Strain Fitness in Rahnella sp. WP5 around EX31_RS01245

Experiment: L-Serine (N); ShewMM media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS01240 and EX31_RS01245 are separated by 70 nucleotidesEX31_RS01245 and EX31_RS01250 are separated by 220 nucleotides EX31_RS01240: EX31_RS01240 - glycoside hydrolase family 10 protein, at 275,203 to 276,588 _RS01240 EX31_RS01245: EX31_RS01245 - ferric-rhodotorulic acid/ferric-coprogen receptor FhuE, at 276,659 to 278,869 _RS01245 EX31_RS01250: EX31_RS01250 - M24 family metallopeptidase, at 279,090 to 280,484 _RS01250 Position (kb) 276 277 278 279Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 275.700 kb on + strand, within EX31_RS01240at 275.701 kb on - strand, within EX31_RS01240at 275.748 kb on + strand, within EX31_RS01240at 275.892 kb on + strand, within EX31_RS01240at 275.977 kb on + strand, within EX31_RS01240at 276.034 kb on + strand, within EX31_RS01240at 276.035 kb on - strand, within EX31_RS01240at 276.035 kb on - strand, within EX31_RS01240at 276.113 kb on + strand, within EX31_RS01240at 276.113 kb on + strand, within EX31_RS01240at 276.229 kb on + strand, within EX31_RS01240at 276.271 kb on + strand, within EX31_RS01240at 276.271 kb on + strand, within EX31_RS01240at 276.271 kb on + strand, within EX31_RS01240at 276.272 kb on - strand, within EX31_RS01240at 276.273 kb on + strand, within EX31_RS01240at 276.273 kb on + strand, within EX31_RS01240at 276.273 kb on + strand, within EX31_RS01240at 276.273 kb on + strand, within EX31_RS01240at 276.273 kb on + strand, within EX31_RS01240at 276.273 kb on + strand, within EX31_RS01240at 276.274 kb on - strand, within EX31_RS01240at 276.366 kb on + strand, within EX31_RS01240at 276.366 kb on + strand, within EX31_RS01240at 276.376 kb on + strand, within EX31_RS01240at 276.394 kb on + strand, within EX31_RS01240at 276.394 kb on + strand, within EX31_RS01240at 276.394 kb on + strand, within EX31_RS01240at 276.394 kb on + strand, within EX31_RS01240at 276.396 kb on + strand, within EX31_RS01240at 276.591 kb on + strandat 276.599 kb on + strandat 276.599 kb on + strandat 276.599 kb on + strandat 276.657 kb on + strandat 276.714 kb on + strandat 276.732 kb on + strandat 276.735 kb on + strandat 276.735 kb on + strandat 276.735 kb on + strandat 276.736 kb on - strandat 276.736 kb on - strandat 276.736 kb on - strandat 276.736 kb on - strandat 276.736 kb on - strandat 276.795 kb on + strandat 276.896 kb on + strand, within EX31_RS01245at 276.973 kb on + strand, within EX31_RS01245at 276.973 kb on + strand, within EX31_RS01245at 276.973 kb on + strand, within EX31_RS01245at 277.014 kb on + strand, within EX31_RS01245at 277.014 kb on + strand, within EX31_RS01245at 277.014 kb on + strand, within EX31_RS01245at 277.110 kb on + strand, within EX31_RS01245at 277.185 kb on - strand, within EX31_RS01245at 277.201 kb on + strand, within EX31_RS01245at 277.229 kb on - strand, within EX31_RS01245at 277.269 kb on + strand, within EX31_RS01245at 277.269 kb on + strand, within EX31_RS01245at 277.270 kb on - strand, within EX31_RS01245at 277.315 kb on + strand, within EX31_RS01245at 277.316 kb on - strand, within EX31_RS01245at 277.316 kb on - strand, within EX31_RS01245at 277.316 kb on - strand, within EX31_RS01245at 277.316 kb on - strand, within EX31_RS01245at 277.317 kb on + strand, within EX31_RS01245at 277.354 kb on - strand, within EX31_RS01245at 277.426 kb on + strand, within EX31_RS01245at 277.427 kb on - strand, within EX31_RS01245at 277.428 kb on + strand, within EX31_RS01245at 277.428 kb on + strand, within EX31_RS01245at 277.524 kb on + strand, within EX31_RS01245at 277.617 kb on + strand, within EX31_RS01245at 277.641 kb on + strand, within EX31_RS01245at 277.642 kb on - strand, within EX31_RS01245at 277.797 kb on + strand, within EX31_RS01245at 277.911 kb on + strand, within EX31_RS01245at 277.939 kb on + strand, within EX31_RS01245at 277.940 kb on - strand, within EX31_RS01245at 278.272 kb on + strand, within EX31_RS01245at 278.424 kb on + strand, within EX31_RS01245at 278.425 kb on - strand, within EX31_RS01245at 278.425 kb on - strand, within EX31_RS01245at 278.434 kb on + strand, within EX31_RS01245at 278.434 kb on + strand, within EX31_RS01245at 278.435 kb on - strand, within EX31_RS01245at 278.442 kb on + strand, within EX31_RS01245at 278.443 kb on - strand, within EX31_RS01245at 278.443 kb on - strand, within EX31_RS01245at 278.460 kb on + strand, within EX31_RS01245at 278.460 kb on + strand, within EX31_RS01245at 278.461 kb on - strand, within EX31_RS01245at 278.509 kb on + strand, within EX31_RS01245at 278.509 kb on + strand, within EX31_RS01245at 278.807 kb on - strandat 278.847 kb on + strandat 278.848 kb on - strandat 278.848 kb on - strandat 278.848 kb on - strandat 278.848 kb on - strandat 278.869 kb on + strandat 278.870 kb on - strandat 278.870 kb on - strandat 278.912 kb on + strandat 278.917 kb on - strandat 278.917 kb on - strandat 278.917 kb on - strandat 279.005 kb on + strandat 279.013 kb on + strandat 279.040 kb on + strandat 279.116 kb on + strandat 279.213 kb on - strandat 279.293 kb on + strand, within EX31_RS01250at 279.294 kb on - strand, within EX31_RS01250at 279.336 kb on - strand, within EX31_RS01250at 279.336 kb on - strand, within EX31_RS01250at 279.339 kb on - strand, within EX31_RS01250at 279.388 kb on + strand, within EX31_RS01250at 279.388 kb on + strand, within EX31_RS01250at 279.452 kb on - strand, within EX31_RS01250at 279.452 kb on - strand, within EX31_RS01250at 279.452 kb on - strand, within EX31_RS01250at 279.534 kb on + strand, within EX31_RS01250at 279.547 kb on + strand, within EX31_RS01250at 279.548 kb on - strand, within EX31_RS01250at 279.548 kb on - strand, within EX31_RS01250at 279.595 kb on - strand, within EX31_RS01250at 279.619 kb on + strand, within EX31_RS01250at 279.620 kb on - strand, within EX31_RS01250at 279.671 kb on + strand, within EX31_RS01250at 279.780 kb on + strand, within EX31_RS01250

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking
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275,700 + EX31_RS01240 0.36 -1.0
275,701 - EX31_RS01240 0.36 -1.8
275,748 + EX31_RS01240 0.39 +0.3
275,892 + EX31_RS01240 0.50 +0.7
275,977 + EX31_RS01240 0.56 +0.9
276,034 + EX31_RS01240 0.60 +1.5
276,035 - EX31_RS01240 0.60 -0.2
276,035 - EX31_RS01240 0.60 +0.6
276,113 + EX31_RS01240 0.66 +0.6
276,113 + EX31_RS01240 0.66 +0.4
276,229 + EX31_RS01240 0.74 -0.2
276,271 + EX31_RS01240 0.77 +0.8
276,271 + EX31_RS01240 0.77 +1.7
276,271 + EX31_RS01240 0.77 +0.6
