Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03010 and EX31_RS03015 are separated by 98 nucleotides EX31_RS03015 and EX31_RS03020 overlap by 4 nucleotides EX31_RS03020 and EX31_RS03025 are separated by 50 nucleotides
EX31_RS03010: EX31_RS03010 - helix-turn-helix domain-containing protein, at 84,523 to 85,071
_RS03010
EX31_RS03015: EX31_RS03015 - branched-chain amino acid ABC transporter permease, at 85,170 to 85,838
_RS03015
EX31_RS03020: EX31_RS03020 - AzlD domain-containing protein, at 85,835 to 86,158
_RS03020
EX31_RS03025: EX31_RS03025 - APC family permease, at 86,209 to 87,570
_RS03025
Position (kb) 85
86
87 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 84.855 kb on + strand, within EX31_RS03010 at 84.890 kb on - strand, within EX31_RS03010 at 85.051 kb on - strand at 85.056 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.059 kb on + strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.060 kb on - strand at 85.071 kb on + strand at 85.071 kb on + strand at 85.071 kb on + strand at 85.071 kb on + strand at 85.072 kb on - strand at 85.072 kb on - strand at 85.169 kb on + strand at 85.169 kb on + strand at 85.170 kb on - strand at 85.211 kb on + strand at 85.211 kb on + strand at 85.211 kb on + strand at 85.348 kb on + strand, within EX31_RS03015 at 85.348 kb on + strand, within EX31_RS03015 at 85.349 kb on - strand, within EX31_RS03015 at 85.352 kb on + strand, within EX31_RS03015 at 85.352 kb on + strand, within EX31_RS03015 at 85.352 kb on + strand, within EX31_RS03015 at 85.426 kb on + strand, within EX31_RS03015 at 85.427 kb on - strand, within EX31_RS03015 at 85.532 kb on + strand, within EX31_RS03015 at 85.532 kb on + strand, within EX31_RS03015 at 85.532 kb on + strand, within EX31_RS03015 at 85.532 kb on + strand, within EX31_RS03015 at 85.533 kb on - strand, within EX31_RS03015 at 85.558 kb on - strand, within EX31_RS03015 at 85.585 kb on + strand, within EX31_RS03015 at 85.617 kb on + strand, within EX31_RS03015 at 85.617 kb on + strand, within EX31_RS03015 at 85.617 kb on + strand, within EX31_RS03015 at 85.617 kb on + strand, within EX31_RS03015 at 85.618 kb on - strand, within EX31_RS03015 at 85.618 kb on - strand, within EX31_RS03015 at 85.980 kb on + strand, within EX31_RS03020 at 85.980 kb on + strand, within EX31_RS03020 at 85.980 kb on + strand, within EX31_RS03020 at 85.980 kb on + strand, within EX31_RS03020 at 85.980 kb on + strand, within EX31_RS03020 at 85.981 kb on - strand, within EX31_RS03020 at 85.999 kb on + strand, within EX31_RS03020 at 86.104 kb on + strand, within EX31_RS03020 at 86.104 kb on + strand, within EX31_RS03020 at 86.105 kb on - strand, within EX31_RS03020 at 86.105 kb on - strand, within EX31_RS03020 at 86.107 kb on + strand, within EX31_RS03020 at 86.107 kb on + strand, within EX31_RS03020 at 86.210 kb on + strand at 86.211 kb on - strand at 86.211 kb on - strand at 86.270 kb on + strand at 86.270 kb on + strand at 86.270 kb on + strand at 86.270 kb on + strand at 86.271 kb on - strand at 86.271 kb on - strand at 86.271 kb on - strand at 86.271 kb