Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12025 and EX31_RS12030 are separated by 37 nucleotides EX31_RS12030 and EX31_RS12035 are separated by 54 nucleotides EX31_RS12035 and EX31_RS12040 are separated by 121 nucleotides EX31_RS12040 and EX31_RS12045 are separated by 10 nucleotides
EX31_RS12025: EX31_RS12025 - hypothetical protein, at 70,429 to 72,015
_RS12025
EX31_RS12030: EX31_RS12030 - hypothetical protein, at 72,053 to 72,538
_RS12030
EX31_RS12035: EX31_RS12035 - LysR family transcriptional regulator, at 72,593 to 73,495
_RS12035
EX31_RS12040: EX31_RS12040 - SDR family oxidoreductase, at 73,617 to 74,357
_RS12040
EX31_RS12045: EX31_RS12045 - tautomerase family protein, at 74,368 to 74,748
_RS12045
Position (kb) 72
73
74 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 71.617 kb on + strand, within EX31_RS12025 at 71.618 kb on - strand, within EX31_RS12025 at 71.689 kb on + strand, within EX31_RS12025 at 71.731 kb on + strand, within EX31_RS12025 at 71.790 kb on + strand, within EX31_RS12025 at 71.790 kb on + strand, within EX31_RS12025 at 71.790 kb on + strand, within EX31_RS12025 at 71.790 kb on + strand, within EX31_RS12025 at 71.791 kb on - strand, within EX31_RS12025 at 71.791 kb on - strand, within EX31_RS12025 at 71.800 kb on + strand, within EX31_RS12025 at 71.800 kb on + strand, within EX31_RS12025 at 71.800 kb on + strand, within EX31_RS12025 at 71.801 kb on - strand, within EX31_RS12025 at 71.801 kb on - strand, within EX31_RS12025 at 71.801 kb on - strand, within EX31_RS12025 at 71.895 kb on + strand at 71.924 kb on - strand at 71.963 kb on + strand at 71.963 kb on + strand at 71.963 kb on + strand at 71.964 kb on - strand at 71.964 kb on - strand at 71.964 kb on - strand at 71.964 kb on - strand at 72.008 kb on + strand at 72.009 kb on - strand at 72.013 kb on + strand at 72.013 kb on + strand at 72.014 kb on - strand at 72.014 kb on - strand at 72.014 kb on - strand at 72.014 kb on - strand at 72.014 kb on - strand at 72.014 kb on - strand at 72.014 kb on - strand at 72.014 kb on - strand at 72.014 kb on - strand at 72.014 kb on - strand at 72.017 kb on + strand at 72.017 kb on + strand at 72.017 kb on + strand at 72.017 kb on + strand at 72.018 kb on - strand at 72.047 kb on + strand at 72.054 kb on + strand at 72.054 kb on + strand at 72.054 kb on + strand at 72.054 kb on + strand at 72.054 kb on + strand at 72.054 kb on + strand at 72.055 kb on - strand at 72.055 kb on - strand at 72.055 kb on - strand at 72.055 kb on - strand at 72.055 kb on - strand at 72.055 kb on - strand at 72.109 kb on + strand, within EX31_RS12030 at 72.161 kb on - strand, within EX31_RS12030 at 72.313 kb on + strand, within EX31_RS12030 at 72.461 kb on - strand, within