Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03850 and EX31_RS03855 are separated by 65 nucleotides EX31_RS03855 and EX31_RS03860 overlap by 20 nucleotides EX31_RS03860 and EX31_RS03865 are separated by 5 nucleotides EX31_RS03865 and EX31_RS03870 overlap by 1 nucleotides
EX31_RS03850: EX31_RS03850 - SMP-30/gluconolactonase/LRE family protein, at 283,252 to 284,127
_RS03850
EX31_RS03855: EX31_RS03855 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 284,193 to 285,146
_RS03855
EX31_RS03860: EX31_RS03860 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 285,127 to 285,597
_RS03860
EX31_RS03865: EX31_RS03865 - lipoprotein signal peptidase, at 285,603 to 286,109
_RS03865
EX31_RS03870: EX31_RS03870 - isoleucine--tRNA ligase, at 286,109 to 288,925
_RS03870
Position (kb) 285
286 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 284.147 kb on + strand at 284.181 kb on + strand at 284.182 kb on - strand at 284.182 kb on - strand at 285.199 kb on - strand, within EX31_RS03860 at 285.428 kb on - strand, within EX31_RS03860 at 285.440 kb on - strand, within EX31_RS03860 at 285.440 kb on - strand, within EX31_RS03860 at 285.440 kb on - strand, within EX31_RS03860 at 285.553 kb on - strand at 285.553 kb on - strand at 285.553 kb on - strand at 285.553 kb on - strand at 285.566 kb on - strand at 285.566 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.598 kb on - strand at 285.621 kb on - strand at 285.621 kb on - strand at 285.621 kb on - strand at 285.621 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 284,147 + +0.2 284,181 + +2.6 284,182 - +0.2 284,182 - -0.2 285,199 - EX31_RS03860 0.15 -3.4 285,428 - EX31_RS03860 0.64 +0.7 285,440 - EX31_RS03860 0.66 -0.5 285,440 - EX31_RS03860 0.66 -0.6 285,440 - EX31_RS03860 0.66 -1.4 285,553 - -1.0 285,553 - +1.5 285,553 - -0.6 285,553 - +0.6 285,566 - +0.3 285,566 - -0.5 285,598 - +1.6 285,598 - -0.1 285,598 - +1.0 285,598 - +1.3 285,598 - -0.3 285,598 - -0.1 285,598 - +0.8 285,598 - -0.1 285,598 - +0.7 285,598 - -1.2 285,598 - +0.3 285,598 - +1.2 285,598 - -1.0 285,598 - -0.8 285,598 - +2.3 285,598 - -0.6 285,598 - -0.8 285,598 - +0.3 285,598 - -1.6 285,598 - +0.9 285,621 - +0.3 285,621 - -3.0 285,621 - +1.4 285,621 - +0.7
Or see this region's nucleotide sequence