Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09955 and EX31_RS09960 are separated by 1 nucleotides EX31_RS09960 and EX31_RS09965 are separated by 11 nucleotides EX31_RS09965 and EX31_RS09970 are separated by 3 nucleotides
EX31_RS09955: EX31_RS09955 - anthranilate synthase component 1, at 199,984 to 201,546
_RS09955
EX31_RS09960: EX31_RS09960 - glutamine amidotransferase, at 201,548 to 202,126
_RS09960
EX31_RS09965: EX31_RS09965 - anthranilate phosphoribosyltransferase, at 202,138 to 203,151
_RS09965
EX31_RS09970: EX31_RS09970 - bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF, at 203,155 to 204,519
_RS09970
Position (kb) 202
203
204 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 201.147 kb on + strand, within EX31_RS09955 at 201.222 kb on - strand, within EX31_RS09955 at 201.222 kb on - strand, within EX31_RS09955 at 201.222 kb on - strand, within EX31_RS09955 at 201.232 kb on + strand, within EX31_RS09955 at 201.233 kb on - strand, within EX31_RS09955 at 201.346 kb on + strand, within EX31_RS09955 at 201.347 kb on - strand, within EX31_RS09955 at 201.368 kb on + strand, within EX31_RS09955 at 201.368 kb on + strand, within EX31_RS09955 at 201.372 kb on + strand, within EX31_RS09955 at 201.398 kb on - strand at 201.398 kb on - strand at 201.398 kb on - strand at 201.483 kb on + strand at 201.486 kb on + strand at 201.486 kb on + strand at 201.540 kb on - strand at 201.544 kb on + strand at 201.544 kb on + strand at 201.545 kb on - strand at 201.545 kb on - strand at 201.562 kb on - strand at 201.562 kb on - strand at 201.562 kb on - strand at 201.562 kb on - strand at 201.587 kb on - strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.590 kb on + strand at 201.591 kb on - strand at 201.591 kb on - strand at 201.591 kb on - strand at 201.591 kb on - strand at 201.591 kb on - strand at 201.625 kb on + strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.626 kb on - strand, within EX31_RS09960 at 201.638 kb on + strand, within EX31_RS09960 at 201.827 kb on + strand, within EX31_RS09960 at 201.880 kb on + strand, within EX31_RS09960 at 201.880 kb on + strand, within EX31_RS09960 at 201.880 kb on + strand, within EX31_RS09960 at 201.880 kb on + strand, within EX31_RS09960 at 201.881 kb on - strand, within EX31_RS09960 at 201.883 kb on + strand, within EX31_RS09960 at 201.883 kb on + strand, within EX31_RS09960 at 202.004 kb on - strand, within EX31_RS09960 at 202.017 kb on + strand, within EX31_RS09960 at 202.018 kb on - strand, within EX31_RS09960 at 202.152 kb on + strand at 202.153 kb on - strand at 202.217 kb on + strand at 202.218 kb on - strand at 202.218 kb on - strand at 202.419 kb on + strand, within EX31_RS09965 at 202.419 kb on + strand, within EX31_RS09965 at 202.420 kb on - strand, within EX31_RS09965 at 202.518 kb on - strand, within EX31_RS09965 at 202.647 kb on + strand, within EX31_RS09965 at 202.732 kb on + strand, within EX31_RS09965 at 202.783 kb on + strand, within EX31_RS09965 at 202.874 kb on + strand, within EX31_RS09965 at 202.882 kb on + strand, within EX31_RS09965 at 202.882 kb on + strand, within EX31_RS09965 at 202.882 kb on + strand, within EX31_RS09965 at 202.914 kb on + strand, within EX31_RS09965 at 202.914 kb on + strand, within EX31_RS09965 at 202.918 kb on + strand, within