Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12155 and EX31_RS12160 are separated by 417 nucleotides EX31_RS12160 and EX31_RS12165 are separated by 86 nucleotides
EX31_RS12155: EX31_RS12155 - amino acid ABC transporter substrate-binding protein, at 98,770 to 99,795
_RS12155
EX31_RS12160: EX31_RS12160 - hypothetical protein, at 100,213 to 100,398
_RS12160
EX31_RS12165: EX31_RS12165 - kdo(2)-lipid IV(A) palmitoleoyltransferase, at 100,485 to 101,405
_RS12165
Position (kb) 100
101 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 99.281 kb on + strand, within EX31_RS12155 at 99.281 kb on + strand, within EX31_RS12155 at 99.281 kb on + strand, within EX31_RS12155 at 99.282 kb on - strand, within EX31_RS12155 at 99.282 kb on - strand, within EX31_RS12155 at 99.282 kb on - strand, within EX31_RS12155 at 99.282 kb on - strand, within EX31_RS12155 at 99.282 kb on - strand, within EX31_RS12155 at 99.305 kb on + strand, within EX31_RS12155 at 99.306 kb on - strand, within EX31_RS12155 at 99.306 kb on - strand, within EX31_RS12155 at 99.422 kb on + strand, within EX31_RS12155 at 99.422 kb on + strand, within EX31_RS12155 at 99.422 kb on + strand, within EX31_RS12155 at 99.423 kb on - strand, within EX31_RS12155 at 99.423 kb on - strand, within EX31_RS12155 at 99.423 kb on - strand, within EX31_RS12155 at 99.425 kb on + strand, within EX31_RS12155 at 99.425 kb on + strand, within EX31_RS12155 at 99.425 kb on + strand, within EX31_RS12155 at 99.425 kb on + strand, within EX31_RS12155 at 99.426 kb on - strand, within EX31_RS12155 at 99.426 kb on - strand, within EX31_RS12155 at 99.426 kb on - strand, within EX31_RS12155 at 99.430 kb on + strand, within EX31_RS12155 at 99.486 kb on + strand, within EX31_RS12155 at 99.486 kb on + strand, within EX31_RS12155 at 99.487 kb on - strand, within EX31_RS12155 at 99.487 kb on - strand, within EX31_RS12155 at 99.554 kb on + strand, within EX31_RS12155 at 99.554 kb on + strand, within EX31_RS12155 at 99.554 kb on + strand, within EX31_RS12155 at 99.555 kb on - strand, within EX31_RS12155 at 99.555 kb on - strand, within EX31_RS12155 at 99.647 kb on + strand, within EX31_RS12155 at 99.647 kb on + strand, within EX31_RS12155 at 99.647 kb on + strand, within EX31_RS12155 at 99.648 kb on - strand, within EX31_RS12155 at 99.648 kb on - strand, within EX31_RS12155 at 99.648 kb on - strand, within EX31_RS12155 at 99.648 kb on - strand, within EX31_RS12155 at 99.784 kb on + strand at 99.785 kb on - strand at 99.796 kb on - strand at 99.825 kb on + strand at 99.826 kb on - strand at 99.826 kb on - strand at 99.828 kb on + strand at 99.828 kb on + strand at 99.835 kb on + strand at 99.835 kb on + strand at 99.835 kb on + strand at 99.836 kb on - strand at 99.836 kb on - strand at 99.836 kb on - strand at 99.836 kb on - strand at 99.936 kb on + strand at 99.936 kb on + strand at 99.936 kb on + strand at 99.936 kb on + strand at 99.936 kb on + strand at 99.936 kb on + strand at 99.937 kb on - strand at 99.952 kb on + strand at 99.971 kb on - strand at 99.978 kb on - strand at 100.015 kb on + strand at 100.087 kb on + strand at 100.087 kb on + strand at 100.088 kb on - strand at 100.088 kb on - strand at 100.119 kb on + strand at 100.119 kb on + strand at 100.120 kb on - strand at 100.120 kb on - strand at 100.120 kb on - strand at 100.120 kb on - strand at 100.120 kb on - strand at 