EX31_RS18495 at 268.375 kb on - strand, within EX31_RS18495 at 268.470 kb on + strand, within EX31_RS18495 at 268.471 kb on - strand, within EX31_RS18495 at 268.512 kb on + strand, within EX31_RS18495 at 268.513 kb on - strand, within EX31_RS18495 at 268.513 kb on - strand, within EX31_RS18495 at 268.561 kb on + strand, within EX31_RS18495 at 268.568 kb on - strand, within EX31_RS18495 at 268.568 kb on - strand, within EX31_RS18495 at 268.568 kb on - strand, within EX31_RS18495 at 268.604 kb on - strand, within EX31_RS18495 at 268.674 kb on + strand, within EX31_RS18495 at 268.674 kb on + strand, within EX31_RS18495 at 268.678 kb on - strand, within EX31_RS18495 at 268.734 kb on + strand, within EX31_RS18495 at 268.740 kb on + strand, within EX31_RS18495 at 268.740 kb on + strand, within EX31_RS18495 at 268.740 kb on + strand, within EX31_RS18495 at 268.740 kb on + strand, within EX31_RS18495 at 268.740 kb on + strand, within EX31_RS18495 at 268.741 kb on - strand, within EX31_RS18495 at 268.741 kb on - strand, within EX31_RS18495 at 268.741 kb on - strand, within EX31_RS18495 at 268.741 kb on - strand, within EX31_RS18495 at 268.744 kb on + strand, within EX31_RS18495 at 268.834 kb on + strand at 268.836 kb on + strand at 268.837 kb on - strand at 268.900 kb on - strand at 268.900 kb on - strand at 268.912 kb on - strand at 268.948 kb on + strand at 268.949 kb on - strand at 268.949 kb on - strand at 268.949 kb on - strand at 268.949 kb on - strand at 268.949 kb on - strand at 268.949 kb on - strand at 268.953 kb on + strand at 268.954 kb on - strand at 269.056 kb on + strand at 269.057 kb on - strand at 269.058 kb on + strand at 269.058 kb on + strand at 269.058 kb on + strand at 269.058 kb on + strand at 269.059 kb on - strand at 269.059 kb on - strand at 269.109 kb on + strand at 269.171 kb on - strand, within EX31_RS18500 at 269.186 kb on + strand, within EX31_RS18500 at 269.208 kb on + strand, within EX31_RS18500 at 269.209 kb on - strand, within EX31_RS18500 at 269.210 kb on + strand, within EX31_RS18500 at 269.210 kb on + strand, within EX31_RS18500 at 269.238 kb on - strand, within EX31_RS18500 at 269.238 kb on - strand, within EX31_RS18500 at 269.238 kb on - strand, within EX31_RS18500
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 266,392 + EX31_RS18480 0.45 +0.4 266,392 + EX31_RS18480 0.45 -0.1 266,422 - EX31_RS18480 0.51 -2.1 266,459 + EX31_RS18480 0.57 +0.6 266,459 + EX31_RS18480 0.57 +1.1 266,460 - EX31_RS18480 0.57 +1.2 266,461 + EX31_RS18480 0.57 -2.1 266,462 - EX31_RS18480 0.58 +1.3 266,462 - EX31_RS18480 0.58 -1.4 266,462 - EX31_RS18480 0.58 +0.6 266,634 - EX31_RS18480 0.88 +1.3 266,677 - +1.0 266,679 + +0.9 266,680 - -0.4 266,680 - -1.4 266,680 - -0.1 266,711 + +0.0 266,712 - +0.4 266,712 - -0.6 266,739 + +1.2 266,739 + +0.9 266,739 + -0.9 266,740 - -0.5 266,740 - +0.1 266,740 - +0.4 266,740 - +0.4 266,740 - -0.6 266,740 - -0.7 266,761 + -0.1 266,761 + -1.5 266,762 - -0.3 266,780 - EX31_RS18485 0.12 -0.1 266,855 - EX31_RS18485 0.25 +0.8 267,078 - EX31_RS18485 0.61 +0.5 267,217 + EX31_RS18485 0.84 -0.6 267,223 - EX31_RS18485 0.85 -0.6 267,223 - EX31_RS18485 0.85 +0.6 267,223 - EX31_RS18485 0.85 +1.3 267,223 - EX31_RS18485 0.85 +1.8 267,223 - EX31_RS18485 0.85 +0.4 267,226 + EX31_RS18485 0.86 -1.1 267,227 - EX31_RS18485 0.86 +1.5 267,294 + +1.2 267,313 - +0.7 267,313 - +0.1 267,343 - +1.3 267,411 + +2.9 267,411 + -0.8 267,412 - +0.7 267,423 - +1.2 267,502 - EX31_RS18490 