EX31_RS09965 at 202.918 kb on + strand, within EX31_RS09965 at 202.926 kb on - strand, within EX31_RS09965 at 202.979 kb on + strand, within EX31_RS09965 at 202.982 kb on + strand, within EX31_RS09965 at 202.982 kb on + strand, within EX31_RS09965 at 202.982 kb on + strand, within EX31_RS09965 at 202.982 kb on + strand, within EX31_RS09965 at 202.982 kb on + strand, within EX31_RS09965 at 202.982 kb on + strand, within EX31_RS09965 at 202.982 kb on + strand, within EX31_RS09965 at 202.982 kb on + strand, within EX31_RS09965 at 202.983 kb on - strand, within EX31_RS09965 at 202.983 kb on - strand, within EX31_RS09965 at 202.983 kb on - strand, within EX31_RS09965 at 202.983 kb on - strand, within EX31_RS09965 at 202.989 kb on + strand, within EX31_RS09965 at 203.116 kb on + strand at 203.150 kb on - strand at 203.218 kb on - strand at 203.255 kb on + strand at 203.255 kb on + strand at 203.255 kb on + strand at 203.255 kb on + strand at 203.255 kb on + strand at 203.256 kb on - strand at 203.256 kb on - strand at 203.256 kb on - strand at 203.278 kb on + strand at 203.278 kb on + strand at 203.278 kb on + strand at 203.278 kb on + strand at 203.399 kb on - strand, within EX31_RS09970 at 203.399 kb on - strand, within EX31_RS09970 at 203.420 kb on + strand, within EX31_RS09970 at 203.434 kb on + strand, within EX31_RS09970 at 203.522 kb on - strand, within EX31_RS09970 at 203.596 kb on + strand, within EX31_RS09970 at 203.596 kb on + strand, within EX31_RS09970 at 203.596 kb on + strand, within EX31_RS09970 at 203.596 kb on + strand, within EX31_RS09970 at 203.596 kb on + strand, within EX31_RS09970 at 203.597 kb on - strand, within EX31_RS09970 at 203.628 kb on + strand, within EX31_RS09970 at 203.811 kb on + strand, within EX31_RS09970 at 203.811 kb on + strand, within EX31_RS09970 at 203.811 kb on + strand, within EX31_RS09970 at 203.811 kb on + strand, within EX31_RS09970 at 203.812 kb on - strand, within EX31_RS09970 at 203.814 kb on + strand, within EX31_RS09970 at 203.814 kb on + strand, within EX31_RS09970 at 203.814 kb on + strand, within EX31_RS09970 at 203.814 kb on + strand, within EX31_RS09970 at 203.815 kb on - strand, within EX31_RS09970 at 204.079 kb on + strand, within EX31_RS09970 at 204.079 kb on + strand, within EX31_RS09970 at 204.079 kb on + strand, within EX31_RS09970 at 204.079 kb on + strand, within EX31_RS09970 at 204.080 kb on - strand, within EX31_RS09970 at 204.080 kb on - strand, within EX31_RS09970 at 204.080 kb on - strand, within EX31_RS09970 at 204.080 kb on - strand, within EX31_RS09970 at 204.137 kb on + strand, within EX31_RS09970 at 204.137 kb on + strand, within EX31_RS09970
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 201,147 + EX31_RS09955 0.74 -0.7 201,222 - EX31_RS09955 0.79 -2.1 201,222 - EX31_RS09955 0.79 +0.0 201,222 - EX31_RS09955 0.79 -2.1 201,232 + EX31_RS09955 0.80 -2.8 201,233 - EX31_RS09955 0.80 -0.8 201,346 + EX31_RS09955 0.87 -2.1 201,347 - EX31_RS09955 0.87 -1.7 201,368 + EX31_RS09955 0.89 -1.0 201,368 + EX31_RS09955 0.89 -2.1 201,372 + EX31_RS09955 0.89 -1.0 201,398 - -1.4 201,398 - -1.3 201,398 - -2.4 201,483 + -2.5 201,486 + +0.6 201,486 + -2.2 201,540 - +0.6 201,544 + -2.0 201,544 + -1.4 201,545 - +1.2 201,545 - -0.7 201,562 - -2.3 201,562 - -0.7 201,562 - -2.0 201,562 - -2.5 201,587 - -1.8 201,590 + -2.6 201,590 + -3.8 201,590 + -1.2 201,590 + -1.8 201,590 + -1.9 201,590 + -2.3 201,590 + -2.4 201,590 + -3.8 201,590 + -2.8 201,590 + -0.4 201,590 + -1.4 201,591 - -0.9 201,591 - -2.8 201,591 - -0.1 201,591 - -3.1 201,591 - -2.5 201,625 + EX31_RS09960 0.13 -0.2 201,626 - EX31_RS09960 0.13 +0.6 201,626 - EX31_RS09960 0.13 -0.4 201,626 - EX31_RS09960 0.13 -2.6 201,626 - EX31_RS09960 0.13 -1.5 201,626 - EX31_RS09960 0.13 -1.4 201,626 - EX31_RS09960 0.13 -0.4 201,638 + EX31_RS09960 0.16 -1.8 201,827 + EX31_RS09960 0.48 -1.0 201,880 + EX31_RS09960 0.57 -1.2 201,880 + EX31_RS09960 0.57 -1.8 201,880 + EX31_RS09960 0.57 -1.5 201,880 + EX31_RS09960 0.57 -1.4 201,881 - EX31_RS09960 0.58 -1.5 201,883 + EX31_RS09960 0.58 -3.2 201,883 + EX31_RS09960 0.58 -2.5 202,004 - EX31_RS09960 0.79 -1.2 202,017 + EX31_RS09960 0.81 -1.0 202,018 - EX31_RS09960 0.81 -3.5 202,152 + -2.2 202,153 - -1.3 202,217 + -0.8 202,218 - +0.5 202,218 - -1.7 202,419 + EX31_RS09965 0.28 -2.3 202,419 + EX31_RS09965 0.28 -2.7 202,420 - EX31_RS09965 0.28 +1.6 202,518 - EX31_RS09965 0.37 -2.4 202,647 + EX31_RS09965 0.50 -2.4 202,732 + EX31_RS09965 0.59 -3.8 202,783 + EX31_RS09965 0.64 -1.5 202,874 + EX31_RS09965 0.73 -2.0 202,882 + EX31_RS09965 0.73 -2.5 202,882 + EX31_RS09965 0.73 -1.7 202,882 + EX31_RS09965 0.73 -2.8 202,914 + EX31_RS09965 0.77 -3.1 202,914 + EX31_RS09965 0.77 -0.7 202,918 + EX31_RS09965 0.77 -2.0 202,918 + EX31_RS09965 0.77 -3.0 202,926 - EX31_RS09965 0.78 -2.0 202,979 + EX31_RS09965 0.83 -1.2 202,982 + EX31_RS09965 0.83 +0.0 202,982 + EX31_RS09965 0.83 -2.8 202,982 + EX31_RS09965 0.83 -2.2 202,982 + EX31_RS09965 0.83 -2.0 202,982 + EX31_RS09965 0.83 -2.3 202,982 + EX31_RS09965 0.83 -1.8 202,982 + EX31_RS09965 0.83 -3.0 202,982 + EX31_RS09965 0.83 -1.0 202,983 - EX31_RS09965 0.83 -1.7 202,983 - EX31_RS09965 0.83 -1.2 202,983 - EX31_RS09965 0.83 -1.2 202,983 - EX31_RS09965 0.83 -2.8 202,989 + EX31_RS09965 0.84 -1.5 203,116 + -2.5 203,150 - -2.1 203,218 - -1.0 203,255 + -2.4 203,255 + -0.4 203,255 + -0.4 203,255 + +1.6 203,255 + -2.3 203,256 - -0.4 203,256 - -3.0 203,256 - -3.1 203,278 + -2.0 203,278 + -1.0 203,278 + -2.5 203,278 + +0.0 203,399 - EX31_RS09970 0.18 -0.8 203,399 - EX31_RS09970 0.18 +0.6 203,420 + EX31_RS09970 0.19 -1.0 203,434 + EX31_RS09970 0.20 -1.7 203,522 - EX31_RS09970 0.27 -2.3 203,596 + EX31_RS09970 0.32 -0.4 203,596 + EX31_RS09970 0.32 -1.8 203,596 + EX31_RS09970 0.32 -0.8 203,596 + EX31_RS09970 0.32 -2.0 203,596 + EX31_RS09970 0.32 -1.8 203,597 - EX31_RS09970 0.32 -0.9 203,628 + EX31_RS09970 0.35 -1.3 203,811 + EX31_RS09970 0.48 -1.5 203,811 + EX31_RS09970 0.48 -1.4 203,811 + EX31_RS09970 0.48 -1.5 203,811 + EX31_RS09970 0.48 -2.6 203,812 - EX31_RS09970 0.48 -2.0 203,814 + EX31_RS09970 0.48 -2.6 203,814 + EX31_RS09970 0.48 -2.7 203,814 + EX31_RS09970 0.48 -2.4 203,814 + EX31_RS09970 0.48 -0.7 203,815 - EX31_RS09970 0.48 -2.4 204,079 + EX31_RS09970 0.68 -1.4 204,079 + EX31_RS09970 0.68 -1.2 204,079 + EX31_RS09970 0.68 -2.8 204,079 + EX31_RS09970 0.68 -2.2 204,080 - EX31_RS09970 0.68 -1.7 204,080 - EX31_RS09970 0.68 -1.8 204,080 - EX31_RS09970 0.68 -2.6 204,080 - EX31_RS09970 0.68 -1.5 204,137 + EX31_RS09970 0.72 -3.8 204,137 + EX31_RS09970 0.72 -3.0
Or see this region's nucleotide sequence