Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10115 and EX31_RS10120 are separated by 213 nucleotides EX31_RS10120 and EX31_RS10125 are separated by 229 nucleotides
EX31_RS10115: EX31_RS10115 - formyltetrahydrofolate deformylase, at 233,062 to 233,910
_RS10115
EX31_RS10120: EX31_RS10120 - tRNA-Tyr, at 234,124 to 234,208
_RS10120
EX31_RS10125: EX31_RS10125 - ABC transporter ATP-binding protein, at 234,438 to 236,270
_RS10125
Position (kb) 234
235 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 233.142 kb on + strand at 233.163 kb on + strand, within EX31_RS10115 at 233.163 kb on + strand, within EX31_RS10115 at 233.163 kb on + strand, within EX31_RS10115 at 233.163 kb on + strand, within EX31_RS10115 at 233.164 kb on - strand, within EX31_RS10115 at 233.164 kb on - strand, within EX31_RS10115 at 233.166 kb on + strand, within EX31_RS10115 at 233.214 kb on + strand, within EX31_RS10115 at 233.463 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.554 kb on + strand, within EX31_RS10115 at 233.572 kb on + strand, within EX31_RS10115 at 233.572 kb on + strand, within EX31_RS10115 at 233.572 kb on + strand, within EX31_RS10115 at 233.573 kb on - strand, within EX31_RS10115 at 233.573 kb on - strand, within EX31_RS10115 at 233.582 kb on + strand, within EX31_RS10115 at 233.609 kb on - strand, within EX31_RS10115 at 233.656 kb on - strand, within EX31_RS10115 at 233.683 kb on + strand, within EX31_RS10115 at 233.723 kb on - strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.784 kb on + strand, within EX31_RS10115 at 233.785 kb on - strand, within EX31_RS10115 at 233.785 kb on - strand, within EX31_RS10115 at 233.785 kb on - strand, within EX31_RS10115 at 233.788 kb on + strand, within EX31_RS10115 at 233.788 kb on + strand, within EX31_RS10115 at 233.788 kb on + strand, within EX31_RS10115 at 233.788 kb on + strand, within EX31_RS10115 at 233.789 kb on - strand, within EX31_RS10115 at 233.789 kb on - strand, within EX31_RS10115 at 233.789 kb on - strand, within EX31_RS10115 at 233.789 kb on - strand, within EX31_RS10115 at 233.789 kb on - strand, within EX31_RS10115 at 233.790 kb on + strand, within EX31_RS10115 at 233.790 kb on + strand, within EX31_RS10115 at 233.790 kb on + strand, within EX31_RS10115 at 233.791 kb on - strand, within EX31_RS10115 at 233.791 kb on - strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.802 kb on + strand, within EX31_RS10115 at 233.852 kb on + strand at 233.853 kb on - strand at 233.853 kb on - strand at 233.853 kb on - strand at 233.854 kb on + strand at 233.854 kb on + strand at 233.854 kb on + strand at 233.884 kb on + strand at 233.908 kb on + strand at 234.020 kb on - strand at 234.041 kb on + strand at 234.071 kb on + strand at 234.071 kb on + strand at 234.071 kb on + strand at 234.071 kb on + strand at 234.071 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.098 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.102 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.105 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.214 kb on + strand at 234.248 kb on + strand at 234.248 kb on + strand at 234.252 kb on + strand at 234.362 kb on + strand at 234.414 kb on - strand at 234.426 kb on + strand at 234.432 kb on + strand at 234.432 kb on + strand at 234.495 kb on - strand at 234.495 kb on - strand at 234.496 kb on + strand at 234.496 kb on + strand at 234.549 kb on - strand at 234.604 kb on + strand at 234.628 kb on - strand, within EX31_RS10125 at 234.668 kb on - strand, within EX31_RS10125 at 234.668 kb on - strand, within EX31_RS10125 at 234.723 kb on - strand, within EX31_RS10125 at 234.861 kb on - strand, within EX31_RS10125 at 234.986 kb on + strand, within EX31_RS10125 at 234.986 kb on + strand, within EX31_RS10125 at 234.987 kb on - strand, within EX31_RS10125 at 234.987 kb on - strand, within EX31_RS10125 at 235.028 kb on + strand, within EX31_RS10125