100.120 kb on - strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.149 kb on + strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.150 kb on - strand at 100.169 kb on + strand at 100.169 kb on + strand at 100.170 kb on - strand at 100.184 kb on + strand at 100.184 kb on + strand at 100.184 kb on + strand at 100.184 kb on + strand at 100.185 kb on - strand at 100.185 kb on - strand at 100.214 kb on + strand at 100.214 kb on + strand at 100.215 kb on - strand at 100.215 kb on - strand at 100.215 kb on - strand at 100.215 kb on - strand at 100.215 kb on - strand at 100.215 kb on - strand at 100.215 kb on - strand at 100.285 kb on - strand, within EX31_RS12160 at 100.285 kb on - strand, within EX31_RS12160 at 100.285 kb on - strand, within EX31_RS12160 at 100.285 kb on - strand, within EX31_RS12160 at 100.368 kb on + strand, within EX31_RS12160 at 100.368 kb on + strand, within EX31_RS12160 at 100.368 kb on + strand, within EX31_RS12160 at 100.368 kb on + strand, within EX31_RS12160 at 100.368 kb on + strand, within EX31_RS12160 at 100.368 kb on + strand, within EX31_RS12160 at 100.368 kb on + strand, within EX31_RS12160 at 100.368 kb on + strand, within EX31_RS12160 at 100.369 kb on - strand, within EX31_RS12160 at 100.369 kb on - strand, within EX31_RS12160 at 100.369 kb on - strand, within EX31_RS12160 at 100.405 kb on + strand at 100.406 kb on - strand at 100.486 kb on + strand at 100.487 kb on - strand at 100.524 kb on - strand at 100.546 kb on + strand at 100.547 kb on - strand at 100.547 kb on - strand at 100.547 kb on - strand at 100.565 kb on + strand at 100.565 kb on + strand at 100.566 kb on - strand at 100.566 kb on - strand at 100.594 kb on + strand, within EX31_RS12165 at 100.594 kb on + strand, within EX31_RS12165 at 100.594 kb on + strand, within EX31_RS12165 at 100.594 kb on + strand, within EX31_RS12165 at 100.595 kb on - strand, within EX31_RS12165 at 100.595 kb on - strand, within EX31_RS12165 at 100.679 kb on + strand, within EX31_RS12165 at 100.679 kb on + strand, within EX31_RS12165 at 100.679 kb on + strand, within EX31_RS12165 at 100.679 kb on + strand, within EX31_RS12165 at 100.679 kb on + strand, within EX31_RS12165 at 100.680 kb on - strand, within EX31_RS12165 at 100.680 kb on - strand, within EX31_RS12165 at 100.680 kb on - strand, within EX31_RS12165 at 100.680 kb on - strand, within EX31_RS12165 at 100.683 kb on + strand, within EX31_RS12165 at 100.730 kb on + strand, within EX31_RS12165 at 100.746 kb on - strand, within EX31_RS12165 at 100.782 kb on + strand, within EX31_RS12165 at 100.783 kb on - strand, within EX31_RS12165 at 100.783 kb on - strand, within EX31_RS12165 at 100.801 kb on + strand, within EX31_RS12165 at 100.893 kb on - strand, within EX31_RS12165 at 100.893 kb on - strand, within EX31_RS12165 at 100.901 kb on + strand, within EX31_RS12165 at 100.901 kb on + strand, within EX31_RS12165 at 100.902 kb on - strand, within EX31_RS12165 at 100.902 kb on - strand, within EX31_RS12165 at 100.902 kb on - strand, within EX31_RS12165 at 100.934 kb on + strand, within EX31_RS12165 at 100.934 kb on + strand, within EX31_RS12165 at 100.935 kb on - strand, within EX31_RS12165 at 100.935 kb on - strand, within EX31_RS12165 at 100.935 kb on - strand, within EX31_RS12165 at 100.935 kb on - strand, within EX31_RS12165 at 100.935 kb on - strand, within EX31_RS12165 at 100.935 