Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03030 and EX31_RS03035 overlap by 14 nucleotides EX31_RS03035 and EX31_RS03040 overlap by 4 nucleotides
EX31_RS03030: EX31_RS03030 - helix-turn-helix transcriptional regulator, at 88,178 to 88,984
_RS03030
EX31_RS03035: EX31_RS03035 - LysE family transporter, at 88,971 to 89,609
_RS03035
EX31_RS03040: EX31_RS03040 - hypothetical protein, at 89,606 to 90,898
_RS03040
Position (kb) 88
89
90 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 87.981 kb on - strand at 87.983 kb on + strand at 87.983 kb on + strand at 87.984 kb on - strand at 87.996 kb on + strand at 87.996 kb on + strand at 87.996 kb on + strand at 87.996 kb on + strand at 87.996 kb on + strand at 87.996 kb on + strand at 87.997 kb on - strand at 87.997 kb on - strand at 88.032 kb on + strand at 88.111 kb on - strand at 88.121 kb on + strand at 88.123 kb on + strand at 88.123 kb on + strand at 88.123 kb on + strand at 88.123 kb on + strand at 88.123 kb on + strand at 88.124 kb on - strand at 88.138 kb on + strand at 88.139 kb on - strand at 88.139 kb on - strand at 88.190 kb on - strand at 88.190 kb on - strand at 88.190 kb on - strand at 88.210 kb on + strand at 88.264 kb on + strand, within EX31_RS03030 at 88.264 kb on + strand, within EX31_RS03030 at 88.264 kb on + strand, within EX31_RS03030 at 88.264 kb on + strand, within EX31_RS03030 at 88.264 kb on + strand, within EX31_RS03030 at 88.265 kb on - strand, within EX31_RS03030 at 88.265 kb on - strand, within EX31_RS03030 at 88.265 kb on - strand, within EX31_RS03030 at 88.265 kb on - strand, within EX31_RS03030 at 88.277 kb on + strand, within EX31_RS03030 at 88.277 kb on + strand, within EX31_RS03030 at 88.277 kb on + strand, within EX31_RS03030 at 88.277 kb on + strand, within EX31_RS03030 at 88.277 kb on + strand, within EX31_RS03030 at 88.277 kb on + strand, within EX31_RS03030 at 88.277 kb on + strand, within EX31_RS03030 at 88.277 kb on + strand, within EX31_RS03030 at 88.278 kb on - strand, within EX31_RS03030 at 88.278 kb on - strand, within EX31_RS03030 at 88.278 kb on - strand, within EX31_RS03030 at 88.278 kb on - strand, within EX31_RS03030 at 88.354 kb on + strand, within EX31_RS03030 at 88.364 kb on + strand, within EX31_RS03030 at 88.365 kb on - strand, within EX31_RS03030 at 88.429 kb on + strand, within EX31_RS03030 at 88.430 kb on - strand, within EX31_RS03030 at 88.443 kb on + strand, within EX31_RS03030 at 88.444 kb on - strand, within EX31_RS03030 at 88.444 kb on - strand, within EX31_RS03030 at 88.519 kb on + strand, within EX31_RS03030 at 88.519 kb on + strand, within EX31_RS03030 at 88.676 kb on - strand, within EX31_RS03030 at 88.676 kb on - strand, within EX31_RS03030 at 88.793 kb on + strand, within EX31_RS03030 at 88.793 kb on + strand, within EX31_RS03030 at 88.794 kb on - strand, within EX31_RS03030 at 88.794 kb on - strand, within EX31_RS03030 at 88.798 kb on + strand, within EX31_RS03030 at 88.798 kb on + strand, within EX31_RS03030 at 88.798 kb on + strand, within EX31_RS03030 at 88.799 kb on - strand, within EX31_RS03030 at 88.799 kb on - strand, within EX31_RS03030 at 88.799 kb on - strand, within EX31_RS03030 at 88.846 kb on + strand, within EX31_RS03030 at 88.846 kb on + strand, within EX31_RS03030 at 88.847 kb on - strand, within EX31_RS03030 at 88.847 kb on - strand, within EX31_RS03030 at 88.867 kb on - strand, within EX31_RS03030 at 88.881 kb on - strand, within EX31_RS03030 at 88.881 kb on - strand, within EX31_RS03030 at 88.881 kb on - strand, within EX31_RS03030 at 88.903 kb on + strand, within EX31_RS03030 at 88.904 kb on - strand at 88.904 kb on - strand at 88.904 kb on - strand at 88.904 kb on - strand at 88.912 kb on + strand at 88.940 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.973 kb on - strand at 88.973 kb on - strand at 88.974 kb on + strand at 88.974 kb on + strand at 88.974 kb on + strand at 88.974 kb on + strand at 88.974 kb on + strand at 88.975 kb on - strand at 88.975 kb on - strand at 88.975 kb on - strand at 88.975 kb on - strand at 89.011 kb on + strand at 89.011 kb on + strand at 89.011 kb on + strand at 89.011 kb on + strand at 89.011 kb on + strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.012 kb on - strand at 89.023 kb on + strand at 89.023 kb on + strand at 89.024 kb on - strand at 89.024 kb on - strand at 89.024 kb on - strand at 89.024 kb on - strand at 89.024 kb on - strand at 89.024 kb on - strand at 89.050 kb on + strand, within EX31_RS03035 at 89.050 kb on + strand, within EX31_RS03035 at 89.050 kb on + strand, within EX31_RS03035 at 89.050 kb on + strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.051 kb on - strand, within EX31_RS03035 at 89.056 kb on + strand, within EX31_RS03035 at 89.056 kb on + strand, within EX31_RS03035 at 89.057 kb on - strand, within EX31_RS03035 at 89.057 kb on - strand, within EX31_RS03035 at 89.057 kb on - strand, within EX31_RS03035 at 89.057 kb on - strand, within EX31_RS03035 at 89.360 kb on + strand, within EX31_RS03035 at 89.360 kb on + strand, within EX31_RS03035 at 89.361 kb on - strand, within EX31_RS03035 at 89.361 kb on - strand, within EX31_RS03035 at 89.416 kb on + strand, within EX31_RS03035 at 89.417 kb on - strand, within EX31_RS03035 at 89.417 kb on - strand, within EX31_RS03035 at 89.459 kb on - strand, within EX31_RS03035 at 89.463 kb on + strand, within EX31_RS03035 at 89.463 kb on + strand, within EX31_RS03035 at 89.464 kb on - strand, within EX31_RS03035 at 89.464 kb on - strand, within EX31_RS03035 at 89.546 kb on + strand at 89.547 