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 233,142 + -1.9 233,163 + EX31_RS10115 0.12 -1.0 233,163 + EX31_RS10115 0.12 +0.5 233,163 + EX31_RS10115 0.12 -0.3 233,163 + EX31_RS10115 0.12 +0.6 233,164 - EX31_RS10115 0.12 -0.2 233,164 - EX31_RS10115 0.12 +0.0 233,166 + EX31_RS10115 0.12 -2.5 233,214 + EX31_RS10115 0.18 -2.9 233,463 + EX31_RS10115 0.47 -3.3 233,554 + EX31_RS10115 0.58 -0.0 233,554 + EX31_RS10115 0.58 -1.2 233,554 + EX31_RS10115 0.58 -0.5 233,554 + EX31_RS10115 0.58 -0.6 233,554 + EX31_RS10115 0.58 -1.7 233,554 + EX31_RS10115 0.58 -0.4 233,572 + EX31_RS10115 0.60 +0.0 233,572 + EX31_RS10115 0.60 -1.4 233,572 + EX31_RS10115 0.60 -2.2 233,573 - EX31_RS10115 0.60 +0.3 233,573 - EX31_RS10115 0.60 -2.4 233,582 + EX31_RS10115 0.61 -3.0 233,609 - EX31_RS10115 0.64 -1.3 233,656 - EX31_RS10115 0.70 -2.8 233,683 + EX31_RS10115 0.73 -0.7 233,723 - EX31_RS10115 0.78 +0.8 233,784 + EX31_RS10115 0.85 -3.1 233,784 + EX31_RS10115 0.85 -0.8 233,784 + EX31_RS10115 0.85 -3.0 233,784 + EX31_RS10115 0.85 -2.9 233,784 + EX31_RS10115 0.85 -3.1 233,784 + EX31_RS10115 0.85 -0.1 233,784 + EX31_RS10115 0.85 -2.3 233,784 + EX31_RS10115 0.85 -0.3 233,784 + EX31_RS10115 0.85 +0.4 233,785 - EX31_RS10115 0.85 +0.6 233,785 - EX31_RS10115 0.85 +0.4 233,785 - EX31_RS10115 0.85 -0.8 233,788 + EX31_RS10115 0.86 +1.8 233,788 + EX31_RS10115 0.86 -2.5 233,788 + EX31_RS10115 0.86 -2.7 233,788 + EX31_RS10115 0.86 -1.8 233,789 - EX31_RS10115 0.86 -1.5 233,789 - EX31_RS10115 0.86 -1.0 233,789 - EX31_RS10115 0.86 +1.6 233,789 - EX31_RS10115 0.86 -0.7 233,789 - EX31_RS10115 0.86 -1.5 233,790 + EX31_RS10115 0.86 -3.0 233,790 + EX31_RS10115 0.86 -2.4 233,790 + EX31_RS10115 0.86 -0.0 233,791 - EX31_RS10115 0.86 -0.5 233,791 - EX31_RS10115 0.86 -1.2 233,802 + EX31_RS10115 0.87 +0.0 233,802 + EX31_RS10115 0.87 +0.6 233,802 + EX31_RS10115 0.87 -2.7 233,802 + EX31_RS10115 0.87 -0.1 233,802 + EX31_RS10115 0.87 -0.4 233,802 + EX31_RS10115 0.87 -1.2 233,802 + EX31_RS10115 0.87 +1.6 233,852 + -1.1 233,853 - -1.5 233,853 - +0.4 233,853 - -1.0 233,854 + -1.4 233,854 + -1.6 233,854 + -1.7 233,884 + +0.3 233,908 + -2.1 234,020 - -1.0 234,041 + -0.1 234,071 + -0.2 234,071 + +0.4 234,071 + +0.0 234,071 + +2.6 234,071 + -0.6 234,098 + -0.4 234,098 + +0.0 234,098 + +0.0 234,098 + +0.4 234,098 + +0.8 234,098 + -0.1 234,098 + +0.4 234,098 + -0.5 234,098 + -0.2 234,098 + -0.4 234,098 + -0.8 234,098 + -0.2 234,102 + -0.3 234,102 + +0.7 234,102 + -0.5 234,102 + +0.2 234,102 + -0.2 234,102 + +0.2 234,102 + -0.3 234,102 + -0.1 234,105 + +1.1 234,105 + -0.4 234,105 + -0.4 234,105 + +1.6 234,105 + +0.9 234,105 + -0.1 234,105 + -0.3 234,214 + -1.4 234,214 + +0.5 234,214 + -0.4 234,214 + -0.3 234,214 + +0.9 234,214 + +0.6 234,214 + -0.1 234,214 + +0.5 234,214 + -1.8 234,214 + -0.5 234,248 + +0.6 234,248 + -0.4 234,252 + -0.4 234,362 + +0.9 234,414 - +2.1 234,426 + -0.9 234,432 + +1.2 234,432 + +1.3 234,495 - -1.5 234,495 - +0.7 234,496 + -1.2 234,496 + +1.8 234,549 - -0.2 234,604 + -1.0 234,628 - EX31_RS10125 0.10 +1.2 234,668 - EX31_RS10125 0.13 -1.0 234,668 - EX31_RS10125 0.13 +0.0 234,723 - EX31_RS10125 0.16 +1.1 234,861 - EX31_RS10125 0.23 +0.2 234,986 + EX31_RS10125 0.30 +0.4 234,986 + EX31_RS10125 0.30 -1.1 234,987 - EX31_RS10125 0.30 +0.6 234,987 - EX31_RS10125 0.30 -0.8 235,028 + EX31_RS10125 0.32 +1.2
Or see this region's nucleotide sequence