kb on - strand, within EX31_RS12165 at 100.951 kb on + strand, within EX31_RS12165 at 100.952 kb on - strand, within EX31_RS12165 at 100.952 kb on - strand, within EX31_RS12165 at 100.974 kb on + strand, within EX31_RS12165 at 100.974 kb on + strand, within EX31_RS12165 at 100.974 kb on + strand, within EX31_RS12165 at 100.974 kb on + strand, within EX31_RS12165 at 100.975 kb on - strand, within EX31_RS12165 at 100.975 kb on - strand, within EX31_RS12165 at 100.975 kb on - strand, within EX31_RS12165 at 100.975 kb on - strand, within EX31_RS12165 at 100.975 kb on - strand, within EX31_RS12165 at 101.009 kb on + strand, within EX31_RS12165 at 101.009 kb on + strand, within EX31_RS12165 at 101.010 kb on - strand, within EX31_RS12165 at 101.010 kb on - strand, within EX31_RS12165 at 101.010 kb on - strand, within EX31_RS12165 at 101.010 kb on - strand, within EX31_RS12165 at 101.010 kb on - strand, within EX31_RS12165 at 101.299 kb on - strand, within EX31_RS12165 at 101.334 kb on + strand at 101.335 kb on - strand at 101.373 kb on + strand at 101.373 kb on + strand at 101.373 kb on + strand at 101.373 kb on + strand at 101.374 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 99,281 + EX31_RS12155 0.50 -0.7 99,281 + EX31_RS12155 0.50 +0.7 99,281 + EX31_RS12155 0.50 -0.2 99,282 - EX31_RS12155 0.50 +0.2 99,282 - EX31_RS12155 0.50 -1.1 99,282 - EX31_RS12155 0.50 +0.1 99,282 - EX31_RS12155 0.50 -0.5 99,282 - EX31_RS12155 0.50 -0.3 99,305 + EX31_RS12155 0.52 -0.4 99,306 - EX31_RS12155 0.52 +1.4 99,306 - EX31_RS12155 0.52 +1.3 99,422 + EX31_RS12155 0.64 +0.4 99,422 + EX31_RS12155 0.64 +0.1 99,422 + EX31_RS12155 0.64 -0.4 99,423 - EX31_RS12155 0.64 -0.5 99,423 - EX31_RS12155 0.64 -0.7 99,423 - EX31_RS12155 0.64 +1.4 99,425 + EX31_RS12155 0.64 -0.0 99,425 + EX31_RS12155 0.64 -2.1 99,425 + EX31_RS12155 0.64 +1.6 99,425 + EX31_RS12155 0.64 -1.0 99,426 - EX31_RS12155 0.64 +0.9 99,426 - EX31_RS12155 0.64 +1.2 99,426 - EX31_RS12155 0.64 +1.3 99,430 + EX31_RS12155 0.64 -0.6 99,486 + EX31_RS12155 0.70 -1.9 99,486 + EX31_RS12155 0.70 +1.6 99,487 - EX31_RS12155 0.70 -0.3 99,487 - EX31_RS12155 0.70 -1.0 99,554 + EX31_RS12155 0.76 -0.1 99,554 + EX31_RS12155 0.76 +1.6 99,554 + EX31_RS12155 0.76 -0.5 99,555 - EX31_RS12155 0.77 -1.2 99,555 - EX31_RS12155 0.77 -0.0 99,647 + EX31_RS12155 0.85 +0.2 99,647 + EX31_RS12155 0.85 -0.4 99,647 + EX31_RS12155 0.85 -0.5 99,648 - EX31_RS12155 0.86 +0.3 99,648 - EX31_RS12155 0.86 -0.5 99,648 - EX31_RS12155 0.86 +1.5 99,648 - EX31_RS12155 0.86 +0.3 99,784 + +0.9 99,785 - -1.2 99,796 - -0.4 99,825 + -0.4 99,826 - -0.3 99,826 - +0.0 99,828 + -2.8 99,828 + -0.3 99,835 + +1.3 99,835 + -1.2 99,835 + -1.9 99,836 - +0.9 99,836 - -1.0 99,836 - -0.0 99,836 - +0.3 99,936 + -1.0 99,936 + -0.2 99,936 + +0.5 99,936 + +1.1 99,936 + -0.8 99,936 + +0.0 99,937 - -1.4 99,952 + +0.2 99,971 - +0.9 99,978 - -0.3 100,015 + -1.2 100,087 + +0.0 100,087 + +0.5 100,088 - +0.9 100,088 - -0.7 100,119 + -1.1 100,119 + -0.5 100,120 - +0.4 100,120 - +0.2 100,120 - -0.1 100,120 - +0.4 100,120 - +1.3 100,120 - +0.4 100,149 + +0.1 100,149 + +0.2 100,149 + -1.9 100,149 + +0.3 100,149 + -0.3 100,149 + -0.1 100,149 + -1.5 100,149 + +0.8 100,149 + +0.1 100,149 + +0.0 100,149 + -0.3 100,149 + +0.2 100,149 + +0.0 100,149 + -0.4 100,149 + -1.0 100,149 + -1.9 100,149 + -0.5 100,149 + +1.9 100,149 + -0.4 100,149 + -0.3 100,149 + +0.5 