kb on - strand at 89.584 kb on - strand at 89.609 kb on + strand at 89.623 kb on + strand at 89.624 kb on - strand at 89.624 kb on - strand at 89.628 kb on - strand at 89.628 kb on - strand at 89.637 kb on + strand at 89.637 kb on + strand at 89.637 kb on + strand at 89.638 kb on - strand at 89.638 kb on - strand at 89.638 kb on - strand at 89.659 kb on + strand at 89.659 kb on + strand at 89.660 kb on - strand at 89.660 kb on - strand at 89.866 kb on - strand, within EX31_RS03040 at 89.866 kb on - strand, within EX31_RS03040 at 89.866 kb on - strand, within EX31_RS03040 at 89.866 kb on - strand, within EX31_RS03040 at 89.866 kb on - strand, within EX31_RS03040 at 89.946 kb on + strand, within EX31_RS03040 at 89.946 kb on + strand, within EX31_RS03040 at 89.946 kb on + strand, within EX31_RS03040 at 89.947 kb on - strand, within EX31_RS03040 at 89.947 kb on - strand, within EX31_RS03040 at 90.014 kb on + strand, within EX31_RS03040 at 90.014 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.047 kb on + strand, within EX31_RS03040 at 90.048 kb on - strand, within EX31_RS03040 at 90.150 kb on + strand, within EX31_RS03040 at 90.243 kb on + strand, within EX31_RS03040 at 90.243 kb on + strand, within EX31_RS03040 at 90.243 kb on + strand, within EX31_RS03040 at 90.243 kb on + strand, within EX31_RS03040 at 90.244 kb on - strand, within EX31_RS03040 at 90.244 kb on - strand, within EX31_RS03040 at 90.244 kb on - strand, within EX31_RS03040 at 90.244 kb on - strand, within EX31_RS03040 at 90.247 kb on + strand, within EX31_RS03040 at 90.248 kb on - strand, within EX31_RS03040 at 90.248 kb on - strand, within EX31_RS03040 at 90.312 kb on + strand, within EX31_RS03040 at 90.312 kb on + strand, within EX31_RS03040 at 90.330 kb on + strand, within EX31_RS03040 at 90.334 kb on + strand, within EX31_RS03040 at 90.335 kb on - strand, within EX31_RS03040 at 90.375 kb on + strand, within EX31_RS03040 at 90.375 kb on + strand, within EX31_RS03040 at 90.427 kb on - strand, within EX31_RS03040 at 90.431 kb on + strand, within EX31_RS03040 at 90.431 kb on + strand, within EX31_RS03040 at 90.431 kb on + strand, within EX31_RS03040 at 90.431 kb on + strand, within EX31_RS03040 at 90.432 kb on - strand, within EX31_RS03040 at 90.432 kb on - strand, within EX31_RS03040 at 90.432 kb on - strand, within EX31_RS03040 at 90.510 kb on + strand, within EX31_RS03040 at 90.550 kb on + strand, within EX31_RS03040 at 90.602 kb on + strand, within EX31_RS03040 at 90.602 kb on + strand, within EX31_RS03040 at 90.603 kb on - strand, within EX31_RS03040