EX31_RS12030 at 72.479 kb on - strand, within EX31_RS12030 at 72.487 kb on + strand, within EX31_RS12030 at 72.521 kb on + strand at 72.521 kb on + strand at 72.521 kb on + strand at 72.521 kb on + strand at 72.522 kb on - strand at 72.522 kb on - strand at 72.522 kb on - strand at 72.522 kb on - strand at 72.522 kb on - strand at 72.522 kb on - strand at 72.522 kb on - strand at 72.556 kb on - strand at 72.556 kb on - strand at 72.592 kb on - strand at 72.643 kb on + strand at 72.676 kb on + strand at 72.788 kb on - strand, within EX31_RS12035 at 72.990 kb on + strand, within EX31_RS12035 at 72.991 kb on - strand, within EX31_RS12035 at 72.991 kb on - strand, within EX31_RS12035 at 72.991 kb on - strand, within EX31_RS12035 at 73.131 kb on + strand, within EX31_RS12035 at 73.132 kb on - strand, within EX31_RS12035 at 73.132 kb on - strand, within EX31_RS12035 at 73.132 kb on - strand, within EX31_RS12035 at 73.132 kb on - strand, within EX31_RS12035 at 73.132 kb on - strand, within EX31_RS12035 at 73.132 kb on - strand, within EX31_RS12035 at 73.224 kb on - strand, within EX31_RS12035 at 73.282 kb on + strand, within EX31_RS12035 at 73.283 kb on - strand, within EX31_RS12035 at 73.298 kb on + strand, within EX31_RS12035 at 73.299 kb on + strand, within EX31_RS12035 at 73.299 kb on + strand, within EX31_RS12035 at 73.299 kb on + strand, within EX31_RS12035 at 73.302 kb on + strand, within EX31_RS12035 at 73.302 kb on + strand, within EX31_RS12035 at 73.302 kb on + strand, within EX31_RS12035 at 73.303 kb on - strand, within EX31_RS12035 at 73.303 kb on - strand, within EX31_RS12035 at 73.342 kb on + strand, within EX31_RS12035 at 73.384 kb on + strand, within EX31_RS12035 at 73.384 kb on + strand, within EX31_RS12035 at 73.384 kb on + strand, within EX31_RS12035 at 73.385 kb on - strand, within EX31_RS12035 at 73.385 kb on - strand, within EX31_RS12035 at 73.469 kb on + strand at 73.470 kb on - strand at 73.470 kb on - strand at 73.470 kb on - strand at 73.489 kb on + strand at 73.489 kb on + strand at 73.489 kb on + strand at 73.489 kb on + strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.532 kb on + strand at 73.725 kb on + strand, within EX31_RS12040 at 73.726 kb on - strand, within EX31_RS12040 at 73.726 kb on - strand, within EX31_RS12040 at 73.726 kb on - strand, within EX31_RS12040 at 73.726 kb on - strand, within EX31_RS12040 at 73.726 kb on - strand, within EX31_RS12040 at 73.886 kb on + strand, within EX31_RS12040 at 73.887 kb on - strand, within EX31_RS12040 at 73.887 kb on - strand, within EX31_RS12040 at 73.895 kb on + strand, within EX31_RS12040 at 73.898 kb on + strand, within EX31_RS12040 at 73.898 kb on + strand, within EX31_RS12040 at 73.899 kb on - strand, within