Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09635 and EX31_RS09640 are separated by 148 nucleotides EX31_RS09640 and EX31_RS09645 are separated by 18 nucleotides
EX31_RS09635: EX31_RS09635 - YbjN domain-containing protein, at 129,850 to 130,332
_RS09635
EX31_RS09640: EX31_RS09640 - 30S ribosomal protein S6--L-glutamate ligase, at 130,481 to 131,383
_RS09640
EX31_RS09645: EX31_RS09645 - oxygen-insensitive NADPH nitroreductase, at 131,402 to 132,124
_RS09645
Position (kb) 130
131
132 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 129.516 kb on - strand at 129.516 kb on - strand at 129.522 kb on - strand at 129.522 kb on - strand at 129.579 kb on + strand at 129.579 kb on + strand at 129.609 kb on - strand at 129.629 kb on - strand at 129.683 kb on + strand at 129.683 kb on + strand at 129.684 kb on - strand at 129.708 kb on - strand at 129.720 kb on + strand at 129.721 kb on - strand at 129.749 kb on + strand at 129.860 kb on - strand at 129.860 kb on - strand at 129.942 kb on + strand, within EX31_RS09635 at 129.943 kb on - strand, within EX31_RS09635 at 129.943 kb on - strand, within EX31_RS09635 at 130.077 kb on + strand, within EX31_RS09635 at 130.176 kb on + strand, within EX31_RS09635 at 130.177 kb on - strand, within EX31_RS09635 at 130.177 kb on - strand, within EX31_RS09635 at 130.180 kb on + strand, within EX31_RS09635 at 130.181 kb on - strand, within EX31_RS09635 at 130.181 kb on - strand, within EX31_RS09635 at 130.181 kb on - strand, within EX31_RS09635 at 130.181 kb on - strand, within EX31_RS09635 at 130.181 kb on - strand, within EX31_RS09635 at 130.181 kb on - strand, within EX31_RS09635 at 130.225 kb on - strand, within EX31_RS09635 at 130.306 kb on + strand at 130.306 kb on + strand at 130.307 kb on - strand at 130.333 kb on - strand at 130.333 kb on - strand at 130.333 kb on - strand at 130.394 kb on - strand at 130.394 kb on - strand at 130.406 kb on + strand at 130.406 kb on + strand at 130.406 kb on + strand at 130.406 kb on + strand at 130.406 kb on + strand at 130.407 kb on - strand at 130.407 kb on - strand at 130.407 kb on - strand at 130.419 kb on - strand at 130.435 kb on - strand at 130.462 kb on + strand at 130.462 kb on + strand at 130.529 kb on - strand at 130.529 kb on - strand at 130.530 kb on + strand at 130.531 kb on - strand at 130.601 kb on - strand, within EX31_RS09640 at 130.634 kb on + strand, within EX31_RS09640 at 130.848 kb on + strand, within EX31_RS09640 at 130.848 kb on + strand, within EX31_RS09640 at 130.849 kb on - strand, within EX31_RS09640 at 130.849 kb on - strand, within EX31_RS09640 at 130.849 kb on - strand, within EX31_RS09640 at 130.849 kb on - strand, within EX31_RS09640 at 130.849 kb on - strand, within EX31_RS09640 at 130.849 kb on - strand, within EX31_RS09640 at 131.024 kb on - strand, within EX31_RS09640 at 131.170 kb on - strand, within EX31_RS09640 at 131.211 kb on + strand, within EX31_RS09640 at 131.212 kb on - strand, within EX31_RS09640 at 131.212 kb on - strand, within EX31_RS09640 at 131.212 kb on - strand, within EX31_RS09640 at 131.212 kb on - strand, within EX31_RS09640 at 131.212 kb on - strand, within EX31_RS09640 at 131.212 kb on - strand, within EX31_RS09640 at 131.235 kb on + strand, within EX31_RS09640 at 131.263 kb on + strand, within EX31_RS09640 at 131.272 kb on - strand, within EX31_RS09640 at 131.272 kb on - strand, within EX31_RS09640 at 131.291 kb on - strand, within EX31_RS09640 at 131.291 kb on - strand, within EX31_RS09640 at 131.291 kb on - strand, within EX31_RS09640 at 131.346 kb on + strand at 131.347 kb on - strand at 131.347 kb on - strand at 131.432 kb on + strand at 131.443 kb on + strand at 131.444 kb on - strand at 131.444 kb on - strand at 131.464 kb on + strand at 131.477 kb on - strand, within EX31_RS09645 at 131.527 kb on - strand, within EX31_RS09645 at 131.527 kb on - strand, within EX31_RS09645 at 131.529 kb on + strand, within EX31_RS09645 at 131.529 kb on + strand, within EX31_RS09645 at 131.530 kb on - strand, within EX31_RS09645 at 131.550 kb on + strand, within EX31_RS09645 at 131.570 kb on - strand, within EX31_RS09645 at 131.570 kb on - strand, within EX31_RS09645 at 131.570 kb on - strand, within EX31_RS09645 at 131.573 kb on + strand, within EX31_RS09645 at 131.574 kb on - strand, within EX31_RS09645 at 131.583 kb on + strand, within EX31_RS09645 at 131.584 kb on - strand, within EX31_RS09645 at 131.617 kb on + strand, within EX31_RS09645 at 131.657 kb on - strand, within EX31_RS09645 at 131.657 kb on - strand, within EX31_RS09645 at 131.660 kb on + strand, within EX31_RS09645 at 131.661 kb on - strand, within EX31_RS09645 at 131.661 kb on - strand, within EX31_RS09645 at 131.661 kb on - strand, within EX31_RS09645 at 131.661 kb on - strand, within EX31_RS09645 at 131.709 kb on - strand, within EX31_RS09645 at 131.865 kb on - strand, within EX31_RS09645 at 131.920 kb on - strand, within EX31_RS09645 at 131.920 kb on - strand, within EX31_RS09645 at 132.149 kb on + strand at 132.149 kb on + strand at 132.149 kb on + strand at 132.149 kb on + strand at 132.150 kb on - strand at 132.150 kb on - strand at 132.150 kb on - strand at 132.153 kb on + strand at 132.213 kb on + strand at 132.213 kb on + strand at 132.213 kb on + strand at 132.213 kb on + strand at 132.214 kb on - strand at 132.288 kb on + strand at 132.288 kb on + strand at 132.323 kb on + strand at 132.323 kb on + strand at 132.324 kb on - strand at 132.324 kb on - strand at 132.326 kb on + strand at 132.380 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 129,516 - -1.6 129,516 - +2.6 129,522 - +1.1 129,522 - +0.9 129,579 + +0.8 129,579 + +0.9 129,609 - +2.6 129,629 - +1.0 129,683 + -0.3 129,683 + -0.4 129,684 - -1.7 129,708 - +1.6 129,720 + -0.3 129,721 - +1.6 129,749 + +0.4 129,860 - +0.0 129,860 - +1.4 129,942 + EX31_RS09635 0.19 +1.6 129,943 - EX31_RS09635 0.19 +0.6 129,943 - EX31_RS09635 0.19 +0.6 130,077 + EX31_RS09635 0.47 +0.4 130,176 + EX31_RS09635 0.67 -0.3 130,177 - EX31_RS09635 0.68 -1.4 130,177 - EX31_RS09635 0.68 +0.8 130,180 + EX31_RS09635 0.68 +1.6 130,181 - EX31_RS09635 0.69 -1.5 130,181 - EX31_RS09635 0.69 +0.2 130,181 - EX31_RS09635 0.69 -2.0 130,181 - EX31_RS09635 0.69 +0.2 130,181 - EX31_RS09635 0.69 +0.4 130,181 - EX31_RS09635 0.69 +1.0 130,225 - EX31_RS09635 0.78 +0.9 130,306 + +0.0 130,306 + +0.4 130,307 - +0.3 130,333 - +1.0 130,333 - +0.6 130,333 - -0.1 130,394 - +0.9 130,394 - +1.0 130,406 + -0.1 130,406 + +0.5 130,406 + -0.4 130,406 + +1.4 130,406 + +0.4 130,407 - +0.4 130,407 - -0.4 130,407 - -2.5 130,419 - +1.9 130,435 - -0.6 130,462 + +1.3 130,462 + +1.2 130,529 - +1.6 130,529 - +0.0 130,530 + +1.1 130,531 - +2.6 130,601 - EX31_RS09640 0.13 -1.3 130,634 + EX31_RS09640 0.17 -1.0 130,848 + EX31_RS09640 0.41 -0.8 130,848 + EX31_RS09640 0.41 -0.7 130,849 - EX31_RS09640 0.41 +1.6 130,849 - EX31_RS09640 0.41 -1.4 130,849 - EX31_RS09640 0.41 -0.4 130,849 - EX31_RS09640 0.41 +0.6 130,849 - EX31_RS09640 0.41 -2.5 130,849 - EX31_RS09640 0.41 +0.0 131,024 - EX31_RS09640 0.60 -0.3 131,170 - EX31_RS09640 0.76 -2.9 131,211 + EX31_RS09640 0.81 +0.2 131,212 - EX31_RS09640 0.81 +0.8 131,212 - EX31_RS09640 0.81 +0.5 131,212 - EX31_RS09640 0.81 -0.1 131,212 - EX31_RS09640 0.81 -1.8 131,212 - EX31_RS09640 0.81 -0.3 131,212 - EX31_RS09640 0.81 +2.6 131,235 + EX31_RS09640 0.83 +0.6 131,263 + EX31_RS09640 0.87 +0.9 131,272 - EX31_RS09640 0.88 -1.4 131,272 - EX31_RS09640 0.88 +0.8 131,291 - EX31_RS09640 0.90 -0.7 131,291 - EX31_RS09640 0.90 -1.2 131,291 - EX31_RS09640 0.90 -0.5 131,346 + +0.6 131,347 - +0.0 131,347 - +0.6 131,432 + +1.4 131,443 + -1.6 131,444 - -0.8 131,444 - -0.4 131,464 + +1.5 131,477 - EX31_RS09645 0.10 +1.4 131,527 - EX31_RS09645 0.17 -1.4 131,527 - EX31_RS09645 0.17 +0.8 131,529 + EX31_RS09645 0.18 +0.2 131,529 + EX31_RS09645 0.18 +1.1 131,530 - EX31_RS09645 0.18 +1.0 131,550 + EX31_RS09645 0.20 +0.7 131,570 - EX31_RS09645 0.23 -0.7 131,570 - EX31_RS09645 0.23 +1.2 131,570 - EX31_RS09645 0.23 +2.3 131,573 + EX31_RS09645 0.24 +0.0 131,574 - EX31_RS09645 0.24 +0.6 131,583 + EX31_RS09645 0.25 -1.8 131,584 - EX31_RS09645 0.25 +0.1 131,617 + EX31_RS09645 0.30 -0.7 131,657 - EX31_RS09645 0.35 +2.2 131,657 - EX31_RS09645 0.35 -1.1 131,660 + EX31_RS09645 0.36 +2.2 131,661 - EX31_RS09645 0.36 +0.6 131,661 - EX31_RS09645 0.36 -0.5 131,661 - EX31_RS09645 0.36 +0.3 131,661 - EX31_RS09645 0.36 -1.8 131,709 - EX31_RS09645 0.42 -0.6 131,865 - EX31_RS09645 0.64 -1.0 131,920 - EX31_RS09645 0.72 +0.1 131,920 - EX31_RS09645 0.72 -0.6 132,149 + -2.0 132,149 + +2.8 132,149 + +0.0 132,149 + +0.4 132,150 - +0.2 132,150 - +0.7 132,150 - +0.5 132,153 + -1.2 132,213 + +0.6 132,213 + +0.5 132,213 + +0.4 132,213 + +0.2 132,214 - +1.0 132,288 + -1.6 132,288 + +0.6 132,323 + -2.2 132,323 + +0.7 132,324 - -0.2 132,324 - +0.5 132,326 + +0.1 132,380 + -0.6
Or see this region's nucleotide sequence