on - strand at 86.275 kb on - strand at 86.378 kb on + strand, within EX31_RS03025 at 86.379 kb on - strand, within EX31_RS03025 at 86.385 kb on + strand, within EX31_RS03025 at 86.385 kb on + strand, within EX31_RS03025 at 86.386 kb on - strand, within EX31_RS03025 at 86.583 kb on + strand, within EX31_RS03025 at 86.584 kb on - strand, within EX31_RS03025 at 86.584 kb on - strand, within EX31_RS03025 at 86.584 kb on - strand, within EX31_RS03025 at 86.586 kb on - strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.630 kb on + strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.631 kb on - strand, within EX31_RS03025 at 86.665 kb on - strand, within EX31_RS03025 at 86.770 kb on - strand, within EX31_RS03025 at 86.782 kb on - strand, within EX31_RS03025 at 86.792 kb on + strand, within EX31_RS03025 at 86.793 kb on - strand, within EX31_RS03025 at 86.793 kb on - strand, within EX31_RS03025 at 86.807 kb on + strand, within EX31_RS03025 at 86.808 kb on - strand, within EX31_RS03025 at 86.808 kb on - strand, within EX31_RS03025 at 86.839 kb on + strand, within EX31_RS03025 at 86.904 kb on + strand, within EX31_RS03025 at 86.904 kb on + strand, within EX31_RS03025 at 86.905 kb on - strand, within EX31_RS03025 at 86.905 kb on - strand, within EX31_RS03025 at 86.970 kb on + strand, within EX31_RS03025 at 87.042 kb on - strand, within EX31_RS03025 at 87.100 kb on - strand, within EX31_RS03025 at 87.100 kb on - strand, within EX31_RS03025 at 87.100 kb on - strand, within EX31_RS03025 at 87.103 kb on - strand, within EX31_RS03025 at 87.103 kb on - strand, within EX31_RS03025 at 87.103 kb on - strand, within EX31_RS03025 at 87.103 kb on - strand, within EX31_RS03025 at 87.103 kb on - strand, within EX31_RS03025 at 87.148 kb on - strand, within EX31_RS03025 at 87.148 kb on - strand, within EX31_RS03025 at 87.148 kb on - strand, within EX31_RS03025 at 87.148 kb on - strand, within EX31_RS03025
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 84,855 + EX31_RS03010 0.60 -1.0 84,890 - EX31_RS03010 0.67 -0.6 85,051 - -0.4 85,056 + -0.2 85,059 + -3.3 85,059 + -0.1 85,059 + -0.6 85,059 + -1.1 85,059 + +0.6 85,059 + -1.0 85,059 + -0.4 85,059 + -0.3 85,059 + -1.7 85,059 + +0.3 85,059 + -0.6 85,059 + +2.0 85,059 + -0.9 85,059 + +0.9 85,059 + +0.2 85,059 + -0.4 85,060 - -1.3 85,060 - -1.0 85,060 - +0.5 85,060 - +0.9 85,060 - +1.0 85,060 - +0.0 85,060 - -1.9 85,060 - -0.3 85,060 - -0.1 85,060 - -0.7 85,060 - -0.5 85,060 - +0.6 85,060 - -1.3 85,060 - -2.0 85,060 - -2.5 85,060 - +1.3 85,060 - -0.7 85,060 - -0.6 85,071 + -0.2 85,071 + +0.1 85,071 + -0.4 85,071 + +0.2 85,072 - +1.1 85,072 - +1.4 85,169 + +1.4 85,169 + +1.5 85,170 - -0.1 85,211 + -0.2 85,211 + +0.3 85,211 + +1.0 85,348 + EX31_RS03015 0.27 +0.1 85,348 + EX31_RS03015 0.27 -0.9 85,349 - EX31_RS03015 0.27 -1.4 85,352 + EX31_RS03015 0.27 -0.7 85,352 + EX31_RS03015 0.27 +0.8 85,352 + EX31_RS03015 0.27 +0.5 85,426 + EX31_RS03015 0.38 -1.0 85,427 - EX31_RS03015 0.38 -0.9 85,532 + EX31_RS03015 0.54 -0.8 85,532 + EX31_RS03015 0.54 -1.8 85,532 + EX31_RS03015 0.54 +1.0 85,532 + EX31_RS03015 0.54 -0.1 85,533 - EX31_RS03015 0.54 -0.7 85,558 - EX31_RS03015 0.58 -0.4 85,585 + EX31_RS03015 0.62 +0.3 85,617 + EX31_RS03015 0.67 +0.2 85,617 + EX31_RS03015 0.67 -0.9 85,617 + EX31_RS03015 0.67 -0.9 85,617 + EX31_RS03015 0.67 -0.2 85,618 - EX31_RS03015 0.67 -0.1 85,618 - EX31_RS03015 0.67 -1.1 85,980 + EX31_RS03020 0.45 -0.2 85,980 + EX31_RS03020 0.45 -0.0 85,980 + EX31_RS03020 0.45 -0.6 85,980 + EX31_RS03020 0.45 -2.3 85,980 + EX31_RS03020 0.45 -0.6 85,981 - EX31_RS03020 0.45 -0.0 85,999 + EX31_RS03020 0.51 -1.3 86,104 + EX31_RS03020 0.83 -2.0 86,104 + EX31_RS03020 0.83 -2.0 86,105 - EX31_RS03020 0.83 -1.5 86,105 - EX31_RS03020 0.83 -2.2 86,107 + EX31_RS03020 0.84 +0.6 86,107 + EX31_RS03020 0.84 -1.1 86,210 + -0.5 86,211 - -1.4 86,211 - +1.3 86,270 + -0.8 86,270 + -0.4 86,270 + -0.3 86,270 + +0.4 86,271 - +0.6 86,271 - +0.6 86,271 - +0.4 86,271 - +0.7 86,275 - -0.1 86,378 + EX31_RS03025 0.12 -0.5 86,379 - EX31_RS03025 0.12 -1.3 86,385 + EX31_RS03025 0.13 -0.5 86,385 + EX31_RS03025 0.13 +0.5 86,386 - EX31_RS03025 0.13 -0.8 86,583 + EX31_RS03025 0.27 +0.3 86,584 - EX31_RS03025 0.28 +1.6 86,584 - EX31_RS03025 0.28 -0.3 86,584 - EX31_RS03025 0.28 -0.4 86,586 - EX31_RS03025 0.28 +0.7 86,630 + EX31_RS03025 0.31 +0.0 86,630 + EX31_RS03025 0.31 -0.5 86,630 + EX31_RS03025 0.31 -1.6 86,630 + EX31_RS03025 0.31 +0.8 86,630 + EX31_RS03025 0.31 +0.1 86,630 + EX31_RS03025 0.31 +0.8 86,630 + EX31_RS03025 0.31 +0.7 86,630 + EX31_RS03025 0.31 -0.3 86,630 + EX31_RS03025 0.31 +1.5 86,630 + EX31_RS03025 0.31 -0.3 86,630 + EX31_RS03025 0.31 -0.4 86,630 + EX31_RS03025 0.31 -0.1 86,630 + EX31_RS03025 0.31 -0.6 86,630 + EX31_RS03025 0.31 -1.2 86,630 + EX31_RS03025 0.31 -0.5 86,631 - EX31_RS03025 0.31 +0.3 86,631 - EX31_RS03025 0.31 +0.6 86,631 - EX31_RS03025 0.31 +0.4 86,631 - EX31_RS03025 0.31 -0.7 86,631 - EX31_RS03025 0.31 -0.2 86,631 - EX31_RS03025 0.31 +0.0 86,631 - EX31_RS03025 0.31 +0.6 86,631 - EX31_RS03025 0.31 +0.0 86,631 - EX31_RS03025 0.31 +0.3 86,631 - EX31_RS03025 0.31 -0.0 86,631 - EX31_RS03025 0.31 -1.7 86,631 - EX31_RS03025 0.31 +0.1 86,631 - EX31_RS03025 0.31 -0.5 86,631 - EX31_RS03025 0.31 +0.1 86,665 - EX31_RS03025 0.33 +0.3 86,770 - EX31_RS03025 0.41 -0.9 86,782 - EX31_RS03025 0.42 -0.1 86,792 + EX31_RS03025 0.43 +2.5 86,793 - EX31_RS03025 0.43 -0.3 86,793 - EX31_RS03025 0.43 +1.2 86,807 + EX31_RS03025 0.44 -0.7 86,808 - EX31_RS03025 0.44 +0.1 86,808 - EX31_RS03025 0.44 +0.6 86,839 + EX31_RS03025 0.46 +0.8 86,904 + EX31_RS03025 0.51 +0.6 86,904 + EX31_RS03025 0.51 -0.1 86,905 - EX31_RS03025 0.51 +0.1 86,905 - EX31_RS03025 0.51 +0.6 86,970 + EX31_RS03025 0.56 -0.5 87,042 - EX31_RS03025 0.61 +0.1 87,100 - EX31_RS03025 0.65 -1.7 87,100 - EX31_RS03025 0.65 +0.5 87,100 - EX31_RS03025 0.65 +0.7 87,103 - EX31_RS03025 0.66 +0.3 87,103 - EX31_RS03025 0.66 -0.1 87,103 - EX31_RS03025 0.66 +0.5 87,103 - EX31_RS03025 0.66 -0.3 87,103 - EX31_RS03025 0.66 +0.5 87,148 - EX31_RS03025 0.69 +1.0 87,148 - EX31_RS03025 0.69 -0.8 87,148 - EX31_RS03025 0.69 +0.5 87,148 - EX31_RS03025 0.69 +1.6
Or see this region's nucleotide sequence