EX31_RS12040 at 73.899 kb on - strand, within EX31_RS12040 at 73.899 kb on - strand, within EX31_RS12040 at 73.899 kb on - strand, within EX31_RS12040 at 74.021 kb on + strand, within EX31_RS12040 at 74.021 kb on + strand, within EX31_RS12040 at 74.021 kb on + strand, within EX31_RS12040 at 74.021 kb on + strand, within EX31_RS12040 at 74.021 kb on + strand, within EX31_RS12040 at 74.021 kb on + strand, within EX31_RS12040 at 74.022 kb on - strand, within EX31_RS12040 at 74.022 kb on - strand, within EX31_RS12040 at 74.022 kb on - strand, within EX31_RS12040 at 74.022 kb on - strand, within EX31_RS12040 at 74.022 kb on - strand, within EX31_RS12040 at 74.022 kb on - strand, within EX31_RS12040 at 74.022 kb on - strand, within EX31_RS12040 at 74.022 kb on - strand, within EX31_RS12040 at 74.053 kb on + strand, within EX31_RS12040 at 74.053 kb on + strand, within EX31_RS12040 at 74.054 kb on - strand, within EX31_RS12040 at 74.055 kb on + strand, within EX31_RS12040 at 74.055 kb on + strand, within EX31_RS12040 at 74.055 kb on + strand, within EX31_RS12040 at 74.055 kb on + strand, within EX31_RS12040 at 74.055 kb on + strand, within EX31_RS12040 at 74.055 kb on + strand, within EX31_RS12040 at 74.056 kb on - strand, within EX31_RS12040 at 74.056 kb on - strand, within EX31_RS12040 at 74.056 kb on - strand, within EX31_RS12040 at 74.056 kb on - strand, within EX31_RS12040 at 74.056 kb on - strand, within EX31_RS12040 at 74.067 kb on + strand, within EX31_RS12040 at 74.068 kb on - strand, within EX31_RS12040 at 74.068 kb on - strand, within EX31_RS12040 at 74.068 kb on - strand, within EX31_RS12040 at 74.068 kb on - strand, within EX31_RS12040 at 74.068 kb on - strand, within EX31_RS12040 at 74.076 kb on + strand, within EX31_RS12040 at 74.077 kb on - strand, within EX31_RS12040 at 74.077 kb on - strand, within EX31_RS12040 at 74.077 kb on - strand, within EX31_RS12040 at 74.077 kb on - strand, within EX31_RS12040 at 74.146 kb on + strand, within EX31_RS12040 at 74.156 kb on + strand, within EX31_RS12040 at 74.156 kb on + strand, within EX31_RS12040 at 74.157 kb on - strand, within EX31_RS12040 at 74.157 kb on - strand, within EX31_RS12040 at 74.157 kb on - strand, within EX31_RS12040 at 74.188 kb on + strand, within EX31_RS12040 at 74.189 kb on - strand, within EX31_RS12040 at 74.189 kb on - strand, within EX31_RS12040 at 74.352 kb on - strand at 74.355 kb on + strand at 74.355 kb on + strand at 74.355 kb on + strand at 74.375 kb on + strand at 74.376 kb on - strand at 74.452 kb on + strand, within EX31_RS12045 at 74.452 kb on + strand, within EX31_RS12045 at 74.452 kb on + strand, within EX31_RS12045 at 74.453 kb on - strand, within EX31_RS12045 at 74.492 kb on + strand, within EX31_RS12045 at 74.492 kb on + strand, within EX31_RS12045 at 74.492 kb on + strand, within EX31_RS12045 at 74.492 kb on + strand, within EX31_RS12045 at 74.493 kb on - strand, within EX31_RS12045 at 74.493 kb on - strand, within EX31_RS12045 at 74.493 kb on - strand, within EX31_RS12045