0.14 -0.2 267,509 - EX31_RS18490 0.15 +0.7 267,509 - EX31_RS18490 0.15 +2.5 267,509 - EX31_RS18490 0.15 -1.4 267,509 - EX31_RS18490 0.15 -0.2 267,509 - EX31_RS18490 0.15 -1.5 267,509 - EX31_RS18490 0.15 +0.5 267,511 - EX31_RS18490 0.15 +0.2 267,511 - EX31_RS18490 0.15 -2.1 267,511 - EX31_RS18490 0.15 +0.5 267,511 - EX31_RS18490 0.15 -0.5 267,511 - EX31_RS18490 0.15 +0.9 267,511 - EX31_RS18490 0.15 +1.1 267,511 - EX31_RS18490 0.15 -1.2 267,579 - EX31_RS18490 0.23 -2.0 267,661 - EX31_RS18490 0.33 -1.5 267,661 - EX31_RS18490 0.33 -1.2 267,661 - EX31_RS18490 0.33 -0.4 267,704 - EX31_RS18490 0.38 -1.9 267,717 + EX31_RS18490 0.39 -0.1 267,718 - EX31_RS18490 0.39 +0.4 267,718 - EX31_RS18490 0.39 +0.1 267,718 - EX31_RS18490 0.39 -0.2 267,720 + EX31_RS18490 0.39 -0.3 267,720 + EX31_RS18490 0.39 -0.9 267,720 + EX31_RS18490 0.39 +0.8 267,721 - EX31_RS18490 0.40 -0.0 267,721 - EX31_RS18490 0.40 +0.1 267,721 - EX31_RS18490 0.40 -1.6 267,721 - EX31_RS18490 0.40 -0.1 267,721 - EX31_RS18490 0.40 +1.7 267,723 + EX31_RS18490 0.40 +1.9 267,723 + EX31_RS18490 0.40 +0.4 267,724 - EX31_RS18490 0.40 -0.6 267,724 - EX31_RS18490 0.40 +2.4 267,744 - EX31_RS18490 0.42 -1.6 267,795 + EX31_RS18490 0.48 -0.9 267,798 - EX31_RS18490 0.48 +0.4 267,798 - EX31_RS18490 0.48 +0.0 267,822 + EX31_RS18490 0.51 +0.3 267,823 - EX31_RS18490 0.51 -1.2 267,833 + EX31_RS18490 0.53 -0.4 267,833 + EX31_RS18490 0.53 +2.2 267,834 - EX31_RS18490 0.53 -0.5 267,834 - EX31_RS18490 0.53 +1.4 267,834 - EX31_RS18490 0.53 -0.9 267,834 - EX31_RS18490 0.53 -0.6 267,834 - EX31_RS18490 0.53 -0.8 267,941 + EX31_RS18490 0.65 +0.1 267,941 + EX31_RS18490 0.65 +1.1 267,941 + EX31_RS18490 0.65 +1.2 268,122 - EX31_RS18490 0.86 +0.3 268,168 - -2.1 268,168 - -0.4 268,267 - -2.2 268,267 - -1.1 268,277 - -1.1 268,310 - EX31_RS18495 0.11 -0.9 268,310 - EX31_RS18495 0.11 -1.6 268,319 - EX31_RS18495 0.13 +0.4 268,319 - EX31_RS18495 0.13 +0.4 268,375 - EX31_RS18495 0.22 +0.4 268,375 - EX31_RS18495 0.22 +0.8 268,470 + EX31_RS18495 0.37 -0.1 268,471 - EX31_RS18495 0.37 +0.5 268,512 + EX31_RS18495 0.44 -0.2 268,513 - EX31_RS18495 0.44 -0.9 268,513 - EX31_RS18495 0.44 +0.9 268,561 + EX31_RS18495 0.52 +0.7 268,568 - EX31_RS18495 0.53 -0.1 268,568 - EX31_RS18495 0.53 +0.4 268,568 - EX31_RS18495 0.53 +1.1 268,604 - EX31_RS18495 0.59 -1.1 268,674 + EX31_RS18495 0.70 +0.1 268,674 + EX31_RS18495 0.70 -0.9 268,678 - EX31_RS18495 0.71 +0.2 268,734 + EX31_RS18495 0.80 -1.2 268,740 + EX31_RS18495 0.81 -0.4 268,740 + EX31_RS18495 0.81 -0.2 268,740 + EX31_RS18495 0.81 -1.1 268,740 + EX31_RS18495 0.81 +2.5 268,740 + EX31_RS18495 0.81 -0.0 268,741 - EX31_RS18495 0.81 -1.1 268,741 - EX31_RS18495 0.81 -3.4 268,741 - EX31_RS18495 0.81 -1.1 268,741 - EX31_RS18495 0.81 -0.6 268,744 + EX31_RS18495 0.81 +1.9 268,834 + +0.8 268,836 + +0.7 268,837 - +0.3 268,900 - -0.1 268,900 - +0.9 268,912 - -0.3 268,948 + +0.9 268,949 - +1.1 268,949 - +0.9 268,949 - +1.5 268,949 - +1.8 268,949 - -0.6 268,949 - -1.1 268,953 + -0.3 268,954 - +2.3 269,056 + +2.1 269,057 - -0.1 269,058 + -0.9 269,058 + +1.9 269,058 + -0.6 269,058 + -0.8 269,059 - -0.8 269,059 - +1.3 269,109 + +0.2 269,171 - EX31_RS18500 0.12 -1.9 269,186 + EX31_RS18500 0.13 +0.8 269,208 + EX31_RS18500 0.14 -0.5 269,209 - EX31_RS18500 0.14 +0.7 269,210 + EX31_RS18500 0.14 -0.4 269,210 + EX31_RS18500 0.14 +0.9 269,238 - EX31_RS18500 0.15 -0.1 269,238 - EX31_RS18500 0.15 -1.2 269,238 - EX31_RS18500 0.15 -0.4
Or see this region's nucleotide sequence