100,149 + +0.5 100,149 + +0.1 100,149 + +0.5 100,149 + -0.1 100,149 + -0.6 100,149 + -0.4 100,149 + -0.1 100,149 + +0.8 100,150 - -0.5 100,150 - -0.9 100,150 - +0.2 100,150 - +0.9 100,150 - -0.7 100,150 - +0.4 100,150 - +1.0 100,150 - -0.4 100,150 - +1.2 100,150 - -0.2 100,150 - -0.1 100,150 - +0.7 100,150 - +0.7 100,150 - -0.3 100,150 - -1.1 100,150 - +0.8 100,150 - +1.9 100,150 - +1.1 100,169 + +0.6 100,169 + -0.6 100,170 - +1.2 100,184 + +1.0 100,184 + -0.6 100,184 + -0.7 100,184 + +0.7 100,185 - +0.5 100,185 - -0.7 100,214 + +0.6 100,214 + -1.0 100,215 - +0.7 100,215 - -0.7 100,215 - -0.3 100,215 - +0.3 100,215 - +2.5 100,215 - +1.1 100,215 - +0.6 100,285 - EX31_RS12160 0.39 +0.8 100,285 - EX31_RS12160 0.39 -1.2 100,285 - EX31_RS12160 0.39 -0.1 100,285 - EX31_RS12160 0.39 +0.2 100,368 + EX31_RS12160 0.83 +0.6 100,368 + EX31_RS12160 0.83 -0.1 100,368 + EX31_RS12160 0.83 -0.3 100,368 + EX31_RS12160 0.83 +0.1 100,368 + EX31_RS12160 0.83 -0.1 100,368 + EX31_RS12160 0.83 +0.2 100,368 + EX31_RS12160 0.83 -0.6 100,368 + EX31_RS12160 0.83 -0.8 100,369 - EX31_RS12160 0.84 +1.8 100,369 - EX31_RS12160 0.84 +0.7 100,369 - EX31_RS12160 0.84 +0.4 100,405 + +0.5 100,406 - +0.8 100,486 + -0.6 100,487 - +0.3 100,524 - +1.1 100,546 + -0.9 100,547 - -0.8 100,547 - +1.3 100,547 - +0.1 100,565 + +0.1 100,565 + -0.4 100,566 - -0.1 100,566 - -1.0 100,594 + EX31_RS12165 0.12 +0.9 100,594 + EX31_RS12165 0.12 -0.7 100,594 + EX31_RS12165 0.12 +0.8 100,594 + EX31_RS12165 0.12 -0.6 100,595 - EX31_RS12165 0.12 -1.6 100,595 - EX31_RS12165 0.12 -0.8 100,679 + EX31_RS12165 0.21 -0.1 100,679 + EX31_RS12165 0.21 -0.2 100,679 + EX31_RS12165 0.21 +0.2 100,679 + EX31_RS12165 0.21 +0.1 100,679 + EX31_RS12165 0.21 -0.3 100,680 - EX31_RS12165 0.21 +0.4 100,680 - EX31_RS12165 0.21 -0.2 100,680 - EX31_RS12165 0.21 +0.9 100,680 - EX31_RS12165 0.21 +0.9 100,683 + EX31_RS12165 0.21 +0.1 100,730 + EX31_RS12165 0.27 -1.6 100,746 - EX31_RS12165 0.28 -0.5 100,782 + EX31_RS12165 0.32 +2.2 100,783 - EX31_RS12165 0.32 -0.2 100,783 - EX31_RS12165 0.32 +0.3 100,801 + EX31_RS12165 0.34 +0.7 100,893 - EX31_RS12165 0.44 +0.4 100,893 - EX31_RS12165 0.44 +0.3 100,901 + EX31_RS12165 0.45 +1.4 100,901 + EX31_RS12165 0.45 +0.6 100,902 - EX31_RS12165 0.45 -0.5 100,902 - EX31_RS12165 0.45 -1.9 100,902 - EX31_RS12165 0.45 +1.1 100,934 + EX31_RS12165 0.49 +0.1 100,934 + EX31_RS12165 0.49 -3.1 100,935 - EX31_RS12165 0.49 +1.2 100,935 - EX31_RS12165 0.49 +0.4 100,935 - EX31_RS12165 0.49 +0.3 100,935 - EX31_RS12165 0.49 +0.6 100,935 - EX31_RS12165 0.49 +1.5 100,935 - EX31_RS12165 0.49 -0.4 100,951 + EX31_RS12165 0.51 +0.5 100,952 - EX31_RS12165 0.51 +0.2 100,952 - EX31_RS12165 0.51 -1.0 100,974 + EX31_RS12165 0.53 +1.2 100,974 + EX31_RS12165 0.53 +3.5 100,974 + EX31_RS12165 0.53 +0.3 100,974 + EX31_RS12165 0.53 +2.2 100,975 - EX31_RS12165 0.53 +1.3 100,975 - EX31_RS12165 0.53 -0.2 100,975 - EX31_RS12165 0.53 +0.3 100,975 - EX31_RS12165 0.53 +0.4 100,975 - EX31_RS12165 0.53 -1.2 101,009 + EX31_RS12165 0.57 -0.2 101,009 + EX31_RS12165 0.57 -0.1 101,010 - EX31_RS12165 0.57 +0.1 101,010 - EX31_RS12165 0.57 +0.7 101,010 - EX31_RS12165 0.57 +0.3 101,010 - EX31_RS12165 0.57 -0.1 101,010 - EX31_RS12165 0.57 -1.0 101,299 - EX31_RS12165 0.88 +1.4 101,334 + -0.3 101,335 - -1.5 101,373 + -0.8 101,373 + -0.9 101,373 + -0.0 101,373 + -2.9 101,374 - +0.9
Or see this region's nucleotide sequence