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 71,617 + EX31_RS12025 0.75 +0.4 71,618 - EX31_RS12025 0.75 +1.2 71,689 + EX31_RS12025 0.79 -0.2 71,731 + EX31_RS12025 0.82 -2.6 71,790 + EX31_RS12025 0.86 +1.0 71,790 + EX31_RS12025 0.86 +0.2 71,790 + EX31_RS12025 0.86 +0.2 71,790 + EX31_RS12025 0.86 +0.1 71,791 - EX31_RS12025 0.86 -1.4 71,791 - EX31_RS12025 0.86 -0.2 71,800 + EX31_RS12025 0.86 -1.1 71,800 + EX31_RS12025 0.86 +0.3 71,800 + EX31_RS12025 0.86 +0.2 71,801 - EX31_RS12025 0.86 -0.9 71,801 - EX31_RS12025 0.86 +0.1 71,801 - EX31_RS12025 0.86 +1.0 71,895 + -0.3 71,924 - +1.4 71,963 + -1.1 71,963 + +0.1 71,963 + +1.4 71,964 - +0.1 71,964 - +0.6 71,964 - -0.4 71,964 - -0.9 72,008 + -1.7 72,009 - -2.0 72,013 + +0.9 72,013 + -0.2 72,014 - -0.8 72,014 - -0.7 72,014 - +0.5 72,014 - +0.6 72,014 - +1.6 72,014 - -0.5 72,014 - +0.5 72,014 - +0.6 72,014 - -0.5 72,014 - -0.0 72,017 + +0.0 72,017 + +1.1 72,017 + +0.5 72,017 + +0.2 72,018 - +0.3 72,047 + +1.7 72,054 + +0.7 72,054 + -2.1 72,054 + +0.8 72,054 + +0.0 72,054 + +1.0 72,054 + -0.8 72,055 - -0.5 72,055 - -0.1 72,055 - +0.5 72,055 - +0.2 72,055 - +0.7 72,055 - -0.4 72,109 + EX31_RS12030 0.12 +0.3 72,161 - EX31_RS12030 0.22 -0.5 72,313 + EX31_RS12030 0.53 +1.1 72,461 - EX31_RS12030 0.84 -0.8 72,479 - EX31_RS12030 0.88 +0.3 72,487 + EX31_RS12030 0.89 -0.2 72,521 + +0.0 72,521 + -0.1 72,521 + -0.3 72,521 + -0.1 72,522 - +0.2 72,522 - +0.2 72,522 - +0.3 72,522 - +0.4 72,522 - +0.5 72,522 - -0.6 72,522 - +0.1 72,556 - +1.4 72,556 - -1.8 72,592 - -0.4 72,643 + -0.6 72,676 + -0.7 72,788 - EX31_RS12035 0.22 -0.0 72,990 + EX31_RS12035 0.44 +0.2 72,991 - EX31_RS12035 0.44 +0.4 72,991 - EX31_RS12035 0.44 -0.9 72,991 - EX31_RS12035 0.44 +0.7 73,131 + EX31_RS12035 0.60 -1.3 73,132 - EX31_RS12035 0.60 +0.2 73,132 - EX31_RS12035 0.60 -1.1 73,132 - EX31_RS12035 0.60 +1.1 73,132 - EX31_RS12035 0.60 -1.2 73,132 - EX31_RS12035 0.60 -2.4 73,132 - EX31_RS12035 0.60 -2.0 73,224 - EX31_RS12035 0.70 +0.4 73,282 + EX31_RS12035 0.76 +0.6 73,283 - EX31_RS12035 0.76 +0.6 73,298 + EX31_RS12035 0.78 +0.4 73,299 + EX31_RS12035 0.78 +0.9 73,299 + EX31_RS12035 0.78 +0.2 73,299 + EX31_RS12035 0.78 +3.5 73,302 + EX31_RS12035 0.79 +0.2 73,302 + EX31_RS12035 0.79 +0.3 73,302 + EX31_RS12035 0.79 +0.5 73,303 - EX31_RS12035 0.79 -0.0 73,303 - EX31_RS12035 0.79 +0.1 73,342 + EX31_RS12035 0.83 +0.1 73,384 + EX31_RS12035 0.88 -1.3 73,384 + EX31_RS12035 0.88 +0.7 73,384 + EX31_RS12035 0.88 -0.9 73,385 - EX31_RS12035 0.88 -0.0 73,385 - EX31_RS12035 0.88 -0.6 73,469 + +0.3 73,470 - +1.5 73,470 - -1.5 73,470 - +0.0 73,489 + -0.7 73,489 + +0.8 73,489 + +0.2 73,489 + +1.1 73,490 - -0.5 73,490 - +0.4 73,532 + -0.2 73,725 + EX31_RS12040 0.15 -0.1 73,726 - EX31_RS12040 0.15 -0.3 73,726 - EX31_RS12040 0.15 -3.2 73,726 - EX31_RS12040 0.15 +0.1 73,726 - EX31_RS12040 0.15 +0.1 73,726 - EX31_RS12040 0.15 +0.2 73,886 + EX31_RS12040 0.36 +1.6 73,887 - EX31_RS12040 0.36 -0.7 73,887 - EX31_RS12040 0.36 +0.2 73,895 + EX31_RS12040 0.38 +0.9 73,898 + EX31_RS12040 0.38 +0.3 73,898 + EX31_RS12040 0.38 +0.2 73,899 - EX31_RS12040 0.38 +0.7 73,899 - EX31_RS12040 0.38 +0.2 73,899 - EX31_RS12040 0.38 +1.1 73,899 - EX31_RS12040 0.38 -0.5 74,021 + EX31_RS12040 0.55 -0.6 74,021 + EX31_RS12040 0.55 -0.1 74,021 + EX31_RS12040 0.55 -0.1 74,021 + EX31_RS12040 0.55 -0.2 74,021 + EX31_RS12040 0.55 -2.2 74,021 + EX31_RS12040 0.55 -0.2 74,022 - EX31_RS12040 0.55 +1.7 74,022 - EX31_RS12040 0.55 +0.1 74,022 - EX31_RS12040 0.55 -0.3 74,022 - EX31_RS12040 0.55 +0.6 74,022 - EX31_RS12040 0.55 -0.6 74,022 - EX31_RS12040 0.55 -1.4 74,022 - EX31_RS12040 0.55 +0.4 74,022 - EX31_RS12040 0.55 -0.4 74,053 + EX31_RS12040 0.59 -0.1 74,053 + EX31_RS12040 0.59 -1.4 74,054 - EX31_RS12040 0.59 -2.0 74,055 + EX31_RS12040 0.59 +0.5 74,055 + EX31_RS12040 0.59 +0.5 74,055 + EX31_RS12040 0.59 +1.7 74,055 + EX31_RS12040 0.59 +1.5 74,055 + EX31_RS12040 0.59 +1.7 74,055 + EX31_RS12040 0.59 +0.0 74,056 - EX31_RS12040 0.59 +0.6 74,056 - EX31_RS12040 0.59 +1.0 74,056 - EX31_RS12040 0.59 -1.6 74,056 - EX31_RS12040 0.59 +0.4 74,056 - EX31_RS12040 0.59 -1.1 74,067 + EX31_RS12040 0.61 +0.6 74,068 - EX31_RS12040 0.61 +0.2 74,068 - EX31_RS12040 0.61 +0.9 74,068 - EX31_RS12040 0.61 -0.6 74,068 - EX31_RS12040 0.61 +1.2 74,068 - EX31_RS12040 0.61 +0.6 74,076 + EX31_RS12040 0.62 -1.4 74,077 - EX31_RS12040 0.62 -1.8 74,077 - EX31_RS12040 0.62 -0.8 74,077 - EX31_RS12040 0.62 -0.0 74,077 - EX31_RS12040 0.62 -0.3 74,146 + EX31_RS12040 0.71 -0.4 74,156 + EX31_RS12040 0.73 +0.8 74,156 + EX31_RS12040 0.73 +0.7 74,157 - EX31_RS12040 0.73 -0.7 74,157 - EX31_RS12040 0.73 +1.6 74,157 - EX31_RS12040 0.73 +0.6 74,188 + EX31_RS12040 0.77 +0.0 74,189 - EX31_RS12040 0.77 +0.8 74,189 - EX31_RS12040 0.77 +1.0 74,352 - +1.5 74,355 + -0.0 74,355 + +1.0 74,355 + -0.5 74,375 + +0.9 74,376 - -1.1 74,452 + EX31_RS12045 0.22 -1.3 74,452 + EX31_RS12045 0.22 +0.2 74,452 + EX31_RS12045 0.22 +0.7 74,453 - EX31_RS12045 0.22 -2.7 74,492 + EX31_RS12045 0.33 +0.2 74,492 + EX31_RS12045 0.33 +0.9 74,492 + EX31_RS12045 0.33 +0.2 74,492 + EX31_RS12045 0.33 -0.4 74,493 - EX31_RS12045 0.33 +0.1 74,493 - EX31_RS12045 0.33 +0.4 74,493 - EX31_RS12045 0.33 +0.0
Or see this region's nucleotide sequence