Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS17020 and EX31_RS17025 overlap by 14 nucleotides EX31_RS17025 and EX31_RS17030 are separated by 307 nucleotides
EX31_RS17020: EX31_RS17020 - aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme, at 14,925 to 16,103
_RS17020
EX31_RS17025: EX31_RS17025 - FadR family transcriptional regulator, at 16,090 to 16,803
_RS17025
EX31_RS17030: EX31_RS17030 - FAD-dependent oxidoreductase, at 17,111 to 18,319
_RS17030
Position (kb) 16
17 Strain fitness (log2 ratio) -2
-1
0
1
2 at 15.392 kb on + strand, within EX31_RS17020 at 15.392 kb on + strand, within EX31_RS17020 at 15.393 kb on - strand, within EX31_RS17020 at 15.393 kb on - strand, within EX31_RS17020 at 15.395 kb on - strand, within EX31_RS17020 at 15.395 kb on - strand, within EX31_RS17020 at 15.395 kb on - strand, within EX31_RS17020 at 15.395 kb on - strand, within EX31_RS17020 at 15.469 kb on + strand, within EX31_RS17020 at 15.470 kb on - strand, within EX31_RS17020 at 15.562 kb on - strand, within EX31_RS17020 at 15.562 kb on - strand, within EX31_RS17020 at 15.749 kb on - strand, within EX31_RS17020 at 15.973 kb on + strand, within EX31_RS17020 at 15.974 kb on - strand, within EX31_RS17020 at 16.003 kb on + strand at 16.004 kb on - strand at 16.050 kb on + strand at 16.088 kb on + strand at 16.132 kb on + strand at 16.133 kb on - strand at 16.154 kb on - strand at 16.198 kb on + strand, within EX31_RS17025 at 16.277 kb on + strand, within EX31_RS17025 at 16.277 kb on + strand, within EX31_RS17025 at 16.325 kb on + strand, within EX31_RS17025 at 16.405 kb on - strand, within EX31_RS17025 at 16.522 kb on - strand, within EX31_RS17025 at 16.522 kb on - strand, within EX31_RS17025 at 16.724 kb on + strand, within EX31_RS17025 at 16.724 kb on + strand, within EX31_RS17025 at 16.725 kb on - strand, within EX31_RS17025 at 16.725 kb on - strand, within EX31_RS17025 at 16.783 kb on + strand at 16.783 kb on + strand at 16.784 kb on - strand at 16.789 kb on + strand at 16.790 kb on - strand at 16.790 kb on - strand at 17.033 kb on + strand at 17.033 kb on + strand at 17.033 kb on + strand at 17.033 kb on + strand at 17.033 kb on + strand at 17.179 kb on - strand at 17.189 kb on + strand at 17.281 kb on + strand, within EX31_RS17030 at 17.282 kb on - strand, within EX31_RS17030 at 17.425 kb on + strand, within EX31_RS17030 at 17.537 kb on - strand, within EX31_RS17030 at 17.537 kb on - strand, within EX31_RS17030 at 17.537 kb on - strand, within EX31_RS17030 at 17.688 kb on + strand, within EX31_RS17030
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 15,392 + EX31_RS17020 0.40 +0.5 15,392 + EX31_RS17020 0.40 +1.4 15,393 - EX31_RS17020 0.40 -1.5 15,393 - EX31_RS17020 0.40 +0.5 15,395 - EX31_RS17020 0.40 -1.7 15,395 - EX31_RS17020 0.40 -0.9 15,395 - EX31_RS17020 0.40 +0.9 15,395 - EX31_RS17020 0.40 +1.2 15,469 + EX31_RS17020 0.46 +0.5 15,470 - EX31_RS17020 0.46 +1.8 15,562 - EX31_RS17020 0.54 -0.5 15,562 - EX31_RS17020 0.54 +0.5 15,749 - EX31_RS17020 0.70 +0.1 15,973 + EX31_RS17020 0.89 +0.5 15,974 - EX31_RS17020 0.89 +0.3 16,003 + +1.5 16,004 - +0.6 16,050 + -0.4 16,088 + +1.5 16,132 + +0.1 16,133 - +0.4 16,154 - +0.0 16,198 + EX31_RS17025 0.15 +1.5 16,277 + EX31_RS17025 0.26 +0.1 16,277 + EX31_RS17025 0.26 -0.3 16,325 + EX31_RS17025 0.33 -0.8 16,405 - EX31_RS17025 0.44 -1.2 16,522 - EX31_RS17025 0.61 +0.1 16,522 - EX31_RS17025 0.61 +0.0 16,724 + EX31_RS17025 0.89 -1.7 16,724 + EX31_RS17025 0.89 -1.1 16,725 - EX31_RS17025 0.89 -1.7 16,725 - EX31_RS17025 0.89 -2.3 16,783 + -0.0 16,783 + +0.7 16,784 - +0.8 16,789 + +0.6 16,790 - +0.9 16,790 - -1.1 17,033 + +0.1 17,033 + +0.7 17,033 + +0.1 17,033 + +0.9 17,033 + -1.7 17,179 - -0.7 17,189 + -1.4 17,281 + EX31_RS17030 0.14 -0.0 17,282 - EX31_RS17030 0.14 +0.8 17,425 + EX31_RS17030 0.26 +1.5 17,537 - EX31_RS17030 0.35 -0.7 17,537 - EX31_RS17030 0.35 +0.8 17,537 - EX31_RS17030 0.35 +0.8 17,688 + EX31_RS17030 0.48 -0.5
Or see this region's nucleotide sequence