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 87,981 - +0.3 87,983 + -1.5 87,983 + +0.5 87,984 - +0.6 87,996 + -1.6 87,996 + -0.6 87,996 + -2.0 87,996 + +1.0 87,996 + +0.6 87,996 + +0.6 87,997 - -0.7 87,997 - -0.9 88,032 + -0.8 88,111 - -0.4 88,121 + -0.4 88,123 + +0.5 88,123 + -0.8 88,123 + -1.8 88,123 + +1.2 88,123 + -1.5 88,124 - -0.3 88,138 + +0.0 88,139 - -0.1 88,139 - +0.5 88,190 - -1.7 88,190 - +0.0 88,190 - -0.3 88,210 + -3.7 88,264 + EX31_RS03030 0.11 +2.0 88,264 + EX31_RS03030 0.11 +1.4 88,264 + EX31_RS03030 0.11 -0.4 88,264 + EX31_RS03030 0.11 -0.6 88,264 + EX31_RS03030 0.11 -1.6 88,265 - EX31_RS03030 0.11 -1.1 88,265 - EX31_RS03030 0.11 -3.1 88,265 - EX31_RS03030 0.11 -0.6 88,265 - EX31_RS03030 0.11 -0.7 88,277 + EX31_RS03030 0.12 -1.6 88,277 + EX31_RS03030 0.12 -0.7 88,277 + EX31_RS03030 0.12 -0.8 88,277 + EX31_RS03030 0.12 -0.7 88,277 + EX31_RS03030 0.12 -0.1 88,277 + EX31_RS03030 0.12 -1.5 88,277 + EX31_RS03030 0.12 +1.1 88,277 + EX31_RS03030 0.12 -0.8 88,278 - EX31_RS03030 0.12 +0.2 88,278 - EX31_RS03030 0.12 -0.8 88,278 - EX31_RS03030 0.12 -0.1 88,278 - EX31_RS03030 0.12 +0.9 88,354 + EX31_RS03030 0.22 -1.0 88,364 + EX31_RS03030 0.23 -1.0 88,365 - EX31_RS03030 0.23 -1.0 88,429 + EX31_RS03030 0.31 -1.3 88,430 - EX31_RS03030 0.31 -1.4 88,443 + EX31_RS03030 0.33 -0.3 88,444 - EX31_RS03030 0.33 +0.2 88,444 - EX31_RS03030 0.33 -2.2 88,519 + EX31_RS03030 0.42 +0.0 88,519 + EX31_RS03030 0.42 -1.6 88,676 - EX31_RS03030 0.62 +0.6 88,676 - EX31_RS03030 0.62 -1.5 88,793 + EX31_RS03030 0.76 -0.9 88,793 + EX31_RS03030 0.76 -2.2 88,794 - EX31_RS03030 0.76 -0.3 88,794 - EX31_RS03030 0.76 -1.2 88,798 + EX31_RS03030 0.77 -0.8 88,798 + EX31_RS03030 0.77 -0.9 88,798 + EX31_RS03030 0.77 -3.6 88,799 - EX31_RS03030 0.77 -0.5 88,799 - EX31_RS03030 0.77 -1.8 88,799 - EX31_RS03030 0.77 -1.0 88,846 + EX31_RS03030 0.83 -2.1 88,846 + EX31_RS03030 0.83 -2.1 88,847 - EX31_RS03030 0.83 +0.8 88,847 - EX31_RS03030 0.83 -3.0 88,867 - EX31_RS03030 0.85 +1.2 88,881 - EX31_RS03030 0.87 -2.4 88,881 - EX31_RS03030 0.87 +3.2 88,881 - EX31_RS03030 0.87 +0.4 88,903 + EX31_RS03030 0.90 +0.8 88,904 - +1.2 88,904 - +0.0 88,904 - -1.1 88,904 - -0.8 88,912 + -0.4 88,940 + -0.1 88,972 + -1.5 88,972 + +1.2 88,972 + -0.8 88,972 + -0.0 88,972 + +0.7 88,972 + -0.3 88,972 + +0.7 88,972 + -0.1 88,972 + +0.7 88,972 + -1.1 88,973 - +0.7 88,973 - +1.7 88,974 + -2.3 88,974 + -1.5 88,974 + -0.8 88,974 + -0.3 88,974 + -0.1 88,975 - +0.2 88,975 - -0.7 88,975 - -0.1 88,975 - -0.3 89,011 + -0.6 89,011 + +0.3 89,011 + +4.2 89,011 + +1.6 89,011 + -1.2 89,012 - -2.6 89,012 - +1.0 89,012 - -0.6 89,012 - -0.3 89,012 - -0.9 89,012 - -1.0 89,012 - +1.2 89,023 + -0.6 89,023 + -2.5 89,024 - -1.0 89,024 - -2.5 89,024 - +1.8 89,024 - -1.1 89,024 - -0.9 89,024 - -1.7 89,050 + EX31_RS03035 0.12 -3.3 89,050 + EX31_RS03035 0.12 +0.3 89,050 + EX31_RS03035 0.12 -0.2 89,050 + EX31_RS03035 0.12 +0.7 89,051 - EX31_RS03035 0.13 -2.5 89,051 - EX31_RS03035 0.13 -1.2 89,051 - EX31_RS03035 0.13 -0.2 89,051 - EX31_RS03035 0.13 -2.1 89,051 - EX31_RS03035 0.13 -1.5 89,051 - EX31_RS03035 0.13 -0.7 89,051 - EX31_RS03035 0.13 -1.6 89,051 - EX31_RS03035 0.13 -1.9 89,051 - EX31_RS03035 0.13 -0.9 89,051 - EX31_RS03035 0.13 -2.1 89,051 - EX31_RS03035 0.13 -0.5 89,056 + EX31_RS03035 0.13 -0.5 89,056 + EX31_RS03035 0.13 -2.8 89,057 - EX31_RS03035 0.13 -1.3 89,057 - EX31_RS03035 0.13 -1.7 89,057 - EX31_RS03035 0.13 -0.9 89,057 - EX31_RS03035 0.13 -2.1 89,360 + EX31_RS03035 0.61 -2.8 89,360 + EX31_RS03035 0.61 -1.0 89,361 - EX31_RS03035 0.61 +0.3 89,361 - EX31_RS03035 0.61 -0.3 89,416 + EX31_RS03035 0.70 -1.0 89,417 - EX31_RS03035 0.70 +0.4 89,417 - EX31_RS03035 0.70 +0.9 89,459 - EX31_RS03035 0.76 -1.7 89,463 + EX31_RS03035 0.77 -0.8 89,463 + EX31_RS03035 0.77 -1.1 89,464 - EX31_RS03035 0.77 -0.7 89,464 - EX31_RS03035 0.77 -1.9 89,546 + -0.1 89,547 - -1.7 89,584 - -0.4 89,609 + +0.5 89,623 + -0.9 89,624 - -0.8 89,624 - -0.2 89,628 - -0.4 89,628 - -2.0 89,637 + -0.3 89,637 + -3.8 89,637 + -2.2 89,638 - +0.6 89,638 - +0.7 89,638 - +0.3 89,659 + +0.6 89,659 + -0.6 89,660 - +1.6 89,660 - +0.1 89,866 - EX31_RS03040 0.20 -0.1 89,866 - EX31_RS03040 0.20 +0.2 89,866 - EX31_RS03040 0.20 +0.4 89,866 - EX31_RS03040 0.20 -0.3 89,866 - EX31_RS03040 0.20 -0.7 89,946 + EX31_RS03040 0.26 +1.2 89,946 + EX31_RS03040 0.26 -0.2 89,946 + EX31_RS03040 0.26 -0.4 89,947 - EX31_RS03040 0.26 +0.5 89,947 - EX31_RS03040 0.26 -1.6 90,014 + EX31_RS03040 0.32 +0.6 90,014 + EX31_RS03040 0.32 +1.2 90,047 + EX31_RS03040 0.34 -0.8 90,047 + EX31_RS03040 0.34 -1.3 90,047 + EX31_RS03040 0.34 -2.6 90,047 + EX31_RS03040 0.34 -1.7 90,047 + EX31_RS03040 0.34 -0.9 90,047 + EX31_RS03040 0.34 +1.5 90,048 - EX31_RS03040 0.34 +0.5 90,150 + EX31_RS03040 0.42 +0.3 90,243 + EX31_RS03040 0.49 +1.2 90,243 + EX31_RS03040 0.49 -0.5 90,243 + EX31_RS03040 0.49 -2.2 90,243 + EX31_RS03040 0.49 -0.6 90,244 - EX31_RS03040 0.49 +0.2 90,244 - EX31_RS03040 0.49 -0.6 90,244 - EX31_RS03040 0.49 -2.1 90,244 - EX31_RS03040 0.49 +0.5 90,247 + EX31_RS03040 0.50 -1.5 90,248 - EX31_RS03040 0.50 +1.6 90,248 - EX31_RS03040 0.50 -0.1 90,312 + EX31_RS03040 0.55 +1.2 90,312 + EX31_RS03040 0.55 -2.3 90,330 + EX31_RS03040 0.56 -0.9 90,334 + EX31_RS03040 0.56 -1.9 90,335 - EX31_RS03040 0.56 -2.6 90,375 + EX31_RS03040 0.59 +0.6 90,375 + EX31_RS03040 0.59 -0.1 90,427 - EX31_RS03040 0.63 -0.5 90,431 + EX31_RS03040 0.64 -0.9 90,431 + EX31_RS03040 0.64 -2.3 90,431 + EX31_RS03040 0.64 -3.3 90,431 + EX31_RS03040 0.64 -2.2 90,432 - EX31_RS03040 0.64 -0.3 90,432 - EX31_RS03040 0.64 -0.8 90,432 - EX31_RS03040 0.64 -0.1 90,510 + EX31_RS03040 0.70 -1.9 90,550 + EX31_RS03040 0.73 -0.4 90,602 + EX31_RS03040 0.77 -0.4 90,602 + EX31_RS03040 0.77 +0.6 90,603 - EX31_RS03040 0.77 -0.9
Or see this region's nucleotide sequence