276,272 - EX31_RS01240 0.77 -1.2
276,273 + EX31_RS01240 0.77 +0.3
276,273 + EX31_RS01240 0.77 +0.9
276,273 + EX31_RS01240 0.77 -0.4
276,273 + EX31_RS01240 0.77 +0.5
276,273 + EX31_RS01240 0.77 +0.0
276,273 + EX31_RS01240 0.77 +1.2
276,274 - EX31_RS01240 0.77 +0.6
276,366 + EX31_RS01240 0.84 +2.5
276,366 + EX31_RS01240 0.84 -3.3
276,376 + EX31_RS01240 0.85 +0.2
276,394 + EX31_RS01240 0.86 -1.7
276,394 + EX31_RS01240 0.86 +0.7
276,394 + EX31_RS01240 0.86 -0.4
276,394 + EX31_RS01240 0.86 +0.2
276,396 + EX31_RS01240 0.86 +0.7
276,591 + +0.5
276,599 + +0.6
276,599 + -0.5
276,599 + -0.5
276,657 + +0.5
276,714 + +0.6
276,732 + -1.1
276,735 + +0.9
276,735 + +2.2
276,735 + +0.6
276,736 - +1.9
276,736 - -1.3
276,736 - +0.5
276,736 - -0.8
276,736 - -1.2
276,795 + -0.2
276,896 + EX31_RS01245 0.11 -0.4
276,973 + EX31_RS01245 0.14 +1.2
276,973 + EX31_RS01245 0.14 +0.3
276,973 + EX31_RS01245 0.14 -0.1
277,014 + EX31_RS01245 0.16 +0.2
277,014 + EX31_RS01245 0.16 -0.2
277,014 + EX31_RS01245 0.16 -0.4
277,110 + EX31_RS01245 0.20 +1.8
277,185 - EX31_RS01245 0.24 +1.2
277,201 + EX31_RS01245 0.25 -0.2
277,229 - EX31_RS01245 0.26 -2.7
277,269 + EX31_RS01245 0.28 -2.2
277,269 + EX31_RS01245 0.28 -0.3
277,270 - EX31_RS01245 0.28 -0.5
277,315 + EX31_RS01245 0.30 -1.8
277,316 - EX31_RS01245 0.30 +0.0
277,316 - EX31_RS01245 0.30 +2.7
277,316 - EX31_RS01245 0.30 -1.1
277,316 - EX31_RS01245 0.30 -1.8
277,317 + EX31_RS01245 0.30 +0.6
277,354 - EX31_RS01245 0.31 -0.8
277,426 + EX31_RS01245 0.35 +2.6
277,427 - EX31_RS01245 0.35 +1.6
277,428 + EX31_RS01245 0.35 +2.2
277,428 + EX31_RS01245 0.35 -1.0
277,524 + EX31_RS01245 0.39 -0.3
277,617 + EX31_RS01245 0.43 +1.6
277,641 + EX31_RS01245 0.44 +0.2
277,642 - EX31_RS01245 0.44 -0.4
277,797 + EX31_RS01245 0.51 -0.8
277,911 + EX31_RS01245 0.57 +0.1
277,939 + EX31_RS01245 0.58 +1.2
277,940 - EX31_RS01245 0.58 +0.9
278,272 + EX31_RS01245 0.73 +0.6
278,424 + EX31_RS01245 0.80 +0.5
278,425 - EX31_RS01245 0.80 -1.0
278,425 - EX31_RS01245 0.80 +2.7
278,434 + EX31_RS01245 0.80 +0.2
278,434 + EX31_RS01245 0.80 +0.2
278,435 - EX31_RS01245 0.80 -0.2
278,442 + EX31_RS01245 0.81 +1.0
278,443 - EX31_RS01245 0.81 +0.3
278,443 - EX31_RS01245 0.81 +1.6
278,460 + EX31_RS01245 0.81 -0.2
278,460 + EX31_RS01245 0.81 +0.3
278,461 - EX31_RS01245 0.82 +0.8
278,509 + EX31_RS01245 0.84 +3.2
278,509 + EX31_RS01245 0.84 +0.4
278,807 - +0.2
278,847 + +1.5
278,848 - +1.4
278,848 - -0.4
278,848 - +1.2
278,848 - +1.2
278,869 + +0.7
278,870 - +0.2
278,870 - -0.3
278,912 + -1.4
278,917 - +1.2
278,917 - +0.7
278,917 - -1.1
279,005 + +0.3
279,013 + -1.2
279,040 + -1.3
279,116 + +1.2
279,213 - +1.0
279,293 + EX31_RS01250 0.15 -0.9
279,294 - EX31_RS01250 0.15 +1.6
279,336 - EX31_RS01250 0.18 +4.2
279,336 - EX31_RS01250 0.18 +1.2
279,339 - EX31_RS01250 0.18 +0.1
279,388 + EX31_RS01250 0.21 -2.8
279,388 + EX31_RS01250 0.21 +0.2
279,452 - EX31_RS01250 0.26 +0.8
279,452 - EX31_RS01250 0.26 -1.3
279,452 - EX31_RS01250 0.26 +0.0
279,534 + EX31_RS01250 0.32 -1.2
279,547 + EX31_RS01250 0.33 +2.2
279,548 - EX31_RS01250 0.33 -0.4
279,548 - EX31_RS01250 0.33 -1.6
279,595 - EX31_RS01250 0.36 +1.5
279,619 + EX31_RS01250 0.38 +0.0
279,620 - EX31_RS01250 0.38 +1.6
279,671 + EX31_RS01250 0.42 +2.0
279,780 + EX31_RS01250 0.49 +0.6

Or see this region's nucleotide sequence